FastQCFastQC Report
Sat 18 Jun 2016
SRR3549214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276106
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64980.50920534814506No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59060.46281421762768926No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC17830.1397219353251219No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCC14890.1166830968587249No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73450.042.981621
TCACGAC2800.041.64285725
CCGATGA13200.040.33333218
AGACTTA9400.040.0212758
CGATGAA13550.039.9409619
GTAACGA501.3478711E-939.62
CGGTCTA2950.039.52542531
GTTTTTA42700.039.466042
GATGAAT14050.039.14590520
CACGACG3000.038.86666526
GGTCTAA3000.038.86666532
GAGCGAT7700.038.2857137
TTACTGG18900.038.0634940
ACGAGAT1800.037.8888935
ACTACCG357.2908388E-637.7142871
TACGGGA2650.037.358494
TAGCATA5050.037.02970530
TATTTTT47600.036.9747927
TCTAAAC1850.036.86486434
CGTATGG601.9826984E-1036.6666642
Produced by FastQC (version 0.11.5)