##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549288_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2742606 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.311485134941 31.0 31.0 33.0 30.0 34.0 2 31.726739823365076 31.0 31.0 34.0 30.0 34.0 3 31.855010891101383 31.0 31.0 34.0 30.0 34.0 4 35.625265167508566 37.0 35.0 37.0 33.0 37.0 5 35.5267355208878 37.0 35.0 37.0 33.0 37.0 6 35.57477887819103 37.0 35.0 37.0 33.0 37.0 7 35.93314788927028 37.0 35.0 37.0 35.0 37.0 8 35.969696704521176 37.0 35.0 37.0 35.0 37.0 9 37.80530415232811 39.0 38.0 39.0 35.0 39.0 10 37.170116305440885 39.0 37.0 39.0 33.0 39.0 11 36.813867175963296 39.0 37.0 39.0 32.0 39.0 12 36.386752599534894 38.0 35.0 39.0 32.0 39.0 13 36.246182280648405 38.0 35.0 39.0 32.0 39.0 14 37.316907714779305 39.0 35.0 41.0 32.0 41.0 15 37.51402352361222 39.0 35.0 41.0 32.0 41.0 16 37.58245041394936 39.0 35.0 41.0 33.0 41.0 17 37.48074604956016 39.0 35.0 41.0 32.0 41.0 18 37.39591687613897 39.0 36.0 41.0 32.0 41.0 19 37.35656999218991 39.0 36.0 41.0 32.0 41.0 20 37.20693712476382 39.0 35.0 41.0 32.0 41.0 21 37.04775312239527 39.0 35.0 41.0 32.0 41.0 22 36.97713670866322 39.0 35.0 41.0 31.0 41.0 23 36.912522979968685 39.0 35.0 41.0 31.0 41.0 24 36.83849958761849 38.0 35.0 41.0 31.0 41.0 25 36.755970781074645 38.0 35.0 41.0 31.0 41.0 26 36.6548767850723 38.0 35.0 40.0 31.0 41.0 27 36.58832110773476 38.0 35.0 40.0 31.0 41.0 28 36.59074580891313 38.0 35.0 40.0 31.0 41.0 29 36.608007858219516 38.0 35.0 40.0 31.0 41.0 30 36.62898207033748 38.0 35.0 41.0 31.0 41.0 31 36.566105740306845 38.0 35.0 41.0 31.0 41.0 32 36.4445417971083 38.0 35.0 41.0 30.0 41.0 33 36.33892545994576 38.0 35.0 41.0 30.0 41.0 34 36.256335397793194 38.0 35.0 41.0 30.0 41.0 35 36.11016493072647 38.0 35.0 41.0 30.0 41.0 36 36.02056985217709 38.0 35.0 41.0 30.0 41.0 37 35.99346351608653 38.0 35.0 41.0 30.0 41.0 38 35.92511137217668 38.0 35.0 41.0 29.0 41.0 39 35.864745063636555 38.0 35.0 41.0 29.0 41.0 40 35.80201786184381 38.0 35.0 40.0 29.0 41.0 41 35.736319763028305 38.0 35.0 40.0 29.0 41.0 42 35.695273035937355 38.0 35.0 40.0 28.0 41.0 43 35.662486335988476 38.0 35.0 40.0 28.0 41.0 44 35.54435708227868 38.0 34.0 40.0 28.0 41.0 45 35.481780467190696 38.0 34.0 40.0 27.0 41.0 46 35.43330029905863 38.0 34.0 40.0 27.0 41.0 47 35.39257953931407 38.0 34.0 40.0 27.0 41.0 48 35.26662779852447 38.0 34.0 40.0 27.0 41.0 49 35.21364643700189 38.0 34.0 40.0 27.0 41.0 50 35.14164338588919 37.0 34.0 40.0 27.0 41.0 51 34.74601419234115 37.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 0.0 11 4.0 12 5.0 13 5.0 14 10.0 15 32.0 16 112.0 17 339.0 18 753.0 19 1562.0 20 2889.0 21 4742.0 22 7370.0 23 11490.0 24 17165.0 25 25149.0 26 34413.0 27 41007.0 28 46594.0 29 53000.0 30 63395.0 31 77849.0 32 97925.0 33 125954.0 34 202854.0 35 305599.0 36 199201.0 37 258045.0 38 399686.0 39 765188.0 40 267.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.005146200365637 24.373825478395368 30.526222140548075 14.094806180690918 2 29.977036439065618 25.86116999671116 30.667875735705387 13.49391782851784 3 27.813838371242532 26.17670930494573 32.711151364796834 13.298300959014894 4 25.113012951915074 28.283792859783723 32.25111445100025 14.352079737300945 5 23.006840938873466 32.17738895050912 30.72373501698749 14.092035093629926 6 21.249278970439065 41.55044508762833 27.022875323688496 10.177400618244107 7 89.66924158993308 3.13202115068661 5.907957614035702 1.2907796453446103 8 90.6642441531886 2.374930996286014 5.56653051878396 1.3942943317414167 9 85.86523182695582 5.450254247237846 6.677736430241894 2.0067774955644375 10 42.372035939540716 37.06613345117746 12.00934439726304 8.552486212018788 11 30.32571940701654 