##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549253_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1250507
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.403322812267344	31.0	31.0	33.0	30.0	34.0
2	31.82061755751867	31.0	31.0	34.0	30.0	34.0
3	31.916223579716068	31.0	31.0	34.0	30.0	34.0
4	35.606182132527046	37.0	35.0	37.0	33.0	37.0
5	35.491869297812805	37.0	35.0	37.0	33.0	37.0
6	35.53714293482563	37.0	35.0	37.0	33.0	37.0
7	35.984426316685955	37.0	35.0	37.0	35.0	37.0
8	35.98819518803173	37.0	35.0	37.0	35.0	37.0
9	37.65769963702722	39.0	37.0	39.0	35.0	39.0
10	37.108733497693336	39.0	37.0	39.0	33.0	39.0
11	36.92382449678411	39.0	37.0	39.0	33.0	39.0
12	36.66374758397994	39.0	35.0	39.0	32.0	39.0
13	36.640642555379536	39.0	35.0	39.0	32.0	39.0
14	37.73730494911264	40.0	36.0	41.0	33.0	41.0
15	37.82006498164345	40.0	36.0	41.0	33.0	41.0
16	37.828740662787176	40.0	36.0	41.0	33.0	41.0
17	37.7123550687841	40.0	36.0	41.0	32.0	41.0
18	37.5522640017209	39.0	36.0	41.0	32.0	41.0
19	37.4505884413282	39.0	36.0	41.0	32.0	41.0
20	37.238616817019015	39.0	35.0	41.0	32.0	41.0
21	37.11287821659535	39.0	35.0	41.0	32.0	41.0
22	37.054729001916826	39.0	35.0	41.0	32.0	41.0
23	36.9302738809139	39.0	35.0	41.0	32.0	41.0
24	36.8476441955143	38.0	35.0	41.0	31.0	41.0
25	36.68519408527901	38.0	35.0	41.0	31.0	41.0
26	36.60319534396849	38.0	35.0	40.0	31.0	41.0
27	36.42909715819264	38.0	35.0	40.0	31.0	41.0
28	36.37160127852143	38.0	35.0	40.0	30.0	41.0
29	36.2802151447373	38.0	35.0	40.0	30.0	41.0
30	36.201792552940525	38.0	35.0	40.0	30.0	41.0
31	36.057864530146574	38.0	35.0	40.0	30.0	41.0
32	35.74650761651074	38.0	35.0	40.0	27.0	41.0
33	35.46912492293126	38.0	35.0	41.0	25.0	41.0
34	35.223928374651244	38.0	35.0	41.0	24.0	41.0
35	34.917091227798004	38.0	34.0	41.0	23.0	41.0
36	34.7668137803307	38.0	34.0	40.0	22.0	41.0
37	34.68392420034434	38.0	34.0	40.0	22.0	41.0
38	34.56839026091018	38.0	34.0	40.0	21.0	41.0
39	34.485491084816	38.0	34.0	40.0	21.0	41.0
40	34.462616362803246	38.0	34.0	40.0	21.0	41.0
41	34.46355438234252	38.0	34.0	40.0	21.0	41.0
42	34.299828789443005	38.0	33.0	40.0	20.0	41.0
43	34.29398316043013	38.0	33.0	40.0	20.0	41.0
44	34.0558237578838	38.0	33.0	40.0	18.0	41.0
45	33.870568497417445	37.0	33.0	40.0	18.0	41.0
46	33.905563103605175	37.0	33.0	40.0	19.0	41.0
47	33.76067706938066	37.0	33.0	40.0	18.0	41.0
48	33.720627713399445	37.0	33.0	40.0	18.0	41.0
49	33.66210984824555	37.0	33.0	40.0	18.0	41.0
50	33.06197166429296	36.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	4.0
13	2.0
14	10.0
15	25.0
16	83.0
17	218.0
18	586.0
19	1094.0
20	2032.0
21	3250.0
22	5098.0
23	7546.0
24	11461.0
25	17910.0
26	26671.0
27	31326.0
28	30898.0
29	30243.0
30	32096.0
31	36569.0
32	43975.0
33	54550.0
34	80175.0
35	114179.0
36	97135.0
37	120830.0
38	182944.0
39	319593.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.13656540907009	23.290953189386386	26.178501999588967	18.393979401954567
2	36.40003614533945	24.403062118004936	25.851034820276897	13.345866916378718
3	27.58713065980438	24.968352836089682	34.430355047992535	13.014161456113401
4	23.44856926030802	27.18081546124892	34.696007299439344	14.674607979003715
5	21.684564740541237	30.537693911349557	33.52944045894985	14.248300889159356
6	19.333998130358328	38.821613953380506	30.810943081486148	11.033444834775015
7	81.56339788581751	3.1242528030630776	12.942830387994631	2.369518923124781
8	80.78795240650393	2.697066070002007	12.558506269856945	3.956475253637125
9	74.05828196083668	5.347351114388004	16.047970942985526	4.546395981789786
10	32.268911729402554	32.09058405910562	21.469771860533367	14.17073235095845
11	27.059504664907912	25.66055208007632	32.30785593363332	14.972087321382446
12	24.842403920969655	22.251294874798784	33.56374654440159	19.342554659829972
13	21.022113430792473	26.26750589960712	35.90111850633383	16.809262163266578
