FastQCFastQC Report
Sat 18 Jun 2016
SRR3549264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2266988
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421791.860574471501393No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG502.1827873E-1145.0000041
ACCGTAT207.034741E-445.027
CGTTTTT209400.043.2915461
ATAGCGG4300.041.8604662
CGTAAGG2650.040.754722
TACGGGT2550.039.7058834
CGTTGAC403.460209E-739.37529
AGGGCGA14500.039.258626
TCGTCCC10100.039.20792438
GGTACCT8250.039.08
TAGCGCG1500.039.01
AATACGG2850.038.684212
GTCGATA356.250979E-638.57142626
CGCGATT356.250979E-638.57142613
CCGTATC356.250979E-638.57142628
GTTTTTT242450.038.253252
GTAGGGC9300.038.2258074
TAGGGAC16200.038.1944435
TAGCGGG11850.038.1645553
CGACGTC659.094947E-1238.0769218
Produced by FastQC (version 0.11.5)