##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549227_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1776568
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99380828653899	33.0	31.0	34.0	30.0	34.0
2	32.3893467629722	34.0	31.0	34.0	31.0	34.0
3	32.461234807786695	34.0	31.0	34.0	31.0	34.0
4	36.05715570695858	37.0	35.0	37.0	35.0	37.0
5	36.01898660788667	37.0	35.0	37.0	35.0	37.0
6	36.06904604833589	37.0	35.0	37.0	35.0	37.0
7	36.26261589761833	37.0	36.0	37.0	35.0	37.0
8	36.13046559433695	37.0	36.0	37.0	35.0	37.0
9	37.93541198535603	39.0	39.0	39.0	35.0	39.0
10	37.71935045548496	39.0	38.0	39.0	35.0	39.0
11	37.51786253045197	39.0	37.0	39.0	35.0	39.0
12	36.90270285179064	39.0	35.0	39.0	34.0	39.0
13	36.7339206830248	39.0	35.0	39.0	33.0	39.0
14	37.891817256643144	40.0	35.0	41.0	33.0	41.0
15	38.021090664697326	40.0	35.0	41.0	33.0	41.0
16	38.09646126689212	40.0	35.0	41.0	34.0	41.0
17	38.02104788558614	40.0	35.0	41.0	34.0	41.0
18	37.97444679854641	40.0	36.0	41.0	34.0	41.0
19	37.922342404005924	40.0	36.0	41.0	34.0	41.0
20	37.800627952321555	40.0	35.0	41.0	34.0	41.0
21	37.674578738331434	40.0	35.0	41.0	34.0	41.0
22	37.62540189849192	40.0	35.0	41.0	33.0	41.0
23	37.54757656335136	39.0	35.0	41.0	33.0	41.0
24	37.52892824817288	39.0	35.0	41.0	33.0	41.0
25	37.417683421068034	39.0	35.0	41.0	33.0	41.0
26	37.35484597268441	39.0	35.0	41.0	33.0	41.0
27	37.29661515911578	39.0	35.0	41.0	33.0	41.0
28	37.22159692170522	39.0	35.0	41.0	33.0	41.0
29	37.13938109883776	39.0	35.0	41.0	33.0	41.0
30	37.09098554066042	39.0	35.0	41.0	33.0	41.0
31	36.93797197743064	39.0	35.0	41.0	33.0	41.0
32	36.809873869167966	39.0	35.0	41.0	32.0	41.0
33	36.71702293410666	39.0	35.0	41.0	32.0	41.0
34	36.57824243147462	39.0	35.0	41.0	31.0	41.0
35	36.477845486353466	39.0	35.0	41.0	31.0	41.0
36	36.364623814005434	39.0	35.0	41.0	31.0	41.0
37	36.10784557641475	38.0	35.0	41.0	30.0	41.0
38	36.19320566395432	38.0	35.0	41.0	31.0	41.0
39	36.09610665057572	38.0	35.0	41.0	30.0	41.0
40	35.96163164033125	38.0	35.0	41.0	30.0	41.0
41	35.89612894074418	38.0	35.0	41.0	30.0	41.0
42	35.88407423751863	38.0	35.0	41.0	30.0	41.0
43	35.79172483124766	38.0	35.0	40.0	30.0	41.0
44	35.66374267689162	38.0	35.0	40.0	30.0	41.0
45	35.593848363811574	37.0	35.0	40.0	30.0	41.0
46	35.422263600380056	37.0	35.0	40.0	29.0	41.0
47	35.39119864818009	37.0	35.0	40.0	29.0	41.0
48	35.25476367918368	37.0	35.0	40.0	29.0	41.0
49	35.20582719040307	36.0	35.0	40.0	29.0	41.0
50	35.08867997172076	36.0	34.0	40.0	29.0	41.0
51	34.57719659478275	35.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	8.0
12	7.0
13	11.0
14	27.0
15	46.0
16	105.0
17	206.0
18	475.0
19	942.0
20	1677.0
21	2778.0
22	4295.0
23	6284.0
24	9356.0
25	13840.0
26	19154.0
27	22638.0
28	24069.0
29	25760.0
30	29235.0
31	35543.0
32	45046.0
33	61577.0
34	122084.0
35	263679.0
36	111543.0
37	151834.0
38	250658.0
39	573331.0
40	352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.732979542578725	23.23930184490546	28.32703279581755	13.700685816698263
