##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549256_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2311439
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276703819568677	31.0	31.0	33.0	30.0	34.0
2	31.647926681171338	31.0	31.0	34.0	30.0	34.0
3	31.487791804153172	31.0	31.0	34.0	30.0	34.0
4	35.3912956387774	37.0	35.0	37.0	33.0	37.0
5	35.358712905683426	37.0	35.0	37.0	33.0	37.0
6	35.42281972399012	37.0	35.0	37.0	33.0	37.0
7	35.90001596408125	37.0	35.0	37.0	35.0	37.0
8	35.91763788704785	37.0	35.0	37.0	35.0	37.0
9	37.64629479731025	39.0	37.0	39.0	35.0	39.0
10	36.9604021564056	39.0	37.0	39.0	32.0	39.0
11	36.66402314748518	39.0	35.0	39.0	32.0	39.0
12	36.31263814446326	38.0	35.0	39.0	32.0	39.0
13	36.14713648078102	38.0	35.0	39.0	31.0	39.0
14	37.32261461366707	39.0	35.0	41.0	32.0	41.0
15	37.474485374695156	40.0	35.0	41.0	32.0	41.0
16	37.574570646251104	40.0	35.0	41.0	32.0	41.0
17	37.48722159658983	39.0	35.0	41.0	32.0	41.0
18	37.21484624945759	39.0	36.0	40.0	32.0	41.0
19	36.94342831456941	38.0	35.0	40.0	32.0	41.0
20	36.5901782396161	38.0	35.0	40.0	32.0	41.0
21	36.46499301949997	38.0	35.0	40.0	31.0	41.0
22	36.340314842831674	38.0	35.0	40.0	31.0	41.0
23	36.22940125177433	37.0	35.0	40.0	31.0	41.0
24	36.04853859435616	37.0	35.0	40.0	31.0	41.0
25	35.89259331524648	37.0	35.0	40.0	30.0	41.0
26	35.633443928219606	36.0	35.0	40.0	30.0	41.0
27	35.51190621945896	36.0	34.0	40.0	30.0	41.0
28	35.36657943385052	36.0	34.0	40.0	29.0	41.0
29	35.25222339849765	36.0	34.0	40.0	29.0	41.0
30	35.12440475392169	36.0	34.0	40.0	28.0	41.0
31	34.90170884890322	35.0	34.0	40.0	26.0	41.0
32	34.4928202734314	35.0	33.0	40.0	24.0	41.0
33	33.99921347697257	35.0	33.0	40.0	22.0	41.0
34	33.50241516215656	35.0	33.0	40.0	20.0	41.0
35	33.10475249400914	35.0	33.0	40.0	18.0	41.0
36	32.71838798255113	35.0	32.0	40.0	15.0	41.0
37	32.59507778487773	35.0	32.0	40.0	15.0	41.0
38	32.493206612850265	35.0	31.0	40.0	15.0	41.0
39	32.35431434703663	35.0	31.0	40.0	14.0	41.0
40	32.232142401335274	35.0	31.0	40.0	12.0	41.0
41	32.17166535651601	35.0	31.0	40.0	12.0	41.0
42	32.05426489732154	35.0	31.0	39.0	12.0	41.0
43	31.921895840642993	35.0	31.0	39.0	10.0	41.0
44	31.787011467748012	35.0	31.0	39.0	10.0	41.0
45	31.6607087619444	35.0	30.0	39.0	10.0	41.0
46	31.542295513747064	35.0	30.0	39.0	10.0	41.0
47	31.476886476346554	35.0	30.0	39.0	10.0	41.0
48	31.30261581638105	35.0	29.0	39.0	10.0	41.0
49	31.147296554224447	35.0	29.0	38.0	10.0	40.0
50	30.99608166168348	35.0	29.0	38.0	10.0	40.0
51	30.623171539460916	35.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	2.0
11	2.0
12	3.0
13	11.0
14	32.0
15	129.0
16	346.0
17	865.0
18	1918.0
19	3802.0
20	6643.0
21	10482.0
22	16203.0
23	24269.0
24	35810.0
25	52699.0
26	72121.0
27	77559.0
28	73114.0
29	70621.0
30	73614.0
31	82490.0
32	96100.0
33	119970.0
34	178220.0
35	257379.0
36	239260.0
37	208818.0
38	254078.0
39	354771.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.808131644399875	21.475582959359947	25.71350574252663	25.002779653713553
