##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549281_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1549377
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.434979995185163	31.0	31.0	33.0	30.0	34.0
2	31.855809141351653	31.0	31.0	34.0	30.0	34.0
3	32.011086391498004	33.0	31.0	34.0	30.0	34.0
4	35.728288208744544	37.0	35.0	37.0	35.0	37.0
5	35.6288889017973	37.0	35.0	37.0	33.0	37.0
6	35.68356507163847	37.0	35.0	37.0	33.0	37.0
7	35.97306143049755	37.0	35.0	37.0	35.0	37.0
8	35.89579101793818	37.0	35.0	37.0	35.0	37.0
9	37.521207556327475	39.0	37.0	39.0	35.0	39.0
10	37.1777598350821	39.0	37.0	39.0	33.0	39.0
11	36.92684608071502	39.0	37.0	39.0	33.0	39.0
12	36.33299706914457	38.0	35.0	39.0	32.0	39.0
13	36.09578043303857	38.0	35.0	39.0	32.0	39.0
14	37.13018587470964	39.0	35.0	41.0	32.0	41.0
15	37.36794143710666	39.0	35.0	41.0	32.0	41.0
16	37.447360455202315	39.0	35.0	41.0	33.0	41.0
17	37.34958567217662	39.0	35.0	41.0	32.0	41.0
18	37.29427505377968	39.0	35.0	41.0	32.0	41.0
19	37.31048673111838	39.0	35.0	41.0	32.0	41.0
20	37.19385727295552	39.0	35.0	41.0	32.0	41.0
21	37.07832438457522	39.0	35.0	41.0	32.0	41.0
22	37.00361951932938	39.0	35.0	41.0	32.0	41.0
23	36.93959894848058	39.0	35.0	41.0	32.0	41.0
24	36.886779654015776	38.0	35.0	41.0	32.0	41.0
25	36.84197906642476	38.0	35.0	41.0	32.0	41.0
26	36.831212158177124	38.0	35.0	41.0	32.0	41.0
27	36.799696910435614	38.0	35.0	41.0	31.0	41.0
28	36.77847483214221	38.0	35.0	41.0	31.0	41.0
29	36.755492046157904	38.0	35.0	41.0	31.0	41.0
30	36.69183807427114	38.0	35.0	41.0	31.0	41.0
31	36.568862194288414	38.0	35.0	41.0	31.0	41.0
32	36.44938965790766	38.0	35.0	41.0	30.0	41.0
33	36.3721528072251	38.0	35.0	41.0	30.0	41.0
34	36.28444916892403	38.0	35.0	41.0	30.0	41.0
35	36.2141925431964	38.0	35.0	41.0	30.0	41.0
36	36.193879217259585	38.0	35.0	41.0	30.0	41.0
37	36.17133273567376	38.0	35.0	41.0	30.0	41.0
38	36.07914600513626	38.0	35.0	41.0	30.0	41.0
39	36.06141371660997	38.0	35.0	41.0	30.0	41.0
40	35.9607726202209	38.0	35.0	40.0	29.0	41.0
41	35.94035215444659	38.0	35.0	40.0	30.0	41.0
42	35.913116045997846	38.0	35.0	40.0	30.0	41.0
43	35.89511138993286	38.0	35.0	40.0	30.0	41.0
44	35.75384428709088	38.0	35.0	40.0	29.0	41.0
45	35.68047157018595	38.0	35.0	40.0	29.0	41.0
46	35.644195053882946	38.0	35.0	40.0	29.0	41.0
47	35.59382254932144	38.0	34.0	40.0	29.0	41.0
48	35.52615406063211	38.0	34.0	40.0	29.0	41.0
49	35.45563281241428	37.0	34.0	40.0	28.0	41.0
50	35.38448615153058	37.0	34.0	40.0	28.0	41.0
51	34.952538988251405	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	5.0
11	7.0
12	8.0
13	8.0
14	19.0
15	38.0
16	92.0
17	195.0
18	461.0
19	854.0
20	1596.0
21	2596.0
22	3846.0
23	5937.0
24	8833.0
25	12458.0
26	16718.0
27	20762.0
28	24079.0
29	28157.0
30	34347.0
31	42530.0
32	53901.0
33	70968.0
34	126514.0
35	189475.0
36	102254.0
37	136875.0
38	219739.0
39	445923.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.16148748819687	23.329312362323694	29.074137540443672	12.43506260903576
