##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549234_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	909746
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.776341967977874	31.0	31.0	34.0	30.0	34.0
2	32.117574575760706	33.0	31.0	34.0	30.0	34.0
3	32.14557250045617	33.0	31.0	34.0	30.0	34.0
4	35.872610596803945	37.0	35.0	37.0	35.0	37.0
5	35.84842252672724	37.0	35.0	37.0	35.0	37.0
6	35.89980500051663	37.0	35.0	37.0	35.0	37.0
7	36.13362630888182	37.0	35.0	37.0	35.0	37.0
8	13.231430531159246	0.0	0.0	36.0	0.0	37.0
9	24.941885976965	18.0	17.0	39.0	17.0	39.0
10	32.76725481617946	32.0	32.0	38.0	27.0	39.0
11	35.62920639387258	37.0	35.0	37.0	32.0	39.0
12	36.58013665352747	37.0	35.0	39.0	33.0	39.0
13	36.71679128020349	39.0	35.0	39.0	33.0	39.0
14	37.894806902146314	40.0	37.0	41.0	33.0	41.0
15	38.22118041739123	40.0	37.0	41.0	33.0	41.0
16	38.34166459649177	40.0	37.0	41.0	34.0	41.0
17	38.305595188107446	40.0	37.0	41.0	34.0	41.0
18	38.27687728223042	40.0	37.0	41.0	34.0	41.0
19	38.25814348180701	40.0	37.0	41.0	34.0	41.0
20	38.22911340088332	40.0	37.0	41.0	34.0	41.0
21	38.14370384700785	40.0	36.0	41.0	34.0	41.0
22	38.07360735853744	40.0	36.0	41.0	33.0	41.0
23	38.001078322960474	40.0	36.0	41.0	34.0	41.0
24	37.91821453460636	40.0	36.0	41.0	34.0	41.0
25	37.81752599077105	40.0	35.0	41.0	33.0	41.0
26	37.73628683170907	40.0	35.0	41.0	33.0	41.0
27	37.674242041185124	40.0	35.0	41.0	33.0	41.0
28	37.617794417342864	40.0	35.0	41.0	33.0	41.0
29	37.65013751090964	40.0	36.0	41.0	33.0	41.0
30	37.57930565234692	40.0	36.0	41.0	33.0	41.0
31	37.513149824236656	40.0	35.0	41.0	33.0	41.0
32	37.36987906514565	40.0	35.0	41.0	33.0	41.0
33	37.254461135305895	40.0	35.0	41.0	32.0	41.0
34	37.10778063327566	40.0	35.0	41.0	32.0	41.0
35	37.0197417740776	40.0	35.0	41.0	32.0	41.0
36	36.91408261206974	40.0	35.0	41.0	31.0	41.0
37	36.83114737520143	40.0	35.0	41.0	31.0	41.0
38	36.767967102905644	40.0	35.0	41.0	31.0	41.0
39	36.70931996403392	39.0	35.0	41.0	31.0	41.0
40	36.593827288056225	39.0	35.0	41.0	31.0	41.0
41	36.56136877765882	39.0	35.0	41.0	31.0	41.0
42	36.51143505989584	39.0	35.0	41.0	31.0	41.0
43	36.41060361903212	39.0	35.0	41.0	30.0	41.0
44	36.19593820692809	39.0	35.0	41.0	30.0	41.0
45	36.125569114895804	39.0	35.0	40.0	30.0	41.0
46	36.10923048850998	39.0	35.0	40.0	30.0	41.0
47	36.08759587841002	39.0	35.0	40.0	30.0	41.0
48	36.02027379070642	38.0	35.0	40.0	30.0	41.0
49	35.94102529717086	38.0	35.0	40.0	29.0	41.0
50	35.83753597157888	38.0	35.0	40.0	29.0	41.0
51	35.44126162687168	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	3.0
13	3.0
14	4.0
15	9.0
16	36.0
17	90.0
18	215.0
19	441.0
20	802.0
21	1440.0
22	2287.0
23	3389.0
24	5080.0
25	7615.0
26	9633.0
27	10959.0
28	11935.0
29	13637.0
30	16861.0
31	22269.0
32	29798.0
33	41143.0
34	65419.0
35	103735.0
36	94697.0
37	163212.0
38	221435.0
39	83572.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.57184752667228	23.08820264117677	29.124173120849118	13.215776711301835
