##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549226_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1964267 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.61230881545126 31.0 31.0 33.0 30.0 34.0 2 31.98397264730304 31.0 31.0 34.0 30.0 34.0 3 32.04902897620334 33.0 31.0 34.0 30.0 34.0 4 35.749609396278615 37.0 35.0 37.0 35.0 37.0 5 35.663101808460866 37.0 35.0 37.0 33.0 37.0 6 35.72224957197774 37.0 35.0 37.0 33.0 37.0 7 36.065439168911354 37.0 35.0 37.0 35.0 37.0 8 35.96821969722039 37.0 35.0 37.0 35.0 37.0 9 37.75933363437863 39.0 38.0 39.0 35.0 39.0 10 37.429768458157675 39.0 37.0 39.0 35.0 39.0 11 37.07510129732872 39.0 37.0 39.0 33.0 39.0 12 35.96004972847378 37.0 35.0 39.0 32.0 39.0 13 35.57006150385869 37.0 35.0 39.0 31.0 39.0 14 36.46285102788979 38.0 35.0 41.0 31.0 41.0 15 36.76624104564196 38.0 35.0 41.0 32.0 41.0 16 36.93435668368913 38.0 35.0 41.0 33.0 41.0 17 36.85386762593884 38.0 35.0 41.0 32.0 41.0 18 36.78169006555626 38.0 35.0 40.0 32.0 41.0 19 36.71883608491106 37.0 35.0 40.0 32.0 41.0 20 36.59839217377271 37.0 35.0 40.0 32.0 41.0 21 36.43421897328622 37.0 35.0 40.0 32.0 41.0 22 36.356744780623 37.0 35.0 40.0 31.0 41.0 23 36.31193366278617 37.0 35.0 40.0 31.0 41.0 24 36.29419727562495 36.0 35.0 40.0 32.0 41.0 25 36.17628153402771 36.0 35.0 40.0 31.0 41.0 26 36.09611931575493 36.0 35.0 40.0 31.0 41.0 27 35.9994247217919 36.0 35.0 40.0 31.0 41.0 28 36.033615083896436 36.0 35.0 40.0 31.0 41.0 29 36.01476072244761 36.0 35.0 40.0 31.0 41.0 30 36.06841788819952 36.0 35.0 40.0 31.0 41.0 31 35.89440335758835 36.0 35.0 40.0 31.0 41.0 32 35.672992520874196 36.0 35.0 40.0 30.0 41.0 33 35.60325098370028 36.0 35.0 40.0 30.0 41.0 34 35.45670929664857 36.0 35.0 40.0 30.0 41.0 35 35.357633152723125 36.0 34.0 40.0 30.0 41.0 36 35.23093398198921 36.0 34.0 40.0 29.0 41.0 37 34.922906101869046 35.0 34.0 40.0 27.0 41.0 38 35.05368007506108 35.0 34.0 40.0 29.0 41.0 39 34.999346321044946 36.0 34.0 40.0 28.0 41.0 40 34.82805799822529 36.0 34.0 40.0 27.0 41.0 41 34.830767914952496 35.0 34.0 40.0 27.0 41.0 42 34.83422111148841 35.0 34.0 40.0 27.0 41.0 43 34.722802449972434 35.0 34.0 40.0 27.0 41.0 44 34.601856061319566 35.0 34.0 40.0 27.0 41.0 45 34.580553967459615 35.0 34.0 40.0 27.0 41.0 46 34.40434167045519 35.0 34.0 40.0 27.0 41.0 47 34.290564368285985 35.0 34.0 40.0 26.0 41.0 48 34.182231845263395 35.0 34.0 39.0 26.0 41.0 49 34.260181024270125 35.0 34.0 39.0 26.0 41.0 50 34.12281477008981 35.0 34.0 39.0 26.0 41.0 51 33.52024699289862 35.0 33.0 38.0 24.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 6.0 12 8.0 13 7.0 14 12.0 15 50.0 16 138.0 17 312.0 18 781.0 19 1468.0 20 2777.0 21 4413.0 22 6902.0 23 9940.0 24 14353.0 25 20450.0 26 27238.0 27 32283.0 28 35801.0 29 40307.0 30 47435.0 31 58517.0 32 74036.0 33 99838.0 34 196893.0 35 377462.0 36 117798.0 37 146693.0 38 225632.0 39 422552.0 40 162.