2	32.786361118741304	25.228699379928045	29.047410512854	12.937528988476657
3	31.116399710002657	24.88258259745757	30.25760905296054	13.743408639579233
4	27.86226026811245	26.875075989210657	29.725290560226235	15.53737318245066
5	23.889262893398957	32.16668317790256	28.31965902796853	15.624394900729946
6	21.60170621107664	40.995053383827695	26.521022555849257	10.882217849246413
7	85.41080330164678	5.341591202813515	7.021177911568823	2.2264275839708922
8	86.08429286129211	4.547081789157522	6.664366351302061	2.704258998248308
9	80.94578985999973	6.733994983586331	8.568036799041748	3.752178357372192
10	47.562266122096084	25.671632045607033	13.709241638935296	13.056860193361583
11	42.04252243651805	22.881364518554875	19.703214287322524	15.37289875760455
12	37.90859680012248	21.585832909294776	24.525602172278234	15.979968118304505
13	24.651856838578652	32.888017796110255	24.513669051789744	17.94645631352135
14	17.62262969951052	36.913757311850716	27.000767772469164	18.462845216169605
15	17.125491396895587	24.198060530190794	40.76472164307812	17.911726429835504
16	19.372464211896194	19.538345844347077	40.531575487118985	20.557614456637744
17	19.1653795407775	20.606247551458768	28.802837831144096	31.42553507661964
18	23.275495224500272	24.721260317646156	29.3401659829514	22.66307847490217
19	29.735985338022523	25.664427142670586	24.17751529916108	20.42207222014581
20	31.026394711601245	24.30894848944707	23.112202854042177	21.552453944909512
21	26.035029337464145	26.939976403942882	24.947257858973032	22.07773639961994
22	24.14937114706558	25.56018120330885	24.420680773266206	25.869766876359364
23	24.001952078389344	29.19770028504397	23.98101282923029	22.819334807336393
24	23.38435680480567	25.31414502568998	30.817396238140056	20.484101931364293
25	22.610561487091964	25.5223554628925	29.56858392135848	22.298499128657053
26	20.339947584331135	31.339582836119977	25.390697119389742	22.92977246015914
27	22.47361204299526	28.943051996883877	26.77353188845009	21.809804071670772
28	19.12006745590374	28.563049655290424	32.91846976867758	19.398413120128247
29	20.38345844347078	26.93980753903031	31.40712880114918	21.269605216349728
30	21.899696493463804	27.212186643010565	29.116363685487972	21.771753178037653
31	28.670954334424575	26.362796132768352	22.919133970667037	22.047115562140036
32	29.196067924222437	27.347391149677357	24.496275965794723	18.960264960305487
33	26.712571655011235	27.300784433807205	24.20453368517276	21.7821102260088
34	21.68670155040505	28.308513943738717	27.474884158669976	22.52990034718626
35	23.141135042396353	25.720096275515488	27.154265977998026	23.984502704090133
36	27.794601726474866	27.646676063060916	23.916112414498066	20.642609795966155
37	23.575117867708975	30.311927266504856	25.089554691967887	21.02340017381828
38	23.92798924668237	30.52723003003544	22.437249798487873	23.10753092479432
39	23.80792629384296	28.541322369872695	24.25339193321055	23.397359403073793
