FastQCFastQC Report
Sat 18 Jun 2016
SRR3549285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132116
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66260.5852757137961128No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26740.2361948775567168No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAC453.8562575E-1045.00000416
CGGAATT207.0332404E-445.00000432
CTATGCG406.8157533E-945.0000041
CGTATTG406.8157533E-945.00000436
TAGACGT207.0332404E-445.00000411
TAGGTTG207.0332404E-445.00000411
ATTCGAA406.8157533E-945.00000423
CAATTCG207.0332404E-445.0000041
TAAGTCG207.0332404E-445.00000434
CGCGAAA207.0332404E-445.00000424
TATTGCG406.8157533E-945.0000041
GTAGATC207.0332404E-445.00000413
ACGTGCG406.8157533E-945.0000041
GCGATAC406.8157533E-945.0000049
CTCGCAG207.0332404E-445.00000425
CAGTCGT207.0332404E-445.00000444
ATCTCGC253.890768E-545.031
ACGCATG351.2119017E-745.01
TCCGATT253.890768E-545.012
ATAGCCG253.890768E-545.01