##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549276_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1224072
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6116715356613	31.0	31.0	33.0	30.0	34.0
2	32.03677398061552	33.0	31.0	34.0	30.0	34.0
3	32.103248011554875	33.0	31.0	34.0	30.0	34.0
4	35.83595409420361	37.0	35.0	37.0	35.0	37.0
5	35.78543664098191	37.0	35.0	37.0	35.0	37.0
6	35.82692684744035	37.0	35.0	37.0	35.0	37.0
7	36.116792149481405	37.0	35.0	37.0	35.0	37.0
8	36.11789992745525	37.0	35.0	37.0	35.0	37.0
9	38.00812288819612	39.0	39.0	39.0	35.0	39.0
10	37.519246416877436	39.0	37.0	39.0	35.0	39.0
11	37.16366520923606	39.0	37.0	39.0	34.0	39.0
12	36.233039396375375	38.0	35.0	39.0	33.0	39.0
13	35.85394813376991	38.0	35.0	39.0	32.0	39.0
14	36.87817955152965	39.0	35.0	41.0	32.0	41.0
15	37.131181008960255	39.0	35.0	41.0	33.0	41.0
16	37.26892127260488	39.0	35.0	41.0	33.0	41.0
17	37.20765526864433	38.0	35.0	41.0	33.0	41.0
18	37.13654343862126	38.0	35.0	41.0	33.0	41.0
19	37.11802982177519	38.0	35.0	41.0	32.0	41.0
20	36.98089899940526	38.0	35.0	41.0	32.0	41.0
21	36.8170205674176	38.0	35.0	41.0	32.0	41.0
22	36.698056977040565	38.0	35.0	41.0	32.0	41.0
23	36.680184662340125	38.0	35.0	41.0	32.0	41.0
24	36.57919877262122	38.0	35.0	40.0	32.0	41.0
25	36.50481916096439	38.0	35.0	40.0	32.0	41.0
26	36.4316143168049	38.0	35.0	40.0	31.0	41.0
27	36.414273833565346	38.0	35.0	40.0	31.0	41.0
28	36.421298747132525	38.0	35.0	40.0	31.0	41.0
29	36.44887800717605	38.0	35.0	40.0	31.0	41.0
30	36.40850538203635	38.0	35.0	40.0	31.0	41.0
31	36.27404760504284	38.0	35.0	40.0	31.0	41.0
32	36.11863272748662	38.0	35.0	40.0	31.0	41.0
33	36.036342633439865	38.0	35.0	40.0	30.0	41.0
34	35.96852881203066	38.0	35.0	40.0	30.0	41.0
35	35.84077570600422	37.0	35.0	40.0	30.0	41.0
36	35.665113653445225	37.0	35.0	40.0	30.0	41.0
37	35.58110960793156	37.0	35.0	40.0	29.0	41.0
38	35.51719833473848	37.0	34.0	40.0	29.0	41.0
39	35.52686933448359	37.0	35.0	40.0	29.0	41.0
40	35.3184142762844	37.0	34.0	40.0	28.0	41.0
41	35.334263834153546	37.0	34.0	40.0	29.0	41.0
42	35.31677711768589	37.0	34.0	40.0	29.0	41.0
43	35.20629178675764	36.0	34.0	40.0	28.0	41.0
44	35.05477373879968	36.0	34.0	40.0	27.0	41.0
45	34.97356773130992	36.0	34.0	40.0	27.0	41.0
46	34.91967956133299	36.0	34.0	40.0	27.0	41.0
47	34.8550019933468	36.0	34.0	40.0	27.0	41.0
48	34.761255056892075	36.0	34.0	40.0	27.0	41.0
49	34.72893669653419	36.0	34.0	40.0	27.0	41.0
50	34.608945388833334	35.0	34.0	40.0	26.0	41.0
51	34.2092981458607	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	5.0
12	10.0
13	12.0
14	11.0
15	25.0
16	60.0
17	173.0
18	454.0
19	833.0
20	1394.0
21	2361.0
22	3634.0
23	5451.0
24	7753.0
25	11532.0
26	15436.0
27	18327.0
28	20364.0
29	23069.0
30	27213.0
31	33279.0
32	42708.0
33	56871.0
34	112101.0
35	206105.0
36	71183.0
37	91443.0
38	148186.0
39	323939.0
40	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.43722591481547	23.50139534275762	30.532436000496705	13.528942741930214
