##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549290_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1763616
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41025370602217	31.0	31.0	33.0	30.0	34.0
2	31.83025726688803	31.0	31.0	34.0	30.0	34.0
3	31.96714137317874	33.0	31.0	34.0	30.0	34.0
4	35.70547500136084	37.0	35.0	37.0	33.0	37.0
5	35.613978326347684	37.0	35.0	37.0	33.0	37.0
6	35.66524969154283	37.0	35.0	37.0	33.0	37.0
7	35.963997264710684	37.0	35.0	37.0	35.0	37.0
8	35.9894086921416	37.0	35.0	37.0	35.0	37.0
9	37.82393956507539	39.0	38.0	39.0	35.0	39.0
10	37.28695532360786	39.0	37.0	39.0	34.0	39.0
11	36.968502213633805	39.0	37.0	39.0	33.0	39.0
12	36.0306807150763	37.0	35.0	39.0	32.0	39.0
13	35.668705092264986	37.0	35.0	39.0	31.0	39.0
14	36.619467616533306	38.0	35.0	41.0	31.0	41.0
15	36.94040993050641	38.0	35.0	41.0	32.0	41.0
16	37.0968300355633	38.0	35.0	41.0	33.0	41.0
17	37.01766144103932	38.0	35.0	41.0	32.0	41.0
18	36.94714098760728	38.0	35.0	41.0	32.0	41.0
19	36.88641915246856	38.0	35.0	41.0	32.0	41.0
20	36.7119389935224	38.0	35.0	40.0	32.0	41.0
21	36.51397242937238	38.0	35.0	40.0	31.0	41.0
22	36.42642616079691	38.0	35.0	40.0	31.0	41.0
23	36.428390874203906	38.0	35.0	40.0	31.0	41.0
24	36.33642584326747	37.0	35.0	40.0	31.0	41.0
25	36.20995443452543	37.0	35.0	40.0	31.0	41.0
26	36.07318259757226	37.0	35.0	40.0	31.0	41.0
27	36.02590813419701	37.0	35.0	40.0	31.0	41.0
28	36.08505706457642	37.0	35.0	40.0	31.0	41.0
29	36.161124076896556	37.0	35.0	40.0	31.0	41.0
30	36.22899429354236	37.0	35.0	40.0	31.0	41.0
31	36.08877556111988	37.0	35.0	40.0	31.0	41.0
32	35.93222220710177	37.0	35.0	40.0	30.0	41.0
33	35.79157991308766	37.0	35.0	40.0	30.0	41.0
34	35.7421592909114	37.0	35.0	40.0	30.0	41.0
35	35.58166743780959	37.0	35.0	40.0	30.0	41.0
36	35.43837207192495	37.0	35.0	40.0	29.0	41.0
37	35.37570196686807	37.0	34.0	40.0	29.0	41.0
38	35.34742483624553	37.0	34.0	40.0	29.0	41.0
39	35.32429508464428	37.0	34.0	40.0	29.0	41.0
40	35.21819659154827	36.0	34.0	40.0	28.0	41.0
41	35.18766046577033	36.0	34.0	40.0	29.0	41.0
42	35.14960966559614	36.0	34.0	40.0	28.0	41.0
43	35.097492878268284	36.0	34.0	40.0	28.0	41.0
44	34.98436734527244	36.0	34.0	40.0	27.0	41.0
45	34.88055222905667	36.0	34.0	40.0	27.0	41.0
46	34.84593528296409	36.0	34.0	40.0	27.0	41.0
47	34.82220789559632	35.0	34.0	40.0	27.0	41.0
48	34.74236625206394	35.0	34.0	40.0	27.0	41.0
49	34.69823873224103	36.0	34.0	40.0	27.0	41.0
50	34.60337964727015	35.0	34.0	40.0	27.0	41.0
51	34.17276606698964	35.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	4.0
12	0.0
13	4.0
14	23.0
15	22.0
16	78.0
17	225.0
18	517.0
19	1030.0
20	1947.0
21	3261.0
22	5238.0
23	7829.0
24	11609.0
25	17340.0
26	23541.0
27	28463.0
28	31319.0
29	35945.0
30	42971.0
31	52849.0
32	67696.0
33	90723.0
34	168269.0
35	290003.0
36	114189.0
37	140768.0
38	215554.0
39	411991.0
40	206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.08646326637998	24.60076343149529	29.503984994465917	13.808788307658808