2	31.34647022642004	25.094086203680575	29.12990188959821	14.429541680301178
3	30.589004483737657	24.942799589770598	30.30295402603756	14.16524190045418
4	28.82552148379639	27.573211684438327	27.75057329494371	15.850693536821575
5	24.94951196513179	31.998474225446742	27.56804831877587	15.483965490645597
6	22.24765179810982	41.02778084352614	26.194399426350074	10.530167932013965
7	85.55529093306535	5.139678722480068	6.5308830581582145	2.774147286296363
8	85.14822409265143	5.151296295220595	6.983452058472535	2.717027553655437
9	79.52105910956469	8.161925728857469	8.157085073548917	4.159930088028931
10	42.69032004476638	34.82580417806641	13.086937523920906	9.396938253246304
11	37.75498151837803	23.66564109316196	23.802986619783304	14.7763907686767
12	37.07541805512796	21.84129492047449	24.857733140481624	16.22555388391592
13	26.014391590942683	31.14593801250438	24.648681373222914	18.190989023330022
14	17.179614774196338	35.4203011920275	27.40830669359362	19.991777340182537
15	18.381969010770135	30.014128259293898	36.5714735664722	15.032429163463767
16	21.875050423492798	25.978828909942514	35.73481470294189	16.411305963622798
17	21.177479722494912	25.9668240847773	27.75154142600542	25.104154766722363
18	22.350080064438803	28.92156008511808	29.73627464458295	18.992085205860164
19	26.303669152181815	30.40415599302171	24.720387613860282	18.571787240936196
20	28.244578304699242	29.20670695382725	25.421379044609544	17.127335696863966
21	25.834512839676847	29.319074699056458	27.754703987473672	17.091708473793016
22	23.6454394250076	25.112028899357615	27.864231881588537	23.378299794046253
23	21.29675346929766	28.270201506799186	29.84948143673231	20.583563587170843
24	22.171040360093123	26.98768601831575	33.04386214588186	17.797411475709268
25	21.144627808467533	28.72018882428228	29.17604947020641	20.95913389704378
26	18.698676952091066	33.6402308798956	26.696278568740855	20.964813599272482
27	19.278071121489475	33.64068267439106	28.703214259666947	18.37803194445251
28	17.2690700842984	31.589922917404866	32.77930419775174	18.361702800544993
29	18.59973395758424	28.466732112326437	32.556956763912204	20.376577166177114
30	20.671921682069634	30.232861337169716	30.5487302315705	18.54648674919016
31	26.50529858130074	28.975839966644656	25.640241206626925	18.87862024542768
32	26.90481399943332	29.280736709012718	26.236739024782217	17.57771026677174
33	25.70497690361997	28.972741947247183	26.546540964529612	18.775740184603233
34	19.942402656035295	30.352715962609487	28.078447014509706	21.626434366845512
35	19.59587627801368	29.078461859185982	29.506763040886756	21.81889882191358
36	24.24219541144602	31.062291488772587	26.8393037975909	17.856209302190493
37	21.568217419001314	32.10929296097722	28.171452138504705	18.151037481516767
38	19.54017647092993	32.873858331445476	26.39577068718588	21.19019451043871
39	21.042522252492454	30.554151765516075	27.7332114779037	20.67011450408777