2	28.701906423805134	26.245024802462602	32.08912547627917	12.96394329745309
3	28.94960427164415	25.173192426589285	31.8537635041076	14.023439797658963
4	25.034638485317856	27.525014868406434	32.15121332732062	15.289133318955095
5	24.173087857577006	33.11676110555588	29.280140383898985	13.430010652968127
6	21.84209752367508	41.787492892574946	25.463534824748873	10.906874759001104
7	89.38420289002607	4.01692057329961	5.32583050670222	1.2730460299720932
8	90.50930010652968	3.2610826814108975	4.719003457312969	1.5106137547464529
9	86.66467331987008	5.75709598781771	5.64239685247273	1.9358338398394865
10	49.671751334888796	33.57302511616964	9.515943506591116	7.23928004235045
11	44.82391558666484	19.288489565973244	22.067656150945368	13.81993869641655
12	42.55002973681287	19.818850525132508	23.343888268010378	14.287231470044246
13	23.11261102288101	39.23265951676046	22.255553594886575	15.399175865471964
14	14.822984268899214	42.667996653791604	25.332251697612556	17.17676737969662
15	13.961923808403428	28.405355240541407	44.58446888745107	13.048252063604101
16	17.582870942232155	22.619012607101542	43.87519688384343	15.922919566822866
17	18.102611611081702	22.50709108614526	27.348472965642546	32.0418243371305
18	21.825513531883743	26.605787894829714	32.20455986249175	19.36413871079479
19	27.685381252083214	26.867455509153054	25.8037108928233	19.643452345940435
20	30.9451568208406	26.23097334143743	25.560261161108173	17.263608676613796
21	24.309436046245644	27.411704540255805	29.23308432837284	19.04577508512571
22	23.443147135135842	24.242854995457783	27.436212902509006	24.877784966897373
23	19.62972766307864	29.538621911129407	26.633482344175835	24.198168081616114
24	19.540762308099524	25.39540157768497	36.975766131404036	18.08806998281147
25	17.068358723996628	27.696491709637993	34.95848283434308	20.2766667320223
26	16.404753968720794	36.58379572443451	27.201749570286715	19.809700736557982
27	17.679760667673143	36.9701292080858	28.772327118012665	16.57778300622839
28	14.417615957231275	32.73516590527355	37.17836859269716	15.66884954479802
29	15.363230267500604	26.04225895208779	37.151981256004554	21.44252952440706
30	17.97974302165232	31.76087681116797	32.07344012443713	18.185940042742583
31	28.718245331973936	28.337467077100044	25.155628100307826	17.7886594906182
32	27.971802312282286	28.530429582573575	26.57915547451457	16.91861263062957
33	25.831323647628572	29.623502539066333	25.28004888601324	19.265124927291858
34	19.23579658712886	30.305243482409534	27.846646275709276	22.612313654752334
35	19.805452620434092	28.889232005960437	29.735995921808524	21.569319451796954
36	29.3494173545347	25.814739655837236	27.109598128214678	17.72624486141338
37	20.57526027880713	32.075237404335695	29.563947218791053	17.785555098066126
38	19.34951538798371	33.116352632851665	25.044360135678296	22.48977184348633
39	20.03893561816625	31.59185080616173	28.58050833611095	19.78870523956107
