##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549223_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2039466
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.494622611997453	31.0	31.0	33.0	30.0	34.0
2	31.85546902963815	31.0	31.0	34.0	30.0	34.0
3	31.983819293873985	31.0	31.0	34.0	30.0	34.0
4	35.65788299486238	37.0	35.0	37.0	33.0	37.0
5	35.593762288755975	37.0	35.0	37.0	33.0	37.0
6	35.63299314624514	37.0	35.0	37.0	33.0	37.0
7	35.99365422125204	37.0	35.0	37.0	35.0	37.0
8	18.36925891385294	29.0	0.0	37.0	0.0	37.0
9	27.85411083097242	28.0	17.0	39.0	17.0	39.0
10	33.56723279525131	32.0	32.0	39.0	27.0	39.0
11	35.75695549717426	37.0	35.0	39.0	32.0	39.0
12	36.39897306451787	37.0	35.0	39.0	32.0	39.0
13	36.50038735629817	39.0	35.0	39.0	33.0	39.0
14	37.61282267024799	39.0	36.0	41.0	33.0	41.0
15	37.88942252530809	40.0	36.0	41.0	33.0	41.0
16	38.00679491592407	40.0	36.0	41.0	33.0	41.0
17	37.97967163953702	40.0	36.0	41.0	33.0	41.0
18	37.9790876631432	40.0	36.0	41.0	33.0	41.0
19	37.97907932762792	40.0	36.0	41.0	33.0	41.0
20	37.88765392509608	40.0	36.0	41.0	33.0	41.0
21	37.73537190617544	40.0	36.0	41.0	33.0	41.0
22	37.73219705550375	40.0	36.0	41.0	33.0	41.0
23	37.62528867850702	39.0	35.0	41.0	33.0	41.0
24	37.51152115308615	39.0	35.0	41.0	33.0	41.0
25	37.371521761088445	39.0	35.0	41.0	32.0	41.0
26	37.30670430396977	39.0	35.0	41.0	33.0	41.0
27	37.18472188308116	39.0	35.0	41.0	32.0	41.0
28	37.22269260679021	39.0	35.0	41.0	32.0	41.0
29	37.16816853038982	39.0	35.0	41.0	32.0	41.0
30	37.09070854821801	39.0	35.0	41.0	32.0	41.0
31	37.137899822796754	39.0	35.0	41.0	32.0	41.0
32	36.96699234015178	39.0	35.0	41.0	32.0	41.0
33	36.90770819420378	39.0	35.0	41.0	31.0	41.0
34	36.830067772642444	39.0	35.0	41.0	31.0	41.0
35	36.70905472314812	39.0	35.0	41.0	31.0	41.0
36	36.541254426403775	39.0	35.0	41.0	31.0	41.0
37	36.46156690035529	39.0	35.0	41.0	30.0	41.0
38	36.36812822572183	39.0	35.0	41.0	30.0	41.0
39	36.25911684725315	39.0	35.0	40.0	30.0	41.0
40	36.163386886567366	38.0	35.0	40.0	30.0	41.0
41	36.13828717909492	38.0	35.0	40.0	30.0	41.0
42	35.983684944980695	38.0	35.0	40.0	30.0	41.0
43	35.981088186809686	38.0	35.0	40.0	30.0	41.0
44	35.763739135636484	38.0	35.0	40.0	29.0	41.0
45	35.68390451225958	38.0	35.0	40.0	29.0	41.0
46	35.6805673642022	38.0	35.0	40.0	29.0	41.0
47	35.56301943744098	38.0	35.0	40.0	29.0	41.0
48	35.54886524217614	38.0	35.0	40.0	29.0	41.0
49	35.52547970890419	38.0	35.0	40.0	28.0	41.0
50	34.87843631617296	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	0.0
13	7.0
14	12.0
15	28.0
16	95.0
17	196.0
18	519.0
19	1058.0
20	2055.0
21	3612.0
22	5634.0
23	8361.0
24	11859.0
25	15924.0
26	20498.0
27	24393.0
28	28938.0
29	35502.0
30	45475.0
31	59543.0
32	78409.0
33	105848.0
34	162291.0
35	253901.0
36	218479.0
37	319166.0
38	353853.0
39	283807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.80966684416411	24.832382594267322	29.6237838728373	12.734166688731266
2	28.828085390979798	27.534805679525913	29.862277674646204	13.774831254848083
3	29.62907937666036	26.097076391565246	30.857587231167372	13.41625700060702
4	25.688194851005115	28.97415303809919	29.93239406785894	15.405258043036756
5	23.879044808788183	32.7823067410783	28.537470102468	14.801178347665516
6	21.608793674422618	41.310519518344506	25.52123938325032	11.559447423982553
7	85.15469245380899	5.0919211205286095	7.2436608406318115	2.5097255850305915
8	43.19179628392922	51.099895757026594	3.186324263312063	2.521983695732118
9	78.9502252060098	5.913312602416515	10.325104708781613	4.811357482792064
10	46.94057169867014	24.271794675665102	14.086824688423341	14.700808937241415
11	39.74172651076311	22.701873921899164	22.349820982551314	15.20657858478641
12	35.57524371575697	20.74008588522682	25.160213506868956	18.52445689214726
13	22.992293080639737	33.33769722074308	27.002852707522457	16.66715699109473
