##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549229_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2113138
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00936001340187	33.0	31.0	34.0	30.0	34.0
2	32.386247845621064	34.0	31.0	34.0	31.0	34.0
3	32.4387583773516	34.0	31.0	34.0	31.0	34.0
4	36.01842615106065	37.0	35.0	37.0	35.0	37.0
5	35.96181508259281	37.0	35.0	37.0	35.0	37.0
6	36.02596990825966	37.0	35.0	37.0	35.0	37.0
7	36.29479617516698	37.0	36.0	37.0	35.0	37.0
8	36.18978741568227	37.0	37.0	37.0	35.0	37.0
9	38.042017132813854	39.0	39.0	39.0	35.0	39.0
10	37.721030051042575	39.0	38.0	39.0	35.0	39.0
11	37.421704592885085	39.0	37.0	39.0	35.0	39.0
12	36.61779117123444	39.0	35.0	39.0	33.0	39.0
13	36.36414185916869	39.0	35.0	39.0	33.0	39.0
14	37.43323057935639	40.0	35.0	41.0	33.0	41.0
15	37.63652492170412	40.0	35.0	41.0	33.0	41.0
16	37.764747971973435	40.0	35.0	41.0	34.0	41.0
17	37.723178041377324	40.0	35.0	41.0	34.0	41.0
18	37.68321756553524	39.0	35.0	41.0	34.0	41.0
19	37.6542483264226	39.0	35.0	41.0	34.0	41.0
20	37.526919207358915	39.0	35.0	41.0	34.0	41.0
21	37.380407242688364	39.0	35.0	41.0	33.0	41.0
22	37.331083914065246	39.0	35.0	41.0	33.0	41.0
23	37.26904158649364	39.0	35.0	41.0	33.0	41.0
24	37.25063341816767	39.0	35.0	41.0	33.0	41.0
25	37.15317504110001	39.0	35.0	41.0	33.0	41.0
26	37.11365608871735	39.0	35.0	41.0	33.0	41.0
27	37.058476540576144	39.0	35.0	41.0	33.0	41.0
28	37.06054786767358	38.0	35.0	41.0	33.0	41.0
29	37.0096889081546	38.0	35.0	41.0	33.0	41.0
30	36.9895269499673	38.0	35.0	41.0	33.0	41.0
31	36.82935425892677	38.0	35.0	41.0	33.0	41.0
32	36.67110382757775	38.0	35.0	41.0	32.0	41.0
33	36.594080935556505	38.0	35.0	41.0	32.0	41.0
34	36.485376250864824	38.0	35.0	41.0	32.0	41.0
35	36.39725659185534	38.0	35.0	41.0	31.0	41.0
36	36.28422516655325	38.0	35.0	41.0	31.0	41.0
37	36.02993746740629	38.0	35.0	41.0	30.0	41.0
38	36.14619679358376	38.0	35.0	41.0	31.0	41.0
39	36.101940810302025	38.0	35.0	41.0	31.0	41.0
40	35.98698996468759	38.0	35.0	41.0	30.0	41.0
41	35.96167642624381	38.0	35.0	41.0	31.0	41.0
42	35.96664533977431	38.0	35.0	41.0	31.0	41.0
43	35.853567064716074	38.0	35.0	40.0	30.0	41.0
44	35.736759738360675	37.0	35.0	40.0	30.0	41.0
45	35.68977369201633	37.0	35.0	40.0	30.0	41.0
46	35.531257305485966	37.0	35.0	40.0	30.0	41.0
47	35.5164925338525	37.0	35.0	40.0	30.0	41.0
48	35.39276800663279	36.0	35.0	40.0	29.0	41.0
49	35.3854206398257	36.0	35.0	40.0	29.0	41.0
50	35.25197029252231	36.0	35.0	40.0	29.0	41.0
51	34.708538202426915	35.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	5.0
14	11.0
15	32.0
16	94.0
17	219.0
18	521.0
19	1065.0
20	1980.0
21	3192.0
22	4913.0
23	7283.0
24	10605.0
25	15944.0
26	22112.0
27	26590.0
28	28462.0
29	31077.0
30	35716.0
31	43771.0
32	55977.0
33	76920.0
34	157566.0
35	379496.0
36	116438.0
37	148652.0
38	257562.0
39	686592.0
40	337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.637054465917515	23.23317265602152	30.806317429339686	13.323455448721285
2	29.720254900531817	25.676837007332225	31.738674899604284	12.864233192531676
3	29.172065430653372	25.033149751696293	32.72270907058602	13.072075747064318
4	26.57237719448517	27.621054564349322	30.854492229092468	14.95207601207304
5	22.317946106690616	32.9671796162863	29.962785203805904	14.752089073217178
6	20.924000230936173	40.72559387981286	27.494512899772754	10.855892989478207
7	87.76454732251278	4.227078401883833	6.209722223536749	1.7986520520666422
8	89.1985757674132	3.227995521352604	5.594476082489643	1.9789526287445496
9	85.01058615196925	5.2927447237236755	7.1409439421372385	2.5557251821698346
10	50.907134318724104	27.65668877281086	11.967746545658636	9.468430362806405
11	44.27519641405341	20.179089108236187	22.409042854749668	13.136671622960735
