##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549280_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1434984
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.432957440640454	31.0	31.0	33.0	30.0	34.0
2	31.852835292937066	31.0	31.0	34.0	30.0	34.0
3	32.003639761837064	33.0	31.0	34.0	30.0	34.0
4	35.71385604299421	37.0	35.0	37.0	33.0	37.0
5	35.6129357539875	37.0	35.0	37.0	33.0	37.0
6	35.65489998494757	37.0	35.0	37.0	33.0	37.0
7	35.96433688459244	37.0	35.0	37.0	35.0	37.0
8	35.885540884079546	37.0	35.0	37.0	35.0	37.0
9	37.45489427059814	39.0	37.0	39.0	35.0	39.0
10	37.16733566367291	39.0	37.0	39.0	33.0	39.0
11	36.956963980086186	39.0	37.0	39.0	33.0	39.0
12	36.395223918872965	38.0	35.0	39.0	32.0	39.0
13	36.14579186945638	38.0	35.0	39.0	32.0	39.0
14	37.2409943246754	39.0	35.0	41.0	32.0	41.0
15	37.462860213075544	39.0	35.0	41.0	32.0	41.0
16	37.56123901033043	39.0	35.0	41.0	33.0	41.0
17	37.50314080156991	39.0	35.0	41.0	33.0	41.0
18	37.43692961036499	39.0	35.0	41.0	32.0	41.0
19	37.40873417403957	39.0	35.0	41.0	32.0	41.0
20	37.296354523813505	39.0	35.0	41.0	32.0	41.0
21	37.161173922496694	39.0	35.0	41.0	32.0	41.0
22	37.05569469764123	39.0	35.0	41.0	32.0	41.0
23	37.00931648018375	39.0	35.0	41.0	32.0	41.0
24	36.9511102562816	39.0	35.0	41.0	32.0	41.0
25	36.89433401348029	38.0	35.0	41.0	31.0	41.0
26	36.907734859761504	39.0	35.0	41.0	32.0	41.0
27	36.888794578894256	39.0	35.0	41.0	31.0	41.0
28	36.840692997273834	39.0	35.0	41.0	31.0	41.0
29	36.83624834841364	39.0	35.0	41.0	31.0	41.0
30	36.76257574997352	38.0	35.0	41.0	31.0	41.0
31	36.65307069625863	38.0	35.0	41.0	31.0	41.0
32	36.51192765912373	38.0	35.0	41.0	30.0	41.0
33	36.40894881057907	38.0	35.0	41.0	30.0	41.0
34	36.37759933211799	38.0	35.0	41.0	30.0	41.0
35	36.28434533067964	38.0	35.0	41.0	30.0	41.0
36	36.21204347923043	38.0	35.0	41.0	30.0	41.0
37	36.1852383023086	38.0	35.0	41.0	30.0	41.0
38	36.08499467589883	38.0	35.0	41.0	30.0	41.0
39	36.05605288978832	38.0	35.0	41.0	30.0	41.0
40	35.950014076812	38.0	35.0	41.0	29.0	41.0
41	35.94232409559967	38.0	35.0	40.0	29.0	41.0
42	35.92397127772853	38.0	35.0	40.0	29.0	41.0
43	35.87800491155302	38.0	35.0	40.0	29.0	41.0
44	35.75045854169802	38.0	35.0	40.0	29.0	41.0
45	35.68760627296193	38.0	34.0	40.0	29.0	41.0
46	35.60909529304856	38.0	34.0	40.0	28.0	41.0
47	35.583501279456776	38.0	34.0	40.0	28.0	41.0
48	35.497682204122135	38.0	34.0	40.0	28.0	41.0
49	35.437952618287035	38.0	34.0	40.0	28.0	41.0
50	35.35651547334326	37.0	34.0	40.0	28.0	41.0
51	34.88691232794233	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	3.0
12	6.0
13	10.0
14	14.0
15	36.0
16	82.0
17	231.0
18	488.0
19	841.0
20	1522.0
21	2502.0
22	3782.0
23	5472.0
24	8315.0
25	12009.0
26	16101.0
27	19718.0
28	22473.0
29	26397.0
30	31423.0
31	39201.0
32	48495.0
33	64249.0
34	109572.0
35	168999.0
36	95967.0
37	127397.0
38	205518.0
39	423840.0
40	315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.20053185261996	25.351502177027758	27.022322200108157	12.425643770244129
2	31.895965390554874	26.257574997351885	27.549645152837943	14.296814459255295
3	30.97212233725254	25.421955924247243	29.88918343340414	13.716738305096085
4	30.17587652545255	26.81256376377716	27.931530943899023	15.080028766871267
5	24.455603686173504	33.54399770311028	26.552142741661232	15.448255869054986
6	22.272861578944433	42.132386145072	25.685094746701008	9.909657529282557
7	84.60909668679233	5.887452403650493	6.704116561578386	2.799334347978793
8	83.44239378278783	6.50669275754991	6.817915739827065	3.232997719835204
9	76.1030785012237	10.829807161612951	8.827276122939349	4.239838214223991
10	40.07041193490659	35.094677013820366	14.782673535035931	10.052237516237113
11	38.09310765834323	23.515384143655957	23.808070333885254	14.58343786411556
12	35.9136408489572	23.352594872137946	25.204880333160506	15.528883945744344
13	24.771844145997445	33.458143087309686	24.418530102077792	17.351482664615077
14	16.564505248839012	37.273307576948596	25.739590127834177	20.42259704637822
15	17.872951893540275	30.396018352817872	35.96360656286063	15.767423190781221
