##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549286_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1902736
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.286919467545683	31.0	31.0	33.0	30.0	34.0
2	31.689322112999385	31.0	31.0	34.0	30.0	34.0
3	31.79278785916701	31.0	31.0	34.0	30.0	34.0
4	35.582217396422834	37.0	35.0	37.0	33.0	37.0
5	35.49013157894737	37.0	35.0	37.0	33.0	37.0
6	35.54151232751154	37.0	35.0	37.0	33.0	37.0
7	35.90586765583875	37.0	35.0	37.0	35.0	37.0
8	35.93615351788162	37.0	35.0	37.0	35.0	37.0
9	37.741109118658606	39.0	37.0	39.0	35.0	39.0
10	37.13585699750254	39.0	37.0	39.0	33.0	39.0
11	36.80268203260989	39.0	37.0	39.0	32.0	39.0
12	36.30831444824715	38.0	35.0	39.0	32.0	39.0
13	36.14426016010629	38.0	35.0	39.0	32.0	39.0
14	37.19424344733058	39.0	35.0	41.0	32.0	41.0
15	37.3984945888447	39.0	35.0	41.0	32.0	41.0
16	37.48315005339679	39.0	35.0	41.0	32.0	41.0
17	37.369501601903785	39.0	35.0	41.0	32.0	41.0
18	37.26812705493563	39.0	35.0	41.0	32.0	41.0
19	37.19389920619571	39.0	35.0	41.0	32.0	41.0
20	37.052728281800526	39.0	35.0	41.0	32.0	41.0
21	36.88009897326797	38.0	35.0	41.0	31.0	41.0
22	36.81437782225175	38.0	35.0	41.0	31.0	41.0
23	36.75449931046661	38.0	35.0	41.0	31.0	41.0
24	36.668665542671185	38.0	35.0	40.0	31.0	41.0
25	36.56077721764869	38.0	35.0	40.0	31.0	41.0
26	36.44436485145601	38.0	35.0	40.0	31.0	41.0
27	36.37041975344977	38.0	35.0	40.0	31.0	41.0
28	36.36539067952675	38.0	35.0	40.0	30.0	41.0
29	36.39656315957652	38.0	35.0	40.0	31.0	41.0
30	36.39828068633799	38.0	35.0	40.0	30.0	41.0
31	36.330543491057085	38.0	35.0	40.0	30.0	41.0
32	36.186397902809425	38.0	35.0	40.0	30.0	41.0
33	36.04108452249813	38.0	35.0	40.0	30.0	41.0
34	35.93598849236047	38.0	35.0	41.0	30.0	41.0
35	35.76513294540073	38.0	35.0	41.0	29.0	41.0
36	35.64545685791408	38.0	35.0	40.0	28.0	41.0
37	35.58785822100386	38.0	35.0	40.0	28.0	41.0
38	35.490485805702946	38.0	34.0	40.0	27.0	41.0
39	35.43477024663432	38.0	34.0	40.0	27.0	41.0
40	35.33671670688945	38.0	34.0	40.0	27.0	41.0
41	35.28357533572708	38.0	34.0	40.0	27.0	41.0
42	35.26442659412551	38.0	34.0	40.0	27.0	41.0
43	35.22119726541149	38.0	34.0	40.0	27.0	41.0
44	35.0956837942836	38.0	34.0	40.0	26.0	41.0
45	35.0109537003557	37.0	34.0	40.0	26.0	41.0
46	34.984710963580866	37.0	34.0	40.0	26.0	41.0
47	34.93300909847714	37.0	34.0	40.0	26.0	41.0
48	34.797597774993484	37.0	34.0	40.0	25.0	41.0
49	34.72545429318623	37.0	34.0	40.0	25.0	41.0
50	34.64836372465754	37.0	34.0	40.0	24.0	41.0
51	34.24490996123477	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	4.0
14	8.0
15	30.0
16	95.0
17	262.0
18	667.0
19	1373.0
20	2389.0
21	3987.0
22	6030.0
23	9203.0
24	13723.0
25	20048.0
26	27024.0
27	31872.0
28	35779.0
29	39869.0
30	47134.0
31	57437.0
32	70896.0
33	91750.0
34	148317.0
35	219852.0
36	140207.0
37	176073.0
38	271195.0
39	487330.0
40	175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.938324602046738	24.530833494504755	28.119980911697684	15.410860991750827
