##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549285_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1132116 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.332694706196186 31.0 31.0 33.0 30.0 34.0 2 31.734679131820414 31.0 31.0 34.0 30.0 34.0 3 31.871611212985243 31.0 31.0 34.0 30.0 34.0 4 35.63306940278205 37.0 35.0 37.0 33.0 37.0 5 35.53618092138968 37.0 35.0 37.0 33.0 37.0 6 35.57982309233329 37.0 35.0 37.0 33.0 37.0 7 35.89718721403107 37.0 35.0 37.0 35.0 37.0 8 35.92474976062524 37.0 35.0 37.0 35.0 37.0 9 37.77942277999781 39.0 38.0 39.0 35.0 39.0 10 37.18955831381236 39.0 37.0 39.0 33.0 39.0 11 36.823435054358384 39.0 35.0 39.0 33.0 39.0 12 35.75287956357829 37.0 35.0 39.0 32.0 39.0 13 35.32733924792159 37.0 35.0 39.0 30.0 39.0 14 36.26604517558271 38.0 35.0 41.0 31.0 41.0 15 36.622564295531554 38.0 35.0 41.0 32.0 41.0 16 36.788201915704754 38.0 35.0 41.0 32.0 41.0 17 36.71674192397246 38.0 35.0 41.0 32.0 41.0 18 36.669776771991565 38.0 35.0 40.0 32.0 41.0 19 36.595614760324914 37.0 35.0 40.0 32.0 41.0 20 36.38897780792781 37.0 35.0 40.0 31.0 41.0 21 36.15579587250777 37.0 35.0 40.0 31.0 41.0 22 36.0422368379212 37.0 35.0 40.0 31.0 41.0 23 36.07417879439916 36.0 35.0 40.0 31.0 41.0 24 35.96335622851369 36.0 35.0 40.0 31.0 41.0 25 35.87237438566366 36.0 35.0 40.0 31.0 41.0 26 35.7527850502952 36.0 34.0 40.0 30.0 41.0 27 35.70514063929845 36.0 34.0 40.0 30.0 41.0 28 35.75495002278918 36.0 35.0 40.0 30.0 41.0 29 35.825324436718496 36.0 35.0 40.0 30.0 41.0 30 35.857549049744016 36.0 35.0 40.0 30.0 41.0 31 35.70076211271636 36.0 35.0 40.0 30.0 41.0 32 35.51666701998735 36.0 34.0 40.0 30.0 41.0 33 35.38781803278109 36.0 34.0 40.0 29.0 41.0 34 35.325616809584886 36.0 34.0 40.0 29.0 41.0 35 35.18086220846627 36.0 34.0 40.0 29.0 41.0 36 34.99556582541012 36.0 34.0 40.0 27.0 41.0 37 34.947556610806664 36.0 34.0 40.0 27.0 41.0 38 34.90683551862177 35.0 34.0 40.0 27.0 41.0 39 34.89120902805013 36.0 34.0 40.0 27.0 41.0 40 34.768566118666286 36.0 34.0 40.0 26.0 41.0 41 34.7661476385812 36.0 34.0 40.0 26.0 41.0 42 34.70550544290514 36.0 34.0 40.0 26.0 41.0 43 34.65095272922563 35.0 34.0 40.0 27.0 41.0 44 34.52202424486537 35.0 34.0 40.0 26.0 41.0 45 34.43371438969152 35.0 34.0 40.0 26.0 41.0 46 34.440149242657114 35.0 34.0 40.0 26.0 41.0 47 34.39147048535663 35.0 33.0 40.0 26.0 41.0 48 34.30949213684817 35.0 34.0 40.0 26.0 41.0 49 34.296616247805 35.0 34.0 40.0 26.0 41.0 50 34.17581237258373 35.0 34.0 40.0 24.0 41.0 51 33.71180515070894 35.0 33.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 2.0 13 2.0 14 6.0 15 27.0 16 78.0 17 207.0 18 468.0 19 995.0 20 1712.0 21 2685.0 22 4254.0 23 6263.0 24 8977.0 25 12904.0 26 17638.0 27 20412.0 28 22624.0 29 25482.0 30 30231.0 31 36839.0 32 46021.0 33 60704.0 34 114617.0 35 197577.0 36 70116.0 37 84855.0 38 130831.0 39 235493.0 40 95.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.53315031321879 24.49987457115702 28.907903430390526 14.059071685233668 2 29.513406753371562 26.63198824148762 30.031198216437183 13.823406788703632 3 29.305654190913298 25.123397249045148 32.182567864070464 13.388380695971083 4 25.8627207812627 27.975755134632845 31.183641958951203 14.977882125153252 5 23.210254072904192 32.849107335290725 30.343798692006825 13.596839899798255 6 22.523751983012342 41.24877662713008 26.1724063611856 10.055065028671974 7 88.74638287949291 3.7323913803885818 6.177635507315505 1.343590232802999 8 89.6531804161411 2.905179327913394 5.847810648378788 1.5938296075667158 9 85.39063134873105 5.1109603609524115 7.2634783008101635 2.2349299895063757 10 51.672620120199696 27.41326860498394 12.078709248875557 8.835402025940803 11 