16	19.68098599008769	28.19390320728315	35.67182630607728	16.45328449655188
17	18.95080363265374	27.982820714377304	26.91193769407882	26.154437958890135
18	20.746503096898643	31.120834796764285	28.298573363884195	19.834088742452877
19	26.21834111042353	29.888347187146337	25.489204060811826	18.404107641618303
20	27.604628344288155	28.828126306634775	25.63561684311463	17.931628505962436
21	24.736791490358083	29.61914557932353	27.248666187218813	18.395396743099575
22	23.55956582094295	27.220512563206277	26.48587022573074	22.73405139012003
23	22.455999509402194	29.457122866875167	26.89514308173471	21.191734541987923
24	20.075903285332796	29.410014327685886	32.3015448255869	18.212537561394413
25	19.44878827917245	30.5916999771426	30.22479693153373	19.734714812151218
26	18.971849163474992	35.35579490781779	25.50070244685655	20.17165348185067
27	20.535559978369097	34.11856856940565	27.86741873080118	17.47845272142407
28	17.20785736983827	32.823571552017306	32.94357289001132	17.024998188133107
29	18.521460866462622	29.22130142217614	31.83554659842897	20.42169111293227
30	19.526350119583217	32.325308156746	28.64017996019468	19.50816176347611
31	26.46517313085024	29.660400394708237	24.764317929677265	19.110108544764262
32	26.201825246832023	30.786057544892486	25.6667670162176	17.34535019205789
33	23.61893930524661	31.705231556588785	24.749335184225053	19.926493953939556
34	20.607616530916022	31.838612834707565	26.367750441816774	21.18602019255964
35	22.694538754439073	28.297737117626397	27.988465376617437	21.01925875131709
36	24.47497672447916	31.59192018865716	25.920985878588194	18.012117208275495
37	20.871591599627592	33.91006450246135	27.450410596912576	17.76793330099848
38	19.880848845701415	33.977452013402235	25.10536702848255	21.036332112413795
39	21.70999816025823	31.26515696342259	27.312708713128508	19.712136163190667
40	22.50457147954263	30.53922552446578	27.516683112843072	19.439519883148524
41	18.8855764245455	28.72756769413457	29.118930942784033	23.2679249385359
42	21.253059267559777	28.62645158412916	25.596452643374423	24.524036504936642
43	22.907154365484214	28.053344148784937	26.011300474430378	23.028201011300474
44	20.16712381462093	32.41325338819109	26.49945922741996	20.920163569768025
45	20.378972866596424	35.13216872104497	23.95629498307995	20.532563429278657
46	21.56902097863112	34.75996944913671	24.66159901434441	19.009410557887755
47	22.455860135025894	29.630643965368254	26.599669403979416	21.31382649562643
48	20.770754238374785	27.737730873654343	29.366808271032983	22.12470661693789
49	19.932138616179692	29.56067802846582	29.473778104842978	21.033405250511503
50	19.015543030444938	31.462859516203668	29.74855468771777	19.773042765633626
51	19.549904389177858	30.734767774414212	27.292429741376907	22.422898095031023
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1560.0
1	2387.0
2	3214.0
3	6333.0
4	9452.0
5	7057.0
6	4662.0
7	4653.0
8	4644.0
9	4722.0
10	4800.0
11	4922.0
12	5044.0
13	5016.5
14	4989.0
15	4927.0
16	4865.0
17	4368.0
18	3871.0
19	4156.5
20	4442.0
21	5119.0
22	5796.0
23	6559.0
24	7322.0
25	10352.0
26	16140.0
27	18898.0
28	25978.5
29	33059.0
30	39371.5
31	45684.0
32	44882.0
33	44080.0
34	49420.5
35	54761.0
36	58218.0
37	61675.0
38	62347.5
39	63020.0
40	79293.5
41	95567.0
42	106956.0
43	118345.0
44	125228.0
45	132111.0
46	126223.5
47	120336.0
48	122219.5
49	124103.0
50	111942.0
51	99781.0
52	87663.0
53	75545.0
54	69005.5
55	62466.0
56	59813.5
57	57161.0
58	52646.5
59	48132.0
60	45579.0
61	43026.0
62	33684.0
63	24342.0
64	19327.5
65	14313.0
66	11059.0
67	7805.0
68	6477.0
69	5149.0
70	4216.0
71	3283.0
72	2624.0
73	1965.0
74	1631.5
75	921.5
76	545.0
77	386.0
78	227.0
79	170.0
80	113.0
81	117.5
82	122.0
83	70.5
84	19.0
85	12.5
86	6.0
87	4.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1434984.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.158998332654368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.0342077538436	11.677614268978655
2	9.928478070834664	3.0101156504315627
