##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549231_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1043731
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.771879919251226	31.0	31.0	34.0	30.0	34.0
2	32.101941975470695	33.0	31.0	34.0	30.0	34.0
3	32.12295601069624	33.0	31.0	34.0	30.0	34.0
4	35.84945354693882	37.0	35.0	37.0	35.0	37.0
5	35.82770464803671	37.0	35.0	37.0	35.0	37.0
6	35.87256965635782	37.0	35.0	37.0	35.0	37.0
7	36.10487951397439	37.0	35.0	37.0	35.0	37.0
8	13.16018111946469	0.0	0.0	36.0	0.0	37.0
9	24.8996935034027	18.0	17.0	39.0	17.0	39.0
10	32.79638623361767	32.0	32.0	38.0	27.0	39.0
11	35.654017174923425	37.0	35.0	37.0	32.0	39.0
12	36.47702425241753	37.0	35.0	39.0	33.0	39.0
13	36.4778338479934	39.0	35.0	39.0	33.0	39.0
14	37.63264576792296	39.0	36.0	41.0	33.0	41.0
15	38.02725319071676	40.0	36.0	41.0	33.0	41.0
16	38.19522942214038	40.0	36.0	41.0	34.0	41.0
17	38.190803952359374	40.0	36.0	41.0	34.0	41.0
18	38.14190821198182	40.0	36.0	41.0	34.0	41.0
19	38.08917144359993	40.0	36.0	41.0	34.0	41.0
20	38.075274184631866	40.0	36.0	41.0	34.0	41.0
21	37.954508393446204	40.0	36.0	41.0	33.0	41.0
22	37.88502305670714	40.0	35.0	41.0	33.0	41.0
23	37.824941483964736	40.0	35.0	41.0	33.0	41.0
24	37.72795672448169	40.0	35.0	41.0	33.0	41.0
25	37.63298493577368	40.0	35.0	41.0	33.0	41.0
26	37.51776846716252	40.0	35.0	41.0	33.0	41.0
27	37.4472617944662	39.0	35.0	41.0	33.0	41.0
28	37.42680729038421	39.0	35.0	41.0	33.0	41.0
29	37.47094797414276	40.0	35.0	41.0	33.0	41.0
30	37.39078076630856	40.0	35.0	41.0	33.0	41.0
31	37.28321090395897	40.0	35.0	41.0	33.0	41.0
32	37.090614344117405	39.0	35.0	41.0	32.0	41.0
33	36.95278476925568	40.0	35.0	41.0	32.0	41.0
34	36.82876430804489	40.0	35.0	41.0	31.0	41.0
35	36.70083287743681	39.0	35.0	41.0	31.0	41.0
36	36.57674726533944	39.0	35.0	41.0	31.0	41.0
37	36.460629223430175	39.0	35.0	41.0	31.0	41.0
38	36.39973709701063	39.0	35.0	41.0	30.0	41.0
39	36.36370386622607	39.0	35.0	41.0	30.0	41.0
40	36.19781150507171	39.0	35.0	41.0	30.0	41.0
41	36.17515528426386	39.0	35.0	41.0	30.0	41.0
42	36.12476873830518	39.0	35.0	41.0	30.0	41.0
43	36.01423834302133	39.0	35.0	41.0	30.0	41.0
44	35.76371018969447	38.0	35.0	40.0	29.0	41.0
45	35.69805150944065	38.0	35.0	40.0	29.0	41.0
46	35.71486331248185	38.0	35.0	40.0	29.0	41.0
47	35.68218439425484	38.0	35.0	40.0	29.0	41.0
48	35.641532157232085	38.0	35.0	40.0	29.0	41.0
49	35.56603090259846	38.0	35.0	40.0	28.0	41.0
50	35.43083323193428	38.0	35.0	40.0	28.0	41.0
51	35.028439320093014	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	3.0
14	3.0
15	24.0
16	55.0
17	118.0
18	282.0
19	589.0
20	1107.0
21	1969.0
22	3120.0
23	4616.0
24	6918.0
25	9874.0
26	12658.0
27	14077.0
28	15057.0
29	16943.0
30	20867.0
31	26655.0
32	36005.0
33	50339.0
34	81440.0
35	131894.0
36	104644.0
37	176471.0
38	246425.0
39	81554.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.74413522258129	23.62936427106218	28.212633331768433	14.413867174588088
2	30.146943992273872	27.311634894431613	30.418182462722676	12.123238650571842
3	30.32275557590988	25.338042081724122	32.17169941297135	12.167502929394642
4	27.066456778614413	27.05486375320844	31.74208680205915	14.136592666117995
5	24.02812602097667	32.634653948191634	29.533854987539893	13.803365043291807
6	21.50688252049618	41.15428208992547	26.881734853137445	10.457100536440903
7	85.57137806580431	5.635264258702674	7.005636509790358	1.7877211657026573
8	32.28609670499391	64.22344454653546	2.44804456320642	1.0424141852642108
9	83.22652100972377	5.982001109481275	7.747973376281819	3.043504504513136
10	56.37487053656546	22.06967120838607	11.636044153139075	9.9194141019094
11	51.75567267811342	18.70194523301502	17.574643274943448	11.96773881392811
12	44.94405167615027	20.885170604303216	21.45112102639473	12.719656693151778