40	23.0522977945329	28.89684047200907	26.899779717912427	21.1510820155456
41	18.845832873471082	27.510993128205723	27.957688800079	25.685485198244194
42	19.972543803089888	27.797495380401283	27.070106242702714	25.15985457380612
43	22.036276516303005	26.172132411930733	26.80367657451995	24.987914497246315
44	19.225921128298666	29.73253120447767	27.0614576052181	23.98009006200557
45	19.42516250079871	33.7573101962918	25.01127872686893	21.806248576040563
46	21.983352018262824	32.391019099935	25.91848207376255	19.707146808039617
47	21.119327316721495	29.17888932132076	27.049646406265225	22.652136955692516
48	20.66178857695706	26.502716898469515	30.964510251539814	21.870984273033613
49	21.181610415024878	26.64677480045205	30.401122515695018	21.77049226882805
50	19.311052119658417	28.894387873319406	28.86185866964593	22.932701337376248
51	18.497692943679944	30.023228691273978	28.137503009274052	23.34157535577203
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1256.0
1	2294.5
2	3333.0
3	6741.0
4	10149.0
5	7498.5
6	4848.0
7	4799.5
8	4751.0
9	5015.0
10	5279.0
11	5360.0
12	5441.0
13	5251.0
14	5061.0
15	4674.0
16	4287.0
17	3976.0
18	3665.0
19	4255.0
20	4845.0
21	6396.0
22	7947.0
23	8255.5
24	8564.0
25	11926.0
26	17827.5
27	20367.0
28	21947.0
29	23527.0
30	28321.5
31	33116.0
32	37444.5
33	41773.0
34	47644.5
35	53516.0
36	56841.5
37	60167.0
38	66055.0
39	71943.0
40	87448.5
41	102954.0
42	114728.0
43	126502.0
44	134501.0
45	142500.0
46	137186.5
47	131873.0
48	132710.5
49	133548.0
50	125096.5
51	116645.0
52	101774.0
53	86903.0
54	81304.0
55	75705.0
56	67870.0
57	60035.0
58	59623.0
59	59211.0
60	56065.5
61	52920.0
62	45339.0
63	37758.0
64	27170.0
65	16582.0
66	12465.5
67	8349.0
68	6264.5
69	4180.0
70	3353.0
71	2526.0
72	1812.0
73	1098.0
74	720.5
75	335.0
76	327.0
77	183.5
78	40.0
79	123.0
80	206.0
81	110.5
82	15.0
83	15.5
84	16.0
85	11.0
86	6.0
87	4.5
88	3.0
89	3.5
90	4.0
91	4.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1549377.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.206449123393062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.0525560251631	11.56489323900643
2	10.389090028546219	3.1596233791487647
3	3.8842783139231627	1.7719824168531466
4	1.955642067608285	1.189534864186104
5	1.0870282684495987	0.8264920029934437
6	0.7209620570655204	0.6577963704398184
7	0.47190649950916924	0.5023215523049287
8	0.32780715691389983	0.39878262831162753
9	0.27670038139832487	0.37868672449413737
>10	2.1898675678977404	7.382870177189635
>50	0.8269186569358499	9.270339920801797
>100	1.7940221718717544	59.68766260568498
>500	0.0193506705978497	1.9652309795203264
>1k	0.003440119217395502	0.8944043833725636
>5k	4.3001490217443774E-4	0.3493787556923239
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5343	0.34484828418131935	No Hit
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG	3231	0.20853543069246544	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2284	0.14741408966313557	No Hit
