##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549237_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1316255
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.716658246312456	31.0	31.0	34.0	30.0	34.0
2	32.050232667682174	33.0	31.0	34.0	30.0	34.0
3	32.09857208519626	33.0	31.0	34.0	30.0	34.0
4	35.83130472438851	37.0	35.0	37.0	35.0	37.0
5	35.79127144816164	37.0	35.0	37.0	35.0	37.0
6	35.83682948972653	37.0	35.0	37.0	35.0	37.0
7	36.07342574197249	37.0	35.0	37.0	35.0	37.0
8	13.224322034864064	0.0	0.0	35.0	0.0	37.0
9	24.89427960387615	18.0	17.0	38.0	17.0	39.0
10	32.66585198156892	32.0	32.0	38.0	27.0	39.0
11	35.5820103247471	37.0	35.0	37.0	32.0	39.0
12	36.463240025678914	37.0	35.0	39.0	33.0	39.0
13	36.528194004961044	39.0	35.0	39.0	33.0	39.0
14	37.69141845615022	39.0	36.0	41.0	33.0	41.0
15	38.063195201537695	40.0	36.0	41.0	33.0	41.0
16	38.21803981751256	40.0	36.0	41.0	34.0	41.0
17	38.20997679021162	40.0	36.0	41.0	34.0	41.0
18	38.156721911787606	40.0	36.0	41.0	34.0	41.0
19	38.12817653114328	40.0	36.0	41.0	34.0	41.0
20	38.085510026552605	40.0	36.0	41.0	34.0	41.0
21	38.00503397897824	40.0	36.0	41.0	34.0	41.0
22	37.93747943977421	40.0	36.0	41.0	33.0	41.0
23	37.84690276580146	40.0	36.0	41.0	33.0	41.0
24	37.77903141868407	40.0	35.0	41.0	33.0	41.0
25	37.647195262316195	40.0	35.0	41.0	33.0	41.0
26	37.546348541885884	40.0	35.0	41.0	33.0	41.0
27	37.47239174779963	39.0	35.0	41.0	33.0	41.0
28	37.454898177024965	40.0	35.0	41.0	33.0	41.0
29	37.518583405191244	40.0	35.0	41.0	33.0	41.0
30	37.45387557882022	40.0	35.0	41.0	33.0	41.0
31	37.34563667374483	40.0	35.0	41.0	33.0	41.0
32	37.19376184705851	40.0	35.0	41.0	32.0	41.0
33	37.081225902275776	40.0	35.0	41.0	32.0	41.0
34	36.992291007441565	40.0	35.0	41.0	32.0	41.0
35	36.9119760228831	40.0	35.0	41.0	31.0	41.0
36	36.8075661630915	40.0	35.0	41.0	31.0	41.0
37	36.72553266654258	39.0	35.0	41.0	31.0	41.0
38	36.66768369350924	39.0	35.0	41.0	31.0	41.0
39	36.61978112143923	39.0	35.0	41.0	31.0	41.0
40	36.47625422125652	39.0	35.0	41.0	30.0	41.0
41	36.45448564297951	39.0	35.0	41.0	31.0	41.0
42	36.40397415394433	39.0	35.0	41.0	30.0	41.0
43	36.2993796794694	39.0	35.0	41.0	30.0	41.0
44	36.117247037997956	39.0	35.0	40.0	30.0	41.0
45	36.0154324200098	38.0	35.0	40.0	30.0	41.0
46	36.00767252546049	38.0	35.0	40.0	30.0	41.0
47	35.95957242327665	38.0	35.0	40.0	30.0	41.0
48	35.90412344112653	38.0	35.0	40.0	30.0	41.0
49	35.83789691207251	38.0	35.0	40.0	29.0	41.0
50	35.702178529236356	38.0	35.0	40.0	29.0	41.0
51	35.3196918530224	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	7.0
14	10.0
15	10.0
16	54.0
17	130.0
18	363.0
19	654.0
20	1258.0
21	2114.0
22	3448.0
23	5270.0
24	7739.0
25	11067.0
26	14300.0
27	16372.0
28	17853.0
29	20838.0
30	26204.0
31	34438.0
32	45155.0
33	63043.0
34	101089.0
35	160187.0
36	133995.0
37	225353.0
38	313811.0
39	111451.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.00093446938473	23.60925504556488	29.23969899449575	13.150111490554641