48.00258984061704 18.54871762257578 21.24102123810634 12.20767129870084 12 44.48810899236474 20.156326736836156 22.477643633691248 12.87792063710786 13 21.06851241392225 41.54008953146144 23.635387186472055 13.756010868144253 14 15.20444901405863 43.665048457931874 25.695246776832054 15.43525575117744 15 13.731896731430348 24.111486808772245 48.57876754678849 13.577848913008914 16 15.487193891792007 19.899815919923398 47.45635606245296 17.15663412583163 17 16.036519225945046 20.83116924414106 27.368838528913997 35.763473000999895 18 21.504333478194816 24.489716601479 34.564214267795876 19.441735652530305 19 28.911613297577283 25.41444516286317 26.186097537708147 19.4878440018514 20 32.413286271018166 22.70191393814768 26.42123245321151 18.463567337622646 21 21.902879210257606 29.412887018644735 28.28950390242696 20.3947298686707 22 23.02378908168421 23.415445060400174 27.01454621257892 26.5462196453367 23 19.011214398524533 30.061053814273446 25.24193633867908 25.685795448522942 24 19.37654798624876 23.807719350313924 40.415646453190305 16.400086210247007 25 16.405827671369366 25.663359585060185 36.57416731147692 21.356645432093533 26 16.035282603549458 37.41913372834586 26.770048298937567 19.775535369167116 27 16.52904826007229 36.96829653498405 29.829010454759054 16.67364475018461 28 13.84345773754633 29.32544014924266 40.569164290585064 16.26193782262595 29 14.640637531843026 24.475495443929773 39.2037565055171 21.680110518710098 30 17.369068187358895 32.474145758915164 31.962272417314125 18.194513636411816 31 29.76134954368634 27.654056651438548 25.190174858406735 17.39441894646838 32 29.152401344031887 27.58772069293253 26.79354412445368 16.46633383858191 33 27.432171261602168 29.47604309099068 24.07217988262687 19.019605764780287 34 18.538647983068874 29.19214992103283 26.89432884969385 25.374873246204455 35 19.900434231121192 27.524387960244358 30.917326493044882 21.657851315589568 36 30.38151567507217 25.384854555540244 27.28139165951192 16.952238109875665 37 19.192114588964383 33.88884177946429 30.268364725876147 16.650678905695177 38 19.594281858042812 33.60335866642641 24.89559373774419 21.90676573778659 39 19.231156524596418 33.26540743174728 28.98651728268128 18.516918760975024 40 24.481766886078812 27.908270883902357 26.04653586734928 21.56342636266955 41 16.66596002529776 24.91900123308919 30.137724402799716 28.277314338813337 42 21.875143536528057 25.249532733394815 25.996629320670323 26.878694409406812 43 23.53301251815185 25.41153026721643 25.84947125559572 25.205985959036 44 18.062813351282024 32.002727635683975 28.83679764264439 21.097661370389606 45 16.143398732992026 40.46873288602934 22.7555303520134 20.63233802896523 46 22.473138794964473 34.618272332517165 25.677669072780528 17.230919799737837 47 20.359397800225416 28.861353430213864 27.392246024259 23.387002745301718 48 23.077582155892152 24.40933614576598 32.83073465969918 19.682347038642682 49 21.028233855894626 25.00503481975345 32.415759515809334 21.550971808542588 50 18.79409883792827 33.37882337145663 29.014694607266396 18.8123831833487 51 17.953813920128326 35.043228785742805 24.23135085097287 22.771606443156 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 574.0 1 1027.0 2 1480.0 3 5938.5 4 10397.0 5 7420.0 6 4443.0 7 4378.0 8 4313.0 9 4712.0 10 5111.0 11 5227.5 12 5344.0 13 5150.0 14 4956.0 15 4587.0 16 4218.0 17 4257.0 18 4296.0 19 4322.0 20 4348.0 21 4248.5 22 4149.0 23 4618.5 24 5088.0 25 5911.5 26 9102.5 27 11470.0 28 13952.5 29 16435.0 30 19081.5 31 21728.0 32 24867.5 33 28007.0 34 30520.5 35 33034.0 36 34162.5 37 35291.0 38 40466.5 39 45642.0 40 69072.0 41 92502.0 42 106774.5 43 121047.0 44 124193.0 45 127339.0 46 121053.5 47 114768.0 48 103690.5 49 92613.0 50 84643.5 51 76674.0 52 68245.5 53 59817.0 54 52897.5 55 45978.0 56 40935.0 57 35892.0 58 32698.5 59 29505.0 60 26253.0 61 23001.0 62 20395.5 63 17790.0 64 15582.0 65 13374.0 66 11402.0 67 9430.0 68 7658.5 69 5887.0 70 4870.5 71 3854.0 72 3055.5 73 2257.0 74 1939.0 75 1203.0 76 785.0 77 644.5 78 504.0 79 356.0 80 208.0 81 176.5 82 145.0 83 96.5 84 48.0 85 30.0 86 12.0 87 8.5 88 5.0 89 3.