FastQCFastQC Report
Sat 18 Jun 2016
SRR3549280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434984
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG66350.46237449337414216No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG57520.40084070623783963No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51880.36153713212133376No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG31500.21951464267197404No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC29790.2075981334983526No Hit
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG26280.1831379304577612No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22480.15665679896082466No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20710.1443221666583042No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA20670.14404341790570488No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT20590.1434859204005062No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA19650.1369353247144219No Hit
GCCGAAGGGAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACT16570.11547167076427334No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA15490.10794545444409137No Hit
TAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14960.10425203347215022No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG14730.10264922814470405No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCC253.8912913E-545.00000428
AGCCGTA253.8912913E-545.00000442
ATCTCGC253.8912913E-545.00000412
AGTCCGC253.8912913E-545.00000425
CGAGTCG253.8912913E-545.00000427
CCGATTC253.8912913E-545.00000437
ACGTTAT502.1827873E-1145.00000438
TCCGAGT253.8912913E-545.00000412
CACGCTT253.8912913E-545.00000426
GCACGTA502.1827873E-1145.0000049
TCCTCGA253.8912913E-545.00000421
GTTACGT253.8912913E-545.00000425
CCACTAG502.1827873E-1145.00000445
CCGTAAA253.8912913E-545.00000430
AAGTTCG253.8912913E-545.00000415
CGTAGAT253.8912913E-545.00000433
CGCTAAT253.8912913E-545.00000440
TATTCCG253.8912913E-545.00000435
TCGCTAA253.8912913E-545.00000439
TCCACGA253.8912913E-545.00000444