FastQCFastQC Report
Sat 18 Jun 2016
SRR3549289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340538
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75010.5595514636660803No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33420.24930289182402884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG551.8189894E-1245.0000041
CGAGTTA302.1657725E-645.00000437
TTTCGCG253.8911538E-545.0000041
GGCGTGA253.8911538E-545.0000048
CCGAGTT302.1657725E-645.00000436
TCCACGA302.1657725E-645.00000423
TCGTAAG600.045.0000041
ACCGTTC302.1657725E-645.00000427
CGCACTC253.8911538E-545.00000412
CGCGTCC253.8911538E-545.00000423
CCGCAAT502.1827873E-1145.00000433
TTGATCG207.033707E-445.020
CGGGTAC207.033707E-445.06
CTATGCG207.033707E-445.01
GTCGACG207.033707E-445.013
CGCGGAT351.2121382E-745.020
ATAGCGC207.033707E-445.012
GCACGAG207.033707E-445.01
AGCGGTG207.033707E-445.041
AAACGCG207.033707E-445.01