FastQCFastQC Report
Sat 18 Jun 2016
SRR3549253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250507
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133151.064768130046453No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCT21710.17360958395274878No Hit
TCTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGC17500.13994323902225256No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTT16430.13138670955060627No Hit
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC15590.12466943407753814Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTC15140.12107089364553737No Hit
CGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCT13730.1097954669586016Illumina PCR Primer Index 5 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG13350.10675669948268982Illumina PCR Primer Index 5 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG13100.1047575103538005Illumina PCR Primer Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG207.8567484E-444.01
GCGTATC207.8567484E-444.044
CGTTTTT112350.042.9034231
CGAGGGA8700.038.4367834
AGGGCGA13950.038.322586
CGACGGT750.038.1333328
GTTTTTT126950.037.9519462
CGCATCG700.037.71428721
CTAACGG700.037.7142872
CCGCATC700.037.71428720
CGCCAGT700.037.71428726
AAGGGCG5700.037.43865
TTAACGG651.0913936E-1137.2307662
TAACGGG2500.036.963
GGGCGAT28050.036.7058837
CTATGCG301.3011607E-436.6666681
GAGTACG601.9826984E-1036.6666681
CGATTCG601.9826984E-1036.66666810
GCGATTC1200.036.6666689
TACAACG301.3011607E-436.6666681
Produced by FastQC (version 0.11.5)