2	30.033593730291535	26.784377864296466	30.23929751683891	12.942730888573086
3	28.328207381370824	25.78308288695857	33.35113226427629	12.53757746739432
4	25.785342790592075	28.215107087898687	31.906580839380766	14.092969282128474
5	22.979803819342255	33.08257162317842	30.216645924605412	13.720978632873926
6	21.599948705443108	41.272094499709894	26.898003716753138	10.22995307809386
7	87.9853011663205	3.3478632873924705	7.156168801136889	1.5106667451501417
8	88.59537003556983	2.7657541561204497	6.724527207137511	1.9143486011722068
9	83.13391873596757	5.912853911420187	8.361906223459272	2.591321129152967
10	42.32463147804004	34.87173207423416	13.302213233995678	9.501423213730122
11	32.639998402300684	23.372659160282875	28.512100470059952	15.475241967356482
12	32.938200570126384	21.874080271777064	28.234920661615693	16.952798496480856
13	22.531922452720714	30.932247037949562	28.479515813018725	18.056314696310995
14	16.652809428107734	33.6262098367824	30.197410465771394	19.52357026933847
15	15.952607193010484	27.552955323281843	39.90805871124528	16.58637877246239
16	19.11720806249527	23.67107155170239	39.67329151285307	17.538428872949268
17	18.277575028800634	24.996688978397426	30.40568949134299	26.32004650145895
18	20.746808805845898	27.012050016397442	32.78295044609447	19.458190731662196
19	25.249009846873133	27.495564282170516	28.48025159559708	18.775174275359273
20	27.34798731931282	25.683594571185914	29.018476551660342	17.94994155784092
21	22.586422919417092	28.09806510204253	30.043789574591536	19.27172240394884
22	21.569781619730747	25.275392907896837	29.837665340856535	23.31716013151588
23	20.05674985915019	28.39332413955483	29.42215840768241	22.127767593612564
24	20.002880063235256	25.845571850219894	35.92658151209627	18.224966574448583
25	19.256849084686472	28.02112326670647	32.06840045071939	20.653627197887673
26	18.01647732528317	33.76474718510608	28.551780173392423	19.66699531621833
27	19.004002657226227	32.08022552787144	30.024028556772986	18.891743258129345
28	16.863138133719026	30.011730476534844	35.08064177058719	18.044489619158938
29	17.809512197172914	26.647206969332583	34.53574221542032	21.007538618074186
30	18.82426148451493	30.180277242875526	32.25281909839305	18.7426421742165
31	24.79886857661809	28.374456572009986	27.67262510406068	19.15404974731124
32	24.810378318379428	29.203368202420094	28.45954457160636	17.52670890759412
33	23.18897629518756	30.70531066842694	27.17213528308709	18.933577753298408
34	19.632308423238957	29.003550676499522	29.496787783486518	21.867353116775
35	20.392529494370212	28.55624742476098	30.677561154043442	20.37366192682537
36	25.09255093717678	28.31653997191413	28.216210761766213	18.374698329142877
37	21.294493823630813	31.59544992053548	29.528478990254037	17.58157726557967
38	20.921136721016474	30.82329866045526	27.82656133063126	20.429003287897007
