##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549214_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1276106
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.448298965759896	31.0	31.0	33.0	30.0	34.0
2	31.84347695254156	31.0	31.0	34.0	30.0	34.0
3	31.93790014309156	31.0	31.0	34.0	30.0	34.0
4	35.6443257848486	37.0	35.0	37.0	33.0	37.0
5	35.589257475476174	37.0	35.0	37.0	33.0	37.0
6	35.636956491075196	37.0	35.0	37.0	33.0	37.0
7	35.96327264349513	37.0	35.0	37.0	35.0	37.0
8	18.715990677890396	32.0	0.0	37.0	0.0	37.0
9	28.037040026455482	32.0	17.0	39.0	17.0	39.0
10	33.631909104729544	32.0	32.0	39.0	27.0	39.0
11	35.79627240997221	37.0	35.0	39.0	32.0	39.0
12	36.44146646125009	37.0	35.0	39.0	32.0	39.0
13	36.583781441353615	39.0	35.0	39.0	33.0	39.0
14	37.71977562992416	39.0	36.0	41.0	33.0	41.0
15	37.954601733711776	40.0	36.0	41.0	33.0	41.0
16	38.021906487392116	40.0	36.0	41.0	33.0	41.0
17	37.94158165544242	40.0	36.0	41.0	33.0	41.0
18	37.929512908802245	40.0	36.0	41.0	33.0	41.0
19	37.930713436031176	40.0	36.0	41.0	33.0	41.0
20	37.7440674990949	40.0	36.0	41.0	33.0	41.0
21	37.630389638478306	40.0	35.0	41.0	33.0	41.0
22	37.61155421258108	39.0	35.0	41.0	33.0	41.0
23	37.44689391006703	39.0	35.0	41.0	32.0	41.0
24	37.32701907208335	39.0	35.0	41.0	32.0	41.0
25	37.21956875055834	39.0	35.0	41.0	32.0	41.0
26	37.15943503125916	39.0	35.0	41.0	32.0	41.0
27	37.003877420841214	39.0	35.0	41.0	32.0	41.0
28	36.9874258094547	39.0	35.0	41.0	32.0	41.0
29	36.84454269472912	39.0	35.0	40.0	31.0	41.0
30	36.72847553416409	39.0	35.0	40.0	31.0	41.0
31	36.74289204815274	39.0	35.0	41.0	31.0	41.0
32	36.45750979934269	39.0	35.0	41.0	30.0	41.0
33	36.29577245150481	39.0	35.0	41.0	30.0	41.0
34	36.115150308830145	39.0	35.0	41.0	30.0	41.0
35	35.90688234362976	39.0	35.0	41.0	28.0	41.0
36	35.76717529734991	39.0	35.0	41.0	27.0	41.0
37	35.678488307397664	39.0	35.0	41.0	27.0	41.0
38	35.59066644933885	38.0	35.0	40.0	27.0	41.0
39	35.49571822403468	38.0	35.0	40.0	26.0	41.0
40	35.41965792810315	38.0	35.0	40.0	26.0	41.0
41	35.3920575563472	38.0	35.0	40.0	26.0	41.0
42	35.229940146038025	38.0	35.0	40.0	25.0	41.0
43	35.22741684468218	38.0	35.0	40.0	26.0	41.0
44	34.98123823569515	38.0	34.0	40.0	24.0	41.0
45	34.88509810313563	38.0	34.0	40.0	24.0	41.0
46	34.86095825895341	38.0	34.0	40.0	24.0	41.0
47	34.688458482289086	37.0	34.0	40.0	24.0	41.0
48	34.695069218387815	37.0	34.0	40.0	24.0	41.0
49	34.65304214540171	37.0	34.0	40.0	24.0	41.0
50	34.05254892618638	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	6.0
14	11.0
15	27.0
16	60.0
17	182.0
18	428.0
19	921.0
20	1845.0
21	3235.0
22	5202.0
23	7982.0
24	11185.0
25	15697.0
26	19919.0
27	22788.0
28	23850.0
29	25795.0
30	30432.0
31	37783.0
32	48629.0
33	64369.0
34	95877.0
35	141664.0
36	134734.0
37	199477.0
38	210260.0
39	173742.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.56210220781033	23.037427925266396	27.312699728705926	16.08777013821736
2	35.032199519475654	25.00591643640889	27.072280829335494	12.889603214779962
3	29.356338736750708	25.39350179373814	31.942722626490276	13.307436843020879
4	26.00238538177863	26.1001829001666	33.0104238989551	14.887007819099669
5	23.396097189418434	30.401157897541424	30.933637174341317	15.269107738698823
6	20.2512173753591	40.5565054940577	28.54802030552321	10.644256825059987
7	83.64908557753039	3.712700982520261	10.52365555839405	2.114557881555294
8	43.62357045574584	50.1549244341771	4.666540240387554	1.5549648696895086
9	79.39136717482717	5.056006319224265	10.649272082413216	4.903354423535349
10	42.2713316918814	29.203765204457937	15.545965617276309	12.978937486384359
11	37.396423181146396	21.15694150799385	24.878497554278407	16.56813775658135
12	33.05657993928404	19.162044532350762	28.588925998310483	19.192449530054713
13	25.42476878879968	26.984748915842417	29.26026521307791	18.330217082279997