2	40.269373321121606	23.757321737670775	24.66182321921539	11.31148172199223
3	24.401941820658042	22.868914126654435	40.96179912167269	11.767344931014835
4	21.48332705297436	24.959689613266885	40.96387575012795	12.593107583630802
5	19.32493135228747	28.8045671981826	39.52594033413817	12.344561115391754
6	18.4933714452339	36.42890857167332	36.20242628077142	8.875293702321366
7	76.80752120216022	3.274021075183035	18.398019588663168	1.5204381339935857
8	77.08505394258728	3.075270426777432	18.043305490648898	1.7963701399863894
9	72.68766339929368	5.351817633950107	19.52982535987322	2.430693606882985
10	37.47211152879223	25.103539396886525	26.03425831267881	11.390090761642423
11	28.26771547940482	25.082816375426738	32.2938654232277	14.355602721940746
12	25.560700498693674	23.578645164332695	35.34629293699725	15.514361399976378
13	18.28679017702825	30.882839650970674	35.75902284248038	15.071347329520702
14	15.490436909648059	32.91490712062918	36.220164148826775	15.374491820895988
15	14.535231083320824	28.717867960175457	42.36187933144677	14.385021625056945
16	15.695633758883535	26.19952332724333	42.33103274626758	15.773810167605548
17	15.80249359814384	28.062734945633434	35.17362993356087	20.96114152266186
18	17.106486478769288	29.02802107258725	37.116835010571336	16.74865743807213
19	20.035830493471813	31.41056285716387	32.136950185577035	16.416656463787277
20	21.33718432543537	29.49301279419444	33.57722180857898	15.592581071791209
21	18.35432386491705	31.947154997384747	34.283188957182084	15.41533218051612
22	18.747325800075192	29.818394515278147	32.9728364019124	18.461443282734262
23	16.830165104941123	32.60484053440303	32.738696543581725	17.826297817074124
24	16.4446909479333	30.324962069083373	38.31249710678067	14.91784987620266
25	16.587199575675584	31.113691514247186	35.94886129376548	16.350247616311744
26	15.879415377174134	35.129761157443475	32.74998821080721	16.24083525457518
27	16.41998772193426	34.420203172136496	33.21433098602213	15.945478119907122
28	15.613044514694094	32.73320213079385	36.993967826968394	14.659785527543665
29	15.762734815844157	30.655794939862137	35.734968562873604	17.8465016814201
30	16.322602500001082	34.04377100152762	33.852202026529795	15.781424471941504
31	19.886096929228934	33.10755767294746	31.453609634517722	15.552735763305888
32	21.484884524315806	32.18034306767343	31.11948876868479	15.215283639325976
33	20.225193050735925	33.157266966595266	29.674977362586684	16.94256262008212
34	17.443073340892838	33.709606872601874	30.477940365287598	18.36937942121769
35	18.698178926634014	32.35880332554742	31.235693435993767	17.7073243118248
36	21.156041755806665	33.64800022842913	28.233970267006832	16.961987748757377
37	17.77169979393789	36.32633177860199	30.19828773331245	15.703680694147671
38	17.59103311833018	35.785543118377774	29.30737086291267	17.316052900379376
39	17.889202354031404	36.29215393527582	28.56056335468944	17.25808035600334