12	39.679661243136984	21.22492709894006	24.26391461418989	14.831497043733064
13	22.938918329044295	38.137499775215815	23.966158386248317	14.957423509491571
14	15.74819060563011	41.008679982092985	27.959366591296924	15.283762820979982
15	14.262627428970564	24.474643870868825	46.45640748498205	14.806321215178563
16	15.94453367456361	19.99476607774788	44.907431507076204	19.153268740612305
17	16.53895770176865	20.385653942146703	29.45406310425538	33.62132525182927
18	21.726030197743828	24.22690803913422	33.195371054800965	20.85169070832099
19	28.087848498299685	25.076024377016548	26.07449205872972	20.761635065954046
20	30.32177737563756	24.159330815119503	25.60571055936716	19.913181249875777
21	23.171842066159424	28.10876525811376	28.248462712799636	20.470929962927173
22	22.59322391627996	24.51742385021707	27.310189869284446	25.579162364218522
23	19.638613285076506	29.401250651874133	26.34877608561296	24.6113599774364
24	20.23237479047748	25.09978051599091	36.28551471792188	18.382329975609732
25	17.99565385696533	25.981171130328452	34.40920564582152	21.6139693668847
26	17.064858045238882	33.47306233667655	28.24552868766735	21.216550930417228
27	18.35914171246743	32.29410478634145	30.020424600759625	19.32632890043149
28	15.848799273876102	28.764330583236873	37.767623316603085	17.619246826283945
29	16.822422387936804	25.683367579400873	36.71004922537004	20.78416080729228
30	19.168790680021843	28.569123265967484	32.624703166570285	19.637382887440385
31	28.618859724258428	26.421700807046207	24.92388097701144	20.035558491683933
32	28.746158556611068	27.41094050648846	25.633773090067947	18.209127846832533
33	26.48170635330016	27.93584706725259	25.73476034220198	19.84768623724527
34	20.24799137585903	28.342398840018966	29.046564871768904	22.363044912353097
35	21.29231503101075	26.134828865885712	30.57036502112025	22.002491081983287
36	28.049564202621884	24.856067138066702	28.036692350428606	19.057676308882808
37	21.254361996234984	30.609217192630105	29.43849384185983	18.697926969275077
38	20.302649424694458	32.88303934716995	24.953031936390335	21.861279291745262
39	20.694057841939333	30.297784621733175	27.924252935681437	21.083904600646054
40	22.834855082819956	26.719788295889806	26.899331704791642	23.54602491649859
41	17.728941507842837	25.05028067262999	29.009558296713227	28.21121952281394
42	22.42981764560573	26.723290196854155	25.650572750099617	25.196319407440498
43	22.78322570508883	26.800190049111794	26.365149838770584	24.051434407028786
44	20.532733782649313	30.73755713067485	27.615801712902798	21.11390737377303
45	18.32450128671199	35.411885073289106	24.543120231617625	21.72049340838128
46	22.646272983591228	31.64047970364453	26.290900073729212	19.42234723903503
47	22.443967218421136	27.53028907719231	27.47099337572842	22.554750328658137
48	23.033564301053694	24.669425281264168	29.59385520491326	22.703155212768877
49	20.956369153363386	24.67164946160639	30.92708568962368	23.444895695406547
50	19.74191936352477	30.65275433975443	28.003708229183328	21.601618067537473
51	19.03392016990845	32.838839678241555	24.960083061305035	23.16715709054496
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2069.0
1	2773.0
2	3477.0
3	9580.5
4	15684.0
5	11069.0
6	6454.0
7	6788.0
8	7122.0
9	7576.0
10	8030.0
11	8372.0
12	8714.0
13	8237.0
14	7760.0
15	7379.5
16	6999.0
17	6844.5
18	6690.0
19	6555.0
20	6420.0
21	7628.5
22	8837.0
23	9559.0
24	10281.0
25	12191.0
26	15158.0
27	16215.0
28	21292.5
29	26370.0
30	30476.5
31	34583.0
32	39548.0
33	44513.0
34	49842.0
35	55171.0
36	59718.5
37	64266.0
38	76571.0
39	88876.0
40	120720.5
41	152565.0
42	179397.5
43	206230.0
44	217323.0
45	228416.0
46	216272.0
47	204128.0
48	188953.0