2	31.833940462502717	24.93201399071829	30.316923624835944	12.917121921943048
3	30.7847465116637	24.195874452869262	31.77963959171021	13.239739443756829
4	27.15285365365718	26.808801577583193	31.397005317967874	14.64133945079176
5	23.97361461330965	32.27505259709853	28.963359003502077	14.787973786089745
6	21.78827936588894	41.08641313069802	26.224902335377127	10.900405168035913
7	87.23237035392296	4.237666337637099	6.469388158892701	2.0605751495472364
8	33.030318352595124	63.719104013647765	2.3505461964108663	0.9000314373462484
9	84.18723467869054	5.366992545171949	7.5112174167295045	2.9345553594080105
10	51.731802063433086	25.493489391544454	12.384555689170384	10.390152855852074
11	45.10390812380599	21.083027570332817	19.882692531761613	13.930371774099584
12	40.32938864254418	21.07071644173209	24.03187263258096	14.568022283142767
13	23.62098871553159	35.949155038878985	24.700410883917048	15.72944536167238
14	17.16105374467159	38.30926434411363	28.668111758666704	15.861570152548074
15	16.250030228217547	23.78872784271654	44.59068795026304	15.370553978802876
16	19.6464727517351	19.604592930334398	42.55165727576708	18.197277042163417
17	19.145783548375043	19.771452691190728	28.560609224992472	32.52215453544176
18	23.651107012286946	24.493759796690505	31.411404941599088	20.443728249423465
19	28.775284529967703	25.8427077447991	25.339270521662087	20.042737203571107
20	32.14798416261242	23.64572089352413	24.755261358664946	19.45103358519851
21	25.585053410512387	26.99456771450493	27.249034345850383	20.171344529132305
22	23.51062824128933	23.135138818967054	27.66860200539491	25.68563093434871
23	22.177618807887036	28.636014887671944	25.8940407542325	23.29232555020852
24	22.888476563788135	23.853141426288214	34.95690005781834	18.301481952105313
25	19.956449382574917	24.29469324404834	32.96920239275578	22.779654980620965
26	18.80436957128693	32.619544356336824	27.379950008024217	21.19613606435203
27	19.969749798295346	30.78936318488897	29.63915202704931	19.601734989766374
28	17.44300057378653	27.823480400023744	37.056607008989324	17.676912017200404
29	18.98244125283321	25.492719946006908	34.80114229686088	20.723696504299003
30	21.43147647804992	27.775994618278073	31.7009363052984	19.091592598373612
31	30.588867661962787	24.401096569811795	25.039186762019288	19.97084900620613
32	29.90757859886166	26.220395582942928	26.044962000382526	17.827063817812885
33	28.06002994242349	26.64392039096627	25.410389273489525	19.885660393120716
34	21.095668461306783	27.61968725336523	28.20215752528728	23.082486760040716
35	21.83004926649856	26.163016930000243	30.458941286908654	21.547992516592544
36	29.635524640943732	25.511406480490155	26.77406660760256	18.079002270963542
37	22.500785933656207	31.33380086309805	27.026774506290767	19.138638696954974
38	21.901058097534918	32.44784808067307	23.33266648053413	22.31842734125789