39	21.6517162654199	30.505282918912556	28.164758537180145	19.6782422784874
40	23.439878154405026	28.152040009754376	28.599343261492926	19.808738574347675
41	18.284932854584135	27.62101521177925	30.78603652845166	23.308015405184953
42	21.83208810891264	27.942920089807522	27.28528813245768	22.93970366882216
43	21.66469757233794	27.992585413846164	28.497332262594494	21.8453847512214
44	19.734792425223468	30.958997990262443	28.84004927641039	20.4661603081037
45	18.749316773320103	33.45398415754997	26.48932905037798	21.307370018751946
46	21.320141102076168	31.655731536061754	26.646944189840315	20.377183172021763
47	20.98367824017625	28.960875286955208	28.74881223669495	21.306634236173593
48	21.84790743434717	26.389577955113058	31.24169616804433	20.52081844249544
49	20.279324089101166	27.460404386105065	30.532138983022346	21.728132541771426
50	18.89416082945821	30.14107054262914	30.50785815793678	20.456910469975867
51	18.635901144457247	30.77368589231507	27.876068986974545	22.714343976253144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1249.0
1	2134.5
2	3020.0
3	13993.5
4	24967.0
5	17399.0
6	9831.0
7	9405.0
8	8979.0
9	9408.0
10	9837.0
11	10106.0
12	10375.0
13	10024.0
14	9673.0
15	9535.5
16	9398.0
17	9121.0
18	8844.0
19	8867.5
20	8891.0
21	9787.0
22	10683.0
23	11278.5
24	11874.0
25	14453.0
26	20190.5
27	23349.0
28	30124.5
29	36900.0
30	40779.5
31	44659.0
32	50428.5
33	56198.0
34	64092.0
35	71986.0
36	74756.0
37	77526.0
38	85794.5
39	94063.0
40	111550.5
41	129038.0
42	144681.5
43	160325.0
44	164943.5
45	169562.0
46	167556.0
47	165550.0
48	160073.5
49	154597.0
50	142447.5
51	130298.0
52	115884.0
53	101470.0
54	91996.5
55	82523.0
56	72948.5
57	63374.0
58	58951.5
59	54529.0
60	51905.0
61	49281.0
62	42271.0
63	35261.0
64	28065.0
65	20869.0
66	16858.5
67	12848.0
68	11132.0
69	9416.0
70	7551.0
71	5686.0
72	4642.5
73	3599.0
74	3021.5
75	1857.5
76	1271.0
77	1003.0
78	735.0
79	570.0
80	405.0
81	301.5
82	198.0
83	127.5
84	57.0
85	56.5
86	56.0
87	30.5
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1902736.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.415535663850008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.10116130408858	15.740615083278728
2	7.4940903224099795	3.0599173649054827
3	2.627628576402889	1.6093333473871385
4	1.3770214177484192	1.124505194557126
5	0.9073276076787457	0.9261789566680569
6	0.6750478639225348	0.8268878244429767
7	0.5165218517873673	0.7381549200425992
8	0.42487960406260267	0.6939315767666029
9	0.3728257428457852	0.6850293524522549
>10	5.932551172871319	30.282596340937584
>50	2.0970875879922075	30.174584337337674
>100	0.467337177351374	12.200901422546002
>500	0.004955025837042197	0.7171676605966343
>1k	0.0013039541676426831	0.3750355605970818
>5k	0.0	0.0
>10k+	2.6079083352853667E-4	0.8451610574841109
