FastQCFastQC Report
Sat 18 Jun 2016
SRR3549223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039466
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA64190.3147392503724014No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35250.1728393608915275No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34930.17127032272173207No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT207.857919E-444.03
ACGGATA351.4468242E-744.014
TCGCTAA207.857919E-444.036
GAACGAT408.3182385E-944.09
TTACGCG254.444058E-544.035
TATCGTG502.7284841E-1144.01
TCTATCG207.857919E-444.042
TATGCGA1100.042.02
CTATCTC14600.040.9862986
GAGCGAT19200.040.9062547
CGGTCTA3250.040.61538331
CGACGGT3350.040.05970428
CGTTTTT46600.040.0343361
CACGACC1550.039.74193227
TCGATAG1000.039.61
GACGGTC3400.039.47058529
AGACCGA6950.039.25188
TATCTCT15250.039.2393467
AGGGGAA146500.038.9542668
AGCGATA4700.038.8510678
Produced by FastQC (version 0.11.5)