##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549271_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2432625
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.131736704177587	31.0	31.0	33.0	30.0	34.0
2	31.524355788500078	31.0	31.0	34.0	30.0	34.0
3	31.574592055906685	31.0	31.0	34.0	30.0	34.0
4	35.3842092389908	37.0	35.0	37.0	33.0	37.0
5	35.25679255947793	37.0	35.0	37.0	33.0	37.0
6	35.3055152355994	37.0	35.0	37.0	32.0	37.0
7	35.812385386156926	37.0	35.0	37.0	35.0	37.0
8	35.851672164842505	37.0	35.0	37.0	35.0	37.0
9	37.54238610554442	39.0	37.0	39.0	35.0	39.0
10	36.8470356096809	39.0	37.0	39.0	32.0	39.0
11	36.6558084373876	39.0	35.0	39.0	32.0	39.0
12	36.33336334206875	38.0	35.0	39.0	32.0	39.0
13	36.23636031036432	38.0	35.0	39.0	32.0	39.0
14	37.34549098196393	39.0	36.0	41.0	32.0	41.0
15	37.45355284928832	39.0	36.0	41.0	32.0	41.0
16	37.48973680694723	39.0	36.0	41.0	32.0	41.0
17	37.418389188633675	39.0	36.0	41.0	32.0	41.0
18	37.298805200143875	39.0	36.0	41.0	32.0	41.0
19	37.17922737783259	39.0	36.0	41.0	32.0	41.0
20	37.005249884384156	39.0	35.0	41.0	31.0	41.0
21	36.94075617902472	39.0	35.0	41.0	31.0	41.0
22	36.86620461435692	38.0	35.0	41.0	31.0	41.0
23	36.75593237757566	38.0	35.0	41.0	31.0	41.0
24	36.631827347001696	38.0	35.0	40.0	31.0	41.0
25	36.50879153178151	38.0	35.0	40.0	31.0	41.0
26	36.30893705359437	38.0	35.0	40.0	30.0	41.0
27	36.16375191408458	38.0	35.0	40.0	30.0	41.0
28	36.00316571604748	38.0	35.0	40.0	30.0	41.0
29	35.92518205642053	38.0	35.0	40.0	30.0	41.0
30	35.806891732182315	38.0	35.0	40.0	30.0	41.0
31	35.6590481475772	38.0	34.0	40.0	29.0	41.0
32	35.490087045886646	38.0	34.0	40.0	28.0	41.0
33	35.303432711577	38.0	34.0	40.0	27.0	41.0
34	35.12492842094445	38.0	34.0	40.0	25.0	41.0
35	34.977453573814294	37.0	34.0	40.0	25.0	41.0
36	34.871753352859564	37.0	34.0	40.0	24.0	41.0
37	34.79880704999743	37.0	34.0	40.0	24.0	41.0
38	34.68664015209907	37.0	33.0	40.0	24.0	41.0
39	34.58948049946046	37.0	33.0	40.0	23.0	41.0
40	34.45351133035302	37.0	33.0	40.0	23.0	41.0
41	34.29678680437799	37.0	33.0	40.0	23.0	41.0
42	34.2322848774472	37.0	33.0	40.0	23.0	41.0
43	34.162142541493246	37.0	33.0	40.0	22.0	41.0
44	34.02226113765993	36.0	33.0	40.0	22.0	41.0
45	33.86885648219516	36.0	33.0	40.0	22.0	41.0
46	33.797801140743026	36.0	33.0	40.0	22.0	41.0
47	33.71860233287087	36.0	33.0	40.0	22.0	41.0
48	33.60616946713941	36.0	33.0	40.0	21.0	41.0
49	33.53860048301732	36.0	33.0	40.0	20.0	41.0
50	33.41073120600175	35.0	32.0	40.0	20.0	41.0
51	32.91095750475309	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	5.0
12	6.0
13	5.0
14	28.0
15	105.0
16	250.0
17	611.0
18	1331.0
19	2647.0
20	4723.0
21	7229.0
22	11380.0
23	16589.0
24	24483.0
25	34729.0
26	43689.0
27	49536.0
28	53847.0
29	61047.0
30	70563.0
31	82880.0
32	98607.0
33	122009.0
34	173813.0
35	246933.0
36	249260.0
37	248313.0
38	311247.0
39	516460.0
40	294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.782138636246852	25.095195519243614	28.133066132264528	15.989599712245003
2	32.74175016700067	26.778356713426852	27.856574687837217	12.623318431735264
3	26.852104208416833	25.72036380453214	34.2067519654694	13.220780021581625
