FastQCFastQC Report
Sat 18 Jun 2016
SRR3549235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019811
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27170.26642191543334987No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24590.2411231100664731No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13900.13629976534867735No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGA750.045.02
CAACGGC207.0329127E-445.015
GCGCGTA207.0329127E-445.024
CTATACG207.0329127E-445.01
CCTACGG207.0329127E-445.022
ATTCGAA207.0329127E-445.015
GCACCGT207.0329127E-445.036
TACGCGT207.0329127E-445.013
TACGATT253.890496E-545.016
CGTGTAA207.0329127E-445.034
TACTAGC351.2117562E-745.042
ATACGTG207.0329127E-445.01
CACGTAG253.890496E-545.01
ATACGAT207.0329127E-445.010
CGACTAA253.890496E-545.033
TATCGTG207.0329127E-445.01
AGCGTAT302.1652613E-644.99999631
TATGCGC302.1652613E-644.99999616
AAATTCG302.1652613E-644.99999611
CTACCTA302.1652613E-644.9999962
Produced by FastQC (version 0.11.5)