24.349104464877566 29.60454399939328 15.72063212871262 12 32.258297400355715 22.765646979551565 28.715207361174006 16.260848258918706 13 22.20413723298206 31.484617185261026 28.254441213940318 18.056804367816596 14 16.54692653629431 33.539268855971294 29.528958953637524 20.38484565409687 15 15.459566558229653 28.450203930130684 38.74216712134371 17.348062390295947 16 18.630127696067174 25.17824288286396 38.25890412257539 17.932725298493477 17 18.40483102567412 26.03968634211403 29.69686495253055 25.858617679681295 18 20.029417276852747 27.432339898621965 32.85404465679722 19.684198167728066 19 23.95404225032688 28.252727515363123 28.325614397401594 19.4676158369084 20 26.734463499314153 26.29294911481999 29.652272327851687 17.320315058014167 21 22.720799123169716 28.162594262537166 30.377385596035307 18.73922101825782 22 22.299010503149194 25.264766430176262 29.846613038839703 22.58961002783484 23 19.070876385452376 29.599512288677264 29.959060834841022 21.370550491029334 24 18.9901867056369 26.484555200418875 36.37288768419525 18.152370409748976 25 18.27994979957019 29.82524649913258 31.682020676684875 20.212783024612357 26 17.848243604805063 34.02118277288098 28.202483331546713 19.928090290767248 27 17.724273920497513 33.58116331693287 30.306394720933298 18.388168041636312 28 16.28345449546891 30.572090923741875 34.985922148496726 18.1585324322925 29 17.043753276992756 26.316503354838428 35.449678152822536 21.19006521534628 30 18.423426478320255 30.486405994882237 32.66685043349282 18.42331709330469 31 23.497068116966126 29.271284318637093 27.84854988284865 19.383097681548133 32 23.724479564326774 29.735040322962902 28.27628175538156 18.26419835732876 33 22.325627523603465 29.976489513987794 27.629415234999122 20.068467727409626 34 18.665130901048126 29.067208341263743 29.87315713595026 22.394503621737865 35 19.08320043053942 27.74292041948424 31.089591432382196 22.084287717594144 36 22.569227953267802 28.893796629920594 29.097981992309503 19.438993424502097 37 18.551297561516307 31.686067922260797 31.236167353239946 18.526467162982943 38 18.747534279440796 30.79993991116478 29.462598710861133 20.989927098533293 39 18.814003907232756 30.646290425967127 29.99304311301004 20.54666255379008 40 21.675515914425915 28.873305170337993 28.880743351396443 20.57043556383965 41 17.7686842368171 26.914511234934952 31.631995262899594 23.68480926534836 42 20.04414779228223 27.89533020783882 28.724578010840784 23.335943989038164 43 20.65506310421548 28.351101106028352 28.398501279440065 22.5953345103161 44 18.30587404825921 30.81937398226359 29.56946787106861 21.305284098408595 45 17.91566123606526 33.5135633773134 27.07173396397441 21.49904142264693 46 20.06689987551985 32.72230863638452 27.16263291190933 20.0481585761863 47 19.505937054028177 29.38774289854248 29.227676159098316 21.878643888331027 48 20.391882756764918 27.134119884518594 32.08532322907483 20.38867412964166 49 19.03474286864391 27.840309545009383 31.13356420863952 21.991383377707187 50 18.001054471550052 30.899699045360506 30.59969240933623 20.499554073753213 51 17.098008244713238 31.340010194683448 28.53738378753638 23.02459777306693 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1169.0 1 2227.5 2 3286.0 3 16248.0 4 29210.0 5 20293.0 6 11376.0 7 10993.0 8 10610.0 9 11329.0 10 12048.0 11 12388.0 12 12728.0 13 12829.0 14 12930.0 15 12491.0 16 12052.0 17 11856.0 18 11660.0 19 11698.5 20 11737.0 21 12922.0 22 14107.0 23 16846.5 24 19586.0 25 22089.0 26 30591.5 27 36591.0 28 45891.0 29 55191.0 30 63482.5 31 71774.0 32 84292.0 33 96810.0 34 108166.5 35 119523.0 36 124374.0 37 129225.0 38 139643.5 39 150062.0 40 175123.0 41 200184.0 42 222057.0 43 243930.0 44 250557.5 45 257185.0 46 247841.0 47 238497.0 48 228855.0 49 219213.0 50 201840.5 51 184468.0 52 164847.0 53 145226.0 54 128833.5 55 112441.0 56 100119.5 57 87798.0 58 77961.5 59 68125.0 60 60370.0 61 52615.0 62 44847.5 63 37080.0 64 29285.5 65 21491.0 66 16566.0 67 11641.0 68 9436.5 69 7232.0 70 5557.0 71 3882.0 72 3101.5 73 2321.0 74 1938.5 75 1196.0 76 836.0 77 580.0 78 324.0 79 263.5 80 203.0 81 116.0 82 29.0 83 24.5 84 20.0 85 21.5 86 23.0 87 15.5 88 8.0 89 8.0 90 8.0 91 5.5 92 3.