4	23.967113714608704	28.240275422640153	33.23701762499358	14.555593237757567
5	22.64825034684754	31.417378346436465	31.96349622321566	13.970875083500333
6	19.88888546323416	40.599640306253534	28.82787112686912	10.683603103643184
7	84.33264477673296	3.6470479420379216	9.9715739170649	2.048733364164226
8	83.78833564565028	3.382395560351472	9.457273521401778	3.3719952725964752
9	77.65520785160064	6.612856482195159	12.2856173886234	3.4463182775808026
10	30.041005087097272	37.68357227275063	17.597122450028262	14.678300190123839
11	27.19506705719131	24.983176609629513	29.50586300806742	18.315893325111762
12	27.908822773752632	23.703735676481166	28.865690355069113	19.52175119469709
13	21.730394121576484	30.368182518883923	29.777421509686036	18.124001849853553
14	16.808386002774782	34.163999794460715	29.402435640511793	19.62517856225271
15	15.387246287446688	31.059082267098297	35.85875340424439	17.694918041210627
16	18.06361440830379	28.72193618005241	35.0870356096809	18.1274138019629
17	16.672812291249166	31.625301885822925	28.984738708185603	22.717147114742307
18	18.131113509069422	31.570381789219464	30.98299162427419	19.315513077436925
19	21.011294383639072	34.46174400082216	26.12737269410616	18.39958892143261
20	22.71554390832948	31.823359539592005	26.742510662350345	18.718585889728175
21	19.31156672318997	34.531504033708444	27.968799136735008	18.18813010636658
22	19.60224037819228	31.184502338009352	26.995447304866143	22.217809978932223
23	17.55280818046349	34.154709418837676	27.108904989466108	21.183577411232722
24	17.931699296027954	32.16081393556344	32.42131442371923	17.48617234468938
25	18.716530496891217	33.94838908586404	27.636812085709884	19.698268331534866
26	17.18374184265968	36.68867992395046	26.405796207800215	19.72178202558964
27	17.361368891629414	35.95437027901958	27.500457324906225	19.18380350444479
28	16.83966908175325	34.656101947484714	29.74141102718257	18.762817943579467
29	17.337937413288117	31.751420790298546	29.47207234982786	21.43856944658548
30	19.484836339345357	34.74345614305534	28.362859051436207	17.408848466163096
31	20.71552335440111	34.12042546631725	26.962026617337237	18.202024561944402
32	21.971820564205334	33.643039926005855	26.32341606289502	18.061723446893787
33	20.564493088741585	34.20177791480397	26.056338317660966	19.177390678793486
34	17.741164380042136	33.497148142438725	27.837829505164173	20.923857972354966
35	18.383104670880222	33.914516211911	27.443646266892756	20.258732850316015
36	20.755521299008272	33.70963465392323	26.354082524022402	19.18076152304609
37	17.09729201993731	37.367370638713325	27.604295771029236	17.931041570320126
38	18.241652535840913	35.492441292842095	27.12070294435024	19.145203226966753
39	17.14904681157186	35.34338420430605	25.453573814295254	22.053995169826834
40	20.407173321000975	33.12845177534556	27.213894455577826	19.25048044807564
41	17.323919634140076	32.246523816864496	28.23941215764863	22.1901443913468
42	18.781645341965984	33.03600020553928	26.453522429474337	21.7288320230204
43	18.098761625815733	32.70043677097785	28.623359539592002	20.57744206361441
