##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549240_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	940187
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.479379102242426	31.0	31.0	33.0	30.0	34.0
2	31.896929015185275	31.0	31.0	34.0	30.0	34.0
3	32.0210936760453	33.0	31.0	34.0	30.0	34.0
4	35.73160339379294	37.0	35.0	37.0	33.0	37.0
5	35.62514372140861	37.0	35.0	37.0	33.0	37.0
6	35.69588922203774	37.0	35.0	37.0	33.0	37.0
7	36.04128221300656	37.0	35.0	37.0	35.0	37.0
8	36.02596079290609	37.0	35.0	37.0	35.0	37.0
9	37.78103717664678	39.0	38.0	39.0	35.0	39.0
10	37.269890989771184	39.0	37.0	39.0	34.0	39.0
11	36.94591607839717	39.0	37.0	39.0	33.0	39.0
12	36.16890363300067	38.0	35.0	39.0	32.0	39.0
13	35.85823990333838	38.0	35.0	39.0	31.0	39.0
14	36.88532068620391	39.0	35.0	41.0	31.0	41.0
15	37.187038323227185	39.0	35.0	41.0	32.0	41.0
16	37.30445964473025	39.0	35.0	41.0	33.0	41.0
17	37.25207006691222	39.0	35.0	41.0	32.0	41.0
18	37.18636398929149	39.0	35.0	41.0	32.0	41.0
19	37.13631968959366	38.0	35.0	41.0	32.0	41.0
20	36.957182985937905	38.0	35.0	41.0	32.0	41.0
21	36.826370711358486	38.0	35.0	41.0	32.0	41.0
22	36.72389854358761	38.0	35.0	41.0	31.0	41.0
23	36.715219419115556	38.0	35.0	41.0	32.0	41.0
24	36.62199115707833	38.0	35.0	41.0	31.0	41.0
25	36.57293283144736	38.0	35.0	41.0	31.0	41.0
26	36.425795081191296	38.0	35.0	40.0	31.0	41.0
27	36.37163457907842	38.0	35.0	40.0	31.0	41.0
28	36.33914742492717	38.0	35.0	40.0	31.0	41.0
29	36.42308923650295	38.0	35.0	40.0	31.0	41.0
30	36.40398665371889	38.0	35.0	40.0	31.0	41.0
31	36.325799016578614	38.0	35.0	40.0	31.0	41.0
32	36.18220524214863	38.0	35.0	40.0	30.0	41.0
33	36.095250200226126	38.0	35.0	41.0	30.0	41.0
34	36.02454086261563	38.0	35.0	41.0	30.0	41.0
35	35.96182142488675	38.0	35.0	41.0	30.0	41.0
36	35.832269537868534	38.0	35.0	41.0	30.0	41.0
37	35.79850178741038	38.0	35.0	41.0	30.0	41.0
38	35.76397567717911	38.0	35.0	40.0	30.0	41.0
39	35.749660439891215	38.0	35.0	40.0	30.0	41.0
40	35.60246099977983	38.0	35.0	40.0	29.0	41.0
41	35.608809736786405	37.0	35.0	40.0	29.0	41.0
42	35.54662423539147	37.0	35.0	40.0	29.0	41.0
43	35.48641387298484	37.0	35.0	40.0	29.0	41.0
44	35.367140792204104	37.0	34.0	40.0	29.0	41.0
45	35.26555461838974	37.0	34.0	40.0	28.0	41.0
46	35.23426509832618	37.0	34.0	40.0	28.0	41.0
47	35.232125098517635	37.0	34.0	40.0	28.0	41.0
48	35.15365134808288	36.0	34.0	40.0	28.0	41.0
49	35.11039825056079	36.0	34.0	40.0	28.0	41.0
50	35.002490993812934	36.0	34.0	40.0	28.0	41.0
51	34.628981255856544	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	0.0
12	2.0
13	4.0
14	7.0
15	21.0
16	44.0
17	110.0
18	317.0
19	582.0
20	1008.0
21	1668.0
22	2655.0
23	4076.0
24	5931.0
25	8461.0
26	11679.0
27	14425.0
28	15692.0
29	18410.0
30	21269.0
31	26335.0
32	33539.0
33	44157.0
34	79761.0
35	140274.0
36	60293.0
37	76624.0
38	118119.0
39	254361.0
40	361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.24698916279421	24.686259222899274	29.891819393376	13.174932220930517
2	30.414694098088997	27.138005524432906	29.28651427854246	13.160786098935636
3	30.85960558910089	25.02651068351296	31.14720794905694	12.966675778329204
4	28.992530209415783	27.454963746573817	28.830647520121	14.721858523889397
5	24.30367575812046	32.50066210232645	29.25460573268935	13.941056406863742
6	21.82682806718238	42.964325182118024	25.490886387495255	9.717960363204341
7	86.99194947388126	4.989539315051155	6.205999444791302	1.8125117662762833
8	88.03854977786334	4.27723420979018	5.981150558346371	1.7030654540001084
9	82.66866059624309	7.904491340552465	7.083484455751887	2.34336360745256
10	48.73136939779001	30.527543988589507	11.257228615158473	9.483857998462009
11	43.729598473495166	22.216218688409857	21.07080825410264	12.983374583992333
12	40.92855995668947	22.44702383674737	23.161987987496104	13.462428219067057
13	22.958411464953247	37.36139725395054	23.875037625493654	15.805153655602556