39	22.78471133701055	29.449648583230925	26.079147366407767	21.68649271335076
40	24.298540471736064	25.92789635788451	25.612313766699717	24.161249403679708
41	19.132702974236764	25.444904401888003	27.8825078648326	27.539884759042632
42	23.35234230213708	26.82990636947016	23.544923528105645	26.272827800287114
43	24.21148320520233	26.045841366711148	24.306784531066914	25.435890897019608
44	20.661921019713194	30.154680537204893	27.489431115937858	21.69396732714406
45	20.666098009774156	33.20882971730571	24.28029362041713	21.844778652503006
46	23.318047015320758	31.25256939849145	25.76906081477687	19.66032277141092
47	23.614833151231224	27.0335895953376	25.965379347642088	23.38619790578909
48	24.45220973766304	23.698153110868308	30.07333915180721	21.776297999661445
49	21.95887643364192	24.22434393775845	30.67317690871958	23.143602719880054
50	19.687473206807173	30.713517839045185	26.92158030922917	22.67742864491847
51	19.84433017567541	31.51868763369116	26.257109127162963	22.379873063470463
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	592.0
1	1165.5
2	1739.0
3	5096.5
4	8454.0
5	6031.0
6	3608.0
7	3650.0
8	3692.0
9	3821.0
10	3950.0
11	3869.0
12	3788.0
13	3621.0
14	3454.0
15	3254.0
16	3054.0
17	2984.5
18	2915.0
19	2925.5
20	2936.0
21	3281.5
22	3627.0
23	3869.0
24	4111.0
25	5075.0
26	7926.0
27	9813.0
28	11082.5
29	12352.0
30	13885.5
31	15419.0
32	16686.5
33	17954.0
34	20189.0
35	22424.0
36	26114.5
37	29805.0
38	38233.0
39	46661.0
40	58589.5
41	70518.0
42	74719.0
43	78920.0
44	77619.0
45	76318.0
46	74721.0
47	73124.0
48	69708.5
49	66293.0
50	63311.0
51	60329.0
52	56510.5
53	52692.0
54	50132.0
55	47572.0
56	46195.0
57	44818.0
58	42249.0
59	39680.0
60	35345.0
61	31010.0
62	26804.5
63	22599.0
64	18668.5
65	14738.0
66	11929.5
67	9121.0
68	7577.0
69	6033.0
70	4980.0
71	3927.0
72	3228.0
73	2529.0
74	2046.5
75	1201.5
76	839.0
77	628.5
78	418.0
79	306.0
80	194.0
81	132.5
82	71.0
83	51.0
84	31.0
85	23.0
86	15.0
87	8.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	909746.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.050183446517384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.56957668476848	16.957922386665413
2	5.169933184873334	2.383358166351364
3	2.29085097010932	1.5841360532895636
4	1.6110195392274387	1.4853718366046547
5	1.3128150062315127	1.5130313362488614
6	1.1009075361941407	1.5225672400157053
7	0.9666213187863074	1.5596559104937262
8	0.9169151173747894	1.6908049328299128
9	0.804901411662098	1.6697812675654948
>10	11.601733836877129	58.67481185948835
>50	0.6081991204884303	8.614671411804265
>100	0.0441280118912018	1.6359672074111309
>500	0.001438956909495711	0.22443829364361664
>1k	9.59304606330474E-4	0.4834820975879486
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2824	0.3104163140041286	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1549	0.17026730537974336	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	954	0.10486443468836355	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0992079107794923E-4	0.0	0.0	0.009233346450547734	0.0
