##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549211_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1003897
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62511891160149	31.0	31.0	33.0	30.0	34.0
2	31.8205861756734	31.0	31.0	34.0	30.0	34.0
3	31.859662893703238	31.0	31.0	34.0	30.0	34.0
4	35.74872123335362	37.0	35.0	37.0	35.0	37.0
5	27.15745440020241	35.0	26.0	37.0	0.0	37.0
6	31.390452406970038	36.0	27.0	37.0	17.0	37.0
7	14.88831125105464	0.0	0.0	32.0	0.0	37.0
8	25.064721779226353	17.0	17.0	35.0	17.0	37.0
9	33.291799855961315	32.0	32.0	37.0	28.0	39.0
10	36.224001067838635	37.0	35.0	39.0	33.0	39.0
11	37.16709682367813	39.0	37.0	39.0	34.0	39.0
12	36.92652831914031	39.0	35.0	39.0	33.0	39.0
13	36.25808026122202	39.0	35.0	39.0	32.0	39.0
14	37.53423408975224	40.0	35.0	41.0	33.0	41.0
15	37.888424808521194	40.0	35.0	41.0	33.0	41.0
16	38.02376140181712	40.0	35.0	41.0	33.0	41.0
17	38.06948521611281	40.0	35.0	41.0	34.0	41.0
18	38.04804576565126	40.0	36.0	41.0	34.0	41.0
19	37.968457919487754	40.0	36.0	41.0	34.0	41.0
20	37.963451429778154	40.0	35.0	41.0	34.0	41.0
21	37.80404164969115	40.0	35.0	41.0	34.0	41.0
22	37.709723208655866	39.0	35.0	41.0	33.0	41.0
23	37.633774182012694	39.0	35.0	41.0	33.0	41.0
24	37.48580780697621	39.0	35.0	41.0	33.0	41.0
25	37.314057119405675	39.0	35.0	41.0	33.0	41.0
26	37.0777689344624	39.0	35.0	40.0	33.0	41.0
27	36.86984222484976	38.0	35.0	40.0	33.0	41.0
28	36.921256862008754	38.0	35.0	40.0	33.0	41.0
29	36.97103587320213	38.0	35.0	40.0	33.0	41.0
30	36.7971495083659	38.0	35.0	40.0	32.0	41.0
31	36.59450023259358	38.0	35.0	40.0	32.0	41.0
32	36.29701254212335	38.0	35.0	40.0	31.0	41.0
33	36.109878802307406	38.0	35.0	40.0	31.0	41.0
34	35.906272257014415	38.0	35.0	40.0	30.0	41.0
35	35.77838861954962	38.0	35.0	40.0	30.0	41.0
36	35.56608397076592	38.0	35.0	40.0	30.0	41.0
37	35.326325310265894	38.0	34.0	40.0	29.0	41.0
38	35.186772148935596	38.0	34.0	40.0	29.0	41.0
39	35.155870572379435	38.0	34.0	40.0	28.0	41.0
40	34.90897372937662	37.0	34.0	40.0	27.0	41.0
41	34.803326436875494	37.0	34.0	40.0	27.0	40.0
42	34.810676792539475	37.0	34.0	40.0	27.0	41.0
43	34.67641003011265	37.0	34.0	40.0	27.0	41.0
44	34.42571897316159	36.0	34.0	40.0	26.0	40.0
45	34.22264435494876	36.0	33.0	39.0	25.0	40.0
46	34.222753927942804	36.0	34.0	39.0	25.0	40.0
47	34.07889952853729	36.0	33.0	39.0	25.0	40.0
48	34.07535135576658	36.0	33.0	39.0	25.0	40.0
49	33.959143218876044	36.0	33.0	39.0	24.0	40.0
50	34.02646685865184	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	1.0
14	6.0
15	20.0
16	42.0
17	103.0
18	279.0
19	653.0
20	1125.0
21	2019.0
22	3282.0
23	5242.0
24	8104.0
25	11978.0
26	15378.0
27	17009.0
28	17657.0
29	19344.0
30	24268.0
31	33299.0
32	47560.0
33	98474.0
34	91671.0
35	106099.0
36	122996.0
37	238873.0
38	113843.0
39	24569.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.77586047174162	23.82186618746744	30.853364438782066	16.548908902008872
2	30.97907454649232	25.871578458746264	30.300917325183757	12.848429669577655
3	27.654928742689737	23.945882894360675	34.820902941237996	13.57828542171159
4	27.071701578946843	26.352504290778832	32.07052117896557	14.50527295130875
5	17.313429564985253	48.22845371586926	23.46884192302597	10.989274796119522
6	21.5738267969722	38.85727320631499	28.78900923102669	10.779890765686122
7	38.33640303736339	56.53617851233742	4.089961420344916	1.0374570299542683
8	87.75392296221625	3.2633825980155335	7.471483628300513	1.5112108114677103
9	83.9059186350791	4.638523673245363	8.892047690151479	2.563510001524061
10	59.147203348550704	18.744154031738315	12.951527895790104	9.15711472392088
