##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549247_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2383390
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.315162017126866	31.0	31.0	33.0	30.0	34.0
2	31.699167572239542	31.0	31.0	34.0	30.0	34.0
3	31.83579649155195	31.0	31.0	34.0	30.0	34.0
4	35.52357692194731	37.0	35.0	37.0	33.0	37.0
5	35.403355304838904	37.0	35.0	37.0	33.0	37.0
6	35.44019358980276	37.0	35.0	37.0	33.0	37.0
7	35.94753019858269	37.0	35.0	37.0	35.0	37.0
8	35.94171663051368	37.0	35.0	37.0	35.0	37.0
9	37.63772861344555	39.0	37.0	39.0	35.0	39.0
10	36.965768925773794	39.0	37.0	39.0	32.0	39.0
11	36.74447236918842	39.0	35.0	39.0	32.0	39.0
12	36.181578759665854	38.0	35.0	39.0	32.0	39.0
13	35.96641086855278	38.0	35.0	39.0	31.0	39.0
14	37.00983431163175	39.0	35.0	41.0	31.0	41.0
15	37.22709795711151	39.0	35.0	41.0	32.0	41.0
16	37.300959557604926	39.0	35.0	41.0	32.0	41.0
17	37.23676486013619	39.0	35.0	41.0	32.0	41.0
18	37.19143782595379	39.0	35.0	41.0	32.0	41.0
19	37.15738045389131	38.0	35.0	41.0	32.0	41.0
20	36.97084237157998	38.0	35.0	41.0	32.0	41.0
21	36.85357578910711	38.0	35.0	41.0	32.0	41.0
22	36.766198565908226	38.0	35.0	41.0	31.0	41.0
23	36.69241080981291	38.0	35.0	41.0	31.0	41.0
24	36.61361506090065	38.0	35.0	41.0	31.0	41.0
25	36.43916018779973	38.0	35.0	40.0	31.0	41.0
26	36.33586110540029	38.0	35.0	40.0	31.0	41.0
27	36.23208035613139	38.0	35.0	40.0	30.0	41.0
28	36.288314543570294	38.0	35.0	40.0	31.0	41.0
29	36.22509954308779	38.0	35.0	40.0	30.0	41.0
30	36.198536538292096	38.0	35.0	40.0	30.0	41.0
31	36.062120760765126	38.0	35.0	40.0	30.0	41.0
32	35.79537339671644	38.0	35.0	40.0	30.0	41.0
33	35.66754538703275	38.0	35.0	40.0	29.0	41.0
34	35.55953159155656	38.0	35.0	40.0	29.0	41.0
35	35.38136729616219	38.0	34.0	40.0	27.0	41.0
36	35.19560122346741	38.0	34.0	40.0	26.0	41.0
37	35.146246732595166	38.0	34.0	40.0	26.0	41.0
38	35.11348960933796	37.0	34.0	40.0	27.0	41.0
39	34.989519130314385	37.0	34.0	40.0	26.0	41.0
40	34.87250596838956	37.0	34.0	40.0	25.0	41.0
41	34.86565773960619	37.0	34.0	40.0	25.0	41.0
42	34.68540146597913	37.0	34.0	40.0	24.0	41.0
43	34.69528570649369	37.0	34.0	40.0	25.0	41.0
44	34.43883753812847	36.0	34.0	40.0	24.0	41.0
45	34.36045464653288	36.0	33.0	40.0	23.0	41.0
46	34.358442806254956	36.0	34.0	40.0	24.0	41.0
47	34.24075665333831	36.0	33.0	40.0	23.0	41.0
48	34.20254385560064	36.0	33.0	40.0	23.0	41.0
49	34.173176022388276	36.0	33.0	40.0	24.0	41.0
50	33.597743969723794	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	5.0
13	12.0
14	15.0
15	60.0
16	176.0
17	451.0
18	994.0
19	2069.0
20	3536.0
21	5749.0
22	8842.0
23	12907.0
24	18816.0
25	26727.0
26	36484.0
27	44832.0
28	48730.0
29	53452.0
30	61854.0
31	74230.0
32	92416.0
33	117322.0
34	190841.0
35	306617.0
36	181688.0
37	224117.0
38	321566.0
39	548876.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.2866211572592	24.324009079504403	28.351214027079074	14.038155736157323
2	32.5577014252808	25.39001170601538	28.829776075254156	13.222510793449665
3	28.66610164513571	25.373858243929863	32.15772492122565	13.802315189708775
4	25.370124066980225	28.047067412383203	31.697372230310606	14.885436290325964
5	22.80952760563735	31.962876407134377	30.491694603065383	14.735901384162894
6	19.408531545403815	41.688603208035616	27.956104540171772	10.9467607063888
7	85.93331347366566	3.947780262567184	7.917671887521556	2.2012343762456
8	85.46696092540456	3.2718522776381542	7.58776364757761	3.6734231493796647
9	79.62993886858634	5.464191760475625	10.92221583542769	3.9836535355103444
10	42.70547413557999	26.822970642656045	16.561913912536344	13.909641309227613
11	37.599553577047814	23.173840621971227	23.97652083796609	15.250084963014865
12	33.932507898413604	20.730430185576008	27.723326857962817	17.61373505804757