2	29.647066867742193	26.70835058556283	31.009644787674123	12.63493775902086
3	30.340093674857837	25.086096539044483	31.433498828114615	13.140310957983065
4	27.392412564434704	27.172926218703825	30.871677600464952	14.562983616396519
5	23.8858731780696	31.834560932342136	29.05979464465472	15.219771244933542
6	21.70916729661046	41.00732760749246	26.348541885880778	10.934963210016296
7	85.82668251972451	5.121424040174586	6.888634801007404	2.1632586390934887
8	32.33180500738839	64.01343204774152	2.3678542531652305	1.2869086917048749
9	81.75771412074408	6.847913208306901	7.870207520579219	3.5241651503697993
10	53.02809865869455	24.180117074579012	12.325423265248755	10.466361001477678
11	48.49098388989975	19.91612567473628	18.45432685915723	13.138563576206739
12	42.92412944300307	21.742063657877843	22.031977086506792	13.301829812612297
13	21.45982351444059	41.18905531223053	22.752810055802257	14.598311117526618
14	14.54786496537525	43.30293142286259	26.45148546444268	15.697718147319478
15	14.411873079304542	23.87751613479151	47.965249894587295	13.745360891316652
16	15.764042681699214	19.398976642064035	45.645334680590004	19.191645995646738
17	16.038381620582637	20.379561711066625	27.56532738717042	36.01672928118032
18	21.529870731735112	24.74892782933398	33.05446133158089	20.66674010735002
19	29.47901432473191	26.0331014886933	23.959263212675356	20.528620973899432
20	31.40622447777976	24.413278582037677	23.921808464165377	20.258688476017188
21	23.241165275725447	29.544883020387385	26.456043851685273	20.75790785220189
22	22.490702789353126	25.329590390919694	24.404161807552487	27.77554501217469
23	21.055190673539702	30.276656119065077	24.504978138734515	24.16317506866071
24	20.982560370141044	24.70775039790922	36.76430478896566	17.545384442984073
25	18.472180542524054	25.53608533300918	33.871020432970816	22.12071369149595
26	16.976725634470526	35.18474763628628	26.114620647215013	21.723906082028176
27	19.51035323702474	32.98357840995856	28.677345955001122	18.828722398015582
28	14.597171520715971	29.756316215323018	38.08038715902314	17.566125104937875
29	16.1932148405894	25.421441893857953	36.46603431705863	21.919308948494024
30	19.411208314498328	29.98765436788464	30.47836475454984	20.122772563067187
31	30.596123091650174	25.93988246958226	23.01203034366441	20.451964095103154
32	30.060740510007562	27.64016091106966	25.056467021967627	17.24263155695515
33	26.288675066761378	27.35898439132235	25.257643845607426	21.094696696308844
34	18.319702489259303	28.16445141708863	28.18450832095605	25.33133777269602
35	19.717759856562747	24.993713224261256	31.132037485137758	24.156489434038235
36	29.082966446471236	25.544670295649397	26.541893478087452	18.83046977979191
37	20.003190871069815	31.9970674375406	28.051099520989474	19.948642170400113
38	19.638671837903747	33.381677562478394	23.655066837352944	23.32458376226491
39	21.42366030898268	29.76664855973957	26.58542607625422	22.224265055023533