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15874	0.834272332052371	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2005	0.10537457639945846	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0511179690719048E-4	0.0	0.0	0.04341117212266967	0.0
2	1.0511179690719048E-4	0.0	0.0	0.1435301586767686	0.0
3	1.0511179690719048E-4	0.0	0.0	0.26230648918189386	0.0
4	1.0511179690719048E-4	0.0	0.0	0.4662233751818434	0.0
5	1.0511179690719048E-4	0.0	0.0	0.781295986411147	0.0
6	1.0511179690719048E-4	0.0	0.0	1.3354453797058552	0.0
7	1.0511179690719048E-4	0.0	0.0	1.5878188040800194	0.0
8	1.0511179690719048E-4	0.0	0.0	2.23672648228656	0.0
9	1.0511179690719048E-4	0.0	0.0	2.517585203622573	0.0
10	1.0511179690719048E-4	0.0	0.0	2.919585270894123	0.0
11	1.0511179690719048E-4	0.0	0.0	3.3232145710177345	0.0
12	1.0511179690719048E-4	0.0	0.0	3.660518327292909	0.0
13	1.0511179690719048E-4	0.0	0.0	3.826384742812455	0.0
14	1.0511179690719048E-4	0.0	0.0	3.909055171079961	0.0
15	1.0511179690719048E-4	0.0	0.0	3.9993987605216907	0.0
16	1.0511179690719048E-4	0.0	0.0	4.166053514518041	0.0
17	1.0511179690719048E-4	0.0	0.0	4.370180624111805	0.0
18	1.0511179690719048E-4	0.0	0.0	4.609625297466385	0.0
19	1.0511179690719048E-4	0.0	0.0	4.758831493176142	0.0
20	1.5766769536078572E-4	0.0	0.0	4.9111910427931145	0.0
21	1.5766769536078572E-4	0.0	0.0	5.107277099923478	0.0
22	1.5766769536078572E-4	0.0	0.0	5.339101168002287	0.0
23	1.5766769536078572E-4	0.0	0.0	5.576916608504805	0.0
24	2.1022359381438096E-4	0.0	0.0	5.76380538340579	0.0
25	2.1022359381438096E-4	0.0	0.0	5.9211051874774006	0.0
26	2.1022359381438096E-4	0.0	0.0	6.071940616039219	0.0
27	2.1022359381438096E-4	0.0	0.0	6.218729240420111	0.0
28	2.1022359381438096E-4	0.0	0.0	6.383912429259761	0.0
29	2.1022359381438096E-4	0.0	0.0	6.558765903414872	0.0
30	2.1022359381438096E-4	0.0	0.0	6.7788174502400755	0.0
31	2.1022359381438096E-4	0.0	0.0	6.969542805728173	0.0
32	2.1022359381438096E-4	0.0	0.0	7.159742602231733	0.0
33	2.1022359381438096E-4	0.0	0.0	7.341375787287359	0.0
34	2.1022359381438096E-4	0.0	0.0	7.538460406488341	0.0
35	2.1022359381438096E-4	0.0	0.0	7.753992146046535	0.0
36	2.1022359381438096E-4	0.0	0.0	7.96836765583875	0.0
37	2.1022359381438096E-4	0.0	0.0	8.18516073695983	0.0
38	2.1022359381438096E-4	0.0	0.0	8.390076185030399	0.0
39	2.1022359381438096E-4	0.0	0.0	8.594150738725709	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTATC	20	7.034453E-4	45.000004	33
CGTCGGA	20	7.034453E-4	45.000004	25
TCGAATA	20	7.034453E-4	45.000004	18
GTTGCGA	20	7.034453E-4	45.000004	18
CCGTAAG	35	1.2124838E-7	45.0	1
TTAGTCG	50	2.1827873E-11	45.0	1
CGGTCTA	90	0.0	45.0	31
TCTAGCG	30	2.1662545E-6	44.999996	1
CGTTTTT	7655	0.0	42.17831	1
CGGGATA	365	0.0	40.068493	6
ACGGGAT	930	0.0	39.67742	5
TATAGCG	80	0.0	39.375004	1
AGGGATC	2325	0.0	38.70968	6
GAACGTA	35	6.2504514E-6	38.571426	9
TAAGCGA	35	6.2504514E-6	38.571426	1
CGTTAGG	310	0.0	38.46774	2
TCGATTG	65	9.094947E-12	38.07692	1
TAGGGCG	540	0.0	37.916668	5
TACGGGA	750	0.0	37.8	4
TAGGGAT	3610	0.0	37.77008	5
>>END_MODULE