13	22.459438027347602	32.28023949080931	28.62376698735834	16.636555494484746
14	18.103667465249078	34.929868800322225	28.656493481973154	18.309970252455535
15	16.90566797712502	23.088248251440174	42.115767876847684	17.890315894587122
16	17.741997742711014	20.088025879104972	41.13422478066955	21.035751597514466
17	17.911462244953615	21.476342520527485	30.685410276958454	29.92678495756045
18	21.64681399183516	23.547761801467658	33.272062062860044	21.533362143837138
19	26.661981463377792	27.330860664851325	25.291916136259694	20.715241735511185
20	28.163749952798323	24.27852764339869	25.347047692572346	22.21067471123064
21	23.04633316410659	29.394014408049042	26.51202698677094	21.04762544107343
22	22.38785930963879	25.14284275758478	25.542273820063023	26.927024112713404
23	20.36792132215038	30.151087316805054	25.06526418252993	24.415727178514636
24	20.494379853905574	25.250924103902424	33.94266150315307	20.31203453903893
25	20.070613705688118	26.300983053549775	31.235551042842335	22.39285219791977
26	18.875970781114297	29.93341417057217	27.964915519491147	23.225699528822393
27	21.028325200659566	27.631692673041343	28.131317157494156	23.20866496880494
28	19.506920814470146	27.20003860048083	33.73270845308573	19.560332131963296
29	19.998069975958614	24.233046207292976	31.520229588946837	24.248654227801577
30	22.367552100159855	27.40462114886779	29.191529711880975	21.03629703909138
31	28.2302518681374	25.39941008395605	23.4065763471358	22.963761700770753
32	29.115587461556856	26.47027972761487	23.6310884916023	20.783044319225976
33	27.348608494623207	25.909104259059575	24.687021427462565	22.055265818854657
34	21.734126601185704	27.684810291223844	27.164836640247714	23.416226467342735
35	22.157934706447538	25.26531537012407	29.08621753049228	23.49053239293611
36	27.053524601512972	27.557722403802988	25.391941730056764	19.996811264627272
37	21.972946097785087	29.506291458804473	27.472591560760094	21.048170882650343
38	22.403844943546797	30.62377537876722	25.23351193048557	21.738867747200416
39	21.71092435564469	28.52000721661163	26.3833866887081	23.385681739035576
40	24.183243195616328	25.196463860299822	27.413390171142783	23.206902772941064
41	19.484096182328532	23.637927489835906	28.395352837764698	28.482623490070864
42	23.18630186415148	26.24912414669861	24.697133075157655	25.867440913992258
43	22.554302904686182	25.17162529002807	27.569092762829417	24.704979042456333
44	22.132005253021955	28.420233365080833	27.837953503203423	21.609807878693793
45	19.685699780564658	29.914281758335814	26.489663882117487	23.910354578982037
46	23.620976843907208	28.181078212126426	26.631772391425656	21.566172552540706
47	22.630538854320946	25.741192167458955	27.725802323581117	23.90246665463898
48	23.57654433391094	23.479791389575354	28.496637142893107	24.4470271336206
49	21.921296976155812	23.455624132013643	29.169921834026326	25.45315705780422
50	20.62184535472583	26.933065927103833	29.130230470044765	23.31485824812557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2448.0
1	2703.5
2	2959.0
3	14236.0
4	25513.0
5	18021.5
6	10530.0
7	10803.0
8	11076.0
9	11662.5
10	12249.0
11	12594.0
12	12939.0
13	12464.0
14	11989.0
15	11631.5
16	11274.0
17	10672.5
18	10071.0
19	9723.5
20	9376.0
21	9576.5
22	9777.0
23	9539.0
24	9301.0
25	10648.5
26	11996.0
27	14991.5
28	17987.0
29	21091.0
30	24195.0
31	29342.0
32	34489.0
33	36686.0
34	38883.0
35	45610.5
36	52338.0
37	59434.0
38	66530.0
39	77662.5
40	88795.0
41	117151.0
42	145507.0
43	166835.5
44	188164.0
45	203316.0
46	218468.0
47	228958.0
48	239448.0
49	237345.0
50	235242.0
51	207983.5
52	180725.0
53	158732.5
54	136740.0
55	124254.0
56	111768.0
57	106593.5
58	101419.0
59	95449.5
60	89480.0
61	84323.0
62	79166.0
63	68535.5
64	57905.0
65	48199.0
66	38493.0
67	33045.0
68	27597.0
