##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549250_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	987228
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.293343584258146	31.0	31.0	33.0	30.0	34.0
2	31.679282799920585	31.0	31.0	34.0	30.0	34.0
3	31.80661913965163	31.0	31.0	34.0	30.0	34.0
4	35.49854440919423	37.0	35.0	37.0	33.0	37.0
5	35.38479662246208	37.0	35.0	37.0	33.0	37.0
6	35.422238834392864	37.0	35.0	37.0	33.0	37.0
7	35.938757814810764	37.0	35.0	37.0	35.0	37.0
8	35.92684263412302	37.0	35.0	37.0	35.0	37.0
9	37.61321903349581	39.0	37.0	39.0	35.0	39.0
10	36.93743491878269	39.0	37.0	39.0	32.0	39.0
11	36.74056246378749	39.0	35.0	39.0	32.0	39.0
12	36.29360492206461	38.0	35.0	39.0	32.0	39.0
13	36.13230074511663	38.0	35.0	39.0	32.0	39.0
14	37.186436162669615	39.0	35.0	41.0	32.0	41.0
15	37.36780358741851	39.0	35.0	41.0	32.0	41.0
16	37.42454427953826	39.0	35.0	41.0	32.0	41.0
17	37.32215759682667	39.0	35.0	41.0	32.0	41.0
18	37.28587924977817	39.0	35.0	41.0	32.0	41.0
19	37.26241759755599	39.0	35.0	41.0	32.0	41.0
20	37.10748783462382	39.0	35.0	41.0	32.0	41.0
21	36.979032199248806	39.0	35.0	41.0	32.0	41.0
22	36.90881235135146	38.0	35.0	41.0	31.0	41.0
23	36.81760140514653	38.0	35.0	41.0	31.0	41.0
24	36.73312446567561	38.0	35.0	41.0	31.0	41.0
25	36.559000555089604	38.0	35.0	40.0	31.0	41.0
26	36.46057952165052	38.0	35.0	40.0	31.0	41.0
27	36.37714489459375	38.0	35.0	40.0	30.0	41.0
28	36.442680920719425	38.0	35.0	40.0	31.0	41.0
29	36.347553959166476	38.0	35.0	40.0	30.0	41.0
30	36.29125288180643	38.0	35.0	40.0	30.0	41.0
31	36.18673700502822	38.0	35.0	40.0	30.0	41.0
32	35.941819924070224	38.0	35.0	40.0	30.0	41.0
33	35.84507935350294	38.0	35.0	40.0	29.0	41.0
34	35.733002913207486	38.0	35.0	40.0	29.0	41.0
35	35.478743512137015	38.0	35.0	40.0	27.0	41.0
36	35.340457320902566	38.0	34.0	40.0	27.0	41.0
37	35.22618787149473	38.0	34.0	40.0	26.0	41.0
38	35.18154468876491	38.0	34.0	40.0	27.0	41.0
39	35.05733933802526	37.0	34.0	40.0	26.0	41.0
40	34.994150287471584	37.0	34.0	40.0	26.0	41.0
41	35.014871944474834	37.0	34.0	40.0	26.0	41.0
42	34.844288249522904	37.0	34.0	40.0	25.0	41.0
43	34.86695879776506	37.0	34.0	40.0	26.0	41.0
44	34.60376731616202	37.0	34.0	40.0	24.0	41.0
45	34.46386549003878	36.0	34.0	40.0	24.0	41.0
46	34.45175076071586	36.0	34.0	40.0	24.0	41.0
47	34.30734035096249	36.0	33.0	40.0	24.0	41.0
48	34.26994372120726	36.0	33.0	40.0	24.0	41.0
49	34.24336728698943	36.0	33.0	40.0	24.0	41.0
50	33.67607381476214	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	7.0
14	8.0
15	29.0
16	59.0
17	166.0
18	377.0
19	880.0
20	1353.0
21	2309.0
22	3554.0
23	5123.0
24	7345.0
25	10560.0
26	14448.0
27	17483.0
28	19719.0
29	21684.0
30	25569.0
31	30943.0
32	37631.0
33	48132.0
34	75813.0
35	115650.0
36	79669.0
37	98462.0
38	142122.0
39	228127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.570636165100666	23.357826155660092	27.61084572155571	14.460691957683533
2	32.78492911465234	24.99128873978453	28.3696370038127	13.854145141750438
3	29.578476299294593	25.359491424473372	31.637372521849056	13.424659754382978
4	25.468584764613645	28.22063393663875	31.299456660467488	15.011324638280113
5	23.281450688189555	31.922919528214354	30.29502809887888	14.500601684717207
6	19.09133452454752	41.2042608191826	28.167556025558433	11.536848630711447
7	85.61386022276515	4.098850518826452	7.993796772376796	2.2934924860315955
8	84.99910861523377	3.60271386143829	7.556511768304788	3.8416657550231554
9	79.07190638839255	5.550693456830641	10.990065111605425	4.387335043171385
10	39.643932303378755	29.05245799349289	16.37119287540467	14.932416827723689
11	35.570607802858106	24.643648680953135	24.055537322685336	15.730206193503427
12	32.00040922664268	20.92556126852156	27.06588548947153	20.00814401536423
13	22.974935881073066	30.16344755213588	28.813607393631465	18.048009173159592