40	22.948486690325407	29.362651870151428	25.77397407995608	21.914887359567086
41	17.721588272585272	25.819559633747037	29.6926978151612	26.766154278506495
42	21.80108686417139	26.534141782509522	25.174172761079415	26.49059859223967
43	23.110732048441594	25.67781960538269	26.986321066081082	24.225127280094636
44	19.298783078119587	31.72672849309518	27.644043814416147	21.33044461436909
45	17.921821592193922	38.16352306073499	23.22534948924573	20.689305857825357
46	23.17265651040135	33.75691952760948	24.343911142481815	18.72651281950735
47	21.276281133789514	29.95599932030142	27.32102359991896	21.446695945990104
48	23.245773124456733	25.946758033841142	30.00697671378808	20.800492127914044
49	21.75762536844238	25.904521956224798	29.5625584115967	22.77529426373612
50	19.273376075917103	33.03286081210909	26.890820147834443	20.802942964139366
51	18.209468070505658	32.8249482056611	25.322856825415496	23.64272689841774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1139.0
1	1642.5
2	2146.0
3	5849.0
4	9552.0
5	7104.5
6	4657.0
7	4579.5
8	4502.0
9	4673.5
10	4845.0
11	4852.0
12	4859.0
13	4793.0
14	4727.0
15	4530.5
16	4334.0
17	4553.0
18	4772.0
19	4008.5
20	3245.0
21	3827.0
22	4409.0
23	4001.0
24	3593.0
25	5181.5
26	9987.0
27	13204.0
28	14884.0
29	16564.0
30	22728.0
31	28892.0
32	29700.5
33	30509.0
34	35440.0
35	40371.0
36	43509.0
37	46647.0
38	49695.5
39	52744.0
40	71328.0
41	89912.0
42	107636.0
43	125360.0
44	132209.5
45	139059.0
46	128043.0
47	117027.0
48	105008.0
49	92989.0
50	87601.5
51	82214.0
52	75295.0
53	68376.0
54	59239.5
55	50103.0
56	46375.5
57	42648.0
58	39223.0
59	35798.0
60	32754.5
61	29711.0
62	25559.5
63	21408.0
64	18118.5
65	14829.0
66	11802.0
67	8775.0
68	6757.0
69	4739.0
70	3952.5
71	3166.0
72	2895.5
73	2625.0
74	2011.5
75	1115.0
76	832.0
77	628.0
78	424.0
79	252.0
80	80.0
81	69.0
82	58.0
83	38.0
84	18.0
85	20.5
86	23.0
87	15.0
88	7.0
89	6.5
90	6.0
91	5.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1224072.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.841098092948029
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.60139436377042	13.084967907307718
2	7.7006811412088245	2.439744906808079
3	2.1861852934006265	1.0389472704635905
4	0.9426749237663592	0.597320237485808
5	0.511872650059788	0.405431243034718
6	0.3737011969731435	0.3551902391222196
7	0.26005194380729896	0.2883655845779262
8	0.18246662904042127	0.23123774154550977
9	0.1583460501177768	0.225753778129191
>10	1.844987538985161	7.229805789570378
>50	1.0520768814121813	12.302159200905823
>100	2.17726506902402	60.20732830456389
>500	0.005185199021252727	0.5713605974514644
>1k	0.0025925995106263634	0.57776745866498
>5k	5.185199021252728E-4	0.4446197403687143
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5413	0.44221254958858625	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2080	0.1699246449555255	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1529	0.12491095295048005	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.169454084400263E-5	0.0	0.0	0.031125620061565006	0.0
