FastQCFastQC Report
Sat 18 Jun 2016
SRR3549271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432625
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT535982.2032989055033143No Hit
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT40630.16702122193104157Illumina PCR Primer Index 11 (96% over 25bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT32660.1342582601099635No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT30260.1243923744925749No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT30070.12361132521453162No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC28050.11530753815322953No Hit
AAGAGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT27590.11341657674323005No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC26580.109264683212579Illumina PCR Primer Index 11 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26150.10749704537279688No Hit
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG26120.10737372180257951Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT24570.10100200400801602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAA406.8212103E-945.028
TACCTCG207.034843E-445.029
CGTAAAT351.2126839E-745.016
TAAATCG207.034843E-445.035
GCGTACT207.034843E-445.018
CTTACCG207.034843E-445.01
CGGTCGA302.1665055E-644.99999640
CGTTTTT156250.043.6321
CGCATCG3200.043.5937521
CGGTCTA10700.042.89719431
ACGGGTA1700.042.352945
CCGCATC3350.042.31343520
TGTTACG700.041.7857131
CGACGGT11050.041.74208528
ACGGTCT11000.041.72727230
ATAACGC3650.041.30137311
TCACGAC11550.041.29870225
TAGCACG603.6379788E-1241.2499961
CGTTTCG603.6379788E-1241.24999642
TGCGTAG2050.040.6097561
Produced by FastQC (version 0.11.5)