2	1.0992079107794923E-4	0.0	0.0	0.030338138337513986	0.0
3	1.0992079107794923E-4	0.0	0.0	0.0428691085204002	0.0
4	1.0992079107794923E-4	0.0	0.0	0.06628223702000338	0.0
5	1.0992079107794923E-4	0.0	0.0	0.1221219988876016	0.0
6	1.0992079107794923E-4	0.0	0.0	0.1777419191730439	0.0
7	1.0992079107794923E-4	0.0	0.0	0.2085197406748697	0.0
8	1.0992079107794923E-4	0.0	0.0	0.28216667069709567	0.0
9	1.0992079107794923E-4	0.0	0.0	0.36471718479663556	0.0
10	1.0992079107794923E-4	0.0	0.0	0.5311372624886507	0.0
11	1.0992079107794923E-4	0.0	0.0	0.7130561717226567	0.0
12	1.0992079107794923E-4	0.0	0.0	0.8757389425180215	0.0
13	1.0992079107794923E-4	0.0	0.0	0.9397128429253879	0.0
14	1.0992079107794923E-4	0.0	0.0	0.9653243872465501	0.0
15	1.0992079107794923E-4	0.0	0.0	1.006654604691859	0.0
16	1.0992079107794923E-4	0.0	0.0	1.0882158316716974	0.0
17	1.0992079107794923E-4	0.0	0.0	1.1873643852240077	0.0
18	1.0992079107794923E-4	0.0	0.0	1.3374062650454084	0.0
19	1.0992079107794923E-4	0.0	0.0	1.4054472347226588	0.0
20	1.0992079107794923E-4	0.0	0.0	1.4874481448668089	0.0
21	1.0992079107794923E-4	0.0	0.0	1.5880256687031324	0.0
22	1.0992079107794923E-4	0.0	0.0	1.6877238262108325	0.0
23	1.0992079107794923E-4	0.0	0.0	1.8070978053214852	0.0
24	1.0992079107794923E-4	0.0	0.0	1.8872300620173104	0.0
25	1.0992079107794923E-4	0.0	0.0	1.9583488138447436	0.0
26	1.0992079107794923E-4	0.0	0.0	2.0299072488364884	0.0
27	1.0992079107794923E-4	0.0	0.0	2.101905366992545	0.0
28	1.0992079107794923E-4	0.0	0.0	2.185445168211787	0.0
29	1.0992079107794923E-4	0.0	0.0	2.2675559991470147	0.0
30	1.0992079107794923E-4	0.0	0.0	2.3762676615231064	0.0
31	1.0992079107794923E-4	0.0	0.0	2.475086452702183	0.0
32	1.0992079107794923E-4	0.0	0.0	2.5681893627452057	0.0
33	1.0992079107794923E-4	0.0	0.0	2.669646252910153	0.0
34	1.0992079107794923E-4	0.0	0.0	2.766486469849826	0.0
35	1.0992079107794923E-4	0.0	0.0	2.9023485676221714	0.0
36	1.0992079107794923E-4	0.0	0.0	3.0064435567729895	0.0
37	1.0992079107794923E-4	0.0	0.0	3.119552050792199	0.0
38	1.0992079107794923E-4	0.0	0.0	3.2361780101259034	0.0
39	1.0992079107794923E-4	0.0	0.0	3.353353573414997	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGA	30	2.1650012E-6	45.000004	2
TAGCGTG	30	2.1650012E-6	45.000004	1
TCTAGCG	30	2.1650012E-6	45.000004	1
GACGATT	30	2.1650012E-6	45.000004	7
CACGTCA	35	1.2115561E-7	45.000004	34
GTCGCCC	20	7.0325093E-4	45.0	16
CGAACTG	20	7.0325093E-4	45.0	12
ACGCATG	20	7.0325093E-4	45.0	1
AAACGCC	20	7.0325093E-4	45.0	44
CTAACGT	20	7.0325093E-4	45.0	31
TATTCGG	25	3.8901628E-5	45.0	34
TCGTATA	20	7.0325093E-4	45.0	15
ACGCTAA	25	3.8901628E-5	45.0	39
CTACCGA	20	7.0325093E-4	45.0	2
CGGTCTA	80	0.0	45.0	31
CCGGTAC	20	7.0325093E-4	45.0	20
AATAGAC	25	3.8901628E-5	45.0	27
ACGACTA	20	7.0325093E-4	45.0	21
TACGTAG	65	0.0	44.999996	1
CGTAAGA	100	0.0	42.75	2
>>END_MODULE