2	29.950964382269156	26.71806107451962	30.00477428192985	13.326200261281368
3	29.43628318182643	25.90297434362129	30.86017591130949	13.800566563242791
4	25.68694092137971	28.293914321484948	30.916877596937205	15.102267160198139
5	23.521163337143687	32.04518443924301	30.3629588300401	14.070693393573205
6	21.651481955255566	41.63326937383194	26.207348992070834	10.507899678841651
7	88.5272077368316	4.078608948886832	5.950785204942573	1.4433981093389943
8	89.10250303921035	3.5187365049988206	5.605982254640466	1.7727782011503639
9	84.04856839584127	6.3289854480794006	7.189263422423021	2.4331827336563063
10	48.89454393700216	29.557171175584706	12.004880881098835	9.5434040063143
11	44.45956489394517	19.849785894435072	22.274293270190338	13.416355941429428
12	42.380200678605775	19.957065483642698	24.258398653675176	13.404335184076352
13	22.07583737049335	38.778793115961754	24.146696332988586	14.998673180556313
14	15.099432075916752	41.945525556583746	26.66067896866438	16.29436339883512
15	14.09632255547693	25.15071308039845	46.36184974506922	14.391114619055395
16	16.437818663473227	20.878524576778617	45.363786674650264	17.31987008509789
17	16.2834766751946	21.859520439823633	28.599252898590173	33.257749986391595
18	21.586048210041188	25.209172518280624	33.49017019577958	19.714609075898608
19	27.709716854462652	26.72004563351659	26.288375700832834	19.281861811187923
20	30.879170975994775	23.81130586250068	26.92660987425834	18.382913287246204
21	22.108667646471794	28.75716709306334	28.918143178560413	20.21602208190445
22	21.772823562498868	25.067701812639488	27.61287037541052	25.54660424945113
23	18.957187959283655	29.983397746448205	26.16170413514053	24.897710159127612
24	20.631872244298076	24.318785948868687	37.92838123491735	17.120960571915884
25	17.43049507375755	26.563435577812854	34.82390724511458	21.182162103315008
26	16.620851704679477	35.92329622775026	27.47168317819752	19.984168889372743
27	17.107068658937095	35.658329250811974	30.0580738664199	17.17652822383104
28	14.640942245931088	30.224550015422857	38.78894271768911	16.345565020956943
29	15.031333351477874	25.287817756246255	38.355968646235915	21.324880246039953
30	17.878154881788326	32.055447444341624	32.11141200805618	17.954985665813876
31	28.584113548527572	28.68016620398091	25.158311106272567	17.57740914121895
32	28.247985956126502	28.505411608876308	27.22208235806434	16.024520076932845
33	26.243411264130064	30.22727169633299	24.617773937183603	18.91154310235335
34	19.45327100684049	29.89318536461452	27.167478634804855	23.486064993740133
35	19.336578937818665	28.003715094442327	30.475114764211707	22.184591203527297
36	29.340343929744346	26.109028269192386	27.31507312249379	17.235554678569486
37	19.12757652459492	33.245785930724146	30.84429943933373	16.782338105347197
38	19.330625260827755	32.89582312702992	25.172770036107632	22.60078157603469
39	19.645773229546567	32.36781703046468	28.95040643768258	19.03600330230617