44	18.94188376753507	34.020574482297924	26.655444221776886	20.382097528390116
45	17.53237757566415	35.12037408149632	25.826997584913418	21.52025075792611
46	19.28040696778172	34.71644828117774	26.112820512820512	19.890324238220032
47	18.536519192230617	33.23857972354966	27.335491495812136	20.889409588407585
48	17.389157802785057	32.6953393967422	29.74424746929757	20.17125533117517
49	18.46079852011716	31.652021992703354	28.424315297261188	21.4628641899183
50	17.745069626432354	32.37720569343816	29.629679872565646	20.248044807563844
51	16.303170443451005	32.242988541184936	28.947782744977136	22.506058270386927
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3248.0
1	3787.5
2	4327.0
3	33589.0
4	62851.0
5	38416.0
6	13981.0
7	15601.5
8	17222.0
9	23523.5
10	29825.0
11	35860.5
12	41896.0
13	44705.0
14	47514.0
15	45380.0
16	43246.0
17	39062.0
18	34878.0
19	31186.0
20	27494.0
21	25939.0
22	24384.0
23	24191.5
24	23999.0
25	23967.5
26	25981.0
27	28026.0
28	33297.5
29	38569.0
30	43928.0
31	49287.0
32	53989.5
33	58692.0
34	66348.0
35	74004.0
36	79022.5
37	84041.0
38	88968.0
39	93895.0
40	105922.5
41	117950.0
42	133669.0
43	149388.0
44	158660.0
45	167932.0
46	190003.5
47	212075.0
48	219626.0
49	227177.0
50	208159.5
51	189142.0
52	159084.0
53	129026.0
54	113253.0
55	97480.0
56	90610.0
57	83740.0
58	77268.5
59	70797.0
60	63756.0
61	56715.0
62	47314.5
63	37914.0
64	31413.0
65	24912.0
66	20085.0
67	15258.0
68	12427.0
69	9596.0
70	8285.5
71	6975.0
72	5839.0
73	4703.0
74	3663.0
75	2159.0
76	1695.0
77	1426.5
78	1158.0
79	827.0
80	496.0
81	371.0
82	246.0
83	227.0
84	208.0
85	129.0
86	50.0
87	39.0
88	28.0
89	19.5
90	11.0
91	9.5
92	8.0
93	7.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2432625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.92552810198802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.07826119161099	17.212087867966744
2	8.945192391261415	4.1014651908710595
3	3.796969572105132	2.611425978830689
4	2.3289253654363793	2.1356737565097776
5	1.593863348551998	1.8270079493978777
6	1.119737425876199	1.5402343094263518
7	0.8317140275595275	1.334723831814351
8	0.627158999606451	1.150236101591391
9	0.46908741216495337	0.9678668984888829
>10	3.447619154272637	17.076703067673954
>50	0.9649408763114188	15.859608175981974
>100	0.7878071329889885	29.186590102276426
>500	0.004361551126984163	0.7027691149592121
>1k	0.00417981983002649	2.0605617078285934
>5k	0.0	0.0
>10k+	1.8173129695767346E-4	2.2330459463827275
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53598	2.2032989055033143	No Hit
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	4063	0.16702122193104157	Illumina PCR Primer Index 11 (96% over 25bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3266	0.1342582601099635	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	3026	0.1243923744925749	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	3007	0.12361132521453162	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2805	0.11530753815322953	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	2759	0.11341657674323005	No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	2658	0.109264683212579	Illumina PCR Primer Index 11 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2615	0.10749704537279688	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	2612	0.10737372180257951	Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	2457	0.10100200400801602	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.110785673911926E-5	0.0	0.0	0.1841631981912543	0.0