AGGCAGGAGACTCAGCCCGAGGAAATCGCAGATAAGTTTTTAATTAAAAAG	1674	0.10804342648690408	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2908414156141468E-4	0.0	0.0	0.07002814679706747	0.0
2	1.2908414156141468E-4	0.0	0.0	0.22041117171611557	0.0
3	1.2908414156141468E-4	0.0	0.0	0.31109278116300937	0.0
4	1.2908414156141468E-4	0.0	0.0	0.46457382547953147	0.0
5	1.2908414156141468E-4	0.0	0.0	0.8019352294502887	0.0
6	1.2908414156141468E-4	0.0	0.0	1.077529871683909	0.0
7	1.2908414156141468E-4	0.0	0.0	1.2500508268807398	0.0
8	1.2908414156141468E-4	0.0	0.0	1.6400140185377736	0.0
9	1.2908414156141468E-4	0.0	0.0	1.756835166650854	0.0
10	1.2908414156141468E-4	0.0	0.0	2.016423375330859	0.0
11	1.2908414156141468E-4	0.0	0.0	2.4651198513983363	0.0
12	1.2908414156141468E-4	0.0	0.0	2.800609535316453	0.0
13	1.2908414156141468E-4	0.0	0.0	2.9540905796329753	0.0
14	1.2908414156141468E-4	0.0	0.0	3.0196653235461737	0.0
15	1.2908414156141468E-4	0.0	0.0	3.129515928014938	0.0
16	1.2908414156141468E-4	0.0	0.0	3.369999683743853	0.0
17	1.2908414156141468E-4	0.0	0.0	3.6454006997651316	0.0
18	1.2908414156141468E-4	0.0	0.0	3.9393898321712535	0.0
19	1.2908414156141468E-4	0.0	0.0	4.132822418301033	0.0
20	1.9362621234212201E-4	0.0	0.0	4.329933902465314	0.0
21	1.9362621234212201E-4	0.0	0.0	4.582874277854906	0.0
22	1.9362621234212201E-4	0.0	0.0	4.849497572250008	0.0
23	1.9362621234212201E-4	0.0	0.0	5.14535842470877	0.0
24	1.9362621234212201E-4	0.0	0.0	5.364220586726149	0.0
25	1.9362621234212201E-4	0.0	0.0	5.5684316986763065	0.0
26	1.9362621234212201E-4	0.0	0.0	5.7477941133758925	0.0
27	1.9362621234212201E-4	0.0	0.0	5.9301254633313905	0.0
28	1.9362621234212201E-4	0.0	0.0	6.137305510537461	0.0
29	1.9362621234212201E-4	0.0	0.0	6.363783636906963	0.0
30	1.9362621234212201E-4	0.0	0.0	6.61349690875752	0.0
31	1.9362621234212201E-4	0.0	0.0	6.85985399292748	0.0
32	1.9362621234212201E-4	0.0	0.0	7.087816586924938	0.0
33	1.9362621234212201E-4	0.0	0.0	7.324040565982327	0.0
34	1.9362621234212201E-4	0.0	0.0	7.561684470596892	0.0
35	1.9362621234212201E-4	0.0	0.0	7.835471934848652	0.0
36	1.9362621234212201E-4	0.0	0.0	8.0989326677755	0.0
37	1.9362621234212201E-4	0.0	0.0	8.352841174226802	0.0
38	1.9362621234212201E-4	0.0	0.0	8.629791199946817	0.0
39	1.9362621234212201E-4	0.0	0.0	8.921263191592491	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	55	1.8189894E-12	45.000004	6
CTCGCGC	220	0.0	45.000004	33
GCCCCCG	20	7.0340483E-4	45.0	12
CTAGACG	100	0.0	45.0	1
TTAGGCG	35	1.2123019E-7	45.0	1
AGCCGAT	35	1.2123019E-7	45.0	38
CGAACGC	20	7.0340483E-4	45.0	25
CGAACCA	20	7.0340483E-4	45.0	15
CGAACAC	35	1.2123019E-7	45.0	28
TCCGCAA	35	1.2123019E-7	45.0	22
CTCGTAG	25	3.8914368E-5	45.0	1
ACGCATA	25	3.8914368E-5	45.0	35
TCCGATC	20	7.0340483E-4	45.0	17
CGTGCGT	35	1.2123019E-7	45.0	30
ACGTTAG	50	2.1827873E-11	45.0	1
CCGATAT	25	3.8914368E-5	45.0	11
AACCCGC	40	6.8175723E-9	45.0	19
GCGCGAT	25	3.8914368E-5	45.0	40
CATGACG	20	7.0340483E-4	45.0	30
CGTTGCG	20	7.0340483E-4	45.0	15
>>END_MODULE