##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549261_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2740621
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266136397553694	31.0	31.0	33.0	30.0	34.0
2	31.65767831451339	31.0	31.0	34.0	30.0	34.0
3	31.74401057278624	31.0	31.0	34.0	30.0	34.0
4	35.51996500063307	37.0	35.0	37.0	33.0	37.0
5	35.41027453266979	37.0	35.0	37.0	33.0	37.0
6	35.44740918207954	37.0	35.0	37.0	33.0	37.0
7	35.86716076392905	37.0	35.0	37.0	35.0	37.0
8	35.86040499580205	37.0	35.0	37.0	35.0	37.0
9	37.583319619896365	39.0	37.0	39.0	35.0	39.0
10	36.918025148314925	39.0	37.0	39.0	32.0	39.0
11	36.58677686553522	39.0	35.0	39.0	32.0	39.0
12	35.871819926943566	37.0	35.0	39.0	31.0	39.0
13	35.43242389224924	37.0	35.0	39.0	30.0	39.0
14	36.484063283467506	38.0	35.0	41.0	31.0	41.0
15	36.78577410010359	38.0	35.0	41.0	31.0	41.0
16	36.971350653738696	38.0	35.0	41.0	32.0	41.0
17	36.90668136893062	38.0	35.0	41.0	32.0	41.0
18	36.79171837331758	38.0	35.0	40.0	31.0	41.0
19	36.693406348415195	38.0	35.0	40.0	31.0	41.0
20	36.50715403552699	38.0	35.0	40.0	31.0	41.0
21	36.348238592640136	38.0	35.0	40.0	31.0	41.0
22	36.227600240967284	38.0	35.0	40.0	30.0	41.0
23	36.16086244686879	38.0	35.0	40.0	30.0	41.0
24	36.04024270411706	37.0	34.0	40.0	30.0	41.0
25	35.94208429403409	37.0	34.0	40.0	30.0	41.0
26	35.66671677696405	37.0	34.0	40.0	30.0	41.0
27	35.6391456534851	37.0	34.0	40.0	30.0	41.0
28	35.7178321993446	37.0	34.0	40.0	30.0	41.0
29	35.7606031625679	37.0	34.0	40.0	30.0	41.0
30	35.777852172919935	37.0	35.0	40.0	30.0	41.0
31	35.63876508280423	37.0	34.0	40.0	30.0	41.0
32	35.42635774884597	37.0	34.0	40.0	29.0	41.0
33	35.231990121946815	37.0	34.0	40.0	27.0	41.0
34	35.0392582557019	37.0	34.0	40.0	27.0	41.0
35	34.93329212612762	37.0	34.0	40.0	26.0	41.0
36	34.65882185096006	37.0	34.0	40.0	24.0	41.0
37	34.5954756239553	37.0	33.0	40.0	24.0	41.0
38	34.54610579135167	36.0	33.0	40.0	24.0	41.0
39	34.42774356614797	36.0	33.0	40.0	24.0	41.0
40	34.25771166461908	36.0	33.0	40.0	23.0	41.0
41	34.257390934390415	36.0	33.0	40.0	23.0	41.0
42	34.16385702364537	36.0	33.0	40.0	23.0	41.0
43	34.04240790682112	36.0	33.0	40.0	23.0	41.0
44	33.949768683812906	35.0	33.0	40.0	23.0	41.0
45	33.792314953435735	35.0	33.0	40.0	23.0	41.0
46	33.724020577817946	35.0	33.0	40.0	23.0	41.0
47	33.697779079996835	35.0	33.0	40.0	23.0	41.0
48	33.621718946180444	35.0	33.0	40.0	23.0	41.0
49	33.5196201882712	35.0	33.0	39.0	22.0	41.0
50	33.346208395834374	35.0	32.0	39.0	21.0	41.0
51	32.84478919193862	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	6.0
13	15.0
14	35.0
15	131.0
16	316.0
17	740.0
18	1666.0
19	3223.0
20	5276.0
21	8531.0
22	12615.0
23	18292.0
24	26026.0
25	36586.0
26	49588.0
27	59546.0
28	65500.0
29	72945.0
30	82840.0
31	98430.0
32	119825.0
33	152271.0
34	247284.0
35	353740.0
36	202537.0
37	241716.0
38	341209.0
39	539514.0
40	215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.660845844792114	23.6645636153266	28.696342909143585	14.978247630737703