14	18.589364833328894	31.752142847067567	30.868830645730057	18.789661673873486
15	17.823989543188418	22.336702436944893	39.600707151286805	20.238600868579883
16	20.59844558367408	20.487561378130028	37.987910095242874	20.926082942953013
17	19.91245241382769	21.929369503787303	30.2263291607437	27.931848921641304
18	22.490059603199107	23.044323904127086	31.694545750901572	22.771070741772235
19	26.218041447967487	25.708914463218573	26.341463796894615	21.73158029191932
20	28.67136429105419	24.051685361560875	26.187401360075103	21.08954898730983
21	25.069704240870273	26.682422933518062	28.32468462651222	19.92318819909945
22	23.364986921149182	21.983832064107528	29.264575199865845	25.38660581487745
23	22.90546396615955	26.865950007287797	27.60303611142021	22.625549915132442
24	22.287020043789465	24.97637343606252	32.31722129666345	20.41938522348457
25	22.716059637679002	24.888057888608	29.967573226675526	22.428309247037472
26	20.05272289292582	27.36982664449505	28.772766525664796	23.80468393691433
27	22.42305889949581	25.18662242791743	29.39434498388065	22.995973688706112
28	19.97718057904281	26.32994437766142	32.66734895063576	21.025526092660012
29	21.66356086406615	24.494987093548655	31.86710194921111	21.974350093174078
30	23.129818369320418	25.39318834015356	30.122340934060336	21.354652356465685
31	28.146799717264866	22.44241465834343	25.823716838569837	23.58706878582187
32	28.575839311154404	23.937039713001898	26.093913828475063	21.393207147368635
33	27.46550835118713	23.192352359443493	26.504067843893846	22.83807144547553
34	21.524309109117894	24.624835240959605	29.072741606104824	24.778114043817677
35	22.496563765079078	23.7307088909542	30.07602816693911	23.696699177027615
36	26.74440837986813	25.80067800010344	25.74002473148782	21.714888888540607
37	23.002007670209217	27.837812846268257	27.164671273389512	21.995508210133014
38	22.226053321589273	28.682805346891243	25.06837206313582	24.022769268383662
39	22.49719067224823	27.234649786146292	25.940086481843984	24.328073059761493
40	23.69622899665075	23.737134689438026	27.85466097643926	24.711975337471966
41	20.202083525976683	23.893626391538007	27.96993353216739	27.93435655031792
42	22.278791887194323	26.376335508178787	24.985306863222963	26.35956574140393
43	22.347673312405085	25.13552949363141	26.282926339974892	26.233870853988616
44	22.855624846211835	25.54701568678464	28.629831691097763	22.967527775905765
45	21.864798065364475	26.929424358164606	26.62349365961762	24.5822839168533
46	23.89652583719534	26.45250472923096	27.46519489760255	22.185774535971152
47	22.615362673633697	25.40635339070579	27.952301768034943	24.025982167625575
48	22.548675423514975	24.23599606929205	28.94751689906638	24.267811608126596
49	22.74380027991405	23.411613141854986	29.843523970579245	24.001062607651715
50	20.693422019800863	25.80796579594485	29.34458422732908	24.15402795692521
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	414.0
1	976.0
2	1538.0
3	10997.0
4	20456.0
5	13967.0
6	7478.0
7	7449.0
8	7420.0
9	7409.0
10	7398.0
11	7367.0
12	7336.0
13	6894.0
14	6452.0
15	6069.0
16	5686.0
17	5345.5
18	5005.0
19	4954.0
20	4903.0
21	4990.0
22	5077.0
23	5578.5
24	6080.0
25	7117.5
26	8155.0
27	9882.0
28	11609.0
29	13328.5
30	15048.0
31	17234.0
32	19420.0
33	21764.0
34	24108.0
35	27296.0
36	30484.0
37	33681.0
38	36878.0
39	45432.5
40	53987.0
41	63866.5
42	73746.0
43	79564.5
44	85383.0
45	92183.0
46	98983.0
47	101409.5
48	103836.0
49	103036.0
50	102236.0
51	93958.5
52	85681.0
53	80746.5
54	75812.0
55	74135.5
56	72459.0
57	70742.0
58	69025.0
59	65039.0
60	61053.0
61	56052.5
62	51052.0
63	44164.0
64	37276.0
65	31262.5
66	25249.0
67	21724.5
68	18200.0
69	15544.0
70	12888.0
71	10716.0
72	8544.0
73	6721.5
74	4899.0
75	3658.0
76	2417.0
77	1867.0
78	1317.0
79	1001.5
80	686.0
81	481.5
82	277.0
83	179.0
84	81.0
85	63.0
86	45.0
87	31.0
88	17.0
89	11.0
90	5.0
91	5.0
