##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549275_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1789172
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.221050854808816	31.0	31.0	33.0	30.0	34.0
2	31.617799183085808	31.0	31.0	34.0	30.0	34.0
3	31.764762694699	31.0	31.0	34.0	30.0	34.0
4	35.50722680658986	37.0	35.0	37.0	33.0	37.0
5	35.377877029150916	37.0	35.0	37.0	33.0	37.0
6	35.42819751259242	37.0	35.0	37.0	33.0	37.0
7	35.86414889121895	37.0	35.0	37.0	35.0	37.0
8	35.89435336569095	37.0	35.0	37.0	35.0	37.0
9	37.60872906573543	39.0	37.0	39.0	35.0	39.0
10	36.937803632071144	39.0	37.0	39.0	32.0	39.0
11	36.75573729076914	39.0	35.0	39.0	32.0	39.0
12	36.15653721386205	38.0	35.0	39.0	32.0	39.0
13	35.904976156568516	38.0	35.0	39.0	31.0	39.0
14	36.98114323273559	39.0	35.0	41.0	31.0	41.0
15	37.180863550290304	39.0	35.0	41.0	32.0	41.0
16	37.26590512259302	39.0	35.0	41.0	32.0	41.0
17	37.19549545823431	39.0	35.0	41.0	32.0	41.0
18	37.09139199585059	39.0	35.0	41.0	32.0	41.0
19	37.0407691379029	39.0	35.0	41.0	32.0	41.0
20	36.91844719233254	38.0	35.0	41.0	31.0	41.0
21	36.83797589052366	38.0	35.0	41.0	31.0	41.0
22	36.70609980482592	38.0	35.0	41.0	31.0	41.0
23	36.6538387589343	38.0	35.0	40.0	31.0	41.0
24	36.589484409548106	38.0	35.0	40.0	31.0	41.0
25	36.487750199533636	38.0	35.0	40.0	31.0	41.0
26	36.329332227421396	38.0	35.0	40.0	30.0	41.0
27	36.23851647577762	38.0	35.0	40.0	30.0	41.0
28	36.14981678675946	38.0	35.0	40.0	30.0	41.0
29	36.193896953451095	38.0	35.0	40.0	30.0	41.0
30	36.15342292412356	38.0	35.0	40.0	30.0	41.0
31	36.04606544256226	38.0	35.0	40.0	30.0	41.0
32	35.927253500501905	38.0	35.0	40.0	30.0	41.0
33	35.83382145484056	38.0	35.0	40.0	29.0	41.0
34	35.720751274891406	38.0	35.0	40.0	29.0	41.0
35	35.61070148649767	38.0	34.0	40.0	28.0	41.0
36	35.54439371955296	38.0	34.0	40.0	27.0	41.0
37	35.480013101032206	38.0	34.0	40.0	27.0	41.0
38	35.38500658405117	38.0	34.0	40.0	27.0	41.0
39	35.29776790604816	38.0	34.0	40.0	27.0	41.0
40	35.14492457963796	38.0	34.0	40.0	25.0	41.0
41	35.06114113120483	38.0	34.0	40.0	25.0	41.0
42	35.00503864357368	38.0	34.0	40.0	25.0	41.0
43	34.96176723087551	38.0	34.0	40.0	25.0	41.0
44	34.844158638744624	37.0	34.0	40.0	24.0	41.0
45	34.70282845919789	37.0	34.0	40.0	24.0	41.0
46	34.644244935646206	37.0	34.0	40.0	24.0	41.0
47	34.59218006988708	37.0	33.0	40.0	24.0	41.0
48	34.540119675469995	37.0	33.0	40.0	24.0	41.0
49	34.48052283402602	37.0	33.0	40.0	24.0	41.0
50	34.3525971790303	37.0	33.0	40.0	24.0	41.0
51	33.77069113534082	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	7.0
14	16.0
15	62.0
16	123.0
17	396.0
18	812.0
19	1539.0
20	2829.0
21	4373.0
22	6640.0
23	9891.0
24	14567.0
25	20377.0
26	26646.0
27	31867.0
28	36033.0
29	41815.0
30	49321.0
31	58173.0
32	71099.0
33	88882.0
34	137188.0
35	196975.0
36	137410.0
37	168386.0
38	249239.0
39	434266.0
40	239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.51807428240549	24.51625668186178	28.495806998991714	13.469862036741018
2	28.08092234843827	27.81761619341237	30.803298956165197	13.298162501984159
3	28.88822315573908	26.629860069350514	31.447451670381604	13.0344651045288
4	24.90688430178876	30.051610465623202	30.3463277985571	14.695177434030937
5	23.052618753255697	33.21888560742064	29.41645632728435	14.312039312039312
6	21.7493902207278	42.65548533064457	25.472732638337735	10.1223918102899
7	88.70438392731387	3.8599419172667577	6.104276167970435	1.331397987448943
8	88.33410091371874	3.570981437223476	5.443970730594935	2.650946918462842
9	82.51716436429813	7.297397902493444	7.586246598985452	2.5991911342229814
10	32.14134806491494	45.27608301493652	11.659918666288092	10.922650253860446
11	36.813900508168025	23.64065612473256	23.964157722119506	15.58128564497991
12	35.11909419552732	22.459830580849687	25.030237450619612	17.39083777300338
