FastQCFastQC Report
Sat 18 Jun 2016
SRR3549239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532613
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106150.6926079838811233No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT17390.11346634799522122No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78150.042.9558531
TGTAACG1050.042.8571431
TACGGGA3700.040.7432444
TAATCGT3450.040.4347821
CACAACG2950.040.4237312
TGCGTAG1350.040.01
CGAAAGG2400.039.3749962
TCGTTGA3550.039.29577624
TTAATCG3600.038.7520
ACGGGAT4100.038.4146355
CGTTAGG2150.037.6744162
CATACGA5750.037.56521618
TCGAGTA301.1400999E-437.49999642
TAGGGAT16150.037.337465
GCGTTAG1150.037.1739161
TAAGGGA16650.037.1621634
AGGGATC10600.037.1462256
ATAGGGA18250.037.1095924
CGTTGAT3700.037.09459325
GTTGATC7650.037.05882616
Produced by FastQC (version 0.11.5)