40	23.935028940540345	28.321357937328763	26.28656124689275	21.457051875238147
41	17.228353564494768	25.28793115961751	29.693935641318745	27.78977963456898
42	21.5842337561011	25.399803585360985	26.575399633480306	26.440563025057607
43	22.282741821348864	26.018872589044328	27.112477999745977	24.58590758986083
44	19.048024059659248	31.486162520639414	28.575778400740298	20.890035018961044
45	17.499784533594614	38.83793297407146	22.98073957142598	20.68154292090795
46	21.933289332825286	34.48097545043819	24.8843852630051	18.701349953731423
47	20.470669352058497	28.894668680710538	28.140819770290133	22.49384219694083
48	23.036137118284252	25.308627274871625	31.591117340736307	20.064118266107815
49	20.48767985774681	24.35065229619146	32.61027343820877	22.551394407852957
50	19.11204026273293	32.54994284470089	29.02854136047757	19.30947553208862
51	18.339990111226026	33.59263014170885	25.575862319235025	22.491517427830093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	758.0
1	1554.0
2	2350.0
3	7933.5
4	13517.0
5	9990.5
6	6464.0
7	6490.0
8	6516.0
9	7073.0
10	7630.0
11	7614.0
12	7598.0
13	7513.5
14	7429.0
15	6823.5
16	6218.0
17	5782.0
18	5346.0
19	5536.0
20	5726.0
21	5942.5
22	6159.0
23	6857.5
24	7556.0
25	9933.5
26	13761.5
27	15212.0
28	21167.0
29	27122.0
30	32354.0
31	37586.0
32	40804.0
33	44022.0
34	49724.5
35	55427.0
36	58412.5
37	61398.0
38	71607.0
39	81816.0
40	111081.5
41	140347.0
42	164363.5
43	188380.0
44	190245.0
45	192110.0
46	186676.0
47	181242.0
48	166948.5
49	152655.0
50	135039.0
51	117423.0
52	105815.5
53	94208.0
54	83755.5
55	73303.0
56	62851.0
57	52399.0
58	48817.0
59	45235.0
60	40327.5
61	35420.0
62	30581.0
63	25742.0
64	21222.5
65	16703.0
66	13764.0
67	10825.0
68	9000.0
69	7175.0
70	6184.5
71	5194.0
72	4322.5
73	3451.0
74	2626.5
75	1386.0
76	970.0
77	785.5
78	601.0
79	394.5
80	188.0
81	114.5
82	41.0
83	31.0
84	21.0
85	17.0
86	13.0
87	7.5
88	2.0
89	1.5
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1763616.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.687703326016353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.73411194860974	12.351573898871488
2	8.186249738195851	2.568469144909911
3	2.6727295100343538	1.2578696287232396
4	1.1616075389619525	0.7289181780999635
5	0.7222508890641323	0.5665228837294827
6	0.48766046532569324	0.4590163622313936
7	0.3557403587296968	0.390652444618948
8	0.2671685434360404	0.33530086779748125
9	0.22725275777192225	0.3208566459550476
>10	3.440842182730835	14.099459398462752
>50	1.9131792861538994	21.64557805094104
>100	1.8231559118448597	43.420211051369755
>500	0.004757331765151176	0.45708990283500606
>1k	0.00292758877855457	0.9497733424393797
>5k	3.6594859731932123E-4	0.44870819901512277
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7816	0.44318037486618406	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4815	0.2730186162974253	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3491	0.19794558452633682	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	1778	0.10081559704606899	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.670168562771034E-5	0.0	0.0	0.03135603215212382	0.0