2	4.110785673911926E-5	0.0	0.0	0.47208262679204566	0.0
3	4.110785673911926E-5	0.0	0.0	0.7489440419300138	0.0
4	4.110785673911926E-5	0.0	0.0	1.2101330866861928	0.0
5	4.110785673911926E-5	0.0	0.0	1.976013565592724	0.0
6	4.110785673911926E-5	0.0	0.0	3.367267869071476	0.0
7	4.110785673911926E-5	0.0	0.0	4.1101690560608395	0.0
8	4.110785673911926E-5	0.0	0.0	5.638106983197163	0.0
9	4.110785673911926E-5	0.0	0.0	6.244447870099172	0.0
10	4.110785673911926E-5	0.0	0.0	7.1133857458506755	0.0
11	4.110785673911926E-5	0.0	0.0	8.022732644776733	0.0
12	4.110785673911926E-5	0.0	0.0	8.717414315811109	0.0
13	4.110785673911926E-5	0.0	0.0	9.050963465392323	0.0
14	4.110785673911926E-5	0.0	0.0	9.209269821694672	0.0
15	4.110785673911926E-5	0.0	0.0	9.384471507116798	0.0
16	4.110785673911926E-5	0.0	0.0	9.707990339653666	0.0
17	4.110785673911926E-5	0.0	0.0	10.085894866656389	0.0
18	4.110785673911926E-5	0.0	0.0	10.502358563280406	0.0
19	8.221571347823852E-5	0.0	0.0	10.731452648887519	0.0
20	8.221571347823852E-5	0.0	0.0	10.964082010174195	0.0
21	8.221571347823852E-5	0.0	0.0	11.269801140743024	0.0
22	8.221571347823852E-5	0.0	0.0	11.58090540054468	0.0
23	8.221571347823852E-5	0.0	0.0	11.91038487230872	0.0
24	8.221571347823852E-5	0.0	0.0	12.165294691947999	0.0
25	8.221571347823852E-5	0.0	0.0	12.371573917064898	0.0
26	8.221571347823852E-5	0.0	0.0	12.558779096654849	0.0
27	8.221571347823852E-5	0.0	0.0	12.751821591901752	0.0
28	8.221571347823852E-5	0.0	0.0	12.957401983454087	0.0
29	8.221571347823852E-5	0.0	0.0	13.18324854837881	0.0
30	8.221571347823852E-5	0.0	0.0	13.440378192282	0.0
31	1.6443142695647705E-4	0.0	0.0	13.661291814398027	0.0
32	1.6443142695647705E-4	0.0	0.0	13.871846256615795	0.0
33	1.6443142695647705E-4	0.0	0.0	14.08400390524639	0.0
34	1.6443142695647705E-4	0.0	0.0	14.305123066646113	0.0
35	1.6443142695647705E-4	0.0	0.0	14.551975746364525	0.0
36	1.6443142695647705E-4	0.0	0.0	14.77338266276142	0.0
37	1.6443142695647705E-4	0.0	0.0	15.000996865525924	0.0
38	1.6443142695647705E-4	0.0	0.0	15.232927393248035	0.0
39	1.6443142695647705E-4	0.0	0.0	15.500539540619702	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTAA	40	6.8212103E-9	45.0	28
TACCTCG	20	7.034843E-4	45.0	29
CGTAAAT	35	1.2126839E-7	45.0	16
TAAATCG	20	7.034843E-4	45.0	35
GCGTACT	20	7.034843E-4	45.0	18
CTTACCG	20	7.034843E-4	45.0	1
CGGTCGA	30	2.1665055E-6	44.999996	40
CGTTTTT	15625	0.0	43.632	1
CGCATCG	320	0.0	43.59375	21
CGGTCTA	1070	0.0	42.897194	31
ACGGGTA	170	0.0	42.35294	5
CCGCATC	335	0.0	42.313435	20
TGTTACG	70	0.0	41.785713	1
CGACGGT	1105	0.0	41.742085	28
ACGGTCT	1100	0.0	41.727272	30
ATAACGC	365	0.0	41.301373	11
TCACGAC	1155	0.0	41.298702	25
TAGCACG	60	3.6379788E-12	41.249996	1
CGTTTCG	60	3.6379788E-12	41.249996	42
TGCGTAG	205	0.0	40.609756	1
>>END_MODULE