FastQCFastQC Report
Sat 18 Jun 2016
SRR3549250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987228
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71000.7191854363936193No Hit
TCTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGC14230.14414096844903102No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA11010.11152438950272886No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGT207.85594E-444.00000427
TTACGTC207.85594E-444.00000426
GATTCGC207.85594E-444.0000049
CGTTTTT45050.041.6071051
AACGGAC603.6379788E-1240.33333638
CGTTAGG1700.040.117652
GCGATCA1100.040.09
GCTACGA557.8216544E-1140.010
ATGGGCG4300.039.9069755
TATGCCA1600.039.87500410
TTTACGG900.039.1111072
TGATGCG900.039.1111071
TAACGGG2650.039.0188673
ACGGGTA850.038.8235325
GGCGATA4550.038.681328
ATTGCGG1650.038.6666682
CTCACGA2050.038.63414824
AGGGCGA10450.038.5263186
GGGCGAT19450.038.4575847
TATGGGC3950.038.430384
Produced by FastQC (version 0.11.5)