49	173778.0
50	160598.0
51	147418.0
52	136749.5
53	126081.0
54	116057.0
55	106033.0
56	97285.0
57	88537.0
58	79174.0
59	69811.0
60	62810.0
61	55809.0
62	47795.5
63	39782.0
64	33268.5
65	26755.0
66	21075.0
67	15395.0
68	12913.0
69	10431.0
70	8921.0
71	7411.0
72	6343.0
73	5275.0
74	4315.0
75	2617.5
76	1880.0
77	1207.0
78	534.0
79	511.0
80	488.0
81	313.0
82	138.0
83	160.0
84	182.0
85	106.0
86	30.0
87	31.0
88	32.0
89	20.5
90	9.0
91	4.5
92	0.0
93	1.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2113138.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.375520373877563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.51952425195158	14.795881583866649
2	8.005300240324086	2.9420311533016443
3	2.4780939207640054	1.3660879598814333
4	1.1907553109363536	0.875229939056555
5	0.6993334746919664	0.6425308256168408
6	0.46907149423043293	0.5171659679421908
7	0.32298436310858347	0.4154504021321951
8	0.28816912922777055	0.42362061641979637
9	0.23825711402802363	0.3940288607738896
>10	2.654475606169324	12.085901618120246
>50	1.5493908559230722	21.11424897917919
>100	1.5760249441224679	42.525581993065494
>500	0.006007387091391403	0.7501643228809587
>1k	0.002350716687935766	0.703081072673267
>5k	2.6119074310397404E-4	0.44899470508965883
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9355	0.4427065340739696	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4705	0.22265464915211405	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050257011136991527	0.0
2	0.0	0.0	0.0	0.18773028548064538	0.0
3	0.0	0.0	0.0	0.26519801357033945	0.0
4	0.0	0.0	0.0	0.3957621319573071	0.0
5	0.0	0.0	0.0	0.6826340731177992	0.0
6	0.0	0.0	0.0	0.9980417748391255	0.0
7	0.0	0.0	0.0	1.223630449123531	0.0
8	0.0	0.0	0.0	1.8256734770753258	0.0
9	0.0	0.0	0.0	2.0741191536000017	0.0
10	0.0	0.0	0.0	2.5009251643763917	0.0
11	0.0	0.0	0.0	2.8726472194433113	0.0
12	0.0	0.0	0.0	3.218436278179655	0.0
13	0.0	0.0	0.0	3.3454038496302654	0.0
14	0.0	0.0	0.0	3.3951403079212055	0.0
15	0.0	0.0	0.0	3.473791110661017	0.0
16	0.0	0.0	0.0	3.648933481864412	0.0
17	0.0	0.0	0.0	3.8548357939708624	0.0
18	0.0	0.0	0.0	4.17076404853824	0.0
19	0.0	0.0	0.0	4.307338186147804	0.0
20	0.0	0.0	0.0	4.4502536038819995	0.0
21	0.0	0.0	0.0	4.61990650870885	0.0
22	0.0	0.0	0.0	4.818994310830623	0.0
23	0.0	0.0	0.0	5.031569163963735	0.0
24	0.0	0.0	0.0	5.200796161916543	0.0
25	0.0	0.0	0.0	5.324924354206871	0.0
26	0.0	0.0	0.0	5.452933031349585	0.0
27	0.0	0.0	0.0	5.574174521493627	0.0
28	0.0	0.0	0.0	5.7054011616846605	0.0
29	0.0	0.0	0.0	5.856503455997668	0.0
30	0.0	0.0	0.0	6.041914915164083	0.0
31	0.0	0.0	0.0	6.210053484438783	0.0
32	0.0	0.0	0.0	6.3667398911003446	0.0
33	0.0	0.0	0.0	6.517463601525314	0.0
34	4.732298600470012E-5	0.0	0.0	6.691328252106583	0.0
35	4.732298600470012E-5	0.0	0.0	6.897419856157052	0.0
36	4.732298600470012E-5	0.0	0.0	7.067593313829954	0.0
37	9.464597200940024E-5	0.0	0.0	7.237577479558836	0.0
38	9.464597200940024E-5	0.0	0.0	7.403491868491315	0.0
39	9.464597200940024E-5	0.0	0.0	7.580006606288846	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCAGCG	55	1.8189894E-12	45.000004	1
CGTACCA	35	1.2125747E-7	45.000004	32
CGGTCGA	35	1.2125747E-7	45.000004	44
CTATGCG	50	2.1827873E-11	45.0	1
GGTCGAC	25	3.89192E-5	45.0	9
ACACCGT	20	7.0346333E-4	45.0	45
CCGTCGA	25	3.89192E-5	45.0	45
TACGCTA	40	6.8193913E-9	45.0	42
GTTCGAC	25	3.89192E-5	45.0	39
CGTCGGT	20	7.0346333E-4	45.0	36
CGCACGA	25	3.89192E-5	45.0	39
ATCGGAT	20	7.0346333E-4	45.0	36
CGACTAT	25	3.89192E-5	45.0	42
TATCGGC	20	7.0346333E-4	45.0	38
ACGCGCC	25	3.89192E-5	45.0	18
ATCTCGC	30	2.166371E-6	44.999996	10
CCGTAGA	30	2.166371E-6	44.999996	24
TCGCTAG	30	2.166371E-6	44.999996	1
TACCGGT	60	0.0	44.999996	40
CGGTCTA	230	0.0	44.02174	31
>>END_MODULE