##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549260_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2186712
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.348828743794336	31.0	31.0	33.0	30.0	34.0
2	31.739936031813976	31.0	31.0	34.0	30.0	34.0
3	31.785835080248336	31.0	31.0	34.0	30.0	34.0
4	35.583403758702566	37.0	35.0	37.0	33.0	37.0
5	35.49152654762035	37.0	35.0	37.0	33.0	37.0
6	35.53787695864842	37.0	35.0	37.0	33.0	37.0
7	35.958947954737525	37.0	35.0	37.0	35.0	37.0
8	35.9687439406744	37.0	35.0	37.0	35.0	37.0
9	37.74185672370207	39.0	38.0	39.0	35.0	39.0
10	37.046860308993594	39.0	37.0	39.0	33.0	39.0
11	36.68266420086413	39.0	35.0	39.0	32.0	39.0
12	36.1299302331537	38.0	35.0	39.0	32.0	39.0
13	35.811202847014144	38.0	35.0	39.0	31.0	39.0
14	36.90347837300934	39.0	35.0	41.0	31.0	41.0
15	37.163157745510155	39.0	35.0	41.0	32.0	41.0
16	37.32078481299778	39.0	35.0	41.0	32.0	41.0
17	37.24271143159227	39.0	35.0	41.0	32.0	41.0
18	37.12249761285437	39.0	35.0	41.0	32.0	41.0
19	36.996417909628704	38.0	35.0	41.0	32.0	41.0
20	36.80402540435137	38.0	35.0	40.0	32.0	41.0
21	36.661407629354024	38.0	35.0	40.0	31.0	41.0
22	36.56564055989083	38.0	35.0	40.0	31.0	41.0
23	36.49309145420156	38.0	35.0	40.0	31.0	41.0
24	36.377885153600474	38.0	35.0	40.0	31.0	41.0
25	36.29510287591599	38.0	35.0	40.0	31.0	41.0
26	36.0960254482529	38.0	35.0	40.0	30.0	41.0
27	36.05771130354615	38.0	35.0	40.0	30.0	41.0
28	36.10045492959292	38.0	35.0	40.0	30.0	41.0
29	36.1377625402888	38.0	35.0	40.0	30.0	41.0
30	36.11138183720581	38.0	35.0	40.0	30.0	41.0
31	35.98551386739543	38.0	35.0	40.0	30.0	41.0
32	35.74943568243097	38.0	34.0	40.0	30.0	41.0
33	35.51184243741288	38.0	34.0	40.0	29.0	41.0
34	35.284614068976616	38.0	34.0	40.0	27.0	41.0
35	35.10521047124633	37.0	34.0	40.0	26.0	41.0
36	34.805404644050064	37.0	34.0	40.0	24.0	41.0
37	34.743721624063895	37.0	34.0	40.0	24.0	41.0
38	34.6865307365579	37.0	34.0	40.0	24.0	41.0
39	34.58574746011363	37.0	34.0	40.0	24.0	41.0
40	34.444290789093394	37.0	33.0	40.0	23.0	41.0
41	34.45435338535665	37.0	34.0	40.0	23.0	41.0
42	34.38023662924061	37.0	33.0	40.0	23.0	41.0
43	34.289568996740314	37.0	33.0	40.0	23.0	41.0
44	34.198879413475574	36.0	33.0	40.0	23.0	41.0
45	34.04770404150158	36.0	33.0	40.0	23.0	41.0
46	33.97559852417694	36.0	33.0	40.0	23.0	41.0
47	33.945978711416956	36.0	33.0	40.0	23.0	41.0
48	33.86202435437314	36.0	33.0	40.0	23.0	41.0
49	33.75573326528596	36.0	33.0	40.0	22.0	41.0
50	33.61452628421118	35.0	33.0	40.0	22.0	41.0
51	33.14117268300535	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	4.0
13	5.0
14	20.0
15	70.0
16	185.0
17	416.0
18	1023.0
19	1985.0
20	3505.0
21	5853.0
22	8940.0
23	13391.0
24	19669.0
25	28797.0
26	39338.0
27	45629.0
28	48072.0
29	52077.0
30	59828.0
31	71650.0
32	88091.0
33	113595.0
34	182872.0
35	267823.0
36	171267.0
37	201103.0
38	285906.0
39	475421.0
40	174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.39147359140115	24.156038838219207	29.082933646497573	16.369553923882066