40	24.044055729924214	26.069421491324846	26.137755492612726	23.748767286138218
41	20.21718279289056	25.43645951069703	26.691069522810274	27.655288173602138
42	23.375744694264448	27.783287777332475	23.631856478333507	25.20911105006957
43	24.044280883140978	27.759196383138722	23.772408373898436	24.424114359821857
44	22.76259619671186	29.803362438139153	25.623111527394393	21.810929837754593
45	21.044902306019246	30.687201390546264	23.349289191294677	24.918607112139814
46	23.030190794835885	30.815088417668225	25.23140121852921	20.92331956896668
47	25.4328570592288	26.821376947012443	26.246842226134888	21.498923767623868
48	24.52256260385192	24.97421995668052	27.0512583813285	23.451959058139064
49	21.938760576572356	26.27065217880768	27.99763363969181	23.792953604928154
50	20.378448784397783	29.485389807764186	26.412554993673194	23.723606414164838
51	21.393158044048977	30.304328345439068	24.524532694498607	23.777980916013348
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1124.0
1	2186.0
2	3248.0
3	9012.5
4	14777.0
5	10630.5
6	6484.0
7	6609.5
8	6735.0
9	7035.0
10	7335.0
11	7297.0
12	7259.0
13	7184.5
14	7110.0
15	6848.0
16	6586.0
17	6165.0
18	5744.0
19	5488.5
20	5233.0
21	5487.5
22	5742.0
23	5670.5
24	5599.0
25	7440.0
26	11422.0
27	13563.0
28	16489.0
29	19415.0
30	23651.5
31	27888.0
32	29815.0
33	31742.0
34	31857.0
35	31972.0
36	37481.5
37	42991.0
38	46829.5
39	50668.0
40	72818.0
41	94968.0
42	111710.0
43	128452.0
44	129253.5
45	130055.0
46	132309.0
47	134563.0
48	131527.5
49	128492.0
50	130456.5
51	132421.0
52	130229.5
53	128038.0
54	117518.5
55	106999.0
56	104090.0
57	101181.0
58	99558.0
59	97935.0
60	91334.0
61	84733.0
62	75583.0
63	66433.0
64	54777.0
65	43121.0
66	35286.5
67	27452.0
68	23731.5
69	20011.0
70	18037.5
71	16064.0
72	13593.5
73	11123.0
74	9107.0
75	5163.0
76	3235.0
77	2731.0
78	2227.0
79	1573.5
80	920.0
81	628.0
82	336.0
83	220.5
84	105.0
85	82.5
86	60.0
87	44.5
88	29.0
89	22.0
90	15.0
91	10.0
92	5.0
93	4.5
94	4.0
95	3.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1776568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.828382708794344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.3150085002633	15.925167258032912
2	6.1960084522218235	2.457136537151576
3	2.0111836718131353	1.1963555862716726
4	1.147581471844693	0.9101873845303229
5	0.7377052687732515	0.731375119776501
6	0.5757129268727762	0.6849273746660122
7	0.4514658927270754	0.6266286950671959
8	0.37213780720046546	0.5903112689265881
9	0.3151379976492775	0.5623809141125706
>10	4.918222456893283	25.037707092399724
>50	2.366489191708011	33.38369497742359
>100	0.5818418276269225	15.335347130154627
>500	0.0077655607240659135	1.0305154353451333
>1k	0.003451360321807073	1.0816134890290978
>5k	2.876133601505894E-4	0.44665173711251155
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7832	0.44084999842392747	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2735	0.1539485119623904	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2586	0.1455615546379311	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1845	0.10385192123239864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1257660838200396E-4	0.0	0.0	0.01902544681655867	0.0