13	21.31459159496077	42.864301242369926	21.83560706733823	13.985500095331076
14	14.614206150818553	45.568829516417544	26.727767978530864	13.089196354233035
15	13.749519751736797	23.095701861878204	50.14874522266752	13.00603316371747
16	16.02146530092524	18.188594570823323	47.79191190067172	17.998028227579713
17	16.3710764555235	18.82678582891569	27.787044746203765	37.01509296935705
18	22.714090124754367	23.92762119741581	33.62513904444728	19.73314963338255
19	30.413104525974603	25.181775764061815	24.58919012657476	19.815929583388822
20	33.57301833518407	24.4426964418993	22.83308630288839	19.151198920028246
21	23.19726059683961	28.1556263060118	27.48581770590315	21.161295391245446
22	22.859338277774636	24.6132384685326	26.06955240382819	26.457870849864573
23	20.631082146645063	30.13860851119685	23.917273703665025	25.31303563849306
24	20.870799085204904	24.49012245492373	37.81846088695267	16.820617572918692
25	18.286320900691845	24.774295292561014	35.30315761436616	21.636226192380985
26	16.140653099313905	37.124316514504216	26.801062725932255	19.933967660249625
27	18.68249577716864	33.585856892245225	30.115614080639553	17.616033249946586
28	14.855360241288224	29.607053924813957	39.38361512688614	16.153970707011673
29	15.36363296673185	26.672581345193347	37.5109103782488	20.452875309826
30	18.69926254945	30.877112972595427	32.02779260173359	18.39583187622098
31	31.968581943048548	26.130391834677706	23.155199950945217	18.745826271328532
32	31.2740543300908	29.107691541211288	24.29083739009381	15.327416738604105
33	28.368708029176098	29.42587697404791	23.625052815332687	18.580362181443302
34	20.411581144950183	29.83211191389352	27.033306474560977	22.72300046659532
35	20.575033222161647	27.472308477950737	29.173992149318167	22.77866615056945
36	31.67655267497085	24.62780160788556	26.072714138029816	17.622931579113775
37	20.99362766843181	33.00591819156469	28.312371674310715	17.68808246569279
38	20.85805633827107	34.92030034558713	21.603075888327545	22.618567427814256
39	22.26014174150236	30.654066996189634	26.595645812953723	20.490145449354287
40	24.192727819716	26.977161739950233	24.790966254715055	24.039144185618706
41	18.856582778512852	24.205470566649836	27.584023086408276	29.353923568429032
42	23.63166371411791	25.917022681131442	23.327179129488346	27.124134475262302
43	24.211410794543806	26.021934770549116	24.971951585226464	24.79470284968062
44	19.810085165622176	31.435206964246536	27.610179251167207	21.14452861896408
45	17.94945249302742	37.55574951783554	22.29530405822956	22.199493930907487
46	23.791475006491137	32.826082582581144	24.605669468474154	18.77677294245356
47	23.31635258510095	28.484159232599204	25.576896729138067	22.62259145316178
48	24.878440900960115	24.240537073249715	29.38237917624369	21.49864284954648
49	21.545877242316266	23.691257613312242	31.576431091919275	23.186434052452213
50	19.83509160885324	32.82914850665545	27.491278883160508	19.844481001330802
51	19.516618745634652	34.642355166225784	22.960226341844788	22.880799746294784
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1840.0
1	2187.0
2	2534.0
3	6267.0
4	10000.0
5	7468.5
6	4937.0
7	5045.0
8	5153.0
9	5477.5
10	5802.0
11	5827.5
12	5853.0
13	5475.0
14	5097.0
15	4749.0
16	4401.0
17	4341.0
18	4281.0
19	4379.0
20	4477.0
21	4537.0
22	4597.0
23	4901.5
24	5206.0
25	6679.5
26	10422.0
27	12691.0
28	13707.0
29	14723.0
30	15172.0
31	15621.0
32	16270.5
33	16920.0
34	17641.5
35	18363.0
36	24698.0
37	31033.0
38	45447.0
39	59861.0
40	77181.0
41	94501.0
42	100732.0
43	106963.0
44	102271.0
45	97579.0
46	93608.0
47	89637.0
48	84484.0
49	79331.0
50	71784.0
51	64237.0
52	58763.5
53	53290.0
54	50117.0
55	46944.0
56	44908.5
57	42873.0
58	39751.0
59	36629.0
60	32991.5
61	29354.0
62	25631.5
63	21909.0
64	17911.0
65	13913.0
66	11382.5
