##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549264_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2266988
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.346699232638198	31.0	31.0	33.0	30.0	34.0
2	31.75443892953999	31.0	31.0	34.0	30.0	34.0
3	31.757509523649883	31.0	31.0	34.0	30.0	34.0
4	35.603080827953214	37.0	35.0	37.0	33.0	37.0
5	35.53555951773895	37.0	35.0	37.0	33.0	37.0
6	35.58296426800671	37.0	35.0	37.0	33.0	37.0
7	35.95177742449453	37.0	35.0	37.0	35.0	37.0
8	35.934309753734915	37.0	35.0	37.0	35.0	37.0
9	37.66853596049031	39.0	37.0	39.0	35.0	39.0
10	36.982944329656796	39.0	37.0	39.0	32.0	39.0
11	36.71142635073498	39.0	35.0	39.0	32.0	39.0
12	36.36266491044505	38.0	35.0	39.0	32.0	39.0
13	36.203300149802295	38.0	35.0	39.0	32.0	39.0
14	37.35643682277983	39.0	35.0	41.0	32.0	41.0
15	37.49566164443746	39.0	35.0	41.0	32.0	41.0
16	37.550827794412676	39.0	35.0	41.0	32.0	41.0
17	37.46773825004808	39.0	35.0	41.0	32.0	41.0
18	37.28649026814434	39.0	36.0	41.0	32.0	41.0
19	37.143974736522644	38.0	35.0	41.0	32.0	41.0
20	36.91762109018663	38.0	35.0	41.0	32.0	41.0
21	36.79927419112938	38.0	35.0	41.0	31.0	41.0
22	36.71045501784747	38.0	35.0	40.0	31.0	41.0
23	36.61307161749423	38.0	35.0	40.0	31.0	41.0
24	36.45041570577347	38.0	35.0	40.0	31.0	41.0
25	36.3385571516038	38.0	35.0	40.0	31.0	41.0
26	36.18114123233118	38.0	35.0	40.0	30.0	41.0
27	36.089774626067715	38.0	35.0	40.0	30.0	41.0
28	36.112680790546754	38.0	35.0	40.0	30.0	41.0
29	36.098250630351814	38.0	35.0	40.0	30.0	41.0
30	36.01374511025202	38.0	35.0	40.0	30.0	41.0
31	35.862114841366605	38.0	35.0	40.0	30.0	41.0
32	35.63671400113278	38.0	34.0	40.0	29.0	41.0
33	35.296967165243046	38.0	34.0	40.0	27.0	41.0
34	34.91232154735711	38.0	34.0	40.0	24.0	41.0
35	34.61256962983483	38.0	34.0	40.0	23.0	41.0
36	34.398722445817974	37.0	33.0	40.0	22.0	41.0
37	34.26549324478118	37.0	33.0	40.0	21.0	41.0
38	34.1866150151655	37.0	33.0	40.0	21.0	41.0
39	34.11861509633046	37.0	33.0	40.0	21.0	41.0
40	33.95570730855214	37.0	33.0	40.0	20.0	41.0
41	33.87810125152846	37.0	33.0	40.0	20.0	41.0
42	33.79553839720369	37.0	33.0	40.0	20.0	41.0
43	33.71875501767102	36.0	33.0	40.0	19.0	41.0
44	33.574463561342185	36.0	33.0	40.0	18.0	41.0
45	33.49750594180472	36.0	33.0	40.0	19.0	41.0
46	33.40334355541361	36.0	33.0	40.0	18.0	41.0
47	33.266381207134756	36.0	32.0	40.0	18.0	41.0
48	33.17963085821363	35.0	32.0	40.0	18.0	41.0
49	33.04931389138363	35.0	32.0	40.0	18.0	41.0
50	32.9713769989078	35.0	32.0	40.0	18.0	41.0
51	32.54622829939991	35.0	31.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	2.0
12	8.0
13	9.0
14	34.0
15	72.0
16	225.0
17	538.0
18	1255.0
19	2491.0
20	4372.0
21	6961.0
22	10709.0
23	16086.0
24	24264.0
25	35958.0
26	49967.0
27	56086.0
28	55622.0
29	57034.0
30	62044.0
31	73333.0
32	88244.0
33	111800.0
34	173954.0
35	235849.0
36	195857.0
37	219867.0
38	305563.0
39	478628.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.273914109823252	23.82337268657796	27.06017852763226	18.842534675966522
