FastQCFastQC Report
Sat 18 Jun 2016
SRR3549286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902736
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158740.834272332052371No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20050.10537457639945846No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC207.034453E-445.00000433
CGTCGGA207.034453E-445.00000425
TCGAATA207.034453E-445.00000418
GTTGCGA207.034453E-445.00000418
CCGTAAG351.2124838E-745.01
TTAGTCG502.1827873E-1145.01
CGGTCTA900.045.031
TCTAGCG302.1662545E-644.9999961
CGTTTTT76550.042.178311
CGGGATA3650.040.0684936
ACGGGAT9300.039.677425
TATAGCG800.039.3750041
AGGGATC23250.038.709686
GAACGTA356.2504514E-638.5714269
TAAGCGA356.2504514E-638.5714261
CGTTAGG3100.038.467742
TCGATTG659.094947E-1238.076921
TAGGGCG5400.037.9166685
TACGGGA7500.037.84
TAGGGAT36100.037.770085
Produced by FastQC (version 0.11.5)