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1132116.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.62630684335939 #Duplication Level Percentage of deduplicated Percentage of total 1 80.44738223774013 13.375428618296874 2 7.037998750227057 2.340318535689099 3 2.078239340757319 1.036603349201164 4 0.9635793496016729 0.6408306373760834 5 0.5820384281885917 0.4838574750845064 6 0.389319617252325 0.3883768449945834 7 0.30893265252312596 0.35954863523476926 8 0.25930094107488344 0.3448973608866292 9 0.231422002931918 0.3462923907945797 >10 4.164812413573478 18.35710272806579 >50 1.9532865175648475 23.363116924178822 >100 1.5777784659207772 37.53695376780535 >500 0.00429766010465312 0.4954456674749295 >1k 0.00107441502616328 0.3394075241400274 >5k 5.3720751308164E-4 0.5918195407767951 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6626 0.5852757137961128 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 2674 0.2361948775567168 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.02800066424288677 0.0 2 0.0 0.0 0.0 0.12251394733401877 0.0 3 0.0 0.0 0.0 0.18487504813994327 0.0 4 0.0 0.0 0.0 0.38167466938016953 0.0 5 0.0 0.0 0.0 0.6065632850343957 0.0 6 0.0 0.0 0.0 0.8917814075589428 0.0 7 0.0 0.0 0.0 1.0110271385617728 0.0 8 0.0 0.0 0.0 1.3417352992096216 0.0 9 0.0 0.0 0.0 1.4353652805896215 0.0 10 0.0 0.0 0.0 1.6270417519052818 0.0 11 0.0 0.0 0.0 1.8904423221648665 0.0 12 0.0 0.0 0.0 2.0931600648696778 0.0 13 0.0 0.0 0.0 2.177162057598338 0.0 14 0.0 0.0 0.0 2.2093142398835455 0.0 15 0.0 0.0 0.0 2.2570125322846777 0.0 16 0.0 0.0 0.0 2.3920693639167716 0.0 17 0.0 0.0 0.0 2.543643937547036 0.0 18 0.0 0.0 0.0 2.7383236346805453 0.0 19 0.0 0.0 0.0 2.8280670885315637 0.0 20 0.0 0.0 0.0 2.9175455518692432 0.0 21 0.0 0.0 0.0 3.063202004034922 0.0 22 0.0 0.0 0.0 3.2093884372272807 0.0 23 0.0 0.0 0.0 3.3672344530065823 0.0 24 0.0 0.0 0.0 3.489836730511714 0.0 25 0.0 0.0 0.0 3.589914814382978 0.0 26 0.0 0.0 0.0 3.6939677559543367 0.0 27 0.0 0.0 0.0 3.7901593122966197 0.0 28 0.0 0.0 0.0 3.892798971130167 0.0 29 0.0 0.0 0.0 4.003211685021676 0.0 30 0.0 0.0 0.0 4.152224683689657 0.0 31 0.0 0.0 0.0 4.293906278155242 0.0 32 0.0 0.0 0.0 4.418010168569299 0.0 33 0.0 0.0 0.0 4.540082465047751 0.0 34 0.0 0.0 0.0 4.67390267428426 0.0 35 0.0 0.0 0.0 4.857982750884185 0.0 36 0.0 0.0 0.0 4.998957703980865 0.0 37 0.0 0.0 0.0 5.1516805698356 0.0 38 0.0 0.0 0.0 5.279141006751958 0.0 39 0.0 0.0 0.0 5.42046928053309 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCATAAC 45 3.8562575E-10 45.000004 16 CGGAATT 20 7.0332404E-4 45.000004 32 CTATGCG 40 6.8157533E-9 45.000004 1 CGTATTG 40 6.8157533E-9 45.000004 36 TAGACGT 20 7.0332404E-4 45.000004 11 TAGGTTG 20 7.0332404E-4 45.000004 11 ATTCGAA 40 6.8157533E-9 45.000004 23 CAATTCG 20 7.0332404E-4 45.000004 1 TAAGTCG 20 7.0332404E-4 45.000004 34 CGCGAAA 20 7.0332404E-4 45.000004 24 TATTGCG 40 6.8157533E-9 45.000004 1 GTAGATC 20 7.0332404E-4 45.000004 13 ACGTGCG 40 6.8157533E-9 45.000004 1 GCGATAC 40 6.8157533E-9 45.000004 9 CTCGCAG 20 7.0332404E-4 45.000004 25 CAGTCGT 20 7.0332404E-4 45.000004 44 ATCTCGC 25 3.890768E-5 45.0 31 ACGCATG 35 1.2119017E-7 45.0 1 TCCGATT 25 3.890768E-5 45.0 12 ATAGCCG 25 3.890768E-5 45.0 1 >>END_MODULE