2	1.1257660838200396E-4	0.0	0.0	0.06383093695259624	0.0
3	1.1257660838200396E-4	0.0	0.0	0.09856082063844446	0.0
4	1.1257660838200396E-4	0.0	0.0	0.15856415290605258	0.0
5	1.1257660838200396E-4	0.0	0.0	0.284931395814852	0.0
6	1.1257660838200396E-4	0.0	0.0	0.4921849318461213	0.0
7	1.1257660838200396E-4	0.0	0.0	0.6419118209941865	0.0
8	1.1257660838200396E-4	0.0	0.0	1.0676202655907345	0.0
9	1.1257660838200396E-4	0.0	0.0	1.312361812213211	0.0
10	1.1257660838200396E-4	0.0	0.0	1.6930396134569574	0.0
11	1.1257660838200396E-4	0.0	0.0	1.9209509571263244	0.0
12	1.1257660838200396E-4	0.0	0.0	2.128879952807886	0.0
13	1.1257660838200396E-4	0.0	0.0	2.218716086296725	0.0
14	1.1257660838200396E-4	0.0	0.0	2.2516447442484613	0.0
15	1.1257660838200396E-4	0.0	0.0	2.2941987022168586	0.0
16	1.6886491257300592E-4	0.0	0.0	2.3727771748674975	0.0
17	1.6886491257300592E-4	0.0	0.0	2.4650337054365496	0.0
18	1.6886491257300592E-4	0.0	0.0	2.620333136699524	0.0
19	1.6886491257300592E-4	0.0	0.0	2.6843329385646935	0.0
20	1.6886491257300592E-4	0.0	0.0	2.766964169117084	0.0
21	1.6886491257300592E-4	0.0	0.0	2.8696903242656626	0.0
22	1.6886491257300592E-4	0.0	0.0	2.9767506788369484	0.0
23	1.6886491257300592E-4	0.0	0.0	3.094674676117098	0.0
24	1.6886491257300592E-4	0.0	0.0	3.1789945557952186	0.0
25	1.6886491257300592E-4	0.0	0.0	3.250368125509409	0.0
26	1.6886491257300592E-4	0.0	0.0	3.3191524332308138	0.0
27	1.6886491257300592E-4	0.0	0.0	3.389231371948611	0.0
28	1.6886491257300592E-4	0.0	0.0	3.4655020241274186	0.0
29	1.6886491257300592E-4	0.0	0.0	3.5470074885959897	0.0
30	1.6886491257300592E-4	0.0	0.0	3.669096820386273	0.0
31	1.6886491257300592E-4	0.0	0.0	3.7675450644163355	0.0
32	1.6886491257300592E-4	0.0	0.0	3.868582570439184	0.0
33	1.6886491257300592E-4	0.0	0.0	3.9680440039446845	0.0
34	1.6886491257300592E-4	0.0	0.0	4.066154518149601	0.0
35	1.6886491257300592E-4	0.0	0.0	4.178337108402268	0.0
36	1.6886491257300592E-4	0.0	0.0	4.2904071220465525	0.0
37	1.6886491257300592E-4	0.0	0.0	4.403546613470467	0.0
38	1.6886491257300592E-4	0.0	0.0	4.511057274475281	0.0
39	1.6886491257300592E-4	0.0	0.0	4.635961021475114	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATC	45	3.8562575E-10	45.000004	33
GGTACGA	25	3.891669E-5	45.0	8
CCCGTCA	40	6.8193913E-9	45.0	44
TATTACC	20	7.034327E-4	45.0	37
GCGATCG	20	7.034327E-4	45.0	9
AATTACG	35	1.2124292E-7	45.0	1
CACGTTA	20	7.034327E-4	45.0	45
CGGTAAT	25	3.891669E-5	45.0	6
ACTAACG	30	2.1661745E-6	44.999996	1
TAGCCGT	85	0.0	42.35294	44
CGTTTTT	5425	0.0	41.35023	1
GTTCACG	55	6.184564E-11	40.909092	1
TACGCGG	165	0.0	40.90909	2
ACATACG	575	0.0	40.695652	17
TTTAGCG	100	0.0	40.5	1
CGCGATA	50	1.0822987E-9	40.5	11
TCGTAAT	50	1.0822987E-9	40.5	32
TATTACG	90	0.0	40.000004	1
CGGTATA	40	3.459645E-7	39.375	35
CATACGA	590	0.0	39.279663	18
>>END_MODULE