##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549212_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	298468
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.046299770829705	31.0	31.0	33.0	30.0	34.0
2	31.19033531232829	31.0	31.0	33.0	30.0	34.0
3	31.123594489191472	31.0	31.0	34.0	28.0	34.0
4	35.02007250358498	35.0	35.0	37.0	32.0	37.0
5	32.75029818942064	35.0	35.0	37.0	28.0	37.0
6	33.955764102014285	35.0	35.0	37.0	28.0	37.0
7	15.440697160164573	0.0	0.0	35.0	0.0	37.0
8	25.00990055885388	17.0	17.0	35.0	17.0	37.0
9	32.84935403460337	32.0	32.0	37.0	27.0	39.0
10	35.70004154549231	37.0	35.0	37.0	32.0	39.0
11	36.58503759196966	37.0	35.0	39.0	33.0	39.0
12	36.08591205757401	37.0	35.0	39.0	32.0	39.0
13	35.351106316255006	37.0	35.0	39.0	30.0	39.0
14	36.30983220981814	38.0	35.0	40.0	30.0	41.0
15	36.91997802109439	39.0	35.0	40.0	31.0	41.0
16	37.12141670128791	39.0	35.0	41.0	32.0	41.0
17	37.1386982859134	39.0	35.0	41.0	32.0	41.0
18	36.971022019110926	38.0	35.0	40.0	32.0	41.0
19	36.86979508691049	38.0	35.0	40.0	31.0	41.0
20	36.94645322111583	38.0	35.0	40.0	32.0	41.0
21	36.74285685567632	38.0	35.0	40.0	31.0	41.0
22	36.79131766219494	38.0	35.0	40.0	32.0	41.0
23	36.81727019311953	38.0	35.0	40.0	32.0	41.0
24	36.63406462334321	38.0	35.0	40.0	32.0	41.0
25	36.3914322473431	38.0	35.0	40.0	31.0	41.0
26	36.218174142621656	38.0	34.0	40.0	31.0	41.0
27	36.08449817065816	38.0	34.0	40.0	31.0	41.0
28	36.060371631129634	38.0	34.0	40.0	31.0	41.0
29	36.19643311845826	38.0	35.0	40.0	31.0	41.0
30	35.96171113821247	37.0	34.0	40.0	30.0	41.0
31	35.76968050176233	37.0	34.0	40.0	30.0	41.0
32	35.54716083466234	37.0	34.0	40.0	29.0	41.0
33	35.32684575900934	37.0	34.0	40.0	29.0	41.0
34	35.16774662610397	37.0	34.0	40.0	27.0	41.0
35	34.95558652853907	37.0	34.0	40.0	26.0	41.0
36	34.716294544138734	37.0	34.0	40.0	24.0	41.0
37	34.51283219641637	37.0	33.0	40.0	24.0	41.0
38	34.50428856694855	37.0	33.0	40.0	24.0	41.0
39	34.4449354704692	37.0	33.0	40.0	23.0	41.0
40	34.25090797003364	36.0	33.0	40.0	23.0	41.0
41	34.177895117734565	36.0	33.0	40.0	23.0	41.0
42	34.145003819504936	36.0	33.0	40.0	23.0	41.0
43	34.002465925995416	36.0	33.0	40.0	23.0	41.0
44	33.75440918289398	36.0	33.0	40.0	23.0	41.0
45	33.67633046088693	35.0	33.0	40.0	23.0	41.0
46	33.699043113499606	35.0	33.0	40.0	23.0	41.0
47	33.68984949810365	35.0	33.0	40.0	23.0	41.0
48	33.72516651701355	35.0	33.0	40.0	23.0	41.0
49	33.706584960531785	36.0	33.0	40.0	22.0	41.0
50	33.51478550464371	35.0	33.0	40.0	22.0	41.0
51	33.293636168701504	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	4.0
15	11.0
16	52.0
17	102.0
18	213.0
19	422.0
20	802.0
21	1145.0
22	1790.0
23	2622.0
24	3932.0
25	5025.0
26	5745.0
27	6182.0
28	6597.0
29	7696.0
30	8990.0
31	11892.0
32	16172.0
33	25917.0
34	32440.0
35	30567.0
36	30135.0
37	47671.0
38	45044.0
39	7298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.36941983730249	23.851468164091294	29.601163273784792	16.177948724821423
2	29.86015251216211	24.33862256590321	32.08853210394414	13.712692817990538
3	29.468150689521156	21.630459546752082	35.0044225846657	13.896967179061074
4	25.615141321682728	27.291367918838873	32.008791562244525	15.084699197233874
5	21.126217886004532	36.1499390219387	30.011257488239945	12.712585603816825
6	21.401624294731764	38.85944221826125	29.10697294182291	10.631960545184073
7	37.588619215460284	56.76990498143854	4.787447900612461	0.854027902488709
8	87.42411246766822	2.9919455351997533	8.01358939651822	1.5703526006138013
9	83.9681305868636	4.097591701622955	9.687135639331519	2.2471420721819424
10	64.05845852821743	15.969551174665291	12.504523097953548	7.46746719916373