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2742606.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.071412502838335 #Duplication Level Percentage of deduplicated Percentage of total 1 76.54879366524452 13.833448269192916 2 8.469474656226742 3.0611074039001673 3 2.984924250213215 1.6182539224598533 4 1.529336607586098 1.1054909076551913 5 0.9398643753322833 0.8492338411676082 6 0.6785714378964844 0.7357646620122908 7 0.5021626910786616 0.635235239381251 8 0.3927030273572695 0.56773587187893 9 0.3149732372671037 0.5122810168207383 >10 4.5061915496674745 20.246023735605025 >50 1.9289362276396722 25.253694410044275 >100 1.1985365731257045 30.02382148881035 >500 0.004712190051481255 0.5324096854129724 >1k 6.146334849758159E-4 0.3333299303041023 >5k 0.0 0.0 >10k+ 2.04877828325272E-4 0.6921696153543476 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18695 0.6816509553322643 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 4289 0.15638411058679227 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 3691 0.13458003081740505 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.03277904299779115 0.0 2 0.0 0.0 0.0 0.10085298435137967 0.0 3 0.0 0.0 0.0 0.16790599889302366 0.0 4 0.0 0.0 0.0 0.3028506464289803 0.0 5 0.0 0.0 0.0 0.5348198027715246 0.0 6 0.0 0.0 0.0 0.8865290894864227 0.0 7 0.0 0.0 0.0 1.0656652833108364 0.0 8 0.0 0.0 0.0 1.5160763157376598 0.0 9 0.0 0.0 0.0 1.6924778841729362 0.0 10 0.0 0.0 0.0 1.994307603789972 0.0 11 0.0 0.0 0.0 2.352470606423234 0.0 12 0.0 0.0 0.0 2.6472632233722235 0.0 13 0.0 0.0 0.0 2.777212621864023 0.0 14 0.0 0.0 0.0 2.837593150456172 0.0 15 0.0 0.0 0.0 2.9116468059940073 0.0 16 0.0 0.0 0.0 3.0640565943485867 0.0 17 0.0 0.0 0.0 3.251396664340412 0.0 18 0.0 0.0 0.0 3.484569055854177 0.0 19 0.0 0.0 0.0 3.6324940585705714 0.0 20 0.0 0.0 0.0 3.7807472163336624 0.0 21 0.0 0.0 0.0 3.9681237479973426 0.0 22 0.0 0.0 0.0 4.2052340000714645 0.0 23 0.0 0.0 0.0 4.444021489050924 0.0 24 0.0 0.0 0.0 4.622720142813076 0.0 25 0.0 0.0 0.0 4.779687640149551 0.0 26 0.0 0.0 0.0 4.931003578348476 0.0 27 0.0 0.0 0.0 5.076157494003878 0.0 28 0.0 0.0 0.0 5.237463930291117 0.0 29 0.0 0.0 0.0 5.437018660354422 0.0 30 0.0 0.0 0.0 5.674128912428544 0.0 31 0.0 0.0 0.0 5.8769287312869585 0.0 32 0.0 0.0 0.0 6.067586813417604 0.0 33 0.0 0.0 0.0 6.259119975672773 0.0 34 0.0 0.0 0.0 6.462503181280869 0.0 35 0.0 0.0 0.0 6.703150215524943 0.0 36 0.0 0.0 0.0 6.923451636873835 0.0 37 0.0 0.0 0.0 7.140325661068341 0.0 38 0.0 0.0 0.0 7.350891816031905 0.0 39 0.0 0.0 0.0 7.579907576954182 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCGGT 20 7.0349994E-4 45.0 17 TCGGTCG 25 3.892227E-5 44.999996 1 TAGTCCG 25 3.892227E-5 44.999996 1 CGTTTTT 9355 0.0 42.21005 1 CGACGGT 350 0.0 41.142857 28 TAGCACG 150 0.0 40.500004 1 TAGCGAG 240 0.0 40.312496 1 TTAAGCG 125 0.0 39.600002 1 AACGACG 125 0.0 39.600002 13 TCGTAAG 80 0.0 39.375 1 GTGCGTA 40 3.4605546E-7 39.375 16 TTCGCGG 225 0.0 39.0 2 TCGCGCA 375 0.0 39.0 34 TAGGGAC 3680 0.0 38.94701 5 AGGGCGA 2095 0.0 38.878284 6 TACGGGA 1100 0.0 38.659092 4 CTATGCG 105 0.0 38.57143 1 CCTAGCG 70 0.0 38.571426 1 TACGCTA 35 6.251459E-6 38.571426 42 CTATTCG 35 6.251459E-6 38.571426 42 >>END_MODULE