2	8.169454084400263E-5	0.0	0.0	0.11984589141815187	0.0
3	8.169454084400263E-5	0.0	0.0	0.17417276107941362	0.0
4	8.169454084400263E-5	0.0	0.0	0.28666614382160527	0.0
5	8.169454084400263E-5	0.0	0.0	0.465577188269971	0.0
6	8.169454084400263E-5	0.0	0.0	0.6434262036873648	0.0
7	8.169454084400263E-5	0.0	0.0	0.7299407224411636	0.0
8	8.169454084400263E-5	0.0	0.0	0.926906260416054	0.0
9	8.169454084400263E-5	0.0	0.0	0.9858080243645798	0.0
10	8.169454084400263E-5	0.0	0.0	1.0976478507800196	0.0
11	8.169454084400263E-5	0.0	0.0	1.2854636001803816	0.0
12	8.169454084400263E-5	0.0	0.0	1.427203628544726	0.0
13	8.169454084400263E-5	0.0	0.0	1.487004032442536	0.0
14	8.169454084400263E-5	0.0	0.0	1.512574423726709	0.0
15	8.169454084400263E-5	0.0	0.0	1.5564443921599382	0.0
16	8.169454084400263E-5	0.0	0.0	1.6490043069361933	0.0
17	8.169454084400263E-5	0.0	0.0	1.764847165852989	0.0
18	8.169454084400263E-5	0.0	0.0	1.8987445182963094	0.0
19	8.169454084400263E-5	0.0	0.0	1.973576717709416	0.0
20	8.169454084400263E-5	0.0	0.0	2.053147200491474	0.0
21	8.169454084400263E-5	0.0	0.0	2.1523243730760937	0.0
22	8.169454084400263E-5	0.0	0.0	2.258608970714141	0.0
23	8.169454084400263E-5	0.0	0.0	2.3750236914168448	0.0
24	8.169454084400263E-5	0.0	0.0	2.4574534831284436	0.0
25	8.169454084400263E-5	0.0	0.0	2.5319589043781736	0.0
26	8.169454084400263E-5	0.0	0.0	2.6104673581292603	0.0
27	8.169454084400263E-5	0.0	0.0	2.6785189106523144	0.0
28	8.169454084400263E-5	0.0	0.0	2.758742949761125	0.0
29	8.169454084400263E-5	0.0	0.0	2.8417446032586318	0.0
30	8.169454084400263E-5	0.0	0.0	2.9582410185021795	0.0
31	8.169454084400263E-5	0.0	0.0	3.064689005221915	0.0
32	8.169454084400263E-5	0.0	0.0	3.156758752753106	0.0
33	8.169454084400263E-5	0.0	0.0	3.2451522459463167	0.0
34	8.169454084400263E-5	0.0	0.0	3.34155180414224	0.0
35	8.169454084400263E-5	0.0	0.0	3.461642779182924	0.0
36	8.169454084400263E-5	0.0	0.0	3.5666202641674674	0.0
37	8.169454084400263E-5	0.0	0.0	3.6724146945604508	0.0
38	8.169454084400263E-5	0.0	0.0	3.777555568626682	0.0
39	8.169454084400263E-5	0.0	0.0	3.888006587847774	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCAA	20	7.0334657E-4	45.000004	15
CAAGTCG	20	7.0334657E-4	45.000004	1
GCGGTAT	20	7.0334657E-4	45.000004	36
CTATCGG	20	7.0334657E-4	45.000004	21
GCGAAGT	20	7.0334657E-4	45.000004	44
GCAACGC	20	7.0334657E-4	45.000004	9
ACCGCCC	20	7.0334657E-4	45.000004	28
GGCGTAA	20	7.0334657E-4	45.000004	20
GCTATCG	20	7.0334657E-4	45.000004	20
CGATTGA	20	7.0334657E-4	45.000004	10
CGCGAGA	20	7.0334657E-4	45.000004	45
CCTCGGT	20	7.0334657E-4	45.000004	43
TTATCCG	20	7.0334657E-4	45.000004	1
ACCTCGC	20	7.0334657E-4	45.000004	44
CCCGTCG	20	7.0334657E-4	45.000004	12
GTTCGGT	20	7.0334657E-4	45.000004	17
CTACGCT	20	7.0334657E-4	45.000004	29
CGAGGTA	40	6.8157533E-9	45.000004	42
TTACGCT	20	7.0334657E-4	45.000004	28
GCGTGAG	20	7.0334657E-4	45.000004	30
>>END_MODULE