11	61.12112521275313	15.741050966937827	15.545050055617352	7.5927737646916915
12	50.95152579170966	21.510513689909804	18.63985418872375	8.89810632965678
13	19.160177975528363	52.09201656458984	19.407440663655734	9.34036479622606
14	11.36805285658764	52.894782690271654	26.55393543026388	9.183229022876823
15	10.177305439779138	20.441052307115	60.19774314164332	9.183899111462535
16	10.894970315075653	14.431027781872762	57.32507337470013	17.348928528351447
17	12.729672862752455	14.354637683101707	30.083627055496738	42.8320623986491
18	21.726617258801614	21.14933594221156	37.38558237398984	19.738464424996984
19	31.704571344331722	22.066352171757107	26.316389026629324	19.91268745728185
20	35.26106651299302	21.09840920969752	24.425734082045647	19.214790195263813
21	21.249849230068214	28.592009863703982	28.495517107361596	21.66262379886621
22	21.123537531661686	24.378157792460165	25.92304702681695	28.575257649061204
23	17.46853934090087	31.99874023345886	24.966495570714446	25.566224854925824
24	19.550839620997895	20.912124582869858	44.597075733411955	14.939960062720292
25	13.966321347682165	23.297639948001127	41.84971253199673	20.88632617231998
26	13.348499671656594	38.859777262554104	29.405162362464317	18.38656070332498
27	15.71123202487369	37.62982966348151	31.14906790677728	15.509870404867524
28	11.53322969296541	29.67688328397014	45.5502767465859	13.23961027647855
29	12.301486256483107	23.826004797834273	44.033531232828985	19.83897771285364
30	17.083908492702733	30.899794952892773	34.840920969752204	17.17537558465229
31	33.615999035072434	24.988273449750057	24.655909511237386	16.739818003940123
32	34.21338300923382	25.895238350509935	26.72045244381307	13.170926196443169
33	31.086414624013294	27.11211922216117	24.386198855488693	17.41526729833684
34	18.63080799281665	27.81973276867202	29.078829221223046	24.470630017288286
35	19.37929694305587	26.42393824463594	33.01325435222536	21.18351046008282
36	34.50822198694667	22.97968291408124	26.520431000978327	15.991664097993755
37	19.763592746961148	34.51492287280378	29.658455847863085	16.063028532371977
38	19.691893268290066	35.08382808207245	23.10465443531635	22.11962421432113
39	20.121085007437983	33.32585067745956	27.731951163943876	18.821113151158585
40	25.15311524183497	25.505916882211828	24.015639867590497	25.325328008362707
41	15.7970033638447	22.702266239596874	27.54399131565193	33.956739080906495
42	23.917806934076687	24.088679523432997	22.804119704624952	29.189393837865367
43	23.857834005655548	25.17154267794202	24.67132154870874	26.299301767693688
44	18.727635793451896	33.00454320061112	28.691853062974925	19.575967942962063
45	15.706541404773711	42.49400270715788	20.680608976506694	21.118846911561707
46	22.263023171663292	35.00475762895855	25.077395231649625	17.654823967728532
47	22.21712210354209	27.642159293458597	26.425613466100216	23.715105136899098
48	25.404398461476607	21.36510446681051	32.625273061098675	20.605224010614204
49	21.161732581047215	21.3945883645818	33.79189728882158	23.65178176554941
50	17.759357787099457	36.82672849350684	25.42818660626935	19.985727113124355
51	17.14053097819532	38.97704276505354	21.833496388222525	22.048929868528617
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	172.0
1	256.0
2	340.0
3	1401.0
4	2462.0
5	1956.0
6	1450.0
7	1611.5
8	1773.0
9	1908.0
10	2043.0
11	2051.5
12	2060.0
13	1987.0
14	1914.0
15	1828.5
16	1743.0
17	1618.5
18	1494.0
19	1444.0
20	1394.0
21	1331.0
22	1268.0
23	1331.0
24	1394.0
25	1622.0
26	2045.0
27	2240.0
28	2493.0
29	2746.0
30	3053.5
31	3361.0
32	3910.5
33	4460.0
34	4957.0
35	5454.0
36	7031.0
37	8608.0
38	14060.5
39	19513.0
40	25965.5
41	32418.0
42	35507.0
43	38596.0
44	36811.5
