FastQCFastQC Report
Sat 18 Jun 2016
SRR3549273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2098428
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121410.578575962577701No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35520.1692695675048179No Hit
GAGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCT27050.12890601917244718No Hit
AAGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCT22190.10574582496992986No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA21750.10364901726435218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGA406.8193913E-945.0000048
CATGCGT302.1663618E-645.00000430
CGCATCG351.2125747E-745.00000421
GTCACGA207.0346193E-445.00000441
CGTAAGA207.0346193E-445.00000434
CGTAAAC207.0346193E-445.00000438
ACCGTTA406.8193913E-945.00000435
ACGTATC207.0346193E-445.00000435
AACTCGC207.0346193E-445.00000416
CACCGTA302.1663618E-645.00000440
GCCCGTA302.1663618E-645.00000414
GCGTACA207.0346193E-445.00000412
CCGAATA253.8919105E-545.041
CGACGGT4950.043.63636428
TATAGCG950.042.631581
CGGTCTA5200.042.40384331
ATACGAA3650.041.91780519
CATACGA3850.041.49350718
CGTTTTT63250.041.478261
CGATCAT603.6379788E-1241.25000410