11	55.27758325804341	16.182935101907862	18.305463608318384	10.234018031730347
12	48.337827486285946	19.928040426458093	20.904933474250846	10.829198613005119
13	20.35447859690785	45.66374837259201	21.540058392444646	12.441714638055497
14	13.393107061780244	47.20882720040004	26.642573889552413	12.755491848267303
15	11.729988235844912	21.4755099377725	54.60689692269227	12.18760490369032
16	13.552485962205285	14.970460116924345	52.61844591626432	18.85860800460605
17	14.42528466565793	16.425091418741168	29.714402971619595	39.4352209439813
18	21.22279476878604	22.266029283880716	34.90786405378241	21.603311893550835
19	30.85366327422036	21.507086882419213	26.34682641745119	21.29242342590923
20	33.27054468735338	21.03632145528874	24.880739757166324	20.812394100191554
21	22.086927244528074	27.47502980883497	28.018810694722667	22.419232251914288
22	22.642263100696585	24.537477450375885	25.70323449517231	27.117024953755216
23	20.39063768494178	30.4112872137281	24.58200393068213	24.616071170647984
24	20.378584655597137	21.628115234929478	41.25204079701404	16.74125931245935
25	15.896351916581084	23.811207723501514	38.86713477577879	21.425305584138613
26	15.519121981637559	35.716612361626744	28.57305082095076	20.191214835784947
27	17.330064737717116	34.39057991009038	30.42055111231531	17.8588042398772
28	13.617532475941257	28.321032934653655	41.99942822819472	16.06200636121036
29	14.109415607378049	23.46924037027703	40.80657677032604	21.614767252018883
30	16.950344507454453	29.62584807007093	33.63512392207567	19.788683500398946
31	30.324226489370922	25.766886443529565	24.79895845888572	19.109928608213792
32	31.706041556056046	25.548935797198318	26.37123131157878	16.373791335166853
33	28.600244845835775	26.97975987576415	25.882635369963253	18.53735990843682
34	19.102258498630835	26.780735473858375	29.672665622070788	24.444340405440002
35	20.38426252892478	24.62324322116711	31.990632505127518	23.00186174478059
36	31.453824446133417	23.365146025936923	26.56178870939947	18.619240818530187
37	21.409268082283344	31.535705356226785	29.68501748685373	17.37000907463614
38	20.81956615071068	33.26008544701299	23.468144640336607	22.45220376193972
39	20.85682096868503	31.860738701281104	26.67205898613105	20.610381343902812
40	23.564170427842697	25.92387466044823	24.934330912434245	25.577623999274824
41	16.599412091081057	23.819276280335533	27.41615922749047	32.16515240109294
42	21.91609298563498	25.84677511736762	23.518647829408796	28.718484067588605
43	23.72932681340815	27.068812836376637	24.263644577083106	24.938215773132104
44	20.5883671332816	31.443464817605793	27.02578053326188	20.94238751585073
45	16.28165040835863	38.859763501634134	21.65172323455494	23.206862855452304
46	21.564961345636053	34.080089889699835	25.149392816195288	19.205555948468817
47	23.042304140763445	26.22201281605583	27.936830172816535	22.798852870364193
48	24.715284536162574	21.409865753159934	31.169432720687478	22.70541698999001
49	19.929534603649575	22.40090367836541	33.66032571070538	24.00923600727963
50	18.168895813016675	33.69548868061166	26.60531907157806	21.53029643479361
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	356.0
1	697.0
2	1038.0
3	5799.0
4	10560.0
5	7863.0
6	5166.0
7	5554.5
8	5943.0
9	6342.0
10	6741.0
11	6806.5
12	6872.0
13	6358.5
14	5845.0
15	5523.5
16	5202.0
17	4759.0
18	4316.0
19	4198.0
20	4080.0
21	4067.5
22	4055.0
23	4316.5
24	4578.0
25	5559.5
26	6541.0
27	8239.5
28	9938.0
29	11293.0
30	12648.0
31	14725.5
32	16803.0
33	17965.0
34	19127.0
35	21005.5
36	22884.0
37	28036.0
38	33188.0
39	51808.0
40	70428.0
41	89351.0
42	108274.0
43	110072.5
44	111871.0
45	104380.5
46	96890.0
47	89712.5
48	82535.0
49	77823.0