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.630051820857346 24.743835741271425 31.02409193862138 13.602020499249848 2 29.521292166492643 26.101594131551366 30.464952066088774 13.912161635867221 3 28.607516187972408 25.130443060948437 32.4095960477878 13.852444703291356 4 26.44457194464907 27.55704799805729 31.24066127466378 14.757718782629855 5 23.94643905334662 32.06672005384197 29.4109711154339 14.575869777377514 6 21.7692401287605 39.98107181966606 26.977697023877102 11.271991027696336 7 87.08261147797117 4.916388657957396 6.102887234780201 1.8981126292912316 8 89.10453619594485 3.824938259411781 5.427571709955928 1.6429538346874433 9 85.50634918776318 5.138965323960541 6.647976064353776 2.7067094239225113 10 57.711451650921184 22.11501796853483 11.211663180209207 8.961867200334781 11 52.07657614774366 18.71955289174028 17.492479382894484 11.711391577621576 12 46.32109585916782 20.19878153020949 20.965887020450886 12.514235590171804 13 22.48003962801391 42.783033060169515 21.320828583894144 13.416098727922426 14 14.404813602224136 44.90367144588795 26.06346285917342 14.628052092714483 15 13.437328021088781 22.14933102271738 50.87857200675876 13.534768949435083 16 15.527013384636609 16.936444994494128 48.60601944643982 18.93052217442944 17 16.12779729028691 17.989764120661807 27.70392212463988 38.1785164644114 18 22.52733462406078 24.437767370729134 32.58996867533793 20.44492932987216 19 30.26564107628953 24.37102491667375 24.29140233990593 21.07193166713079 20 31.782135524345723 23.355175238396818 24.096113206605825 20.76657603065164 21 22.935934880543225 28.10549685964281 27.12925483144603 21.829313428367936 22 23.291843725929315 24.615289062026697 25.068537016607213 27.024330195436768 23 20.2671530906949 30.264724703922635 23.968686538031744 25.499435667350724 24 21.54371070735292 23.12725306692013 37.58170350568431 17.747332720042643 25 17.22764776886238 25.31646665142773 35.00684988344253 22.449035696267362 26 16.86008063058637 36.183421092957325 26.39483328895715 20.561664987499153 27 18.893205455266518 33.675513563074674 29.26144969090251 18.169831290756296 28 14.461628688971508 28.78020146955582 39.97358811200311 16.78458172946957 29 15.070049031012585 25.613524027028912 37.80433108126339 21.51209586069511 30 18.91397656224943 29.183048943957214 32.25915825088952 19.643816242903842 31 30.35330736605563 25.934916179928695 23.61730864490418 20.094467809111492 32 30.406660601639185 28.084267566476452 24.964885119996417 16.544186711887946 33 27.243343191124218 28.71610631344924 24.37754134239388 19.663009153032657 34 19.582826570929512 28.441398241685068 28.867104115682846 23.108671071702574 35 20.634262042787462 25.758870866333343 30.67923047121394 22.927636619665247 36 30.442195485644262 24.26248570077286 26.828328328073525 18.466990485509353 37 20.82435839934184 31.344313171274578 29.024312886180955 18.807015543202628 38 21.364101723441877 31.728324102578725 22.616171834073473 24.29140233990593 39 21.390523793353957 30.30942331159664 26.403691555170454 21.896361339878947 40 23.466259933094634 26.074815694607707 