##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549235_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1019811
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.766531249417785	31.0	31.0	34.0	30.0	34.0
2	32.099690040605566	33.0	31.0	34.0	30.0	34.0
3	32.128971936956944	33.0	31.0	34.0	30.0	34.0
4	35.858274719531366	37.0	35.0	37.0	35.0	37.0
5	35.829915543174174	37.0	35.0	37.0	35.0	37.0
6	35.869394427006576	37.0	35.0	37.0	35.0	37.0
7	36.122486421503595	37.0	35.0	37.0	35.0	37.0
8	13.252575232077316	0.0	0.0	36.0	0.0	37.0
9	24.95862566691279	18.0	17.0	39.0	17.0	39.0
10	32.77500046577258	32.0	32.0	38.0	27.0	39.0
11	35.59027800249262	37.0	35.0	37.0	32.0	39.0
12	36.494766187067995	37.0	35.0	39.0	33.0	39.0
13	36.56664225037777	39.0	35.0	39.0	33.0	39.0
14	37.72388413147142	39.0	36.0	41.0	33.0	41.0
15	38.137457823067216	40.0	37.0	41.0	33.0	41.0
16	38.276706173987144	40.0	37.0	41.0	34.0	41.0
17	38.27201020581265	40.0	36.0	41.0	34.0	41.0
18	38.2286560941194	40.0	36.0	41.0	34.0	41.0
19	38.21081455289264	40.0	36.0	41.0	34.0	41.0
20	38.17389888910788	40.0	36.0	41.0	34.0	41.0
21	38.07672500100509	40.0	36.0	41.0	34.0	41.0
22	38.013623112517905	40.0	36.0	41.0	33.0	41.0
23	37.93326214367172	40.0	36.0	41.0	34.0	41.0
24	37.872129247478206	40.0	35.0	41.0	34.0	41.0
25	37.768027605115066	40.0	35.0	41.0	33.0	41.0
26	37.67525845475289	40.0	35.0	41.0	33.0	41.0
27	37.60108980977848	40.0	35.0	41.0	33.0	41.0
28	37.592864756312686	40.0	35.0	41.0	33.0	41.0
29	37.633384029001455	40.0	35.0	41.0	33.0	41.0
30	37.560830389160344	40.0	36.0	41.0	33.0	41.0
31	37.46604419838578	40.0	35.0	41.0	33.0	41.0
32	37.314225871264384	40.0	35.0	41.0	33.0	41.0
33	37.184918578050244	40.0	35.0	41.0	32.0	41.0
34	37.09407331358457	40.0	35.0	41.0	32.0	41.0
35	37.00363498726725	40.0	35.0	41.0	32.0	41.0
36	36.88551702227178	40.0	35.0	41.0	31.0	41.0
37	36.78944824089954	40.0	35.0	41.0	31.0	41.0
38	36.721827868104974	39.0	35.0	41.0	31.0	41.0
39	36.65523611728055	39.0	35.0	41.0	31.0	41.0
40	36.54750242937172	39.0	35.0	41.0	31.0	41.0
41	36.5345961163392	39.0	35.0	41.0	31.0	41.0
42	36.48377983763658	39.0	35.0	41.0	31.0	41.0
43	36.38054894485351	39.0	35.0	41.0	30.0	41.0
44	36.207077585944845	39.0	35.0	41.0	30.0	41.0
45	36.08629246007349	39.0	35.0	40.0	30.0	41.0
46	36.086627816330676	39.0	35.0	40.0	30.0	41.0
47	36.0643148583414	39.0	35.0	40.0	30.0	41.0
48	36.03898467461128	39.0	35.0	40.0	30.0	41.0
49	35.9851129277876	38.0	35.0	40.0	29.0	41.0
50	35.85635083363486	38.0	35.0	40.0	29.0	41.0
51	35.43315379026114	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	2.0
14	7.0
15	23.0
16	32.0
17	113.0
18	268.0
19	538.0
20	986.0
21	1719.0
22	2758.0
23	4071.0
24	5922.0
25	8546.0
26	10806.0
27	12287.0
28	13489.0
29	15517.0
30	18911.0
31	25013.0
32	33433.0
33	46633.0
34	74797.0
35	121571.0
36	102774.0
37	177727.0
38	250927.0
39	90912.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.44765745809763	22.17479513360809	29.998597779392455	13.378949628901825
2	29.951138004983274	25.97961779192419	30.887291860942863	13.181952342149671
3	29.11745411649806	25.467267954552362	32.152624358827275	13.262653570122307
4	26.876156464286026	27.326337919477233	30.535167790894587	15.262337825342145
5	24.106819793079307	31.866885138520768	29.676577326583065	14.349717741816868
6	21.223638497721637	40.585167251578966	27.435573846526463	10.75562040417293
7	87.23459542993751	4.573200328296125	6.337742973943211	1.8544612678231556
8	32.9911130591845	63.75436232792154	2.1987407470599947	1.0557838658339633
9	84.20246496654772	5.581720534491195	7.349499073847997	2.866315425113085
10	54.25299393711188	25.496685170095247	11.240121944164164	9.010198948628718
