FastQCFastQC Report
Sat 18 Jun 2016
SRR3549277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3027013
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104790.346182854186619No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG68250.22546979481092416No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG49600.16385790216295734No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT45590.15061051934696018No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38320.12659344376783319No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG33340.1101415818167943No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA302.166682E-645.00000431
GTCGATA502.1827873E-1145.032
ACGCATC351.2128112E-745.032
TCGAACG351.2128112E-745.01
TACGTCG650.044.9999961
ATCGGTA950.042.6315827
CGACGTG556.184564E-1140.90909214
CGTTTTT62500.040.51
CGGTCTA5750.040.30434831
CGCATCG1800.040.021
CCAGTCG1800.040.028
CGAACTA403.4607183E-739.37532
CGAATTA403.4607183E-739.37533
TCGAATC800.039.37517
GCGAGAC6900.039.13043621
ATGGGAC18850.038.7931025
ACGGTCT6050.038.6776930
TGTGCGA356.251672E-638.57142621
AACGGGA11900.038.5714264
CCGTTTA356.251672E-638.57142629