FastQCFastQC Report
Sat 18 Jun 2016
SRR3549260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186712
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339481.5524678146916466No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30500.1394788156830895No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT21940.10033328577334372No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGT253.8919643E-545.00000431
CACCGTA253.8919643E-545.00000434
CGGTAAC253.8919643E-545.00000432
ACCGGGT207.034686E-445.04
CGTATCG207.034686E-445.028
CGCATCG406.8193913E-945.021
CACGACT207.034686E-445.014
GTTCGGA207.034686E-445.035
CACGAAT302.1664055E-644.99999638
CGTTTTT143050.043.1597331
CGTATGG2150.041.8604662
TGTTACG603.6379788E-1241.2499961
ACGGGAT13600.040.698535
TCGTAAG1000.040.5000041
GGCGATA7800.040.3846178
TGGGATC14950.040.334456
GTGTACG950.040.2631571
CTCACGA1400.040.1785724
TTTGACG1400.040.178571
AGGGCGA19050.040.039376