##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549219_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2107415
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3802226898831	31.0	31.0	33.0	30.0	34.0
2	31.726832161676747	31.0	31.0	34.0	30.0	34.0
3	31.80798845979553	31.0	31.0	34.0	30.0	34.0
4	35.57015870153719	37.0	35.0	37.0	33.0	37.0
5	35.49476823501778	37.0	35.0	37.0	33.0	37.0
6	35.53393660005267	37.0	35.0	37.0	33.0	37.0
7	35.9422733538482	37.0	35.0	37.0	35.0	37.0
8	34.61538425037309	37.0	35.0	37.0	33.0	37.0
9	36.886260655827165	39.0	37.0	39.0	33.0	39.0
10	36.83114052049549	39.0	37.0	39.0	32.0	39.0
11	36.74937731770914	39.0	35.0	39.0	32.0	39.0
12	36.25664902261776	38.0	35.0	39.0	32.0	39.0
13	36.01449500928863	38.0	35.0	39.0	32.0	39.0
14	37.02880638127753	39.0	35.0	41.0	32.0	41.0
15	37.26848342637781	39.0	35.0	41.0	32.0	41.0
16	37.38338106163238	39.0	35.0	41.0	32.0	41.0
17	37.28753994823042	39.0	35.0	41.0	32.0	41.0
18	37.23743258921475	39.0	35.0	41.0	32.0	41.0
19	37.14987745650477	38.0	35.0	41.0	32.0	41.0
20	36.997022892975515	38.0	35.0	41.0	32.0	41.0
21	36.88129248391987	38.0	35.0	41.0	32.0	41.0
22	36.786728764861216	38.0	35.0	41.0	32.0	41.0
23	36.732415779521354	38.0	35.0	41.0	32.0	41.0
24	36.60185867520161	38.0	35.0	41.0	31.0	41.0
25	36.51316897715922	38.0	35.0	40.0	31.0	41.0
26	36.39822626298095	38.0	35.0	40.0	31.0	41.0
27	36.29720771656271	38.0	35.0	40.0	31.0	41.0
28	36.345715011044334	38.0	35.0	40.0	31.0	41.0
29	36.30834221071787	38.0	35.0	40.0	31.0	41.0
30	36.25978034701281	38.0	35.0	40.0	31.0	41.0
31	36.13360443956221	38.0	35.0	40.0	30.0	41.0
32	35.97016819183692	38.0	35.0	40.0	30.0	41.0
33	35.82028314309237	38.0	35.0	40.0	30.0	41.0
34	35.67937401982998	38.0	35.0	40.0	29.0	41.0
35	35.541688276870005	38.0	35.0	40.0	29.0	41.0
36	35.41529599058562	38.0	35.0	40.0	28.0	41.0
37	35.34318157553211	38.0	35.0	40.0	27.0	41.0
38	35.26816265424703	38.0	34.0	40.0	27.0	41.0
39	35.19771521034063	37.0	34.0	40.0	27.0	41.0
40	35.04723227271325	37.0	34.0	40.0	26.0	41.0
41	34.99098468977397	37.0	34.0	40.0	26.0	41.0
42	34.924014491687686	37.0	34.0	40.0	25.0	41.0
43	34.828975308612684	37.0	34.0	40.0	25.0	41.0
44	34.70123634879699	37.0	34.0	40.0	25.0	41.0
45	34.58617880199201	37.0	34.0	40.0	24.0	41.0
46	34.52399124045335	36.0	34.0	40.0	24.0	41.0
47	34.45704192102647	36.0	34.0	40.0	24.0	41.0
48	34.40761264392633	36.0	34.0	40.0	24.0	41.0
49	34.381178837580634	36.0	34.0	40.0	24.0	41.0
50	34.256247108424304	36.0	34.0	40.0	24.0	41.0
51	33.87087687996906	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	5.0
12	13.0
13	9.0
14	33.0
15	63.0
16	164.0
17	433.0
18	1007.0
19	1976.0
20	3541.0
21	5533.0
22	8323.0
23	12216.0
24	17645.0
25	24505.0
26	31853.0
27	36249.0
28	39601.0
29	44774.0
30	52048.0
31	63671.0
32	79182.0
33	102099.0
34	170478.0
35	289815.0
36	155725.0
37	193484.0
38	279698.0
39	492820.0
40	449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.037726313991314	24.8612636808602	27.563199464747097	14.537810540401392