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1276106.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.99512660479855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.71190902068265	23.698651730203046
2	7.282699378698357	4.951525748070766
3	4.005627972495677	4.085154901701392
4	2.8246722924564467	3.8410036879649376
5	2.2932987534406912	3.8980490732921496
6	1.9413872172396391	3.9598622543399435
7	1.656858067330282	3.942756983555234
8	1.471025477090569	4.000615786606247
9	1.2852719710068827	3.932368503838015
>10	7.44388147939736	38.652722208126875
>50	0.05179697425426598	1.169874603105798
>100	0.028321400033466747	1.9218698231302265
>500	0.0013928553744721904	0.33429308126933477
>1k	0.0013928553744721904	0.632363187018692
>5k	4.642851248240634E-4	0.9788884277773438
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6498	0.50920534814506	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5906	0.46281421762768926	No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1783	0.1397219353251219	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCC	1489	0.1166830968587249	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.836339614420745E-5	0.0	0.0	0.026016647519876875	0.0
2	7.836339614420745E-5	0.0	0.0	0.09630861386123096	0.0
3	7.836339614420745E-5	0.0	0.0	0.1566484288922707	0.0
4	7.836339614420745E-5	0.0	0.0	0.27576079103146606	0.0
5	7.836339614420745E-5	0.0	0.0	0.5603766458272276	0.0
6	7.836339614420745E-5	0.0	0.0	0.9019626896198278	0.0
7	7.836339614420745E-5	0.0	0.0	1.130940533153202	0.0
8	7.836339614420745E-5	0.0	0.0	1.6855182876657582	0.0
9	7.836339614420745E-5	0.0	0.0	2.206634872024738	0.0
10	7.836339614420745E-5	0.0	0.0	2.946698785210633	0.0
11	7.836339614420745E-5	0.0	0.0	3.371350028916093	0.0
12	1.567267922884149E-4	0.0	0.0	3.8097148669467895	0.0
13	1.567267922884149E-4	0.0	0.0	4.0000595561810695	0.0
14	2.3509018843262237E-4	0.0	0.0	4.091039459104494	0.0
15	2.3509018843262237E-4	0.0	0.0	4.186250985419706	0.0
16	2.3509018843262237E-4	0.0	0.0	4.33568998186671	0.0
17	2.3509018843262237E-4	0.0	0.0	4.496099853773903	0.0
18	2.3509018843262237E-4	0.0	0.0	4.741063830120695	0.0
19	2.3509018843262237E-4	0.0	0.0	4.902570789573907	0.0
20	2.3509018843262237E-4	0.0	0.0	5.0470728920638255	0.0
21	2.3509018843262237E-4	0.0	0.0	5.198549336810578	0.0
22	2.3509018843262237E-4	0.0	0.0	5.372437712854575	0.0
23	2.3509018843262237E-4	0.0	0.0	5.5487553541790415	0.0
24	2.3509018843262237E-4	0.0	0.0	5.68933928686175	0.0
25	2.3509018843262237E-4	0.0	0.0	5.801869123724831	0.0
26	2.3509018843262237E-4	0.0	0.0	5.913693690022615	0.0
27	2.3509018843262237E-4	0.0	0.0	6.050202726105825	0.0
28	2.3509018843262237E-4	0.0	0.0	6.180207600309065	0.0
29	2.3509018843262237E-4	0.0	0.0	6.312406649604343	0.0
30	2.3509018843262237E-4	0.0	0.0	6.49115355620928	0.0
31	2.3509018843262237E-4	0.0	0.0	6.635107114926189	0.0
32	2.3509018843262237E-4	0.0	0.0	6.782900480054165	0.0
33	2.3509018843262237E-4	0.0	0.0	6.936414373100667	0.0
34	2.3509018843262237E-4	0.0	0.0	7.093219528785227	0.0
35	2.3509018843262237E-4	0.0	0.0	7.275179334632076	0.0
36	3.134535845768298E-4	0.0	0.0	7.43637284050071	0.0
37	3.134535845768298E-4	0.0	0.0	7.607910314660381	0.0
38	3.134535845768298E-4	0.0	0.0	7.815024770669521	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7345	0.0	42.98162	1
TCACGAC	280	0.0	41.642857	25
CCGATGA	1320	0.0	40.333332	18
AGACTTA	940	0.0	40.021275	8
CGATGAA	1355	0.0	39.94096	19
GTAACGA	50	1.3478711E-9	39.6	2
CGGTCTA	295	0.0	39.525425	31
GTTTTTA	4270	0.0	39.46604	2
GATGAAT	1405	0.0	39.145905	20
CACGACG	300	0.0	38.866665	26
GGTCTAA	300	0.0	38.866665	32
GAGCGAT	770	0.0	38.285713	7
TTACTGG	1890	0.0	38.06349	40
ACGAGAT	180	0.0	37.888893	5
ACTACCG	35	7.2908388E-6	37.714287	1
TACGGGA	265	0.0	37.35849	4
TAGCATA	505	0.0	37.029705	30
TATTTTT	4760	0.0	36.974792	7
TCTAAAC	185	0.0	36.864864	34
CGTATGG	60	1.9826984E-10	36.666664	2
>>END_MODULE