24.928077496592877 25.53084687570478 41 16.63266755486907 24.25148923237014 27.31741662411475 31.798426588646045 42 23.70991316353632 26.148634579718543 23.358484360832822 26.782967895912318 43 23.303400199667358 27.443621462866304 24.07233843464254 25.180639902823803 44 20.182897742516673 31.633581381757164 27.01277372169873 21.17074715402743 45 18.45390672449316 36.89590060821671 21.909648739198897 22.740543928091242 46 22.82205015916879 32.677787693831846 25.469653565426697 19.03050858157267 47 24.35071199587429 27.156084177965624 26.118903387370455 22.374300438789636 48 25.13533038023853 22.935476694359778 29.60381658908896 22.325376336312733 49 21.78217116104888 23.02019022872145 31.344414990426454 23.853223619803217 50 18.788688095864767 32.803228888944325 26.41234618308 21.99573683211091 51 19.641067125803165 33.38278350142827 23.54613705774215 23.43001231502642 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1544.0 1 2296.0 2 3048.0 3 8209.0 4 13370.0 5 9883.5 6 6397.0 7 6748.0 8 7099.0 9 7662.5 10 8226.0 11 8253.0 12 8280.0 13 7922.0 14 7564.0 15 7174.0 16 6784.0 17 6527.5 18 6271.0 19 5814.5 20 5358.0 21 5213.0 22 5068.0 23 5387.0 24 5706.0 25 7411.5 26 12743.0 27 16369.0 28 19130.0 29 21891.0 30 24294.5 31 26698.0 32 29978.5 33 33259.0 34 37129.0 35 40999.0 36 42716.0 37 44433.0 38 57192.5 39 69952.0 40 101455.5 41 132959.0 42 168484.0 43 204009.0 44 205918.0 45 207827.0 46 192101.0 47 176375.0 48 163668.0 49 150961.0 50 152882.0 51 154803.0 52 145785.0 53 136767.0 54 120900.5 55 105034.0 56 94141.5 57 83249.0 58 78983.0 59 74717.0 60 70140.0 61 65563.0 62 54173.0 63 42783.0 64 36729.0 65 30675.0 66 24708.5 67 18742.0 68 15914.0 69 13086.0 70 10655.5 71 8225.0 72 6439.5 73 4654.0 74 4211.5 75 2667.0 76 1565.0 77 1113.5 78 662.0 79 416.5 80 171.0 81 139.0 82 107.0 83 90.0 84 73.0 85 45.0 86 17.0 87 16.0 88 15.0 89 14.0 90 13.0 91 10.5 92 8.0 93 6.0 94 4.0 95 2.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1964267.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.591401292512366 #Duplication Level Percentage of deduplicated Percentage of total 1 82.76358181604024 14.559273801316433 2 7.368241442858955 2.5923538408290443 3 2.0816594937554824 1.0985792252706248 4 0.9778775147877481 0.6880894311022387 5 0.5373760752773897 0.47265990925999474 6 0.3860778077257733 0.40749897875025054 7 0.2590186671917697 0.3189550921775489 8 0.1962968634888318 0.2762509518474847 9 0.16904181718456646 0.26763141971782994 >10 2.151064520620515 9.56555890820836 >50 1.2843921395774511 16.76861103375732 >100 1.8151921068751409 50.933300903479775 >500 0.007852938132604813 0.8471308473576491 >1k 0.0017450973628010693 0.5054030627649247 >5k 5.816991209336898E-4 0.6987025941605397 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7551 0.38441820791165354 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 6105 0.3108029611045749 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 2223 0.1131719873112973 No Hit AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 2193 0.11164470003314213 No Hit GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 2089 0.10635010413553758 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.029323915740579055 0.0 2 0.0 0.0 0.0 0.11984114175924149 0.0 3 0.0 0.0 0.0 0.16540521222420373 0.0 4 0.0 0.0 0.0 0.23968228351848297 0.0 5 0.0 0.0 0.0 0.4085493469065051 0.0 6 0.0 0.0 0.0 0.5833728306793323 0.0 7 5.0909575938505305E-5 0.0 0.0 0.7180286590366788 0.0 8 5.0909575938505305E-5 0.0 0.0 1.055457328357092 0.0 9 5.0909575938505305E-5 0.0 0.0 1.2276844237570554 0.0 10 5.0909575938505305E-5 0.0 0.0 1.5416437785698176 0.0 11 5.0909575938505305E-5 0.0 0.0 1.8397193456897663 0.0 12 5.0909575938505305E-5 0.0 0.0 2.100529103222729 0.0 13 5.0909575938505305E-5 0.0 0.0 2.2168574842422135 0.0 14 5.0909575938505305E-5 0.0 0.0 2.258705155663665 0.0 15 5.0909575938505305E-5 0.0 0.0 2.318880274422978 0.0 16 1.0181915187701061E-4 0.0 0.0 2.4449832940226557 0.0 17 1.0181915187701061E-4 0.0 0.0 2.5884973885933023 0.0 18 1.0181915187701061E-4 0.0 0.0 2.8169286558293756 0.0 19 1.0181915187701061E-4 0.0 0.0 2.9059695041458213 0.0 20 1.5272872781551592E-4 0.0 0.0 3.0059050017131073 0.0 21 1.5272872781551592E-4 0.0 0.0 3.1396444577035605 0.0 22 1.5272872781551592E-4 0.0 0.0 3.2746566530924768 0.0 23 1.5272872781551592E-4 0.0 0.0 3.424992630838883 0.0 24 1.5272872781551592E-4 0.0 0.0 3.524419032646784 0.0 25 1.5272872781551592E-4 0.0 0.0 3.608165285065625 0.0 26 1.5272872781551592E-4 0.0 0.0 3.69247154281979 0.0 27 2.0363830375402122E-4 0.0 0.0 3.7731632206823207 0.0 28 2.0363830375402122E-4 0.0 0.0 3.859862228505595 0.0 29 2.0363830375402122E-4 0.0 0.0 3.9521103801061668 0.0 30 2.0363830375402122E-4 0.0 0.0 4.083355266875634 0.0 31 2.0363830375402122E-4 0.0 0.0 4.204978243792723 0.0 32 2.0363830375402122E-4 0.0 0.0 4.3052701083915785 0.0 33 2.0363830375402122E-4 0.0 0.0 4.401896483522862 0.0 34 2.0363830375402122E-4 0.0 0.0 4.509926603664369 0.0 35 2.5454787969252655E-4 0.0 0.0 4.6587352941326206 0.0 36 2.5454787969252655E-4 0.0 0.0 4.773740026177704 0.0 37 2.5454787969252655E-4 0.0 0.0 4.895617550974485 0.0 38 2.5454787969252655E-4 0.0 0.0 5.00512404881821 0.0 39 2.5454787969252655E-4 0.0 0.0 5.12231789262865 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACGTA 95 0.0 45.000004 23 TCCGCGA 20 7.034508E-4 45.000004 45 TACTTCG 20 7.034508E-4 45.000004 29 GATCGAC 20 7.034508E-4 45.000004 9 TCCGATT 20 7.034508E-4 45.000004 28 TAGACGC 20 7.034508E-4 45.000004 23 ACTAGTC 20 7.034508E-4 45.000004 12 CTAACGA 20 7.034508E-4 45.000004 18 TGCGCAT 20 7.034508E-4 45.000004 1 CTACGTT 40 6.8193913E-9 45.000004 30 AATTACG 20 7.034508E-4 45.000004 1 ACGTAAG 80 0.0 45.000004 1 ATCGATC 20 7.034508E-4 45.000004 36 CGAATCG 35 1.2125201E-7 45.0 35 CGTACAC 25 3.8918202E-5 45.0 17 CCGAATA 25 3.8918202E-5 45.0 12 ACGTAGT 25 3.8918202E-5 45.0 44 CGACTCA 35 1.2125201E-7 45.0 34 TATCGCG 50 2.1827873E-11 45.0 1 TGGTCGA 35 1.2125201E-7 45.0 34 >>END_MODULE