FastQCFastQC Report
Sat 18 Jun 2016
SRR3549248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681116
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100450.597519742837496No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA28300.16834055472674103No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATA207.8575214E-444.00000432
TAGCGCA207.8575214E-444.00000440
TAAATCG207.8575214E-444.00000434
AACGTAC302.528419E-644.032
TAATCCG254.4437224E-544.01
TCGATGG1100.042.02
TCACGAC3800.041.6842125
CGGTCTA3750.041.6533331
CGTTTTT68250.041.4857141
CTCACGA3900.040.61538324
CGCATCG603.6379788E-1240.33333221
CGTTGAT4550.040.13186625
CGACGGT3950.039.54430428
ACGGTCT4200.038.76190630
TAGGGTA8850.038.531075
AGCGTGA404.12605E-738.50000440
GCGATCG404.12605E-738.5000049
TTACGAG1550.038.3225821
TACGTAG1150.038.260871
AGGGCGC5300.038.188686
Produced by FastQC (version 0.11.5)