67	8852.0
68	7302.0
69	5752.0
70	4981.0
71	4210.0
72	3500.0
73	2790.0
74	2240.5
75	1382.5
76	1074.0
77	724.5
78	375.0
79	239.5
80	104.0
81	105.5
82	107.0
83	81.5
84	56.0
85	31.0
86	6.0
87	5.5
88	5.0
89	4.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1043731.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.551505034485036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70437946315707	16.961978302362503
2	5.466467250292604	2.3562119293105726
3	1.9510943467969222	1.2614705891324747
4	1.1215406798967853	0.9668355843670136
5	0.772795486066852	0.8327452904298536
6	0.6429858938305494	0.8314388236795169
7	0.5298295707875793	0.7993037263573293
8	0.4658706532381011	0.8032170982943815
9	0.43532499223499627	0.8443717885610715
>10	8.09079924887787	43.964942678109665
>50	1.6408245572444344	23.51690129309089
>100	0.17316583136181288	5.390451101832092
>500	0.0017898277143339834	0.25362063755086767
>1k	0.0031321985000844705	1.2165111569217475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3511	0.3363893570278166	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2275	0.2179680396577279	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2013	0.19286578629934342	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1327	0.12714003895639778	No Hit
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1214	0.11631349456900292	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1164	0.111522988202899	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1111	0.10644505145482887	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02337767106658708	0.0
2	0.0	0.0	0.0	0.06476764606972486	0.0
3	0.0	0.0	0.0	0.09264839312044962	0.0
4	0.0	0.0	0.0	0.144577482129016	0.0
5	0.0	0.0	0.0	0.24671107785435137	0.0
6	0.0	0.0	0.0	0.34022176212069966	0.0
7	0.0	0.0	0.0	0.3956000157128609	0.0
8	0.0	0.0	0.0	0.49945819372999367	0.0
9	0.0	0.0	0.0	0.5987174856356666	0.0
10	0.0	0.0	0.0	0.8272246392988232	0.0
11	0.0	0.0	0.0	1.1046907680235616	0.0
12	0.0	0.0	0.0	1.3451741876019778	0.0
13	0.0	0.0	0.0	1.4476910238366016	0.0
14	0.0	0.0	0.0	1.49233854316869	0.0
15	0.0	0.0	0.0	1.563621277896316	0.0
16	0.0	0.0	0.0	1.692006848507901	0.0
17	0.0	0.0	0.0	1.846644394005735	0.0
18	0.0	0.0	0.0	2.074576686904959	0.0
19	0.0	0.0	0.0	2.178722295304058	0.0
20	0.0	0.0	0.0	2.276448625172578	0.0
21	0.0	0.0	0.0	2.4135529173704717	0.0
22	0.0	0.0	0.0	2.546058323456906	0.0
23	0.0	0.0	0.0	2.6964802233525687	0.0
24	0.0	0.0	0.0	2.8138476293221144	0.0
25	0.0	0.0	0.0	2.8985437818748316	0.0
26	0.0	0.0	0.0	2.992916757287079	0.0
27	0.0	0.0	0.0	3.0724391629644034	0.0
28	0.0	0.0	0.0	3.1667163282493287	0.0
29	0.0	0.0	0.0	3.269233164483952	0.0
30	0.0	0.0	0.0	3.386504760326176	0.0
31	0.0	0.0	0.0	3.512782508136675	0.0
32	0.0	0.0	0.0	3.615778395007909	0.0
33	0.0	0.0	0.0	3.7181036109878884	0.0
34	0.0	0.0	0.0	3.8315428017372293	0.0
35	0.0	0.0	0.0	3.9827311826514684	0.0
36	0.0	0.0	0.0	4.106901107660882	0.0
37	0.0	0.0	0.0	4.2318375136888715	0.0
38	0.0	0.0	0.0	4.342689831000516	0.0
39	0.0	0.0	0.0	4.463314781299013	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGC	20	7.032987E-4	45.000004	6
GTCGAAA	25	3.890558E-5	45.000004	40
GGTCGAA	25	3.890558E-5	45.000004	39
ACGTTAG	25	3.890558E-5	45.000004	1
ACGTTAC	35	1.2117926E-7	45.000004	27
CGTTGGC	25	3.890558E-5	45.000004	24
ATTACGC	25	3.890558E-5	45.000004	19
CAATTCG	30	2.1653086E-6	45.000004	44
CGATTAC	40	6.8139343E-9	45.000004	10
CCGCTCG	50	2.1827873E-11	45.000004	19
CGTTCCG	20	7.032987E-4	45.000004	37
TCGGCGC	35	1.2117926E-7	45.000004	18
TTATACG	50	2.1827873E-11	45.000004	1
GTTACGA	30	2.1653086E-6	45.000004	2
CCGTACA	25	3.890558E-5	45.000004	30
ACTCGTT	30	2.1653086E-6	45.000004	39
CGTACGT	35	1.2117926E-7	45.000004	12
GTATCGA	30	2.1653086E-6	45.000004	2
GCGATAC	125	0.0	45.000004	9
CGTAAGA	60	0.0	45.000004	2
>>END_MODULE