11	47.32582802107449	20.107549340024768	20.044596498763006	12.522026140137733
12	42.21282178756652	21.892880151322156	22.599187496506705	13.295110564604617
13	22.092721102243456	41.03250504260103	23.40168913651647	13.473084718639042
14	14.671247907700543	43.9981525988639	26.911261008167198	14.419338485268348
15	13.638507527375172	24.5786719303871	47.94270703100869	13.84011351122904
16	15.942267733923247	19.737382711110197	46.633248709809955	17.687100845156603
17	16.466776687052796	19.373197582689343	28.24984237275338	35.91018335750448
18	21.677546133548276	24.263025207612	33.370987369228224	20.688441289611507
19	28.444780454417533	25.449323453071205	25.35518836333399	20.75070772917727
20	30.590570213500346	24.579456389468245	25.18045010300928	19.649523294022128
21	23.858146264356826	27.97890981760346	27.76298745551872	20.399956462520997
22	23.194591939094597	23.83618141008481	26.412737262100528	26.556489388720067
23	19.808866544879393	30.30894940336984	25.832041427284075	24.05014262446669
24	20.443395884139314	24.922951409623938	37.03264624523563	17.601006461001106
25	17.708967642043476	26.046296813821385	34.96196844317232	21.282767100962825
26	16.865870244584535	35.64611481931456	27.056189823408456	20.43182511269245
27	18.36820744235942	33.81754070116914	29.441533774395452	18.37271808207599
28	14.888543073177285	28.791021081357233	39.33405307454028	16.9863827709252
29	16.149070759189694	25.9558879047196	37.382220823270195	20.51282051282051
30	19.255626777902965	29.55724148886411	32.4297345292412	18.757397203991722
31	29.56047738257383	25.80919405654577	25.0226757703143	19.607652790566096
32	30.43495314327851	27.57814928452429	25.552185650086145	16.43471192211106
33	26.497262728093734	27.416256541653304	25.822235688769783	20.264245041483175
34	19.61275177459353	28.332308633658588	28.182084719619617	23.872854872128265
35	20.202566946228274	26.292224735759863	31.029082839859544	22.476125478152326
36	30.11714915803026	24.478653397541308	27.131007608272512	18.273189836155915
37	20.886223035444804	31.37169534354895	28.985370818710525	18.75671080229572
38	20.85670776251678	33.163595999650916	23.79813514464935	22.181561093182953
39	19.903688036312612	31.008883018520102	27.775931030357587	21.311497914809703
40	23.265585485938082	26.1751442178992	26.271436570109557	24.287833726053158
41	17.452253407739278	24.502187169975613	28.15815871764474	29.88740070464037
42	22.4833817246529	26.663862225451577	24.845289960590737	26.00746608930478
43	22.942094172351542	26.8585061349603	25.77781569330003	24.421583999388123
44	21.290415577003973	30.550072513436312	27.687777441114086	20.471734468445625
45	18.513038200215533	36.08237212581547	23.120460555926538	22.28412911804246
46	22.91454004712638	31.899832419928792	26.010800040399644	19.174827492545187
47	22.11556847298176	27.78936489212217	26.79163099829282	23.303435636603254
48	23.200965669128887	23.883052840183133	30.809630411909655	22.10635107877832
49	20.988693002919167	23.153800066875135	32.06466688435406	23.792840045851634
50	19.189143870776054	32.31765493802283	27.288095539271495	21.205105651929625
51	18.512842085445243	33.38883381332423	24.95305502686282	23.145269074367704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1424.0
1	1737.5
2	2051.0
3	4752.5
4	7454.0
5	5529.0
6	3604.0
7	3917.5
8	4231.0
9	4474.0
10	4717.0
11	4651.0
12	4585.0
13	4420.5
14	4256.0
15	4125.5
16	3995.0
17	4017.0
18	4039.0
19	4119.5
20	4200.0
21	4318.0
22	4436.0
23	4718.5
24	5001.0
25	6320.0
26	8613.0
27	9587.0
28	11724.0
29	13861.0
30	15453.0
31	17045.0
32	19233.0
33	21421.0
34	23750.5
35	26080.0
36	30277.5
37	34475.0
38	48368.0
39	62261.0
40	75349.5
41	88438.0