13	23.698112870087392	33.17635196616088	26.233028462327823	16.892506701423898
14	17.946737373488965	35.427337338165366	28.27944993550089	18.34647535284478
15	17.60272349444324	26.950734753282525	37.57514649234395	17.871395259930292
16	20.395300172370238	24.601044505503104	37.07217640338659	17.931478918740066
17	19.407245362659374	25.780025620789953	27.443811997952128	27.368917018598548
18	21.202433304344133	26.36387110909404	31.681694102076268	20.75200148448556
19	25.02576610856866	27.28284368411757	26.90853646267659	20.782853744637183
20	27.359471308515893	26.70447558982591	26.91149872678535	19.024554374872846
21	24.242386981240486	28.38094940005768	28.566398311621242	18.81026530708059
22	23.128855135224562	25.26252367016698	27.379256997091396	24.229364197517064
23	19.905576434238856	29.915625775498388	27.175810933772716	23.00298685649004
24	20.27250594129575	25.829825192882517	34.51971079359614	19.37795807222559
25	19.68200933169086	27.755185080025846	31.09063857471501	21.47216701356829
26	19.603146036267056	32.92863961653771	26.849570639379554	20.618643707815682
27	19.883275615759693	32.13827401725491	28.252733666746405	19.725716700238994
28	18.10323434527256	29.849226346041636	32.26676920944437	19.780770099241437
29	18.43763483890872	26.37739691879819	32.30606112771718	22.878907114575906
30	19.708334358015886	30.224763186546628	29.34664749951374	20.720254955923746
31	24.653918125255707	28.58076249795995	26.308873601867234	20.45644577491711
32	24.966967960598534	28.392798456492724	27.95823990091506	18.681993681993685
33	24.300737995005512	28.57886217758829	26.526236717319517	20.594163110086676
34	20.25204955141261	28.979494425354297	28.01239903150731	22.75605699172578
35	20.447670766142103	28.058509746407832	29.04907968602236	22.444739801427698
36	25.07562157243686	28.083884612547035	27.198838345335158	19.641655469680945
37	19.940229335133793	32.156997762093305	27.370258421213833	20.532514481559065
38	19.856894697659026	32.48726226433233	26.195636864426675	21.46020617358197
39	20.72617948414127	30.473928722336364	27.274739376650203	21.52515241687216
40	23.57353010219252	27.93426232916679	26.966216775133972	21.525990793506715
41	19.071615249959198	26.861196128712052	28.999168330378524	25.068020290950226
42	20.84936495764521	27.98803021732958	26.997907411920153	24.164697413105056
43	21.31248421057338	26.65937092688685	28.308793117710312	23.71935174482945
44	20.08090893441212	28.42504801103527	28.299235624076392	23.19480743047622
45	19.620248919611978	31.84646305665414	26.076028464563493	22.457259559170385
46	21.124743736208703	30.91933028238761	26.672170143507724	21.283755837895963
47	21.228311196464063	28.323436762927205	27.74428618377663	22.7039658568321
48	21.23675085458525	26.907698086042036	30.052616517584667	21.802934541788044
49	20.93113462540214	24.890060877322025	31.27793191487459	22.900872582401245
50	20.24998155571404	29.138618310592832	29.13699744909936	21.47440268459377
51	19.178424433201503	29.337257681206726	27.52513453150396	23.959183354087813
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1419.0
1	2051.5
2	2684.0
3	9149.5
4	15615.0
5	10977.0
6	6339.0
7	6698.5
8	7058.0
9	7725.0
10	8392.0
11	8992.5
12	9593.0
13	9570.5
14	9548.0
15	9427.0
16	9306.0
17	8958.0
18	8610.0
19	8519.5
20	8429.0
21	9068.0
22	9707.0
23	9594.0
24	9481.0
25	10810.5
26	16422.5
27	20705.0
28	22261.5
29	23818.0
30	29071.0
31	34324.0
32	39008.5
33	43693.0
34	49071.0
35	54449.0
36	60200.0
37	65951.0
38	71851.5
39	77752.0
40	98889.0
41	120026.0
42	129474.5
43	138923.0
44	143830.0
45	148737.0
46	157746.0
47	166755.0
48	161160.5
49	155566.0
50	148798.0
51	142030.0
52	125848.5
53	109667.0
54	98847.0
55	88027.0
56	81734.0
57	75441.0
58	68667.0
59	61893.0
60	56243.0