2	5.670168562771034E-5	0.0	0.0	0.11419719485420862	0.0
3	5.670168562771034E-5	0.0	0.0	0.17900722152668155	0.0
4	5.670168562771034E-5	0.0	0.0	0.3904478072324134	0.0
5	5.670168562771034E-5	0.0	0.0	0.6472497414403136	0.0
6	5.670168562771034E-5	0.0	0.0	0.9820731950719431	0.0
7	5.670168562771034E-5	0.0	0.0	1.1242243209406129	0.0
8	5.670168562771034E-5	0.0	0.0	1.53117231869069	0.0
9	5.670168562771034E-5	0.0	0.0	1.6661223304846406	0.0
10	5.670168562771034E-5	0.0	0.0	1.9254758405457877	0.0
11	5.670168562771034E-5	0.0	0.0	2.2408506160071124	0.0
12	5.670168562771034E-5	0.0	0.0	2.496178306388692	0.0
13	5.670168562771034E-5	0.0	0.0	2.600169197829913	0.0
14	5.670168562771034E-5	0.0	0.0	2.6402005878830765	0.0
15	5.670168562771034E-5	0.0	0.0	2.701608513417887	0.0
16	5.670168562771034E-5	0.0	0.0	2.8451771814272493	0.0
17	5.670168562771034E-5	0.0	0.0	3.010122384918259	0.0
18	5.670168562771034E-5	0.0	0.0	3.2312022571807013	0.0
19	5.670168562771034E-5	0.0	0.0	3.343131384609802	0.0
20	5.670168562771034E-5	0.0	0.0	3.472864841326003	0.0
21	5.670168562771034E-5	0.0	0.0	3.644670948777965	0.0
22	5.670168562771034E-5	0.0	0.0	3.8167038629724384	0.0
23	1.1340337125542068E-4	0.0	0.0	3.9973554333823236	0.0
24	1.1340337125542068E-4	0.0	0.0	4.138032315424673	0.0
25	1.1340337125542068E-4	0.0	0.0	4.256141926587193	0.0
26	1.1340337125542068E-4	0.0	0.0	4.369601999528242	0.0
27	1.1340337125542068E-4	0.0	0.0	4.478242429190935	0.0
28	1.1340337125542068E-4	0.0	0.0	4.5990737212635855	0.0
29	1.1340337125542068E-4	0.0	0.0	4.725915392012774	0.0
30	1.1340337125542068E-4	0.0	0.0	4.888535826393047	0.0
31	1.1340337125542068E-4	0.0	0.0	5.053821239997823	0.0
32	1.1340337125542068E-4	0.0	0.0	5.193817701812639	0.0
33	1.1340337125542068E-4	0.0	0.0	5.342773029956635	0.0
34	1.1340337125542068E-4	0.0	0.0	5.5034089053399375	0.0
35	1.1340337125542068E-4	0.0	0.0	5.699029720755538	0.0
36	1.1340337125542068E-4	0.0	0.0	5.866866710213562	0.0
37	1.1340337125542068E-4	0.0	0.0	6.042301725545697	0.0
38	1.1340337125542068E-4	0.0	0.0	6.207133525665451	0.0
39	1.1340337125542068E-4	0.0	0.0	6.377125179177327	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCCG	20	7.0343126E-4	45.000004	1
TCGATAG	80	0.0	45.000004	1
ATCGTAC	20	7.0343126E-4	45.000004	31
CCGTATC	20	7.0343126E-4	45.000004	25
TACGCAC	20	7.0343126E-4	45.000004	21
GTTCGAC	20	7.0343126E-4	45.000004	28
TTACGCG	40	6.8193913E-9	45.000004	1
CGCACAT	20	7.0343126E-4	45.000004	31
TGTAACG	55	1.8189894E-12	45.000004	30
GCGTAAG	110	0.0	45.000004	1
TACGTTG	40	6.8193913E-9	45.000004	1
AAATGCG	55	1.8189894E-12	45.000004	1
CGAACCG	25	3.891657E-5	45.0	2
TACCACG	25	3.891657E-5	45.0	26
TACGCGG	70	0.0	45.0	2
TTAACGC	25	3.891657E-5	45.0	20
ACCGTAC	25	3.891657E-5	45.0	29
ACGTAAC	25	3.891657E-5	45.0	40
ATACGAC	35	1.2124292E-7	45.0	37
TATACGA	35	1.2124292E-7	45.0	36
>>END_MODULE