14	16.913839210852252	36.66096909681259	27.703820509878568	18.721371182456583
15	14.81525065875087	23.75592434490205	44.10232874683863	17.32649624950845
16	16.696429359449976	19.393213713785865	42.780266991457566	21.130089935306597
17	17.018719606014514	22.124811102514087	29.098891572597925	31.75757771887347
18	21.110329860855735	23.326204016149326	32.610300931714484	22.953165191280462
19	27.24227812574468	27.538924404721627	24.85493751795813	20.36385995157556
20	29.88287130062477	23.850066635089775	24.669251657051404	21.59781040723405
21	22.422241900575933	29.87257448763549	25.404100877386533	22.301082734402044
22	22.22782826484972	26.53871160391985	23.96725417339637	27.26620595783406
23	20.926654330104057	30.61786761828832	23.551949382828642	24.903528668778986
24	21.277971782809814	24.896369932129293	33.19839605073093	20.627262234329965
25	20.14252750474879	25.582677034086377	30.67611816034197	23.598677300822864
26	18.079683603453063	31.70717236766879	27.613404685344108	22.599739343534043
27	20.236816892264937	30.155001358198664	27.513770761562096	22.094410987974303
28	18.03864344882435	27.705634710262395	33.420414951756975	20.83530688915628
29	18.427029428291522	25.014538119292013	32.16444892927854	24.39398352313792
30	20.88100512585157	28.57208700708911	28.22964442653126	22.31726344052806
31	25.327316071952165	28.35913910798219	22.68569321577315	23.627851604292495
32	27.68459979229857	27.097877581680695	24.161667809122584	21.055854816898147
33	25.54345107984149	29.531161588376563	22.926687672165162	21.998699659616783
34	20.092465380643755	27.587711685313703	26.860217331399493	25.459605602643045
35	20.538317383079686	27.04305931062347	27.530932116544232	24.887691189752612
36	25.74860282054224	27.80325830388935	25.584295104698974	20.863843770869433
37	20.898460675490547	30.39629000924752	27.230510339471216	21.47473897579072
38	22.672993813086368	30.677589133626153	23.946023125661327	22.703393927626152
39	21.85297523959703	28.60959682583578	26.30271845669406	23.234709477873132
40	24.503865227466406	24.994679979955535	26.98672103383925	23.514733758738807
41	18.643654760608904	23.147480762121063	29.03058937976902	29.17827509750101
42	23.193571258358805	25.555120801229343	25.259896463093774	25.99141147731808
43	21.40447548524957	25.647448891033243	28.12118466304415	24.82689096067304
44	21.898820573620743	28.094118754615177	26.909543968862437	23.09751670290164
45	19.292403011376507	30.100183087141435	25.120154001096367	25.487259900385688
46	22.61714586073021	29.296492317106537	25.986557265480275	22.099804556682976
47	22.189975219003404	25.399589892648372	27.897890918505137	24.512543969843087
48	22.98680144704545	22.011055835203923	29.0673637118736	25.93477900587703
49	21.668417124874846	21.98992285235449	30.654151625964836	25.687508396805832
50	19.737519527170345	26.85850119590128	29.286440666331288	24.117538610597087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1075.0
1	1683.0
2	2291.0
3	6866.0
4	11441.0
5	8292.5
6	5144.0
7	5522.0
8	5900.0
9	6560.5
10	7221.0
11	7688.0
12	8155.0
13	8365.0
14	8575.0
15	8280.0
16	7985.0
17	8103.5
18	8222.0
19	8418.0
20	8614.0
21	9328.0
22	10042.0
23	9880.0
24	9718.0
25	11173.5
26	12629.0
27	14682.5
28	16736.0
29	20018.5
30	23301.0
31	27388.5
32	31476.0
33	35731.0
34	39986.0
35	46308.5
36	52631.0
37	60929.0
38	69227.0
39	89708.0
40	110189.0
41	136757.0
42	163325.0
43	188378.0
44	213431.0
45	220591.5
46	227752.0
47	208778.0
48	189804.0
49	169169.5
50	148535.0
51	134780.0
52	121025.0
53	111242.5
54	101460.0
55	94576.0
56	87692.0
57	84922.0
58	82152.0
59	78174.0
60	74196.0
61	66452.5
62	58709.0
63	49545.0
64	40381.0
65	33485.5
66	26590.0
67	23063.0
68	19536.0
69	16576.5
70	13617.0
71	11410.0
72	9203.0
73	7278.0
74	5353.0
75	4133.0
76	2913.0
77	2241.0
78	1569.0
79	1201.5
80	834.0
81	641.5