2	31.45849298785479	26.642735294187432	28.42102765710598	13.477744060851801
3	28.745453553286847	25.854233741337136	31.71904916687031	13.681263538505705
4	25.29748530782974	28.936350932303316	30.73286467069846	15.033299089168484
5	23.591936092321635	31.745194942619275	29.64323590749805	15.019633057561041
6	20.616252612798146	40.37999160108474	27.762068695534577	11.24168709058254
7	82.98204198034085	5.357321647610936	9.137640189521285	2.522996182526935
8	79.50944640709116	7.4197535843675775	8.325792499341611	4.7450075091996595
9	76.12591729678303	6.7232604873743425	12.31390115378319	4.836921062059442
10	42.477917258821826	26.372071945962233	16.790807695684048	14.359203099531893
11	37.47297044008893	23.059293020121807	25.16912900401677	14.298607535772497
12	34.087875430325774	21.527084129134508	27.927959134769374	16.45708130577034
13	22.528690362363367	34.09883672651092	29.13446093911261	14.238011972013107
14	16.949438055627393	37.939466123188836	28.841685192522593	16.26941062866118
15	15.029028454291158	25.46356555305908	44.448672900211875	15.058733092437892
16	16.811781258081584	21.48371345938033	42.657853341653166	19.046651940884924
17	16.26067955291198	22.837931778980412	30.45702910912184	30.444359558985774
18	20.434276115525417	24.004574324468603	34.221688656481994	21.339460903523985
19	25.382138781398062	27.112125518704193	27.45733517128805	20.048400528609694
20	27.436551414885063	24.978611236989394	26.13381797130608	21.45101937681947
21	22.359335963728075	30.443837592500767	27.90788715084594	19.28893929292522
22	20.858967028326173	25.673016468042604	27.396075286547738	26.071941217083488
23	18.71828757031719	30.981463072057476	26.32224787239343	23.978001485231907
24	19.607291397280555	25.95843723234389	35.305243627856875	19.12902774251868
25	18.471492325906382	26.722880875385247	32.84825247993395	21.957374318774423
26	16.81975310985259	32.242676454329114	29.194249827395176	21.743320608423115
27	19.22217503434302	30.766507783232065	28.537995601246074	21.473321581178837
28	16.95275966053198	29.5844909521855	34.80690798917157	18.655841398110955
29	17.268027417475913	26.982915087915764	33.60035873332969	22.14869876127863
30	20.29752089645371	28.603715926858257	30.862217455982805	20.236545720705223
31	25.925838052780303	26.513999378385368	26.263123305091785	21.297039263742548
32	27.01072166611702	28.294237252748033	26.577821644052076	18.11721943708287
33	24.79188958985297	26.952166516799014	27.968625069101243	20.287318824246768
34	20.974321621512612	27.629584111340193	29.60427822711711	21.791816040030085
35	19.898169083925094	25.515382589570635	32.55542928184529	22.03101904465898
36	27.083939328513846	25.406433948700187	28.95457230777991	18.55505441500606
37	21.088679733227675	28.114728233404428	30.6857927840506	20.11079924931729
38	21.793476842482377	29.663260439922844	27.578194138316377	20.965068579278405
39	20.819961896446596	27.285655649219542	29.21835518870275	22.676027265631117
40	23.169475399956816	24.386416534000187	29.57523790995129	22.868870156091706