14	18.22956608799471	29.072688117699464	34.02467958995831	18.673066204347517
15	17.55256068138763	24.566915659008707	40.75163113841026	17.128892521193404
16	18.98206087610865	23.94092955897088	39.428167935085526	17.648841629834937
17	18.585261817806696	24.61273707384285	34.59308904308413	22.20891206526633
18	21.189485544663082	23.33221645300666	35.12183458389278	20.35646341843748
19	22.46368872785198	27.830791830833412	31.105463623954122	18.600055817360477
20	24.979628262776618	25.172350094801548	30.103150162294174	19.74487148012766
21	22.435860015177845	28.436706072017188	30.88819174942643	18.239242163378535
22	21.64330147692096	24.321095363720474	30.74441006727671	23.29119309208185
23	19.713364259456366	29.440299014719628	30.175200938499348	20.67113578732466
24	19.60196944119465	25.427846465473603	35.147184302047094	19.822999791284655
25	20.581812017045888	26.096935083130283	32.866829214070776	20.45442368575306
26	19.436196678627148	28.173452847525045	32.14528187367204	20.24506860017577
27	20.803242204961666	26.121884963458818	30.849567415456292	22.22530541612322
28	19.377020680412024	27.83231121457137	33.658348173980634	19.132319931035973
29	19.79309192191647	24.813855500209115	32.34272179204115	23.050330785833268
30	21.13830630296352	25.494139576987575	31.556560658996712	21.8109934610522
31	22.813866695668235	26.549071696519892	28.218714489403098	22.418347118408775
32	22.44193755012967	25.704454273346734	28.735784765699034	23.11782341082457
33	20.986287961602773	27.725074709697743	28.32035326471583	22.96828406398365
34	20.034114163295367	28.13306922712148	28.625269590654028	23.207547018929123
35	19.466984191212045	28.91579175486423	29.714747698333554	21.902476355590174
36	22.957648377817957	28.916351527820318	26.51204671385286	21.613953380508867
37	21.130389514013117	29.76960544803028	27.74906497924442	21.350940058712187
38	21.08568764509115	30.699548263224436	27.21832024930688	20.99644384237753
39	21.08040978579088	28.88796304219009	26.517484508283438	23.514142663735587
40	22.95093110234489	27.23527337311986	28.280129579442576	21.533665945092668
41	19.270743786320267	27.107005398610323	28.23998586173448	25.382264953334925
42	22.018429325065753	28.27517159040293	26.54331403182869	23.163085052702623
43	21.318473227259023	27.14339064075611	28.635985244384877	22.90215088759999
44	22.42090608049375	28.4332674667155	27.641908441935954	21.503918010854797
45	20.596446081469356	27.380654406572695	28.449021077051146	23.573878434906803
46	23.185236068250717	27.473016944327384	26.810805537274078	22.53094145014782
47	21.99715795273437	26.49981167638406	28.64006359020781	22.86296678067376
48	22.297516127458703	25.14748018203817	28.58952408902949	23.965479601473643
49	21.855135557018073	24.754759469559147	28.395282873266602	24.994822100156178
50	20.38581151484958	25.87686434382215	30.567281910457115	23.170042230871157
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	805.0
1	902.0
2	999.0
3	11511.5
4	22024.0
5	16128.0
6	10232.0
7	10443.5
8	10655.0
9	11091.0
10	11527.0
11	11757.0
12	11987.0
13	11858.0
14	11729.0
15	11267.0
16	10805.0
17	10296.0
18	9787.0
19	9517.5
20	9248.0
21	9169.5
22	9091.0
23	9101.0
24	9111.0
25	9614.0
26	10117.0
27	11068.5
28	12020.0
29	14273.5
30	16527.0
31	18540.0
32	20553.0
33	23749.5
34	26946.0
35	29933.5
36	32921.0
37	36231.5
38	39542.0
39	45461.5
40	51381.0
41	58454.0
42	65527.0
43	75309.0
44	85091.0
45	95231.5
46	105372.0
47	117166.0
48	128960.0
49	128591.5
50	128223.0
51	111783.0
52	95343.0
53	84134.0
54	72925.0
55	63711.5
56	54498.0
57	48387.0
58	42276.0
59	39990.5
60	37705.0
61	34044.0
62	30383.0
63	25878.0
64	21373.0
65	17540.5
66	13708.0
67	11343.0
68	8978.0
69	7951.5
70	6925.0
71	6201.5
72	5478.0
73	4829.0
74	4180.0
75	3468.0
76	2756.0
77	2125.5
78	1495.0
79	1097.5
80	700.0
81	548.5
