FastQCFastQC Report
Sat 18 Jun 2016
SRR3549252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807902
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87040.481442025065518No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31540.17445635880705923No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTG21560.11925425161319586No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC21170.11709705503948775No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT20400.11283797462473077No Hit
AAGAGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT18750.10371137373596578No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAC207.857679E-444.00000443
CACTGCG254.4438555E-544.01
TTACGAG551.8189894E-1244.01
TACTACG302.5285244E-644.01
TCGTTAG1000.041.8000031
CGTTTTT75700.041.645971
CGGTCTA2150.040.93023331
TATTACG557.8216544E-1140.01
TCACGAC2250.039.1111125
GACCGAC452.3521352E-839.111119
CGCAATA452.3521352E-839.1111120
ACGGGAT7500.039.0133365
CGTAACT404.1262865E-738.50000433
CGACGGT2300.038.2608728
ATTACGG1150.038.260872
CACGACG2250.038.13333526
CGAGGGA10600.037.9811364
GCGATAA2150.037.8604669
ACGTTAG700.037.7142871
CTACGCG357.2925213E-637.7142871
Produced by FastQC (version 0.11.5)