50	73111.0
51	67810.0
52	62509.0
53	54708.5
54	46908.0
55	42352.5
56	37797.0
57	35193.0
58	32589.0
59	30632.5
60	28676.0
61	25663.5
62	22651.0
63	19205.5
64	15760.0
65	13294.5
66	10829.0
67	8878.0
68	6927.0
69	5668.5
70	4410.0
71	3543.5
72	2677.0
73	2145.0
74	1613.0
75	1236.0
76	859.0
77	609.5
78	360.0
79	265.0
80	170.0
81	143.0
82	116.0
83	77.0
84	38.0
85	26.0
86	14.0
87	8.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1003897.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.24774206031918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.90840341240073	13.66909223619822
2	5.531557569810637	2.01876871451421
3	2.0891985055442492	1.1436946632592733
4	1.397723143217478	1.020211655566844
5	0.9757404579881843	0.8902530097593045
6	0.7937390252583779	0.869036699767443
7	0.7119201095289661	0.9093654168367126
8	0.5985046702587694	0.8737087075822727
9	0.537964017341547	0.8834965763563463
>10	10.02030757077187	43.91906313763813
>50	2.206332245035621	27.081595973941074
>100	0.2241157244829953	5.3797279838174115
>500	0.0022467741802806545	0.271820424354293
>1k	0.0022467741802806545	1.0701648004084363
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3904	0.38888451703710636	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3562	0.3548172770712533	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1556	0.1549959806633549	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1419	0.14134916231446054	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0036856370723291334	0.0
2	0.0	0.0	0.0	0.02121731611908393	0.0
3	0.0	0.0	0.0	0.0331707336509622	0.0
4	0.0	0.0	0.0	0.05558339152323396	0.0
5	0.0	0.0	0.0	0.09692229382097964	0.0
6	0.0	0.0	0.0	0.16495716193992013	0.0
7	0.0	0.0	0.0	0.20998170130999494	0.0
8	0.0	0.0	0.0	0.4258404995731634	0.0
9	0.0	0.0	0.0	0.5209697807643613	0.0
10	0.0	0.0	0.0	0.6896125797766105	0.0
11	0.0	0.0	0.0	0.8631363576143768	0.0
12	0.0	0.0	0.0	1.0125540767628551	0.0
13	0.0	0.0	0.0	1.0749110715541534	0.0
14	0.0	0.0	0.0	1.0975229530519566	0.0
15	0.0	0.0	0.0	1.137467289970983	0.0
16	0.0	0.0	0.0	1.225723356081351	0.0
17	0.0	0.0	0.0	1.3164697175108602	0.0
18	0.0	0.0	0.0	1.4495510993657716	0.0
19	0.0	0.0	0.0	1.5029430310081613	0.0
20	0.0	0.0	0.0	1.5654992494249909	0.0
21	0.0	0.0	0.0	1.654253374599187	0.0
22	0.0	0.0	0.0	1.7326478712457554	0.0
23	0.0	0.0	0.0	1.8288728823773754	0.0
24	0.0	0.0	0.0	1.9044782482665055	0.0
25	0.0	0.0	0.0	1.9625519351088807	0.0
26	0.0	0.0	0.0	2.0222194109555063	0.0
27	0.0	0.0	0.0	2.0766074607255525	0.0
28	0.0	0.0	0.0	2.1364741601977095	0.0
29	0.0	0.0	0.0	2.1970381423592262	0.0
30	0.0	0.0	0.0	2.279915170580249	0.0
31	0.0	0.0	0.0	2.362692586988506	0.0
32	0.0	0.0	0.0	2.43819834106487	0.0
33	0.0	0.0	0.0	2.5127079770135783	0.0
34	0.0	0.0	0.0	2.5885125665282396	0.0
35	0.0	0.0	0.0	2.698085560570457	0.0
36	0.0	0.0	0.0	2.7834528841106208	0.0
37	0.0	0.0	0.0	2.872804680161411	0.0
38	0.0	0.0	0.0	2.9496053878037287	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAA	30	2.5273948E-6	44.000004	1
CGGCCTC	30	2.5273948E-6	44.000004	19
GGTCCGT	30	2.5273948E-6	44.000004	9
CCCGTGC	30	2.5273948E-6	44.000004	39
AACGACC	30	2.5273948E-6	44.000004	27
GTCCAAC	30	2.5273948E-6	44.000004	14
ATACCGG	30	2.5273948E-6	44.000004	15
CGGGTAC	25	4.4424356E-5	44.0	6
AGCGTAA	35	1.4458419E-7	44.0	1
GTACCGA	25	4.4424356E-5	44.0	27
ACGCCGG	70	0.0	44.0	27
AATCATA	40	8.310963E-9	44.0	32
ATCTACG	25	4.4424356E-5	44.0	1
GTCGACA	20	7.856005E-4	44.0	31
GGTCGAC	25	4.4424356E-5	44.0	9
CGATTCT	50	2.7284841E-11	44.0	10
TCGATCT	35	1.4458419E-7	44.0	16
ACGGGTA	55	1.8189894E-12	44.0	5
CGTTCAC	25	4.4424356E-5	44.0	26
GCGCAAA	20	7.856005E-4	44.0	1
>>END_MODULE