40	19.995379501687044	33.863536956848094	28.577003329960256	17.564080211504606
41	16.594467775269	33.16245853773342	30.29593253380254	19.947141153195044
42	17.618548445362393	34.36854703931188	28.388592560738136	19.624311954587597
43	18.205715141087435	34.22573556991986	29.17035664795826	18.398192641034438
44	17.425292209744665	35.47781273916378	28.83169315737945	18.2652018937121
45	16.55306499544223	36.71059456901091	27.20391929010456	19.532421145442296
46	18.37984908967963	36.0882982419177	27.448485553804364	18.08336711459831
47	18.205715141087435	34.218986527440265	28.929813851890536	18.645484479581768
48	18.491338079871454	33.067496048998045	30.249597761394526	18.19156810973597
49	17.533796046532053	33.262915439256666	30.076112759194594	19.12717575501668
50	16.99348328032883	34.97600412556853	29.755706293784957	18.27480630031768
51	16.73221746280131	35.339976525445834	28.235441212162637	19.692364799590212
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3365.0
1	3909.0
2	4453.0
3	62901.5
4	121350.0
5	76713.0
6	32076.0
7	34851.5
8	37627.0
9	45469.5
10	53312.0
11	60005.5
12	66699.0
13	68433.0
14	70167.0
15	65526.5
16	60886.0
17	53501.0
18	46116.0
19	41100.0
20	36084.0
21	33338.0
22	30592.0
23	28442.5
24	26293.0
25	27633.0
26	29320.5
27	29668.0
28	33315.0
29	36962.0
30	39078.5
31	41195.0
32	45799.5
33	50404.0
34	54232.5
35	58061.0
36	61831.5
37	65602.0
38	69820.0
39	74038.0
40	90553.5
41	107069.0
42	118688.0
43	130307.0
44	130677.5
45	131048.0
46	132607.5
47	134167.0
48	141597.0
49	149027.0
50	145911.5
51	142796.0
52	132903.5
53	123011.0
54	108108.5
55	93206.0
56	84137.5
57	75069.0
58	68873.0
59	62677.0
60	56020.5
61	49364.0
62	45283.5
63	41203.0
64	35149.5
65	29096.0
66	25665.5
67	22235.0
68	18784.0
69	15333.0
70	13148.5
71	10964.0
72	9452.5
73	7941.0
74	6710.5
75	4522.5
76	3565.0
77	2645.5
78	1726.0
79	1457.0
80	1188.0
81	965.5
82	743.0
83	438.0
84	133.0
85	111.5
86	90.0
87	61.0
88	32.0
89	27.5
90	23.0
91	16.5
92	10.0
93	10.5
94	11.0
95	6.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2311439.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.595384698123162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.74105007277335	22.51630618365132
2	7.125081294531993	4.074888812450874
3	3.1452767610183257	2.6982119689015738
4	1.9559482347613362	2.237243688904613
5	1.4209302341801315	2.0316023337787548
6	1.0657714550433788	1.8285686854346332
7	0.7902532938598436	1.5818317862816833
8	0.6213581187517491	1.4214379552806684
9	0.47387134675758136	1.2195480112155677
>10	3.4064712350301742	20.351993751339723
>50	0.8216882366281295	17.032888822999354
>100	0.4278462811763547	17.012802581843463
>500	0.0023035011142909403	0.4425113044645049
>1k	0.0019963676323854814	1.1902618743187856
>5k	0.0	0.0
>10k+	1.5356674095272935E-4	4.359902239134501