82	397.0
83	275.0
84	153.0
85	99.0
86	45.0
87	25.0
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1250507.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.405974804762565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.66812109157163	29.061079521666233
2	7.978069368079091	6.128110622821872
3	3.5622231257281234	4.1043195484697055
4	2.2061542662599716	3.3891802066159964
5	1.5289863805044182	2.93611062032389
6	1.1939413974088795	2.751268993634906
7	0.9237800548849577	2.483507145913771
8	0.7620700331671316	2.3414433994625146
9	0.6408411039900729	2.215091456432907
>10	5.412168535157423	38.07589923703073
>50	0.09636334388122005	2.4214202649698087
>100	0.023294033474109365	1.4879872579050328
>500	0.0018887048966921755	0.41676853876394604
>1k	0.0018887048966921755	1.1146603927877343
>5k	0.0	0.0
>10k+	2.0985609963246395E-4	1.073152793200917
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13315	1.064768130046453	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCT	2171	0.17360958395274878	No Hit
TCTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGC	1750	0.13994323902225256	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTT	1643	0.13138670955060627	No Hit
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	1559	0.12466943407753814	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTC	1514	0.12107089364553737	No Hit
CGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCT	1373	0.1097954669586016	Illumina PCR Primer Index 5 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG	1335	0.10675669948268982	Illumina PCR Primer Index 5 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG	1310	0.1047575103538005	Illumina PCR Primer Index 5 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1348253148522959	0.0
2	0.0	0.0	0.0	0.44166086235422913	0.0
3	0.0	0.0	0.0	0.8003953596421292	0.0
4	0.0	0.0	0.0	1.1940756829030146	0.0
5	0.0	0.0	0.0	1.9596851517024694	0.0
6	0.0	0.0	0.0	3.5650340222005954	0.0
7	0.0	0.0	0.0	4.4973758643494195	0.0
8	0.0	0.0	0.0	6.740785937223862	0.0
9	0.0	0.0	0.0	8.021706395885829	0.0
10	0.0	0.0	0.0	9.680473599907877	0.0
11	0.0	0.0	0.0	10.459677554783779	0.0
12	0.0	0.0	0.0	11.220009164282967	0.0
13	0.0	0.0	0.0	11.753312856305483	0.0
14	0.0	0.0	0.0	12.027281734528476	0.0
15	0.0	0.0	0.0	12.245433252272878	0.0
16	0.0	0.0	0.0	12.600729144259088	0.0
17	0.0	0.0	0.0	12.957784322678721	0.0
18	0.0	0.0	0.0	13.553462715522585	0.0
19	0.0	0.0	0.0	13.801122264809393	0.0
20	0.0	0.0	0.0	14.132267952118621	0.0
21	0.0	0.0	0.0	14.415593035464816	0.0
22	0.0	0.0	0.0	14.735383328521952	0.0
23	0.0	0.0	0.0	15.096276950069052	0.0
24	0.0	0.0	0.0	15.375283784896846	0.0
25	0.0	0.0	0.0	15.6271816151369	0.0
26	0.0	0.0	0.0	15.86636460251722	0.0
27	0.0	0.0	0.0	16.145371437345013	0.0
28	0.0	0.0	0.0	16.41094372122667	0.0
29	0.0	0.0	0.0	16.682273669799528	0.0
30	0.0	0.0	0.0	17.025414491882092	0.0
31	0.0	0.0	0.0	17.322893834260825	0.0
32	0.0	0.0	0.0	17.639005619320802	0.0
33	0.0	0.0	0.0	17.9381642805678	0.0
34	0.0	0.0	0.0	18.22900631503862	0.0
35	0.0	0.0	0.0	18.518248998206328	0.0
36	0.0	0.0	0.0	18.802613659899546	0.0
37	0.0	0.0	0.0	19.08897751072165	0.0
38	0.0	0.0	0.0	19.37782035606358	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	20	7.8567484E-4	44.0	1
GCGTATC	20	7.8567484E-4	44.0	44
CGTTTTT	11235	0.0	42.903423	1
CGAGGGA	870	0.0	38.436783	4
AGGGCGA	1395	0.0	38.32258	6
CGACGGT	75	0.0	38.13333	28
GTTTTTT	12695	0.0	37.951946	2
CGCATCG	70	0.0	37.714287	21
CTAACGG	70	0.0	37.714287	2
CCGCATC	70	0.0	37.714287	20
CGCCAGT	70	0.0	37.714287	26
AAGGGCG	570	0.0	37.4386	5
TTAACGG	65	1.0913936E-11	37.230766	2
TAACGGG	250	0.0	36.96	3
GGGCGAT	2805	0.0	36.705883	7
CTATGCG	30	1.3011607E-4	36.666668	1
GAGTACG	60	1.9826984E-10	36.666668	1
CGATTCG	60	1.9826984E-10	36.666668	10
GCGATTC	120	0.0	36.666668	9
TACAACG	30	1.3011607E-4	36.666668	1
>>END_MODULE