61	50593.0
62	42001.0
63	33409.0
64	28376.5
65	23344.0
66	18526.5
67	13709.0
68	10921.5
69	8134.0
70	6679.5
71	5225.0
72	4342.0
73	3459.0
74	2697.0
75	1979.0
76	2023.0
77	1361.5
78	700.0
79	562.0
80	424.0
81	237.5
82	51.0
83	62.5
84	74.0
85	40.0
86	6.0
87	5.0
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1789172.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.89153443737852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.03185944005065	14.14001234821725
2	8.84027484943636	3.163321638091637
3	3.11143347950597	1.670049577445806
4	1.4925618409237296	1.0681688630721595
5	0.8680477685400849	0.7765353272062254
6	0.5689613587573602	0.6107755046246991
7	0.4011950983996832	0.5024597143387777
8	0.2801383637026608	0.40096841451336196
9	0.22856157841171848	0.36803856160933673
>10	2.306306457785874	10.024524739085404
>50	1.2149471764556492	16.05535656760626
>100	1.6433989360598706	48.64216024331711
>500	0.00852483597955996	0.9946719842944071
>1k	0.00347308132500591	1.0097547546150911
>5k	0.0	0.0
>10k+	3.157346659096282E-4	0.57320176196248
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10147	0.5671338473886244	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3656	0.20434033172886676	No Hit
GAGCTGTCTCTTATACACATCTGACGCGCCATGAATCGTATGCCGTCTTCT	2079	0.11619900154932002	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2076	0.11603132622240901	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08702349466680677	0.0
2	0.0	0.0	0.0	0.32808472298918157	0.0
3	0.0	0.0	0.0	0.47865716655525575	0.0
4	0.0	0.0	0.0	0.8886792326282772	0.0
5	0.0	0.0	0.0	1.3494510309796934	0.0
6	0.0	0.0	0.0	1.9577212252371488	0.0
7	0.0	0.0	0.0	2.2016329341170104	0.0
8	0.0	0.0	0.0	2.9527625069026344	0.0
9	0.0	0.0	0.0	3.1711875660920246	0.0
10	0.0	0.0	0.0	3.6139622126883273	0.0
11	0.0	0.0	0.0	4.151920553194438	0.0
12	0.0	0.0	0.0	4.603805559219572	0.0
13	0.0	0.0	0.0	4.775505093976432	0.0
14	0.0	0.0	0.0	4.850344181554373	0.0
15	0.0	0.0	0.0	4.9728589537506735	0.0
16	0.0	0.0	0.0	5.224371944117167	0.0
17	0.0	0.0	0.0	5.519759978358705	0.0
18	0.0	0.0	0.0	5.868971792538671	0.0
19	0.0	0.0	0.0	6.046428180186142	0.0
20	0.0	0.0	0.0	6.23975783211452	0.0
21	0.0	0.0	0.0	6.486352346224957	0.0
22	0.0	0.0	0.0	6.7541298433018175	0.0
23	0.0	0.0	0.0	7.020733613090301	0.0
24	0.0	0.0	0.0	7.223453083325695	0.0
25	0.0	0.0	0.0	7.401691955832083	0.0
26	0.0	0.0	0.0	7.57009387582636	0.0
27	0.0	0.0	0.0	7.734248020872225	0.0
28	0.0	0.0	0.0	7.9130458111349835	0.0
29	0.0	0.0	0.0	8.104642818018615	0.0
30	5.589177563699857E-5	0.0	0.0	8.34235053980277	0.0
31	5.589177563699857E-5	0.0	0.0	8.556695499370658	0.0
32	5.589177563699857E-5	0.0	0.0	8.74197673560731	0.0
33	5.589177563699857E-5	0.0	0.0	8.946149392009264	0.0
34	5.589177563699857E-5	0.0	0.0	9.14512411327698	0.0
35	5.589177563699857E-5	0.0	0.0	9.38422912945206	0.0
36	5.589177563699857E-5	0.0	0.0	9.607237314243683	0.0
37	5.589177563699857E-5	0.0	0.0	9.816104879799147	0.0
38	5.589177563699857E-5	0.0	0.0	10.033523887027071	0.0
39	5.589177563699857E-5	0.0	0.0	10.260388604337649	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAA	35	1.2124292E-7	45.000004	19
CCACTCG	35	1.2124292E-7	45.000004	26
CGGATTA	35	1.2124292E-7	45.000004	29
GTCCGTG	35	1.2124292E-7	45.000004	42
AACGAGT	30	2.1661817E-6	45.000004	17
TACTCGA	30	2.1661817E-6	45.000004	15
TTACGTC	30	2.1661817E-6	45.000004	21
CGTAACA	30	2.1661817E-6	45.000004	17
GTCGAAC	25	3.891679E-5	45.0	17
ACACCGA	20	7.034341E-4	45.0	18
CGCGACT	20	7.034341E-4	45.0	43
ATCCGAT	25	3.891679E-5	45.0	26
TCGGATC	20	7.034341E-4	45.0	41
AGTGCGT	20	7.034341E-4	45.0	29
ACGATAA	20	7.034341E-4	45.0	16
ACTAACG	20	7.034341E-4	45.0	20
CGTAACT	25	3.891679E-5	45.0	33
CCGCGAC	20	7.034341E-4	45.0	42
GCTCGAA	20	7.034341E-4	45.0	42
TCGACTA	20	7.034341E-4	45.0	29
>>END_MODULE