45	35027.0
46	31482.0
47	27937.0
48	25347.0
49	22757.0
50	20475.0
51	18193.0
52	16291.5
53	14390.0
54	12726.0
55	11062.0
56	9759.5
57	8457.0
58	7622.5
59	6788.0
60	6102.5
61	5417.0
62	4553.0
63	3689.0
64	2935.5
65	2182.0
66	1823.5
67	1465.0
68	1170.0
69	875.0
70	733.5
71	592.0
72	501.0
73	410.0
74	316.5
75	167.0
76	111.0
77	94.5
78	78.0
79	56.0
80	34.0
81	21.0
82	8.0
83	7.0
84	6.0
85	8.5
86	11.0
87	6.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	298468.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.024123189085596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.24907282196843	18.3299382178324
2	5.239057960342219	2.6220566358872643
3	2.6496538981643885	1.9891579666831953
4	1.7981228828877076	1.7998579412198294
5	1.3362074736574328	1.6718710213490224
6	1.0791415067814538	1.6202742002492732
7	1.0630748838517052	1.8621761796909553
8	0.9452529823668813	1.8923301660479515
9	0.8341255071027863	1.8785933500408754
>10	11.550563001245164	61.26117372716673
>50	0.23430491772550174	3.767238028867416
>100	0.017405508173894413	0.5337255585188362
>500	0.004016655732437173	0.7716070064462522
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	993	0.33269898280552684	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	699	0.23419596070600535	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	611	0.20471206293471997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01708725893563129	0.0
2	0.0	0.0	0.0	0.07169947867108031	0.0
3	0.0	0.0	0.0	0.08912178189956713	0.0
4	0.0	0.0	0.0	0.12731683128509588	0.0
5	0.0	0.0	0.0	0.18963506975622177	0.0
6	0.0	0.0	0.0	0.25898923837731347	0.0
7	0.0	0.0	0.0	0.29751933205569775	0.0
8	0.0	0.0	0.0	0.5595239690687109	0.0
9	0.0	0.0	0.0	0.6426149536968787	0.0
10	0.0	0.0	0.0	0.7803181580604956	0.0
11	0.0	0.0	0.0	0.9458300387311203	0.0
12	0.0	0.0	0.0	1.0768323572376268	0.0
13	0.0	0.0	0.0	1.1381454628301861	0.0
14	0.0	0.0	0.0	1.1582481204015171	0.0
15	0.0	0.0	0.0	1.201803878472734	0.0
16	0.0	0.0	0.0	1.2946111475937119	0.0
17	0.0	0.0	0.0	1.4102014286288647	0.0
18	0.0	0.0	0.0	1.5991664097993754	0.0
19	0.0	0.0	0.0	1.6604795153919347	0.0
20	0.0	0.0	0.0	1.7231327981559161	0.0
21	0.0	0.0	0.0	1.8186204216197381	0.0
22	0.0	0.0	0.0	1.902381494833617	0.0
23	0.0	0.0	0.0	2.0008845169331386	0.0
24	0.0	0.0	0.0	2.0796199257541845	0.0
25	0.0	0.0	0.0	2.1412680756395996	0.0
26	0.0	0.0	0.0	2.2119624214321134	0.0
27	0.0	0.0	0.0	2.275955881367517	0.0
28	0.0	0.0	0.0	2.3342535883243767	0.0
29	0.0	0.0	0.0	2.3959017382097914	0.0
30	0.0	0.0	0.0	2.499765468995001	0.0
31	0.0	0.0	0.0	2.5935778709945456	0.0
32	0.0	0.0	0.0	2.668627792594181	0.0
33	0.0	0.0	0.0	2.7523888658080597	0.0
34	0.0	0.0	0.0	2.847206400686171	0.0
35	0.0	0.0	0.0	2.979883940656955	0.0
36	0.0	0.0	0.0	3.073361298363644	0.0
37	0.0	0.0	0.0	3.1715292761703098	0.0
38	0.0	0.0	0.0	3.254955305091333	0.0
39	0.0	0.0	0.0	3.350442928555155	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	7.0248847E-4	45.000004	6
CGTGGAT	20	7.0248847E-4	45.000004	27
AGACTAC	20	7.0248847E-4	45.000004	20
CTCACTC	20	7.0248847E-4	45.000004	13
ACTATAG	25	3.8838425E-5	45.000004	2
TCGTTAA	20	7.0248847E-4	45.000004	1
ACGCCTG	20	7.0248847E-4	45.000004	39
TTGGACG	20	7.0248847E-4	45.000004	16
GATCGAG	20	7.0248847E-4	45.000004	1
ATAGGCG	40	6.7866495E-9	45.000004	5
ACGTTGG	20	7.0248847E-4	45.000004	2
ACTTGCG	20	7.0248847E-4	45.000004	1
TAGACGG	25	3.8838425E-5	45.000004	2
GACACGA	25	3.8838425E-5	45.000004	25
TAGGTTA	20	7.0248847E-4	45.000004	6
GCTATTC	25	3.8838425E-5	45.000004	39
CATTGAG	20	7.0248847E-4	45.000004	2
GCTATGC	20	7.0248847E-4	45.000004	45
ACATTAG	20	7.0248847E-4	45.000004	2
CATCTAA	20	7.0248847E-4	45.000004	20
>>END_MODULE