14	18.186072518202483	32.623061744602055	29.00657193677651	20.18429380041895
15	17.127147933405453	23.245694003816748	39.81785362651789	19.80930443625991
16	19.28379259907539	21.214856142653975	38.32903847945966	21.172312778810973
17	18.39412982613945	22.81013099304315	30.27132536759492	28.524413813222477
18	22.63580449501027	22.832820787092746	31.81200290105224	22.719371816844742
19	25.779657789284744	27.799454634593022	25.362023767559265	21.05886380856297
20	28.379867669879705	24.51328365889136	24.848160708569857	22.25868796265908
21	23.705466214491487	29.40141487072895	26.099239486724446	20.79387942805512
22	23.224726203065554	24.2505277402991	25.229734164752216	27.29501189188313
23	21.34805738897195	30.339192162296857	24.08997718865348	24.222773260077712
24	21.098165773256024	25.3323447065926	32.51609557265394	21.053393947497437
25	21.355553124506194	26.103088648215	29.45084620776558	23.090512019513223
26	19.57096030501566	29.098850518826453	27.44401495905708	23.886174217100812
27	22.158508470181154	27.0632518526622	26.587272646237746	24.190967030918898
28	20.324079138760247	27.98502473592726	31.028597244000373	20.66229888131212
29	20.94379413873999	25.159233733240953	29.636821483993565	24.260150644025494
30	23.210241200614245	26.307701969555158	28.27553513474091	22.206521695089684
31	27.93417528676253	24.711110300761323	23.923551601048594	23.43116281142755
32	27.776663546819986	24.803591470258137	24.36964915905951	23.05009582386237
33	26.909791861657084	25.482056829830597	23.138018775804575	24.470132532707744
34	23.792072348029027	26.321680503389288	26.30324504572399	23.583002102857698
35	23.43977277791959	26.258979688582578	27.217927368348548	23.08332016514929
36	28.785042563622586	27.139222145238996	23.73139740769103	20.344337883447388
37	23.6947290798073	29.444971171806312	26.48516857301454	20.37513117537185
38	23.643373162025387	28.93515986175433	26.375264883086786	21.046202093133502
39	22.5238749306138	28.56412095280928	26.173791667173134	22.738212449403786
40	24.223178434971455	24.829725250904552	28.517931014922592	22.4291652992014
41	19.913839558845574	24.858087493466556	27.873702933871407	27.354370013816464
42	23.08058523461652	26.985559566787003	24.88563938624107	25.0482158123554
43	23.286312786914472	25.631059897004544	26.881125737924776	24.20150157815621
44	24.09240823801594	27.461842654381762	27.109846965442635	21.335902142159664
45	21.129364240074228	28.17900221630667	26.341027604565507	24.350605939053594
46	25.17999894654528	27.16393781375731	25.766388311514664	21.88967492818275
47	22.93978695904087	25.921975470711935	27.272929860174145	23.865307710073054
48	23.853658931877945	24.388489791618554	27.38445424967687	24.37339702682663
49	24.0327462349123	23.882325055610252	27.65470590380338	24.43022280567407
50	21.90385604946375	25.6817067587224	28.343807104336587	24.07063008747726
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1139.0
1	1213.5
2	1288.0
3	6215.0
4	11142.0
5	7660.0
6	4178.0
7	4294.5
8	4411.0
9	4602.5
10	4794.0
11	4841.0
12	4888.0
13	4779.5
14	4671.0
15	4550.5
16	4430.0
17	4242.0
18	4054.0
19	3872.0
20	3690.0
21	3722.5
22	3755.0
23	3918.5
24	4082.0
25	4663.0
26	5244.0
27	6030.5
28	6817.0
29	8171.0
30	9525.0
31	11181.0
32	12837.0
33	14199.0
34	15561.0
35	17522.0
36	19483.0
37	22313.0
38	25143.0
39	29987.5
40	34832.0
41	42906.5
42	50981.0
43	61842.5
44	72704.0
45	77771.5
46	82839.0
47	89467.5
48	96096.0
49	96266.5
50	96437.0
51	85731.5
52	75026.0
53	68210.5
54	61395.0
55	55820.0
56	50245.0
57	48231.5
58	46218.0
59	44618.0
60	43018.0
61	38822.0
62	34626.0
63	31163.0
64	27700.0
65	23259.5
66	18819.0
67	15967.5
68	13116.0
69	11760.0
70	10404.0
71	9367.0
72	8330.0
73	7081.5
74	5833.0
75	4866.5
76	3900.0
77	2889.5
78	1879.0
79	1398.5
80	918.0
81	664.0
82	410.0
