FastQCFastQC Report
Sat 18 Jun 2016
SRR3549261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740621
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225960.8244846697153674No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC54330.19823974201467476No Hit
GAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT32990.12037417796915371RNA PCR Primer, Index 35 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG302.1666056E-645.00000430
CGAGTTA207.034998E-445.032
ATCGTAC253.8922248E-545.040
CTAACGG950.045.02
CCATACG351.2127566E-745.028
CGTACTC207.034998E-445.042
TAATGCG1150.044.9999961
GACGTAG1050.042.8571431
TACGCGG2950.041.9491542
TACGGGA7050.041.8085144
TCGAGTA603.6379788E-1241.25000440
TAGGGCG7450.041.0738265
CGCATGG2250.041.02
CGTTTTT126250.040.99011
CGAATCC451.9299478E-840.041
TGGGCGA13350.039.775286
TCGTCCC8000.039.6562538
CGAATGT403.4605546E-739.37519
ATCGAAC800.039.37521
AGGGTAC7450.039.2617456
Produced by FastQC (version 0.11.5)