41	17.578502573057513	24.150677488771787	30.417359656261343	27.853460281909353
42	22.24777749043259	26.258852670214456	27.626831924419253	23.866537914933698
43	22.252854800786746	24.927600875954663	29.412289463631986	23.407254859626605
44	21.907740051200168	27.46397838109722	28.831056056827915	21.797225510874696
45	19.243196048239195	29.745398984063414	27.085789936960687	23.925615030736708
46	23.254983000500612	28.161278153567288	27.71077362550803	20.872965220424074
47	22.251383804329	25.334212767774737	29.171947623035805	23.242455804860455
48	22.22799021550098	23.356719013578246	31.3220224777749	23.09326829314587
49	22.06670257163397	21.463404217963713	32.34564620637131	24.124247004031005
50	19.77569676594311	26.175432935610687	30.599098896040882	23.449771402405318
51	19.358218480935175	28.002600342125305	27.214288595269558	25.424892581669962
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4350.0
1	4828.0
2	5306.0
3	13575.0
4	21844.0
5	15418.5
6	8993.0
7	9722.5
8	10452.0
9	11427.0
10	12402.0
11	12993.5
12	13585.0
13	13794.5
14	14004.0
15	13880.5
16	13757.0
17	13198.5
18	12640.0
19	12442.5
20	12245.0
21	11805.0
22	11365.0
23	11972.0
24	12579.0
25	13581.0
26	15444.0
27	16305.0
28	19412.5
29	22520.0
30	26996.5
31	31473.0
32	32888.5
33	34304.0
34	40082.5
35	45861.0
36	48824.5
37	51788.0
38	62030.5
39	72273.0
40	97590.5
41	122908.0
42	152690.0
43	182472.0
44	205586.5
45	228701.0
46	231123.5
47	233546.0
48	213833.0
49	194120.0
50	173916.0
51	153712.0
52	136290.0
53	118868.0
54	107277.0
55	95686.0
56	89834.5
57	83983.0
58	77132.0
59	70281.0
60	62885.0
61	55489.0
62	48117.5
63	40746.0
64	35460.0
65	30174.0
66	24560.5
67	18947.0
68	15481.0
69	12015.0
70	10895.0
71	9775.0
72	8005.5
73	6236.0
74	4854.0
75	2681.5
76	1891.0
77	1339.5
78	788.0
79	570.5
80	353.0
81	333.5
82	314.0
83	182.0
84	50.0
85	114.5
86	179.0
87	115.0
88	51.0
89	37.0
90	23.0
91	14.5
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2107415.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.869962255448606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8765985960166	19.06651393580535
2	8.258284948083867	3.9424990001100246
3	2.9676506985840914	2.125131304876738
4	1.4471664686273045	1.3817523593393841
5	0.8784265990416098	1.0484004881652655
6	0.6232613074466322	0.8926334330419595
7	0.46275049785077776	0.773208584217168
8	0.34304383738456756	0.6550754760267101
9	0.2775578717815256	0.5962766330814894
>10	2.788471258066533	15.633356553468378
>50	1.1917249659422533	20.86236583250543
>100	0.8762410448279117	29.911561462958435
>500	0.005129015317657299	0.8281124753992191
>1k	0.0034877304160069633	1.6816902664227786
>5k	0.0	0.0
>10k+	2.0516061270629195E-4	0.6014221945817747
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12281	0.582751854760453	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4336	0.20574969808983992	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3465	0.1644194427770515	No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	3259	0.15464443405783865	TruSeq Adapter, Index 13 (95% over 22bp)
GAGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	2816	0.13362342016166726	No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	2802	0.13295909918074988	TruSeq Adapter, Index 19 (95% over 21bp)
AAGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	2316	0.10989767084318941	No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	2258	0.10714548392224596	TruSeq Adapter, Index 19 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16171470735474502	0.0
2	0.0	0.0	0.0	0.5616359378670077	0.0
3	0.0	0.0	0.0	0.874151507889998	0.0
4	0.0	0.0	0.0	1.5291719950745344	0.0
5	0.0	0.0	0.0	2.503208907595324	0.0
6	0.0	0.0	0.0	3.8158597143894295	0.0
7	0.0	0.0	0.0	4.493372211927883	0.0
8	0.0	0.0	0.0	6.325711831793928	0.0
9	0.0	0.0	0.0	7.204039071563978	0.0
10	0.0	0.0	0.0	8.624262425768062	0.0
11	0.0	0.0	0.0	9.67298799714342	0.0
12	0.0	0.0	0.0	10.615754372062456	0.0
13	0.0	0.0	0.0	10.999447190040879	0.0
14	0.0	0.0	0.0	11.14284561892176	0.0
15	0.0	0.0	0.0	11.333505740445048	0.0
16	0.0	0.0	0.0	11.749133417006142	0.0
17	0.0	0.0	0.0	12.192662574765768	0.0
18	0.0	0.0	0.0	12.90324876685418	0.0
19	0.0	0.0	0.0	13.173627406087553	4.7451498636955704E-5
20	0.0	0.0	0.0	13.471243205538538	4.7451498636955704E-5
21	0.0	0.0	0.0	13.789168246406142	4.7451498636955704E-5
22	0.0	0.0	0.0	14.115065139044754	4.7451498636955704E-5
23	0.0	0.0	0.0	14.486752727868028	4.7451498636955704E-5
24	0.0	0.0	0.0	14.751769347755426	4.7451498636955704E-5
25	0.0	0.0	0.0	14.967246603065842	4.7451498636955704E-5
26	0.0	0.0	0.0	15.17005430824019	4.7451498636955704E-5
27	0.0	0.0	0.0	15.388188847474275	4.7451498636955704E-5
28	0.0	0.0	0.0	15.61538662294802	4.7451498636955704E-5
29	0.0	0.0	0.0	15.85805358697741	4.7451498636955704E-5
30	0.0	0.0	0.0	16.187224633021973	4.7451498636955704E-5
31	0.0	0.0	0.0	16.439334445280117	4.7451498636955704E-5
32	0.0	0.0	0.0	16.679439028383115	4.7451498636955704E-5
33	0.0	0.0	0.0	16.91925890249429	4.7451498636955704E-5
34	0.0	0.0	0.0	17.15708581366271	4.7451498636955704E-5
35	0.0	0.0	0.0	17.433965308209345	4.7451498636955704E-5
36	0.0	0.0	0.0	17.66818590548136	4.7451498636955704E-5
37	0.0	0.0	0.0	17.923712225641367	4.7451498636955704E-5
38	0.0	0.0	0.0	18.16742312264077	4.7451498636955704E-5
39	0.0	0.0	0.0	18.431395809558154	4.7451498636955704E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	20	7.034625E-4	45.000004	16
ATCCGAT	20	7.034625E-4	45.000004	19
TACGATA	20	7.034625E-4	45.000004	27
CGCTATA	20	7.034625E-4	45.000004	30
CCGAAAC	20	7.034625E-4	45.000004	40
TCACGAC	150	0.0	45.0	25
AATCCCG	25	3.891917E-5	45.0	36
CATCTCG	25	3.891917E-5	45.0	34
GTCGACG	30	2.1663673E-6	44.999996	1
ACGTATC	30	2.1663673E-6	44.999996	34
CTCCGAT	80	0.0	42.187504	38
TGCGAAG	230	0.0	41.08696	1
CGTTTTT	8140	0.0	40.85381	1
ACGTTAG	50	1.0822987E-9	40.5	1
TAGGGCG	860	0.0	40.2907	5
ACGTAGG	285	0.0	40.263157	2
CGTAAGG	365	0.0	40.068493	2
TCCGATT	85	0.0	39.705883	39
ATTCGGC	285	0.0	39.473686	17
AGGGCGA	2175	0.0	38.68966	6
>>END_MODULE