3	3.692548752576339	1.6792602115064905
4	1.8172969906665657	1.101936082058091
5	1.0120945971238704	0.7671170155144625
6	0.6397743689258097	0.5819003155092811
7	0.43631713985803283	0.4629891556931509
8	0.28666347806677467	0.3476424948837712
9	0.25105170489317724	0.3425123138297139
>10	2.0810906439694152	7.0539077694083785
>50	0.8809630733598669	9.775577472841649
>100	1.910690359629588	57.72541394307803
>500	0.018595526614399883	1.8642765848393412
>1k	0.008832875141839945	2.371184480611224
>5k	0.0013946644960799914	1.2385522408161875
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	6635	0.46237449337414216	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5752	0.40084070623783963	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5188	0.36153713212133376	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3150	0.21951464267197404	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2979	0.2075981334983526	No Hit
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG	2628	0.1831379304577612	No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2248	0.15665679896082466	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2071	0.1443221666583042	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	2067	0.14404341790570488	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2059	0.1434859204005062	No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1965	0.1369353247144219	No Hit
GCCGAAGGGAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACT	1657	0.11547167076427334	No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1549	0.10794545444409137	No Hit
TAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1496	0.10425203347215022	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1473	0.10264922814470405	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06578470561344238	0.0
2	0.0	0.0	0.0	0.2577032217780825	0.0
3	0.0	0.0	0.0	0.3694117843822649	0.0
4	0.0	0.0	0.0	0.5613999877350548	0.0
5	0.0	0.0	0.0	0.957850401119455	0.0
6	0.0	0.0	0.0	1.2834986313436247	0.0
7	0.0	0.0	0.0	1.4746505884386167	0.0
8	0.0	0.0	0.0	1.933610409593417	0.0
9	0.0	0.0	0.0	2.062183271729859	0.0
10	0.0	0.0	0.0	2.3311061308000647	0.0
11	0.0	0.0	0.0	2.7605882713674856	0.0
12	0.0	0.0	0.0	3.085051819393108	0.0
13	0.0	0.0	0.0	3.2276318063476666	0.0
14	0.0	0.0	0.0	3.290141214118067	0.0
15	0.0	0.0	0.0	3.406797567080887	0.0
16	0.0	0.0	0.0	3.6398315242539288	0.0
17	0.0	0.0	0.0	3.9004616079343046	0.0
18	0.0	0.0	0.0	4.19433248036215	0.0
19	0.0	0.0	0.0	4.369456384182681	0.0
20	0.0	0.0	0.0	4.562977705674767	0.0
21	0.0	0.0	0.0	4.802771320098342	0.0
22	0.0	0.0	0.0	5.047373350504256	0.0
23	0.0	0.0	0.0	5.293438811861317	0.0
24	0.0	0.0	0.0	5.489747620879396	0.0
25	0.0	0.0	0.0	5.661874975609484	0.0
26	0.0	0.0	0.0	5.833375145646223	0.0
27	0.0	0.0	0.0	5.998812530313927	0.0
28	0.0	0.0	0.0	6.177629855106399	0.0
29	0.0	0.0	0.0	6.366621509368746	0.0
30	0.0	0.0	0.0	6.584324285148824	0.0
31	0.0	0.0	0.0	6.794918967737619	0.0
32	0.0	0.0	0.0	6.9837015604355175	0.0
33	0.0	0.0	0.0	7.176526010046105	0.0
34	0.0	0.0	0.0	7.379803537879168	0.0
35	0.0	0.0	0.0	7.605799089049077	0.0
36	0.0	0.0	0.0	7.836951492142073	0.0
37	0.0	0.0	0.0	8.055629888556249	0.0
38	0.0	0.0	0.0	8.279604511269811	0.0
39	0.0	0.0	0.0	8.51445033533475	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCC	25	3.8912913E-5	45.000004	28
AGCCGTA	25	3.8912913E-5	45.000004	42
ATCTCGC	25	3.8912913E-5	45.000004	12
AGTCCGC	25	3.8912913E-5	45.000004	25
CGAGTCG	25	3.8912913E-5	45.000004	27
CCGATTC	25	3.8912913E-5	45.000004	37
ACGTTAT	50	2.1827873E-11	45.000004	38
TCCGAGT	25	3.8912913E-5	45.000004	12
CACGCTT	25	3.8912913E-5	45.000004	26
GCACGTA	50	2.1827873E-11	45.000004	9
TCCTCGA	25	3.8912913E-5	45.000004	21
GTTACGT	25	3.8912913E-5	45.000004	25
CCACTAG	50	2.1827873E-11	45.000004	45
CCGTAAA	25	3.8912913E-5	45.000004	30
AAGTTCG	25	3.8912913E-5	45.000004	15
CGTAGAT	25	3.8912913E-5	45.000004	33
CGCTAAT	25	3.8912913E-5	45.000004	40
TATTCCG	25	3.8912913E-5	45.000004	35
TCGCTAA	25	3.8912913E-5	45.000004	39
TCCACGA	25	3.8912913E-5	45.000004	44
>>END_MODULE