40	23.40116466794048	26.077013952463616	26.092892334691985	24.428929044903914
41	17.315413806595224	24.643097272185102	27.221245123475313	30.820243797744357
42	22.09966913705931	25.96776460488279	24.313146008942034	27.619420249115862
43	22.751670458991608	25.688791305636066	25.3956870059373	26.163851229435025
44	20.253598276929623	30.495306760468143	27.08274612442118	22.16834883818105
45	18.059494550827917	36.148162780008434	22.626618702303126	23.165723966860526
46	22.904452404739203	31.500659066822156	25.767651404932934	19.827237123505704
47	22.51600183854952	26.325522030305677	27.220257472906084	23.938218658238718
48	22.63687507359896	23.65020455762751	30.63479341009151	23.07812695868202
49	19.989135843738485	23.968228040919122	31.94722907035491	24.095407044987482
50	18.880915931943278	31.680943282266732	27.950739028531707	21.487401757258283
51	18.65406019350354	32.42882268253492	24.77916513137652	24.137951992585023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1514.0
1	2084.5
2	2655.0
3	6180.0
4	9705.0
5	7240.0
6	4775.0
7	4905.5
8	5036.0
9	5312.5
10	5589.0
11	5552.5
12	5516.0
13	5401.0
14	5286.0
15	5021.0
16	4756.0
17	4510.5
18	4265.0
19	4312.5
20	4360.0
21	4397.5
22	4435.0
23	5020.0
24	5605.0
25	7428.5
26	11962.5
27	14673.0
28	17215.0
29	19757.0
30	21169.5
31	22582.0
32	24129.0
33	25676.0
34	27299.5
35	28923.0
36	33519.0
37	38115.0
38	54830.5
39	71546.0
40	91901.0
41	112256.0
42	121256.5
43	130257.0
44	129643.0
45	129029.0
46	126030.5
47	123032.0
48	116948.5
49	110865.0
50	99762.5
51	88660.0
52	79249.5
53	69839.0
54	64025.5
55	58212.0
56	55421.0
57	52630.0
58	48270.0
59	43910.0
60	38870.0
61	33830.0
62	29549.0
63	25268.0
64	21379.0
65	17490.0
66	14109.0
67	10728.0
68	8524.0
69	6320.0
70	5242.0
71	4164.0
72	3337.0
73	2510.0
74	1975.0
75	1139.0
76	838.0
77	664.5
78	491.0
79	361.0
80	231.0
81	194.0
82	157.0
83	108.0
84	59.0
85	33.0
86	7.0
87	6.0
88	5.0
89	4.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1316255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.365981910302466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.72856539494457	16.821131040524502
2	6.429528385697671	2.7474637436118523
3	2.0442477128266314	1.3103207895729296
4	1.1307333299815565	0.96636911495048
5	0.7466846463566743	0.7976825323378648
6	0.5621007829492541	0.720590109615637
7	0.4940095233714721	0.738849897791041
8	0.402422354039469	0.6878518989366904
9	0.3449014876383698	0.6632243051146036
>10	7.009389131889103	38.62004711460752
>50	1.8687536943727183	26.985244070314558
>100	0.23148570462627982	7.115145939882743
>500	0.003230033087808485	0.4327104348441916
>1k	0.003947818218432593	1.393369007895411
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3435	0.2609676696384819	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2138	0.16243053207775088	No Hit