14	15.079021513805232	40.58150133962712	27.154385244637503	17.185091901930146
15	16.338558180447084	27.1809756995151	42.00292069556376	14.477545424474068
16	18.02152125055973	23.35609830810254	41.594916755921965	17.02746368541577
17	18.099697187899856	24.366003784353538	27.57961979903998	29.95467922870663
18	22.331621262578615	28.10930166020164	30.737183134844454	18.821893942375294
19	29.95223290685789	27.19682361062214	24.442052485303456	18.408890997216513
20	31.079668193667853	26.59907018497384	25.203603112997737	17.11765850836057
21	24.1215843231187	30.024984391402988	26.90369043605155	18.949740849426764
22	23.258458157792013	24.523632000867913	25.985894295496536	26.23201554584354
23	22.249722661555627	29.310871135210338	26.37400857488989	22.06539762834415
24	20.278838146028395	26.640551294582888	36.136321816830055	16.944288742558662
25	18.776583807263876	29.314381075254175	32.480027909341445	19.429007208140508
26	17.524705191626772	37.185900251758426	26.117889313508908	19.171505243105894
27	19.681935614936176	34.851151951686205	28.393925889211403	17.072986544166213
28	15.464264024071808	31.355464391658256	37.037631875360965	16.142639708908973
29	16.561279830501803	27.883282793742097	35.18661713042193	20.368820245334174
30	18.82933926974102	33.50110137664103	29.405214069116038	18.26434528450191
31	30.57413046553505	27.406994565974642	24.031708585632433	17.98716638285788
32	28.16365254997144	30.3839555322505	25.0904341370387	16.361957780739363
33	26.22605928395096	31.056694040653614	24.01001077445232	18.70723590094311
34	19.41220204065787	30.267276616247617	27.220223210914423	23.10029813218009
35	22.6626192448949	27.00324509911326	28.679613736416265	21.654521919575572
36	28.42477081686941	29.09506300342379	25.333577256439412	17.14658892326739
37	20.707050831377163	34.72319868281522	27.08525006195576	17.484500423851852
38	20.09153498187063	34.770423330677836	24.038303018442075	21.09973866900946
39	22.701122223557654	31.166884885666363	26.168304815956827	19.963688074819157
40	23.76154956407608	28.937328425089902	26.280516535540272	21.020605475293745
41	18.252751846175283	26.125547364513658	29.1136763218381	26.508024467472964
42	21.121755565648108	27.985709225930588	24.652436164294976	26.24009904412633
43	23.16656154573505	26.411341573538028	25.58310208501075	24.83899479571617
44	19.217134463675844	31.8829126546102	28.35127479958774	20.548678082126216
45	19.285737837260033	37.8745930330881	23.108275268643368	19.7313938610085
46	21.897345953517757	35.04153960860978	24.223053498931595	18.83806093894087
47	22.76228026977612	28.2934139697741	26.642784892792605	22.301520867657178
48	22.14506263115742	26.822430005945623	31.78133711697779	19.251170245919162
49	21.032092551800865	27.997196302437704	30.840035014310985	20.130676131450446
50	18.990477426299236	33.20031015106569	28.784592852273004	19.024619570362066
51	20.0215489046328	33.059061654755915	25.534388371675	21.385001068936287
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	955.0
1	1565.0
2	2175.0
3	4848.0
4	7521.0
5	5581.5
6	3642.0
7	3582.0
8	3522.0
9	3662.0
10	3802.0
11	3617.5
12	3433.0
13	3253.5
14	3074.0
15	2981.5
16	2889.0
17	2761.0
18	2633.0
19	2802.5
20	2972.0
21	3020.5
22	3069.0
23	3935.5
24	4802.0
25	5794.5
26	9453.0
27	12119.0
28	16857.0
29	21595.0
30	24636.0
31	27677.0
32	29135.5
33	30594.0
34	30661.0
35	30728.0
36	33086.0
37	35444.0
38	36277.5
39	37111.0
40	52102.5
41	67094.0
42	76326.5
43	85559.0
44	88004.5
45	90450.0
46	86206.0
47	81962.0
48	77717.5
49	73473.0
50	63568.5
51	53664.0
52	49978.0
53	46292.0
54	42189.5
55	38087.0
56	36079.5
57	34072.0
58	33470.5
59	32869.0
60	31161.0
61	29453.0
62	25434.0
63	21415.0
64	18092.5
65	14770.0
66	12310.0
67	9850.0
68	7812.0
69	5774.0
70	4749.5
71	3725.0
72	3145.0
73	2565.0
74	1938.0
75	979.0
76	647.0
77	452.5
78	258.0
79	200.5
80	143.0
81	164.5
