FastQCFastQC Report
Sat 18 Jun 2016
SRR3549275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789172
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101470.5671338473886244No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36560.20434033172886676No Hit
GAGCTGTCTCTTATACACATCTGACGCGCCATGAATCGTATGCCGTCTTCT20790.11619900154932002No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT20760.11603132622240901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAA351.2124292E-745.00000419
CCACTCG351.2124292E-745.00000426
CGGATTA351.2124292E-745.00000429
GTCCGTG351.2124292E-745.00000442
AACGAGT302.1661817E-645.00000417
TACTCGA302.1661817E-645.00000415
TTACGTC302.1661817E-645.00000421
CGTAACA302.1661817E-645.00000417
GTCGAAC253.891679E-545.017
ACACCGA207.034341E-445.018
CGCGACT207.034341E-445.043
ATCCGAT253.891679E-545.026
TCGGATC207.034341E-445.041
AGTGCGT207.034341E-445.029
ACGATAA207.034341E-445.016
ACTAACG207.034341E-445.020
CGTAACT253.891679E-545.033
CCGCGAC207.034341E-445.042
GCTCGAA207.034341E-445.042
TCGACTA207.034341E-445.029