69	24391.0
70	21185.0
71	18375.0
72	15565.0
73	12365.5
74	9166.0
75	7506.5
76	5847.0
77	4687.5
78	3528.0
79	2745.0
80	1962.0
81	1400.5
82	839.0
83	563.5
84	288.0
85	196.0
86	104.0
87	76.5
88	49.0
89	28.5
90	8.0
91	7.5
92	7.0
93	4.0
94	1.0
95	2.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2383390.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.40562111391821
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.50111848279795	19.158685547099687
2	7.85423136884494	3.833747898581616
3	2.8240871821783853	2.0677080528275575
4	1.5042267192081846	1.4684634951370876
5	0.9944390561584369	1.2134951412742623
6	0.7658016392429924	1.1213918793469173
7	0.581703927245943	0.9937791954189913
8	0.4719623799116369	0.9214828019317228
9	0.3809025177817771	0.8366546277287591
>10	4.177822043524369	23.486653832209967
>50	1.377935117682328	23.96279285604733
>100	0.5586650087662755	18.77050458159674
>500	0.005371737960078582	0.8500816736379566
>1k	0.0015595368271195884	0.6343577485980473
>5k	0.0	0.0
>10k+	1.7328186967995426E-4	0.6802006685633996
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16084	0.6748371017752025	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2733	0.11466860228498063	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06444602016455553	0.0
2	0.0	0.0	0.0	0.2429312869484222	0.0
3	0.0	0.0	0.0	0.43790567217282944	0.0
4	0.0	0.0	0.0	0.6616206327961434	0.0
5	0.0	0.0	0.0	1.091680337670293	0.0
6	0.0	0.0	0.0	2.0701186125644564	0.0
7	0.0	0.0	0.0	2.7027469276954252	0.0
8	0.0	0.0	0.0	4.702881190237435	0.0
9	0.0	0.0	0.0	5.892741011752168	0.0
10	0.0	0.0	0.0	7.466298004103399	0.0
11	0.0	0.0	0.0	8.201469335694116	0.0
12	0.0	0.0	0.0	8.95959117055958	0.0
13	4.195704437796584E-5	0.0	0.0	9.3630501092981	0.0
14	4.195704437796584E-5	0.0	0.0	9.545227595987228	0.0
15	4.195704437796584E-5	0.0	0.0	9.695391857815968	0.0
16	4.195704437796584E-5	0.0	0.0	9.976713840370229	0.0
17	4.195704437796584E-5	0.0	0.0	10.265378305690634	0.0
18	8.391408875593168E-5	0.0	0.0	10.776666848480525	0.0
19	8.391408875593168E-5	0.0	0.0	10.957837366104583	0.0
20	8.391408875593168E-5	0.0	0.0	11.196405120437696	0.0
21	8.391408875593168E-5	0.0	0.0	11.435644187480857	0.0
22	8.391408875593168E-5	0.0	0.0	11.673330843882034	0.0
23	8.391408875593168E-5	0.0	0.0	11.964596645953872	0.0
24	8.391408875593168E-5	0.0	0.0	12.182395663319893	0.0
25	8.391408875593168E-5	0.0	0.0	12.373719785683416	0.0
26	8.391408875593168E-5	0.0	0.0	12.550275028425897	0.0
27	8.391408875593168E-5	0.0	0.0	12.746466167937266	0.0
28	8.391408875593168E-5	0.0	0.0	12.933342843596726	0.0
29	8.391408875593168E-5	0.0	0.0	13.127939615421731	0.0
30	8.391408875593168E-5	0.0	0.0	13.411485321328025	0.0
31	8.391408875593168E-5	0.0	0.0	13.618753120555176	0.0
32	8.391408875593168E-5	0.0	0.0	13.826902017714264	0.0
33	8.391408875593168E-5	0.0	0.0	14.030519554080533	0.0
34	8.391408875593168E-5	0.0	0.0	14.224948497728025	0.0
35	8.391408875593168E-5	0.0	0.0	14.467879784676448	0.0
36	8.391408875593168E-5	0.0	0.0	14.674853884592954	0.0
37	8.391408875593168E-5	0.0	0.0	14.89168788993828	0.0
38	8.391408875593168E-5	0.0	0.0	15.121025094508243	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAT	20	7.858185E-4	44.0	11
TACGCTA	25	4.4442837E-5	44.0	1
TCGCTAA	25	4.4442837E-5	44.0	27
CGTCAAT	25	4.4442837E-5	44.0	33
CGTTTTT	10390	0.0	42.284885	1
CGGTCTA	700	0.0	41.485714	31
CGACGGT	715	0.0	40.923077	28
TACGCGG	170	0.0	40.11765	2
CACGACG	735	0.0	40.10884	26
TCACGAC	755	0.0	39.629143	25
AGGGTAC	870	0.0	39.195404	6
TCGATAG	90	0.0	39.11111	1
GTCGTAA	45	2.3526809E-8	39.11111	27
TACGGGA	580	0.0	38.689655	4
CGGTTTA	40	4.127014E-7	38.5	38
CGGCATA	40	4.127014E-7	38.5	13
ACGGTCT	755	0.0	38.463577	30
GACGGTC	765	0.0	38.248367	29
TTTAGCG	150	0.0	38.13333	1
ACGTATC	35	7.293498E-6	37.714287	10
>>END_MODULE