Basic Statistics
Measure | Value |
---|---|
Filename | SRR3549277_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3027013 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 10479 | 0.346182854186619 | No Hit |
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 6825 | 0.22546979481092416 | No Hit |
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 4960 | 0.16385790216295734 | No Hit |
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 4559 | 0.15061051934696018 | No Hit |
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC | 3832 | 0.12659344376783319 | No Hit |
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 3334 | 0.1101415818167943 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTATCGA | 30 | 2.166682E-6 | 45.000004 | 31 |
GTCGATA | 50 | 2.1827873E-11 | 45.0 | 32 |
ACGCATC | 35 | 1.2128112E-7 | 45.0 | 32 |
TCGAACG | 35 | 1.2128112E-7 | 45.0 | 1 |
TACGTCG | 65 | 0.0 | 44.999996 | 1 |
ATCGGTA | 95 | 0.0 | 42.63158 | 27 |
CGACGTG | 55 | 6.184564E-11 | 40.909092 | 14 |
CGTTTTT | 6250 | 0.0 | 40.5 | 1 |
CGGTCTA | 575 | 0.0 | 40.304348 | 31 |
CGCATCG | 180 | 0.0 | 40.0 | 21 |
CCAGTCG | 180 | 0.0 | 40.0 | 28 |
CGAACTA | 40 | 3.4607183E-7 | 39.375 | 32 |
CGAATTA | 40 | 3.4607183E-7 | 39.375 | 33 |
TCGAATC | 80 | 0.0 | 39.375 | 17 |
GCGAGAC | 690 | 0.0 | 39.130436 | 21 |
ATGGGAC | 1885 | 0.0 | 38.793102 | 5 |
ACGGTCT | 605 | 0.0 | 38.67769 | 30 |
TGTGCGA | 35 | 6.251672E-6 | 38.571426 | 21 |
AACGGGA | 1190 | 0.0 | 38.571426 | 4 |
CCGTTTA | 35 | 6.251672E-6 | 38.571426 | 29 |