82	449.0
83	340.5
84	232.0
85	162.5
86	93.0
87	61.5
88	30.0
89	23.5
90	17.0
91	9.5
92	2.0
93	4.5
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2039466.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.59915681990313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.3044135333003	18.243189778429958
2	7.72335297944334	3.6452923627469893
3	2.794187575535612	1.9782141233786947
4	1.4519834968231002	1.3706234496575864
5	0.9533862690691213	1.124955603685228
6	0.6987835313549896	0.9894421283767258
7	0.5586740131172507	0.9228964952731034
8	0.4425653632925847	0.8355335529119287
9	0.3885209533440122	0.8251890215205251
>10	5.73751146817245	33.79927393766711
>50	1.7647525835063769	28.227913590648253
>100	0.1745432822810354	5.739489120660422
>500	0.0033485489187952714	0.529799363383597
>1k	0.0037671175336446805	1.451157350138608
>5k	2.0928430742470446E-4	0.31703012152133025
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6419	0.3147392503724014	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3525	0.1728393608915275	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3493	0.17127032272173207	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08222740658584159	0.0
2	0.0	0.0	0.0	0.3053250213536288	0.0
3	0.0	0.0	0.0	0.45987528107847836	0.0
4	0.0	0.0	0.0	0.8098688578284707	0.0
5	0.0	0.0	0.0	1.2759712591433248	0.0
6	0.0	0.0	0.0	2.063187128395374	0.0
7	0.0	0.0	0.0	2.460104752910811	0.0
8	0.0	0.0	0.0	3.6318820710911583	0.0
9	0.0	0.0	0.0	4.686618948293328	0.0
10	4.903244280610709E-5	0.0	0.0	6.241290612346565	0.0
11	4.903244280610709E-5	0.0	0.0	7.328928258671633	0.0
12	4.903244280610709E-5	0.0	0.0	8.317275208314333	0.0
13	4.903244280610709E-5	0.0	0.0	8.754448468373583	0.0
14	9.806488561221418E-5	0.0	0.0	8.934985922785671	0.0
15	9.806488561221418E-5	0.0	0.0	9.131262791338518	0.0
16	1.4709732841832127E-4	0.0	0.0	9.492533829933913	0.0
17	1.4709732841832127E-4	0.0	0.0	9.877487538404662	0.0
18	1.4709732841832127E-4	0.0	0.0	10.479458838735237	0.0
19	1.4709732841832127E-4	0.0	0.0	10.733937216898934	0.0
20	1.9612977122442836E-4	0.0	0.0	11.01861957983119	0.0
21	1.9612977122442836E-4	0.0	0.0	11.31639360499268	0.0
22	1.9612977122442836E-4	0.0	0.0	11.624268313372227	0.0
23	1.9612977122442836E-4	0.0	0.0	11.960287643922477	0.0
24	1.9612977122442836E-4	0.0	0.0	12.208440836964186	0.0
25	1.9612977122442836E-4	0.0	0.0	12.41393580476458	0.0
26	1.9612977122442836E-4	0.0	0.0	12.598297789715543	0.0
27	1.9612977122442836E-4	0.0	0.0	12.79683015063747	0.0
28	1.9612977122442836E-4	0.0	0.0	12.999334139426693	0.0
29	1.9612977122442836E-4	0.0	0.0	13.20178909577311	0.0
30	2.451622140305354E-4	0.0	0.0	13.463720405243333	0.0
31	2.451622140305354E-4	0.0	0.0	13.68971093413668	0.0
32	2.451622140305354E-4	0.0	0.0	13.892999442010801	0.0
33	2.451622140305354E-4	0.0	0.0	14.098935701796451	0.0
34	2.451622140305354E-4	7.354866420916064E-4	0.0	14.31080488716164	0.0
35	2.451622140305354E-4	7.354866420916064E-4	0.0	14.55930130730299	0.0
36	2.451622140305354E-4	7.354866420916064E-4	0.0	14.774847925878637	0.0
37	2.451622140305354E-4	7.354866420916064E-4	0.0	15.00000490324428	0.0
38	2.451622140305354E-4	7.354866420916064E-4	0.0	15.216237976019213	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	20	7.857919E-4	44.0	3
ACGGATA	35	1.4468242E-7	44.0	14
TCGCTAA	20	7.857919E-4	44.0	36
GAACGAT	40	8.3182385E-9	44.0	9
TTACGCG	25	4.444058E-5	44.0	35
TATCGTG	50	2.7284841E-11	44.0	1
TCTATCG	20	7.857919E-4	44.0	42
TATGCGA	110	0.0	42.0	2
CTATCTC	1460	0.0	40.986298	6
GAGCGAT	1920	0.0	40.906254	7
CGGTCTA	325	0.0	40.615383	31
CGACGGT	335	0.0	40.059704	28
CGTTTTT	4660	0.0	40.034336	1
CACGACC	155	0.0	39.741932	27
TCGATAG	100	0.0	39.6	1
GACGGTC	340	0.0	39.470585	29
AGACCGA	695	0.0	39.2518	8
TATCTCT	1525	0.0	39.239346	7
AGGGGAA	14650	0.0	38.954266	8
AGCGATA	470	0.0	38.851067	8
>>END_MODULE