2	34.5487492655453	25.136701208828633	27.63270030542729	12.681849220198785
3	26.920477744037463	24.567752453916828	36.04443428902138	12.46733551302433
4	23.586362168657267	27.451843591584957	35.28664465802201	13.675149581735765
5	21.747358168636094	31.096062264114323	34.262069318408386	12.894510248841193
6	20.211090663029534	39.62685289908901	30.610175263389134	9.55188117449232
7	84.07834536398076	3.2700217204502184	11.419866360121889	1.2317665554471395
8	84.50494665168056	2.891854742945265	11.036141347020804	1.5670572583533746
9	80.2432566912573	5.294778799005552	12.272363153223573	2.1896013565135766
10	39.78980038712159	28.58780020008928	20.248056010883165	11.374343401905964
11	30.874314288386174	25.68791718350516	27.422156623678646	16.01561190443002
12	30.148461306367746	21.977354974971195	30.44440464616487	17.429779072496192
13	21.75587166760477	28.571302538875372	31.63510349415171	18.03772229936815
14	17.860262162834566	30.765667925899916	32.30661123923021	19.06745867203532
15	17.83688312421592	27.587794906722046	37.562969014392664	17.012352954669367
16	19.613778282020018	25.01102784840502	37.751456999331275	17.62373687024369
17	19.638833553596225	26.61796180659095	31.737486038743917	22.005718601068907
18	20.36600987742326	27.659034807418475	33.5180865536121	18.456868761546158
19	23.928401914787376	29.674440270526354	28.598916271281542	17.798241543404732
20	25.031539646438354	27.260267809092948	30.846391776224664	16.86180076824403
21	22.946835183953336	28.464905857463734	31.207884646941224	17.380374311641702
22	23.126677335742404	26.533664933382973	30.063546873649088	20.276110857225536
23	20.858557698585084	29.036457184599122	30.344668785189867	19.76031633162593
24	20.564687594288102	27.06128131247276	34.84001679761869	17.534014295620445
25	19.918632123328397	28.825251831946176	32.0759527619908	19.180163282734625
26	18.923699640227472	31.822444582856196	30.23258173400124	19.021274042915092
27	18.95400416764447	32.373925225894446	30.26094536009895	18.411125246362133
28	17.96211537070333	30.883842349408113	33.12183390472292	18.032208375165638
29	18.5791014332674	28.08091617600093	33.29435356517105	20.045628825560613
30	19.245933370622165	30.47788519392251	31.94110423169421	18.335077203761113
31	22.27338653755556	29.58529114402017	29.439855879254768	18.701466439169508
32	23.05892223514196	29.222827822643964	29.701965780145283	18.01628416206879
33	22.21948241455182	30.48507535108258	28.24545167420383	19.049990560161763
34	19.960096833331274	30.449786236186515	29.462529135575487	20.127587794906724
35	20.301783688312423	29.575410191849272	29.965002020301828	20.157804099536477
36	22.702061060755504	31.858660037018282	26.579099668811658	18.860179233414556
37	20.07169865919008	33.476136618279405	28.570773202151926	17.881391520378582
38	21.04303154670426	32.274851035823744	27.134418003094858	19.54769941437714
39	20.09516592059596	32.81905329891469	27.188498571673076	19.89728220881628
40	21.873340308815045	30.72645289697166	28.187621637167908	19.212585157045385