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2122	0.1612149621463926	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1867	0.14184181636536994	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.519462414197857E-4	0.0	0.0	0.03388401183661221	0.0
2	1.519462414197857E-4	0.0	0.0	0.11433954666838872	0.0
3	1.519462414197857E-4	0.0	0.0	0.16113898902568272	0.0
4	1.519462414197857E-4	0.0	0.0	0.25602941679233887	0.0
5	1.519462414197857E-4	0.0	0.0	0.46867818165932895	0.0
6	1.519462414197857E-4	0.0	0.0	0.6370346171524515	0.0
7	1.519462414197857E-4	0.0	0.0	0.7526657068729083	0.0
8	1.519462414197857E-4	0.0	0.0	1.0049724407504625	0.0
9	1.519462414197857E-4	0.0	0.0	1.208732350494395	0.0
10	1.519462414197857E-4	0.0	0.0	1.6850838173454232	0.0
11	1.519462414197857E-4	0.0	0.0	2.0542372108747924	0.0
12	1.519462414197857E-4	0.0	0.0	2.438129389821881	0.0
13	1.519462414197857E-4	0.0	0.0	2.582630265412097	0.0
14	1.519462414197857E-4	0.0	0.0	2.6342919874948243	0.0
15	1.519462414197857E-4	0.0	0.0	2.724775974260307	0.0
16	1.519462414197857E-4	0.0	0.0	2.900349856220869	0.0
17	1.519462414197857E-4	0.0	0.0	3.0871677600464955	0.0
18	1.519462414197857E-4	0.0	0.0	3.404963323975977	0.0
19	1.519462414197857E-4	0.0	0.0	3.5330540054928568	0.0
20	1.519462414197857E-4	0.0	0.0	3.6743640100132575	0.0
21	1.519462414197857E-4	0.0	0.0	3.8636130537016005	0.0
22	1.519462414197857E-4	0.0	0.0	4.040858344317781	0.0
23	1.519462414197857E-4	0.0	0.0	4.252975297339801	0.0
24	1.519462414197857E-4	0.0	0.0	4.4099357647264394	0.0
25	1.519462414197857E-4	0.0	0.0	4.533923897724985	0.0
26	1.519462414197857E-4	0.0	0.0	4.6602671974655365	0.0
27	1.519462414197857E-4	0.0	0.0	4.772707416116178	0.0
28	1.519462414197857E-4	0.0	0.0	4.892365081234259	0.0
29	1.519462414197857E-4	0.0	0.0	5.019999924026879	0.0
30	1.519462414197857E-4	0.0	0.0	5.199296488902227	0.0
31	1.519462414197857E-4	0.0	0.0	5.360131585445069	0.0
32	1.519462414197857E-4	0.0	0.0	5.498174745774945	0.0
33	1.519462414197857E-4	0.0	0.0	5.638345153484697	0.0
34	1.519462414197857E-4	0.0	0.0	5.788316093766025	0.0
35	1.519462414197857E-4	0.0	0.0	5.985238422646068	0.0
36	1.519462414197857E-4	0.0	0.0	6.146225465430331	0.0
37	1.519462414197857E-4	0.0	0.0	6.288827013002799	0.0
38	1.519462414197857E-4	0.0	0.0	6.430061044402491	0.0
39	1.519462414197857E-4	0.0	0.0	6.577145006096843	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGA	150	0.0	45.000004	2
ACTACAT	25	3.891115E-5	45.000004	16
AACGAGT	25	3.891115E-5	45.000004	4
TACTCGC	25	3.891115E-5	45.000004	45
CGTAAGA	75	0.0	45.000004	2
GTAAGCG	90	0.0	45.000004	1
CGATCGA	25	3.891115E-5	45.000004	6
ATCGATC	25	3.891115E-5	45.000004	24
GTACGTA	25	3.891115E-5	45.000004	22
ACGGTTT	25	3.891115E-5	45.000004	11
AACGTGT	20	7.03366E-4	45.0	22
AGCGTAA	20	7.03366E-4	45.0	19
ACTATCG	20	7.03366E-4	45.0	13
CGAACGC	20	7.03366E-4	45.0	24
CAACGTA	20	7.03366E-4	45.0	35
CGACGAA	20	7.03366E-4	45.0	10
ACGTTAG	110	0.0	45.0	1
CGCGATC	20	7.03366E-4	45.0	30
TAAGTCG	20	7.03366E-4	45.0	38
TGCGGTA	20	7.03366E-4	45.0	33
>>END_MODULE