83	322.0
84	234.0
85	151.5
86	69.0
87	56.0
88	43.0
89	32.0
90	21.0
91	11.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	987228.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.204052190878816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25433392973397	23.794442157241207
2	7.694798601423888	4.802177943142647
3	3.0599107060920203	2.8644484011697275
4	1.8393186568793167	2.295767814596773
5	1.2751979185125544	1.989567120148289
6	0.9876516917771135	1.8491240961973687
7	0.7960782749015766	1.7388607626637496
8	0.6308735606177621	1.5748649209089762
9	0.5699515518407354	1.6006318172919616
>10	6.338194555446205	43.800657519530574
>50	0.5058407934793513	9.838766548201717
>100	0.04557119932905542	2.5448830279375483
>500	0.0013020342665428157	0.32828026127671833
>1k	6.510171332714078E-4	0.2563673822594174
>5k	3.255085666357039E-4	0.721160227433385
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7100	0.7191854363936193	No Hit
TCTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGC	1423	0.14414096844903102	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1101	0.11152438950272886	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07779357959863376	0.0
2	0.0	0.0	0.0	0.27379693444675396	0.0
3	0.0	0.0	0.0	0.45632822407792323	0.0
4	0.0	0.0	0.0	0.6944697678753033	0.0
5	0.0	0.0	0.0	1.122638336838096	0.0
6	0.0	0.0	0.0	2.1065042725692544	0.0
7	0.0	0.0	0.0	2.738779694255025	0.0
8	0.0	0.0	0.0	4.814794556070128	0.0
9	0.0	0.0	0.0	6.016948465805265	0.0
10	0.0	0.0	0.0	7.668340038977825	0.0
11	0.0	0.0	0.0	8.429663664320705	0.0
12	0.0	0.0	0.0	9.197166206793161	0.0
13	0.0	0.0	0.0	9.613584703837411	0.0
14	0.0	0.0	0.0	9.804928547407489	0.0
15	0.0	0.0	0.0	9.963858399478134	1.0129372343572103E-4
16	0.0	0.0	0.0	10.238870858606118	1.0129372343572103E-4
17	0.0	0.0	0.0	10.51459237379815	1.0129372343572103E-4
18	0.0	0.0	0.0	11.011640674697233	1.0129372343572103E-4
19	0.0	0.0	0.0	11.192449971029996	1.0129372343572103E-4
20	0.0	0.0	0.0	11.427957878018047	1.0129372343572103E-4
21	0.0	0.0	0.0	11.652728650321912	1.0129372343572103E-4
22	0.0	0.0	0.0	11.882361521350692	1.0129372343572103E-4
23	0.0	0.0	0.0	12.151195063349094	1.0129372343572103E-4
24	0.0	0.0	0.0	12.365633875862516	1.0129372343572103E-4
25	0.0	0.0	0.0	12.538744849214163	1.0129372343572103E-4
26	0.0	0.0	0.0	12.71307134724704	1.0129372343572103E-4
27	0.0	0.0	0.0	12.92659851624954	1.0129372343572103E-4
28	0.0	0.0	0.0	13.118246240989924	1.0129372343572103E-4
29	0.0	0.0	0.0	13.3067538603038	1.0129372343572103E-4
30	0.0	0.0	0.0	13.599087546139291	1.0129372343572103E-4
31	1.0129372343572103E-4	0.0	0.0	13.809373315991849	1.0129372343572103E-4
32	1.0129372343572103E-4	0.0	0.0	14.043665698298671	1.0129372343572103E-4
33	1.0129372343572103E-4	0.0	0.0	14.2569902798543	1.0129372343572103E-4
34	1.0129372343572103E-4	0.0	0.0	14.456437621299234	1.0129372343572103E-4
35	1.0129372343572103E-4	0.0	0.0	14.67432042040947	1.0129372343572103E-4
36	1.0129372343572103E-4	0.0	0.0	14.86941213174667	1.0129372343572103E-4
37	1.0129372343572103E-4	0.0	0.0	15.08314188819604	1.0129372343572103E-4
38	1.0129372343572103E-4	0.0	0.0	15.30375961783904	1.0129372343572103E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCGT	20	7.85594E-4	44.000004	27
TTACGTC	20	7.85594E-4	44.000004	26
GATTCGC	20	7.85594E-4	44.000004	9
CGTTTTT	4505	0.0	41.607105	1
AACGGAC	60	3.6379788E-12	40.333336	38
CGTTAGG	170	0.0	40.11765	2
GCGATCA	110	0.0	40.0	9
GCTACGA	55	7.8216544E-11	40.0	10
ATGGGCG	430	0.0	39.906975	5
TATGCCA	160	0.0	39.875004	10
TTTACGG	90	0.0	39.111107	2
TGATGCG	90	0.0	39.111107	1
TAACGGG	265	0.0	39.018867	3
ACGGGTA	85	0.0	38.823532	5
GGCGATA	455	0.0	38.68132	8
ATTGCGG	165	0.0	38.666668	2
CTCACGA	205	0.0	38.634148	24
AGGGCGA	1045	0.0	38.526318	6
GGGCGAT	1945	0.0	38.457584	7
TATGGGC	395	0.0	38.43038	4
>>END_MODULE