2	30.924706480757465	26.07635276822297	29.444129633393306	13.554811117626262
3	29.021743612122947	25.64772728516639	32.04565680551962	13.284872297191038
4	25.259567083518665	27.98737950267476	31.715512652059513	15.037540761747065
5	22.66964312102987	32.04945156590423	30.813563787185462	14.467341525880448
6	22.345118131985416	40.96564245840633	26.712631918094477	9.976607491513786
7	86.92004476357731	3.7049997062709514	7.857708161763338	1.5172473683884053
8	86.65178439485067	3.221569126121415	7.516106750988189	2.6105397280397398
9	81.64448860313045	6.049723766985657	9.523644458682904	2.7821431712009796
10	45.71617162679553	30.223879916267155	13.508434767156787	10.551513689780528
11	40.28605195683752	22.737510950985197	23.299244952147706	13.677192140029575
12	38.767928874514205	19.923477197321336	25.865524638394	15.443069289770456
13	22.19369259740767	34.635763208411525	26.266857037145964	16.903687157034845
14	16.785538751983584	36.859857674592725	28.340620611168056	18.013982962255636
15	15.319776065351611	25.572269934441866	43.454056580607094	15.653897419599428
16	17.54365160304909	21.566462491530206	43.14737426298638	17.742511642434327
17	17.949180131072485	22.127795123805882	28.980840473746643	30.94218427137499
18	20.348891729283253	24.438183900656092	33.86199697075954	21.350927399301106
19	27.387807361908123	26.581493756342084	26.075294613884953	19.955404267864836
20	30.667939857426475	23.694337889113452	27.262142412248902	18.37557984121117
21	23.37265897035745	28.300191817839824	28.25534796675644	20.071801245046288
22	23.325333929791825	25.280912610682034	26.72065929583113	24.673094163695016
23	21.8993432510369	29.911323017666437	26.42645590178284	21.762877829513823
24	21.531287981811424	24.109718198904556	36.15315652912241	18.205837290161607
25	19.914902498375366	24.88979687450399	33.58691333095674	21.608387296163897
26	18.783188189829968	33.58523487924817	27.582215855457576	20.049361075464283
27	19.805182839947587	32.60618670002164	28.25410737201532	19.334523088015455
28	17.0105972332548	29.71665910755263	34.665428017956515	18.607315641236056
29	17.298707117839353	25.5395401261247	34.74303816543769	22.418714590598263
30	19.3168263689142	29.496490029084647	30.129704180183982	21.05697942181717
31	25.223699300268077	26.81859330421828	26.947432716891534	21.010274678622107
32	27.69116926419231	26.278350782541622	27.786257202291015	18.24422275097505
33	25.384027926517383	27.177891434094683	27.670480522480123	19.76760011690781
34	19.48313904038537	26.92583177316382	29.27664934334226	24.31437984310855
35	19.94080903561638	24.620733768003674	31.2020888696394	24.236368326740546
36	26.464257553306346	25.924416400516527	27.323296435369944	20.288029610807186
37	20.396143793687635	30.440144770108674	29.368818234991267	19.794893201212428
38	20.164845850630204	30.426169835230777	26.637612424337405	22.771371889801618
39	21.149002361143697	29.230127040550297	28.00255854421315	21.61831205409285