41	18.156602505174266	29.78440997482122	29.88383705604088	22.17515046396364
42	19.88691603131556	30.62552602836892	27.13993192729737	22.347626013018154
43	20.507431005369238	30.372988299893954	27.826790437355648	21.29279025738116
44	19.20455688340653	31.729545987892305	27.836362609771204	21.229534518929963
45	18.936536055770915	33.77635876325768	26.367497313616127	20.919607867355275
46	20.009369260004906	33.1661658553111	26.40075730440567	20.423707580278325
47	20.160936008483503	30.47784108252889	28.602180514409426	20.75904239457818
48	20.768482232812875	29.227150739218732	29.892615223371276	20.111751804597112
49	20.309547293589556	30.22274489322396	28.635661062167067	20.832046751019416
50	19.27076808523027	31.39465228752865	29.17580507704496	20.158774550196117
51	18.04261866405998	31.938545770864245	27.990223150718048	22.028612414357728
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2209.0
1	2898.5
2	3588.0
3	31795.0
4	60002.0
5	40279.0
6	20556.0
7	21140.5
8	21725.0
9	24038.5
10	26352.0
11	28707.5
12	31063.0
13	31197.5
14	31332.0
15	29715.5
16	28099.0
17	26114.5
18	24130.0
19	22534.0
20	20938.0
21	20041.0
22	19144.0
23	19777.5
24	20411.0
25	21850.5
26	26131.0
27	28972.0
28	33993.0
29	39014.0
30	42000.5
31	44987.0
32	53774.0
33	62561.0
34	66555.0
35	70549.0
36	74412.5
37	78276.0
38	86146.5
39	94017.0
40	107115.5
41	120214.0
42	130594.5
43	140975.0
44	148890.5
45	156806.0
46	156936.5
47	157067.0
48	154449.0
49	151831.0
50	143060.5
51	134290.0
52	122831.0
53	111372.0
54	106744.0
55	102116.0
56	100428.5
57	98741.0
58	94262.0
59	89783.0
60	83051.5
61	76320.0
62	67338.5
63	58357.0
64	49452.5
65	40548.0
66	33188.5
67	25829.0
68	22141.5
69	18454.0
70	15104.5
71	11755.0
72	10124.0
73	8493.0
74	6912.0
75	4466.0
76	3601.0
77	2836.0
78	2071.0
79	1475.0
80	879.0
81	735.5
82	592.0
83	410.0
84	228.0
85	142.0
86	56.0
87	45.5
88	35.0
89	27.0
90	19.0
91	12.0
92	5.0
93	5.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2266988.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.15366097686528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3002569195447	19.153915210129973
2	7.018136045677324	3.39027357473616
3	2.816293626511617	2.0407140439820415
4	1.6588039369001217	1.6026475167589989
5	1.0607030927972063	1.28099314502681
6	0.7637826324067103	1.1068888057901638
7	0.6049824104252043	1.0228778026864005
8	0.4747860853559861	0.917425771377721
9	0.38037705526450993	0.826874859261359
>10	3.9480886136251025	22.20950555337454
>50	1.330302571877111	23.07585200120445
>100	0.6386793583839939	20.62931047421928
>500	0.0038831029169004065	0.6026304789271196
>1k	7.396386508381727E-4	0.25627315556835484
>5k	0.0	0.0
>10k+	1.8490966270954317E-4	1.8838176069567165
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42179	1.860574471501393	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.411139362008092E-5	0.0	0.0	0.031892537587318505	0.0
2	4.411139362008092E-5	0.0	0.0	0.08932557208066386	0.0
3	4.411139362008092E-5	0.0	0.0	0.1400536747437569	0.0
4	4.411139362008092E-5	0.0	0.0	0.2511261638791207	0.0