42	96359.0
43	104280.0
44	101743.0
45	99206.0
46	96447.0
47	93688.0
48	88420.0
49	83152.0
50	76291.0
51	69430.0
52	62383.5
53	55337.0
54	50268.0
55	45199.0
56	41321.0
57	37443.0
58	34001.5
59	30560.0
60	27265.0
61	23970.0
62	20080.5
63	16191.0
64	13283.0
65	10375.0
66	8322.0
67	6269.0
68	5064.0
69	3859.0
70	3337.0
71	2815.0
72	2298.5
73	1782.0
74	1280.5
75	531.5
76	284.0
77	235.0
78	186.0
79	151.5
80	117.0
81	84.5
82	52.0
83	40.5
84	29.0
85	16.5
86	4.0
87	2.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1019811.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.05093919038595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.27413907179745	17.260182808520184
2	5.550907494231281	2.4480544721350324
3	1.9488249955997843	1.2892026441202502
4	1.111265790972198	0.9801781752433631
5	0.7538142404624868	0.8311155988642633
6	0.6558599692042126	0.8677396978998296
7	0.5138600389691805	0.7931767530175129
8	0.4736004197678955	0.8354667245474499
9	0.4276489170211949	0.8487054237661897
>10	8.700608476134528	48.525583290666276
>50	1.490659615534943	21.384521354672113
>100	0.09657542799026107	3.1381711141424544
>500	8.942169258357506E-4	0.15054939652200844
>1k	0.0013413253887536258	0.647352545883109
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2717	0.26642191543334987	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2459	0.2411231100664731	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1390	0.13629976534867735	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021180395190873603	0.0
2	0.0	0.0	0.0	0.06599262020119415	0.0
3	0.0	0.0	0.0	0.09619429482521762	0.0
4	0.0	0.0	0.0	0.15689181622869336	0.0
5	0.0	0.0	0.0	0.28034606412364643	0.0
6	0.0	0.0	0.0	0.39066062240944643	0.0
7	0.0	0.0	0.0	0.45949690677978566	0.0
8	0.0	0.0	0.0	0.6274692075296305	0.0
9	0.0	0.0	0.0	0.7949512213537606	0.0
10	0.0	0.0	0.0	1.1189328218660124	0.0
11	0.0	0.0	0.0	1.4460522587028382	0.0
12	0.0	0.0	0.0	1.7427739061453544	0.0
13	0.0	0.0	0.0	1.8529904070460115	0.0
14	0.0	0.0	0.0	1.894860910502044	0.0
15	0.0	0.0	0.0	1.9660505721158137	0.0
16	0.0	0.0	0.0	2.1006833619170613	0.0
17	0.0	0.0	0.0	2.2554179156726097	0.0
18	0.0	0.0	0.0	2.4986982882122275	0.0
19	0.0	0.0	0.0	2.607345870950598	0.0
20	0.0	0.0	0.0	2.7199157490946853	0.0
21	0.0	0.0	0.0	2.8695513188228015	0.0
22	0.0	0.0	0.0	3.012518986361198	0.0
23	0.0	0.0	0.0	3.180883516651615	0.0
24	0.0	0.0	0.0	3.298356264052849	0.0
25	0.0	0.0	0.0	3.3949427884186383	0.0
26	0.0	0.0	0.0	3.4950593786495734	0.0
27	0.0	0.0	0.0	3.585076058210786	0.0
28	0.0	0.0	0.0	3.688428542151438	0.0
29	0.0	0.0	0.0	3.7949188624166634	0.0
30	0.0	0.0	0.0	3.943083571367636	0.0
31	0.0	0.0	0.0	4.089287132615749	0.0
32	0.0	0.0	0.0	4.209701601571272	0.0
33	0.0	0.0	0.0	4.327762693283363	0.0
34	0.0	0.0	0.0	4.4523936298000315	0.0
35	0.0	0.0	0.0	4.614580544826444	0.0
36	0.0	0.0	0.0	4.74950750678312	0.0
37	0.0	0.0	0.0	4.883257780118081	0.0
38	0.0	0.0	0.0	5.012007126810752	0.0
39	0.0	0.0	0.0	5.149581638166287	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGA	75	0.0	45.0	2
CAACGGC	20	7.0329127E-4	45.0	15
GCGCGTA	20	7.0329127E-4	45.0	24
CTATACG	20	7.0329127E-4	45.0	1
CCTACGG	20	7.0329127E-4	45.0	22
ATTCGAA	20	7.0329127E-4	45.0	15
GCACCGT	20	7.0329127E-4	45.0	36
TACGCGT	20	7.0329127E-4	45.0	13
TACGATT	25	3.890496E-5	45.0	16
CGTGTAA	20	7.0329127E-4	45.0	34
TACTAGC	35	1.2117562E-7	45.0	42
ATACGTG	20	7.0329127E-4	45.0	1
CACGTAG	25	3.890496E-5	45.0	1
ATACGAT	20	7.0329127E-4	45.0	10
CGACTAA	25	3.890496E-5	45.0	33
TATCGTG	20	7.0329127E-4	45.0	1
AGCGTAT	30	2.1652613E-6	44.999996	31
TATGCGC	30	2.1652613E-6	44.999996	16
AAATTCG	30	2.1652613E-6	44.999996	11
CTACCTA	30	2.1652613E-6	44.999996	2
>>END_MODULE