40	23.977120513927318	26.21387634408406	27.396199620450982	22.412803521537636
41	16.944480831169287	25.32411449813747	30.123172813752795	27.608231856940453
42	21.352131505961605	26.37048318611001	26.471445705188714	25.80593960273967
43	21.552742973216652	26.225698482205313	27.462826855665195	24.758731688912842
44	20.28843097969402	28.588119262021273	28.442349379939802	22.681100378344908
45	19.035868148131392	32.21269923860322	24.378562376921142	24.372870236344244
46	21.088030778425765	31.072483207273095	26.15385345146228	21.68563256283886
47	20.877494553241764	26.779441593711788	28.660292685489896	23.68277116755655
48	21.670417033219845	24.13642747391923	31.35008452463876	22.843070968222165
49	20.591646929655724	24.812405655506545	31.330490425345204	23.265456989492527
50	19.713232876782307	30.01648166601657	28.0179930023159	22.252292454885225
51	17.905905267455807	30.183852491825757	26.71186566840143	25.198376572317006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1552.0
1	2941.5
2	4331.0
3	19513.5
4	34696.0
5	24120.5
6	13545.0
7	13857.0
8	14169.0
9	15295.0
10	16421.0
11	16928.0
12	17435.0
13	17405.0
14	17375.0
15	16692.0
16	16009.0
17	15342.5
18	14676.0
19	14252.0
20	13828.0
21	13643.5
22	13459.0
23	13478.0
24	13497.0
25	16127.0
26	22096.5
27	25436.0
28	29813.0
29	34190.0
30	37581.0
31	40972.0
32	48559.5
33	56147.0
34	62579.0
35	69011.0
36	74032.0
37	79053.0
38	93926.5
39	108800.0
40	137118.5
41	165437.0
42	199089.0
43	232741.0
44	237557.0
45	242373.0
46	238244.0
47	234115.0
48	228037.0
49	221959.0
50	207427.5
51	192896.0
52	172971.5
53	153047.0
54	141965.0
55	130883.0
56	125509.5
57	120136.0
58	114985.5
59	109835.0
60	107030.0
61	104225.0
62	90354.5
63	76484.0
64	62603.5
65	48723.0
66	39065.5
67	29408.0
68	24289.5
69	19171.0
70	15897.0
71	12623.0
72	10757.0
73	8891.0
74	7702.0
75	4835.5
76	3158.0
77	2709.5
78	2261.0
79	1739.5
80	1218.0
81	957.5
82	697.0
83	470.5
84	244.0
85	195.0
86	146.0
87	95.5
88	45.0
89	29.5
90	14.0
91	12.5
92	11.0
93	8.5
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2740621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.517939845573135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.63784798025583	15.934026060006776
2	7.994754140589179	3.120822607923327
3	2.6701279685281656	1.5634619120914541
4	1.2541535733154336	0.9791397600432494
5	0.7129212686463235	0.6957377218034313
6	0.4726740703635276	0.553537444315052
7	0.3384831493192839	0.4624545623007753
8	0.24581778634499557	0.38382854134828587
9	0.18452388852803842	0.3241373540725347
>10	1.9978209051337688	9.58555583353198
>50	1.0351436890232129	14.773927502051187
>100	1.4438272342669403	48.441084085957456
>500	0.008503104061009957	1.1095922291621523
>1k	0.003023325888359096	1.0389657211901546
>5k	1.889578680224435E-4	0.2003727508155944
>10k+	1.889578680224435E-4	0.8333559133865582
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22596	0.8244846697153674	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5433	0.19823974201467476	No Hit
GAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	3299	0.12037417796915371	RNA PCR Primer, Index 35 (95% over 21bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.297616124228779E-5	0.0	0.0	0.0977515679840445	0.0
2	7.297616124228779E-5	0.0	0.0	0.37243383890001575	0.0
3	7.297616124228779E-5	0.0	0.0	0.5075856895207327	0.0
4	7.297616124228779E-5	0.0	0.0	0.9329637334020282	0.0
5	7.297616124228779E-5	0.0	0.0	1.4743373855779403	0.0
6	1.0946424186343169E-4	0.0	0.0	2.118716889347341	0.0
7	1.0946424186343169E-4	0.0	0.0	2.3911368992648017	0.0
8	1.0946424186343169E-4	0.0	0.0	3.061641868758942	0.0
9	1.0946424186343169E-4	0.0	0.0	3.227005850133966	0.0
10	1.0946424186343169E-4	0.0	0.0	3.5726574378580622	0.0
11	1.0946424186343169E-4	0.0	0.0	4.038135882341995	0.0
12	1.0946424186343169E-4	0.0	0.0	4.394989310816782	0.0
13	1.0946424186343169E-4	0.0	0.0	4.542583596929309	0.0
14	1.0946424186343169E-4	0.0	0.0	4.601730775616184	0.0
15	1.0946424186343169E-4	0.0	0.0	4.694738893119479	0.0
16	1.0946424186343169E-4	0.0	0.0	4.885133697800608	0.0
17	1.0946424186343169E-4	0.0	0.0	5.106981227977163	0.0
18	1.0946424186343169E-4	0.0	0.0	5.37100897935176	0.0
19	1.0946424186343169E-4	0.0	0.0	5.5128016606455255	0.0
20	1.0946424186343169E-4	0.0	0.0	5.655761960519167	0.0
21	1.0946424186343169E-4	0.0	0.0	5.868852351346647	0.0
22	1.0946424186343169E-4	0.0	0.0	6.08840113244407	0.0
23	1.0946424186343169E-4	0.0	0.0	6.328529191011818	0.0
24	1.0946424186343169E-4	0.0	0.0	6.5094370947314495	0.0
25	1.0946424186343169E-4	0.0	0.0	6.655608345699752	0.0
26	1.0946424186343169E-4	0.0	0.0	6.810755664500856	0.0
27	1.0946424186343169E-4	0.0	0.0	6.940142398383432	0.0
28	1.0946424186343169E-4	0.0	0.0	7.085620375819933	0.0
29	1.0946424186343169E-4	0.0	0.0	7.235075554044138	0.0
30	1.0946424186343169E-4	0.0	0.0	7.425433870644646	0.0
31	1.0946424186343169E-4	0.0	0.0	7.6115230818124795	0.0
32	1.0946424186343169E-4	0.0	0.0	7.778273610251107	0.0
33	1.0946424186343169E-4	0.0	0.0	7.932946584004136	0.0
34	1.0946424186343169E-4	0.0	0.0	8.097617291847358	0.0
35	1.0946424186343169E-4	0.0	0.0	8.304833101694834	0.0
36	1.0946424186343169E-4	0.0	0.0	8.502160641693981	0.0
37	1.0946424186343169E-4	0.0	0.0	8.693394672229395	0.0
38	1.0946424186343169E-4	0.0	0.0	8.8658008531643	0.0
39	1.0946424186343169E-4	0.0	0.0	9.062435119631646	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	30	2.1666056E-6	45.000004	30
CGAGTTA	20	7.034998E-4	45.0	32
ATCGTAC	25	3.8922248E-5	45.0	40
CTAACGG	95	0.0	45.0	2
CCATACG	35	1.2127566E-7	45.0	28
CGTACTC	20	7.034998E-4	45.0	42
TAATGCG	115	0.0	44.999996	1
GACGTAG	105	0.0	42.857143	1
TACGCGG	295	0.0	41.949154	2
TACGGGA	705	0.0	41.808514	4
TCGAGTA	60	3.6379788E-12	41.250004	40
TAGGGCG	745	0.0	41.073826	5
CGCATGG	225	0.0	41.0	2
CGTTTTT	12625	0.0	40.9901	1
CGAATCC	45	1.9299478E-8	40.0	41
TGGGCGA	1335	0.0	39.77528	6
TCGTCCC	800	0.0	39.65625	38
CGAATGT	40	3.4605546E-7	39.375	19
ATCGAAC	80	0.0	39.375	21
AGGGTAC	745	0.0	39.261745	6
>>END_MODULE