5	4.411139362008092E-5	0.0	0.0	0.44001115136030716	0.0
6	4.411139362008092E-5	0.0	0.0	0.8528055728570244	0.0
7	4.411139362008092E-5	0.0	0.0	1.0730537612020885	0.0
8	4.411139362008092E-5	0.0	0.0	1.4371492041422362	0.0
9	4.411139362008092E-5	0.0	0.0	1.625637189080842	0.0
10	4.411139362008092E-5	0.0	0.0	1.861677256341895	0.0
11	4.411139362008092E-5	0.0	0.0	2.108921617582449	0.0
12	4.411139362008092E-5	0.0	0.0	2.2945864733293693	0.0
13	4.411139362008092E-5	0.0	0.0	2.40393861811355	0.0
14	4.411139362008092E-5	0.0	0.0	2.454093272659582	0.0
15	4.411139362008092E-5	0.0	0.0	2.5064093854929976	4.411139362008092E-5
16	4.411139362008092E-5	0.0	0.0	2.5962642942971024	4.411139362008092E-5
17	4.411139362008092E-5	0.0	0.0	2.7031903124321786	4.411139362008092E-5
18	4.411139362008092E-5	0.0	0.0	2.8295253437600905	4.411139362008092E-5
19	4.411139362008092E-5	0.0	0.0	2.9102050826912187	8.822278724016184E-5
20	4.411139362008092E-5	0.0	0.0	2.995428295165215	8.822278724016184E-5
21	4.411139362008092E-5	0.0	0.0	3.116028845322516	8.822278724016184E-5
22	4.411139362008092E-5	0.0	0.0	3.2407317550864847	8.822278724016184E-5
23	4.411139362008092E-5	0.0	0.0	3.392298503565083	8.822278724016184E-5
24	4.411139362008092E-5	0.0	0.0	3.5132519448713446	8.822278724016184E-5
25	4.411139362008092E-5	0.0	0.0	3.6207072997298617	8.822278724016184E-5
26	4.411139362008092E-5	0.0	0.0	3.722207616449668	8.822278724016184E-5
27	4.411139362008092E-5	0.0	0.0	3.8269721762973603	8.822278724016184E-5
28	4.411139362008092E-5	0.0	0.0	3.9394562300285667	8.822278724016184E-5
29	4.411139362008092E-5	0.0	0.0	4.057410096568663	8.822278724016184E-5
30	4.411139362008092E-5	0.0	0.0	4.197110880163459	8.822278724016184E-5
31	4.411139362008092E-5	0.0	0.0	4.325519146991515	8.822278724016184E-5
32	4.411139362008092E-5	0.0	0.0	4.452736406191828	8.822278724016184E-5
33	4.411139362008092E-5	0.0	0.0	4.577527538743037	8.822278724016184E-5
34	4.411139362008092E-5	0.0	0.0	4.709111825911738	8.822278724016184E-5
35	4.411139362008092E-5	0.0	0.0	4.850312396889618	8.822278724016184E-5
36	4.411139362008092E-5	0.0	0.0	4.980441008068856	8.822278724016184E-5
37	4.411139362008092E-5	0.0	0.0	5.123406034791539	8.822278724016184E-5
38	4.411139362008092E-5	0.0	0.0	5.28260405436641	8.822278724016184E-5
39	4.411139362008092E-5	0.0	0.0	5.443831198047806	8.822278724016184E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	50	2.1827873E-11	45.000004	1
ACCGTAT	20	7.034741E-4	45.0	27
CGTTTTT	20940	0.0	43.291546	1
ATAGCGG	430	0.0	41.860466	2
CGTAAGG	265	0.0	40.75472	2
TACGGGT	255	0.0	39.705883	4
CGTTGAC	40	3.460209E-7	39.375	29
AGGGCGA	1450	0.0	39.25862	6
TCGTCCC	1010	0.0	39.207924	38
GGTACCT	825	0.0	39.0	8
TAGCGCG	150	0.0	39.0	1
AATACGG	285	0.0	38.68421	2
GTCGATA	35	6.250979E-6	38.571426	26
CGCGATT	35	6.250979E-6	38.571426	13
CCGTATC	35	6.250979E-6	38.571426	28
GTTTTTT	24245	0.0	38.25325	2
GTAGGGC	930	0.0	38.225807	4
TAGGGAC	1620	0.0	38.194443	5
TAGCGGG	1185	0.0	38.164555	3
CGACGTC	65	9.094947E-12	38.07692	18
>>END_MODULE