FastQCFastQC Report
Sat 18 Jun 2016
SRR3549213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768255
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26600.3462392044308205No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24610.3203363466557328No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10550.1373241957422991No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.12808247261651404No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGT207.854849E-444.024
GTTGAGC207.854849E-444.019
CGTGGTA207.854849E-444.014
TGTCAAA207.854849E-444.035
AAGTACG254.441457E-544.01
ATCTCGC408.305506E-944.044
CTCACGA207.854849E-444.024
CAAACGA207.854849E-444.036
ACACGAG207.854849E-444.02
ACTGCGA207.854849E-444.028
GTTCTCG207.854849E-444.01
ACGCCCT207.854849E-444.039
CGAGTGA207.854849E-444.021
CTCCGCG207.854849E-444.033
CGACGTC207.854849E-444.018
TTTACGA254.441457E-544.013
CACGGCT207.854849E-444.013
ACGCATC254.441457E-544.019
CGACGAT254.441457E-544.036
CGTGCGT254.441457E-544.035
Produced by FastQC (version 0.11.5)