2	31.166427037488248	26.0087290873238	29.938510421125414	12.886333454062537
3	27.02120809690531	25.571634490504465	34.479391890655926	12.927765521934301
4	23.305355254830083	28.258179403597733	34.273603474074314	14.16286186749787
5	21.699611105623422	32.47300055974449	32.1466658618053	13.680722472826783
6	20.82917183424246	40.20872433132484	28.84238985289329	10.119713981539407
7	87.82697492856856	2.8903669070275373	8.327068219317404	0.9555899450865043
8	88.35854927397847	2.4328763915870035	8.026114092756613	1.1824602416779164
9	84.36904356860894	5.048492897098475	9.020666644715902	1.5617968895766796
10	42.174689671067796	35.32499935976937	13.939924416201126	8.560386552961708
11	33.597108352631714	23.376283662411875	29.42582287928177	13.600785105674639
12	34.914565795587166	22.484853972539593	27.57706547547185	15.02351475640139
13	21.019091677367665	35.698711124281566	27.718190598487592	15.564006599863173
14	16.067547989858745	36.36199005630371	29.120844445907828	18.449617507929712
15	14.567167509941866	27.494155608969084	41.59797906628765	16.3406978148014
16	16.760048877035477	24.450636389245588	41.687840008194954	17.101474725523982
17	16.785155063858433	25.815516629533292	30.61596588851207	26.783362418096214
18	19.002365194867913	26.59047007562038	35.16823431709343	19.238930412418277
19	24.14236534120634	28.028290876896456	28.493647082926334	19.335696698970875
20	26.848528750013717	26.584845192233818	29.505074285045307	17.06155177270715
21	21.17837191180183	31.17685365059505	30.188337558855487	17.456436878747635
22	22.367874690402758	27.52763052473302	27.800231580564795	22.304263204299424
23	18.64136658142453	32.55293792689663	28.489028276243054	20.31666721543578
24	18.59901989836796	26.458948412045114	39.228211122452336	15.713820567134585
25	16.933597108352632	29.941345728198314	34.351528687819886	18.773528475629163
26	17.134035026103117	35.51706854857887	28.87563611486103	18.47326031045698
27	16.552476960843496	35.910215885768224	29.517467320799444	18.019839832588836
28	15.442408511043062	32.122199905611716	36.107223996575684	16.328167586769542
29	15.192672834831471	27.803112618396934	35.81349532997486	21.190719216796726
30	17.16417159644251	33.46640984272277	32.04880203703094	17.320616523803775
31	23.599175382949376	30.66430330102913	28.136992891610785	17.599528424410714
32	24.130886920637014	29.689826552376353	28.502793234774398	17.676493292212232
33	23.09215845525154	31.414973713959586	27.210259055604947	18.282608775183927
34	17.82301464481834	30.619807272288256	29.59745041871083	21.959727664182573
35	18.752080749545435	29.79354391433348	31.246684519955075	20.207690816166007
36	24.251021625161428	30.37034598063211	27.115093345625763	18.2635390485807
37	18.345717222935622	34.68261938472007	29.65026944563344	17.321393946710863
38	18.758300132802123	33.6616801846791	28.6382020128851	18.94181766963368
39	19.14788047077073	35.03191092379792	27.334966836053397	18.485241769377954