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99285	4.295376170428898	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	4842	0.20947989542445203	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	4013	0.1736147914783821	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	3157	0.13658158402622783	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2539	0.10984499266474261	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03698994435933633	0.0
2	0.0	0.0	0.0	0.13126022360962153	0.0
3	0.0	0.0	0.0	0.2677985445430314	0.0
4	0.0	0.0	0.0	0.4205172621903498	0.0
5	0.0	0.0	0.0	0.7767888315460628	0.0
6	0.0	0.0	0.0	1.5469151467981634	0.0
7	0.0	0.0	0.0	2.069706360410117	0.0
8	0.0	0.0	0.0	2.707620664010601	0.0
9	0.0	0.0	0.0	3.10551132865717	0.0
10	0.0	0.0	0.0	3.5578269640687035	0.0
11	4.3263092817937224E-5	0.0	0.0	3.973239181306537	0.0
12	4.3263092817937224E-5	0.0	0.0	4.289362600527204	0.0
13	4.3263092817937224E-5	0.0	0.0	4.485690515735003	0.0
14	4.3263092817937224E-5	0.0	0.0	4.586190680351072	0.0
15	4.3263092817937224E-5	0.0	0.0	4.675745282484201	0.0
16	4.3263092817937224E-5	0.0	0.0	4.817691490019853	0.0
17	4.3263092817937224E-5	0.0	0.0	4.97002083983181	0.0
18	4.3263092817937224E-5	0.0	0.0	5.154364878329041	0.0
19	4.3263092817937224E-5	0.0	0.0	5.266978708934132	0.0
20	4.3263092817937224E-5	0.0	0.0	5.380025170467402	0.0
21	4.3263092817937224E-5	0.0	0.0	5.525086320685945	0.0
22	4.3263092817937224E-5	0.0	0.0	5.671229048224937	0.0
23	4.3263092817937224E-5	0.0	0.0	5.848045308571846	0.0
24	4.3263092817937224E-5	0.0	0.0	5.991332671984854	0.0
25	4.3263092817937224E-5	0.0	0.0	6.118396375591136	0.0
26	4.3263092817937224E-5	0.0	0.0	6.2351634631067485	0.0
27	4.3263092817937224E-5	0.0	0.0	6.3580306467096905	0.0
28	4.3263092817937224E-5	0.0	0.0	6.4815900397977195	0.0
29	4.3263092817937224E-5	0.0	0.0	6.614234682377514	0.0
30	4.3263092817937224E-5	0.0	0.0	6.794382200871405	0.0
31	4.3263092817937224E-5	0.0	0.0	6.9388809308833155	0.0
32	4.3263092817937224E-5	0.0	0.0	7.082298083574777	0.0
33	8.652618563587445E-5	0.0	0.0	7.227315970700503	0.0
34	8.652618563587445E-5	0.0	0.0	7.366666392667079	0.0
35	8.652618563587445E-5	0.0	0.0	7.526653309907811	0.0
36	8.652618563587445E-5	0.0	0.0	7.673661299303161	0.0
37	8.652618563587445E-5	0.0	0.0	7.8260339122079365	0.0
38	8.652618563587445E-5	0.0	0.0	7.985631461613306	0.0
39	8.652618563587445E-5	0.0	0.0	8.143628276584414	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATC	45	3.8562575E-10	45.000004	33
CTACGTA	40	6.8193913E-9	45.0	28
CGTACTC	25	3.892035E-5	45.0	37
GCGTAAT	25	3.892035E-5	45.0	9
CCGAATA	65	0.0	44.999996	16
CGTTTTT	36215	0.0	43.831978	1
TATTGCG	245	0.0	42.2449	1
CGTTAGG	290	0.0	41.120693	2
GTTGATC	750	0.0	41.100002	16
TGCGTAG	115	0.0	41.086956	1
CGTCGTA	50	1.0822987E-9	40.5	30
TCGACGG	100	0.0	40.5	2
TTAATCG	460	0.0	40.108692	20
CGACGGT	180	0.0	40.000004	28
TAGCACG	45	1.9297659E-8	40.000004	1
CACTACG	45	1.9297659E-8	40.000004	1
ACCGTAG	45	1.9297659E-8	40.000004	1
GTTTTTT	40240	0.0	39.732853	2
CGTAAGG	280	0.0	39.375	2
CTCATGC	770	0.0	39.15584	23
>>END_MODULE