FastQCFastQC Report
Sat 18 Jun 2016
SRR3549288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742606
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186950.6816509553322643No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC42890.15638411058679227No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT36910.13458003081740505No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGT207.0349994E-445.017
TCGGTCG253.892227E-544.9999961
TAGTCCG253.892227E-544.9999961
CGTTTTT93550.042.210051
CGACGGT3500.041.14285728
TAGCACG1500.040.5000041
TAGCGAG2400.040.3124961
TTAAGCG1250.039.6000021
AACGACG1250.039.60000213
TCGTAAG800.039.3751
GTGCGTA403.4605546E-739.37516
TTCGCGG2250.039.02
TCGCGCA3750.039.034
TAGGGAC36800.038.947015
AGGGCGA20950.038.8782846
TACGGGA11000.038.6590924
CTATGCG1050.038.571431
CCTAGCG700.038.5714261
TACGCTA356.251459E-638.57142642
CTATTCG356.251459E-638.57142642
Produced by FastQC (version 0.11.5)