40	23.371253278895438	30.22441912789613	27.060582280611257	19.34374531259718
41	16.7091962727602	28.98397228350144	30.224281935618407	24.082549508119953
42	19.257451369910626	29.302121175536605	26.98960814227022	24.45081931228255
43	19.358059040239407	31.027908567749208	27.578574590526785	22.035457801484604
44	18.6424641196463	32.263325028627456	28.581633063704775	20.512577788021467
45	17.0089156688215	36.088611577564855	25.58471348764721	21.317759265966437
46	19.43086240895006	35.32774320532379	26.143726288601332	19.097668097124814
47	19.14984689341806	31.499255503239564	28.364091841998395	20.98680576134397
48	19.89324611562931	29.300886445037115	31.01432653225482	19.791540907078755
49	18.803024815339196	30.38443105447814	30.264936580583086	20.54760754959958
50	18.14431895924109	34.27625585811026	28.852496350685414	18.726928831963242
51	16.723830115717114	34.342153882175616	26.986224066086432	21.947791936020838
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1494.0
1	2086.5
2	2679.0
3	23644.5
4	44610.0
5	29190.5
6	13771.0
7	14183.0
8	14595.0
9	16113.0
10	17631.0
11	19237.5
12	20844.0
13	21369.0
14	21894.0
15	21303.0
16	20712.0
17	20110.5
18	19509.0
19	18755.5
20	18002.0
21	17593.0
22	17184.0
23	16696.5
24	16209.0
25	18269.5
26	24717.5
27	29105.0
28	35108.0
29	41111.0
30	48066.5
31	55022.0
32	64121.5
33	73221.0
34	79378.0
35	85535.0
36	87910.5
37	90286.0
38	97716.5
39	105147.0
40	132626.5
41	160106.0
42	174082.0
43	188058.0
44	185519.0
45	182980.0
46	178648.5
47	174317.0
48	168154.5
49	161992.0
50	151398.5
51	140805.0
52	126604.0
53	112403.0
54	100237.0
55	88071.0
56	78079.5
57	68088.0
58	60875.0
59	53662.0
60	48863.0
61	44064.0
62	38568.5
63	33073.0
64	27298.0
65	21523.0
66	16336.5
67	11150.0
68	8747.0
69	6344.0
70	5388.0
71	4432.0
72	3535.5
73	2639.0
74	2267.5
75	1466.0
76	1036.0
77	815.5
78	595.0
79	399.0
80	203.0
81	187.5
82	172.0
83	127.0
84	82.0
85	85.5
86	89.0
87	51.5
88	14.0
89	10.5
90	7.0
91	7.5
92	8.0
93	8.0
94	8.0
95	5.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2186712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.529746810948772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.12383790566031	17.250459436303665
2	7.260711862993634	3.1264257615501023
3	2.7476748066874848	1.774702287204125
4	1.4860613866864014	1.2797810160354264
5	0.9626455087538198	1.0362757036083354
6	0.6811072925062653	0.8798440535250431
7	0.499964489996271	0.7534876218859392
8	0.3805776362402149	0.6554992120128951
9	0.2935033165645648	0.5687146884427945
>10	3.21743592919657	16.0637473404358
>50	1.2425625671826863	19.67710764595087
>100	1.101558499984875	34.88042224652744
>500	0.0017154891250888563	0.24413404749785506
>1k	4.288722812722141E-4	0.2421026749392496
>5k	0.0	0.0
>10k+	2.1443614063610704E-4	1.5672962640804053