82	186.0
83	97.5
84	9.0
85	5.5
86	2.0
87	2.0
88	2.0
89	2.5
90	3.0
91	4.0
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	940187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.049697333354572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.14472892583079	14.646377973083476
2	6.398171692943386	2.309701250889299
3	1.8709070709026192	1.0130791910587567
4	0.9308163336545031	0.6720381238162626
5	0.6191451093623924	0.558769091470895
6	0.4464111363755341	0.48345515386903565
7	0.3672359774623968	0.4639948770180418
8	0.28884401025580214	0.4170837569335674
9	0.23975490912384262	0.38947531894841614
>10	4.529261249989916	21.184127666249783
>50	2.026252796635562	26.064457507852968
>100	1.1236546658148656	28.651220667398647
>500	0.0112602524527861	1.4744828132982244
>1k	0.0035558691956166628	1.6717366081126412
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4005	0.4259790871390478	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3891	0.4138538397148652	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3295	0.3504621952866824	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2202	0.23420872656184352	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1175	0.12497513792468944	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1060	0.11274352868099645	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	981	0.10434094493967688	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026271369419062378	0.0
2	0.0	0.0	0.0	0.09753378849101296	0.0
3	0.0	0.0	0.0	0.14869382367550285	0.0
4	0.0	0.0	0.0	0.2475039540006403	0.0
5	0.0	0.0	0.0	0.44820870741671603	0.0
6	0.0	0.0	0.0	0.621684835038136	0.0
7	0.0	0.0	0.0	0.726344865436344	0.0
8	0.0	0.0	0.0	0.9389621426375817	0.0
9	0.0	0.0	0.0	1.00107744523164	0.0
10	0.0	0.0	0.0	1.1277543722684955	0.0
11	0.0	0.0	0.0	1.3200565419432517	0.0
12	0.0	0.0	0.0	1.4813010603209786	0.0
13	0.0	0.0	0.0	1.5410764028858088	0.0
14	0.0	0.0	0.0	1.565858706831726	0.0
15	0.0	0.0	0.0	1.6015962781872117	0.0
16	0.0	0.0	0.0	1.6900893120198428	0.0
17	0.0	0.0	0.0	1.795919322432665	0.0
18	0.0	0.0	0.0	1.936104200547338	0.0
19	0.0	0.0	0.0	2.0125783487752966	0.0
20	0.0	0.0	0.0	2.096497824368982	0.0
21	0.0	0.0	0.0	2.2127512930938207	0.0
22	0.0	0.0	0.0	2.332301978223481	0.0
23	0.0	0.0	0.0	2.4657860617090006	0.0
24	0.0	0.0	0.0	2.5568317792098805	0.0
25	0.0	0.0	0.0	2.645431174862022	0.0
26	1.06361819510374E-4	0.0	0.0	2.733605123236122	0.0
27	1.06361819510374E-4	0.0	0.0	2.8135892115079235	0.0
28	1.06361819510374E-4	0.0	0.0	2.908463954511177	0.0
29	1.06361819510374E-4	0.0	0.0	3.0073804466558247	0.0
30	1.06361819510374E-4	0.0	0.0	3.12257029718556	0.0
31	1.06361819510374E-4	0.0	0.0	3.2449927514420005	0.0
32	1.06361819510374E-4	0.0	0.0	3.363479818376557	0.0
33	2.12723639020748E-4	0.0	0.0	3.471224341540566	0.0
34	2.12723639020748E-4	0.0	0.0	3.5886477902800187	0.0
35	2.12723639020748E-4	0.0	0.0	3.7491477759211733	0.0
36	2.12723639020748E-4	0.0	0.0	3.8849718194359206	0.0
37	2.12723639020748E-4	0.0	0.0	4.016541390170254	0.0
38	2.12723639020748E-4	0.0	0.0	4.145026468138785	0.0
39	2.12723639020748E-4	0.0	0.0	4.281488682570595	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	20	7.03263E-4	45.000004	43
ACTATCG	20	7.03263E-4	45.000004	1
GATACCA	35	1.2116107E-7	45.000004	20
GGTACGA	20	7.03263E-4	45.000004	9
GTCGATA	20	7.03263E-4	45.000004	25
CAACGCG	20	7.03263E-4	45.000004	14
TAACGTA	20	7.03263E-4	45.000004	34
GTTACAT	20	7.03263E-4	45.000004	8
CTAACGT	20	7.03263E-4	45.000004	33
CATAGGT	30	2.1650776E-6	45.000004	35
CTACGGC	20	7.03263E-4	45.000004	41
ACTACGG	20	7.03263E-4	45.000004	40
CGAATAT	30	2.1650776E-6	45.000004	14
GATGCGA	40	6.8121153E-9	45.000004	9
CATACGC	35	1.2116107E-7	45.000004	32
ACAGACG	20	7.03263E-4	45.000004	11
CCAACGG	30	2.1650776E-6	45.000004	2
CACGTGC	20	7.03263E-4	45.000004	42
CACAACG	20	7.03263E-4	45.000004	12
CGATCAA	20	7.03263E-4	45.000004	10
>>END_MODULE