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33948	1.5524678146916466	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3050	0.1394788156830895	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2194	0.10033328577334372	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5730759240357214E-5	0.0	0.0	0.033200531208499334	0.0
2	4.5730759240357214E-5	0.0	0.0	0.11290924456444196	0.0
3	4.5730759240357214E-5	0.0	0.0	0.1633045412473156	0.0
4	4.5730759240357214E-5	0.0	0.0	0.2755278244231522	0.0
5	4.5730759240357214E-5	0.0	0.0	0.4734505504154182	0.0
6	4.5730759240357214E-5	0.0	0.0	0.8031693245383937	0.0
7	4.5730759240357214E-5	0.0	0.0	0.969492095895573	0.0
8	4.5730759240357214E-5	0.0	0.0	1.3001254852033555	0.0
9	4.5730759240357214E-5	0.0	0.0	1.4386896857016378	0.0
10	4.5730759240357214E-5	0.0	0.0	1.6563223689264979	0.0
11	4.5730759240357214E-5	0.0	0.0	1.9403103838091162	0.0
12	4.5730759240357214E-5	0.0	0.0	2.1463731849461656	0.0
13	4.5730759240357214E-5	0.0	0.0	2.2565843147154268	0.0
14	4.5730759240357214E-5	0.0	0.0	2.310546610619048	0.0
15	4.5730759240357214E-5	0.0	0.0	2.369447828520628	0.0
16	9.146151848071443E-5	0.0	0.0	2.483728995862281	0.0
17	9.146151848071443E-5	0.0	0.0	2.6216986964904385	0.0
18	9.146151848071443E-5	0.0	0.0	2.783722776479024	0.0
19	9.146151848071443E-5	0.0	0.0	2.8873486769176737	0.0
20	9.146151848071443E-5	0.0	0.0	2.990105692930756	0.0
21	9.146151848071443E-5	0.0	0.0	3.1315966620204216	0.0
22	9.146151848071443E-5	0.0	0.0	3.2955414338971023	0.0
23	9.146151848071443E-5	0.0	0.0	3.4796992013580206	0.0
24	9.146151848071443E-5	0.0	0.0	3.6204127475405996	0.0
25	9.146151848071443E-5	0.0	0.0	3.7433370283786798	0.0
26	9.146151848071443E-5	0.0	0.0	3.856246272943122	0.0
27	9.146151848071443E-5	0.0	0.0	3.9685610176374393	0.0
28	9.146151848071443E-5	0.0	0.0	4.086317722681359	0.0
29	9.146151848071443E-5	0.0	0.0	4.220400308774086	0.0
30	9.146151848071443E-5	0.0	0.0	4.3922565019993485	0.0
31	9.146151848071443E-5	0.0	0.0	4.547009391268718	0.0
32	9.146151848071443E-5	0.0	0.0	4.687997322006739	0.0
33	1.3719227772107163E-4	0.0	0.0	4.834838789927526	0.0
34	1.3719227772107163E-4	0.0	0.0	4.974409067129096	0.0
35	1.3719227772107163E-4	0.0	0.0	5.1569662580166025	0.0
36	1.3719227772107163E-4	0.0	0.0	5.320590914578601	0.0
37	1.3719227772107163E-4	0.0	0.0	5.4781791109208715	0.0
38	1.3719227772107163E-4	0.0	0.0	5.638511152817563	0.0
39	1.3719227772107163E-4	0.0	0.0	5.8039193089899355	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTCGT	25	3.8919643E-5	45.000004	31
CACCGTA	25	3.8919643E-5	45.000004	34
CGGTAAC	25	3.8919643E-5	45.000004	32
ACCGGGT	20	7.034686E-4	45.0	4
CGTATCG	20	7.034686E-4	45.0	28
CGCATCG	40	6.8193913E-9	45.0	21
CACGACT	20	7.034686E-4	45.0	14
GTTCGGA	20	7.034686E-4	45.0	35
CACGAAT	30	2.1664055E-6	44.999996	38
CGTTTTT	14305	0.0	43.159733	1
CGTATGG	215	0.0	41.860466	2
TGTTACG	60	3.6379788E-12	41.249996	1
ACGGGAT	1360	0.0	40.69853	5
TCGTAAG	100	0.0	40.500004	1
GGCGATA	780	0.0	40.384617	8
TGGGATC	1495	0.0	40.33445	6
GTGTACG	95	0.0	40.263157	1
CTCACGA	140	0.0	40.17857	24
TTTGACG	140	0.0	40.17857	1
AGGGCGA	1905	0.0	40.03937	6
>>END_MODULE